##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551652_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	603513
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9400195190493	33.0	31.0	34.0	30.0	34.0
2	32.031868410456774	33.0	31.0	34.0	30.0	34.0
3	32.00365029419416	33.0	31.0	34.0	30.0	34.0
4	35.699963381070496	37.0	35.0	37.0	33.0	37.0
5	35.716887622967526	37.0	35.0	37.0	35.0	37.0
6	35.591190247765994	37.0	35.0	37.0	33.0	37.0
7	35.89426905468482	37.0	35.0	37.0	35.0	37.0
8	35.593750258900805	37.0	35.0	37.0	33.0	37.0
9	37.397360123145646	39.0	37.0	39.0	34.0	39.0
10	37.12211667354307	39.0	37.0	39.0	34.0	39.0
11	37.20285561371503	39.0	37.0	39.0	34.0	39.0
12	37.140638229831005	39.0	37.0	39.0	34.0	39.0
13	37.16800135208355	39.0	37.0	39.0	34.0	39.0
14	38.26030425193824	40.0	38.0	41.0	33.0	41.0
15	38.26047989024263	40.0	38.0	41.0	33.0	41.0
16	38.0970666746201	40.0	37.0	41.0	33.0	41.0
17	38.05337581791942	40.0	37.0	41.0	33.0	41.0
18	37.86573777201154	39.0	37.0	41.0	33.0	41.0
19	37.69859472786833	39.0	37.0	41.0	33.0	41.0
20	37.838437614434156	40.0	36.0	41.0	33.0	41.0
21	37.79401437914345	39.0	36.0	41.0	33.0	41.0
22	37.95478142144411	40.0	36.0	41.0	34.0	41.0
23	37.931424840889925	40.0	36.0	41.0	33.0	41.0
24	37.79639875197386	40.0	36.0	41.0	33.0	41.0
25	37.491739200315486	39.0	35.0	41.0	33.0	41.0
26	37.66662027164286	39.0	36.0	41.0	33.0	41.0
27	37.70901372464222	40.0	36.0	41.0	33.0	41.0
28	37.56405910063246	40.0	36.0	41.0	33.0	41.0
29	37.618462236936075	40.0	36.0	41.0	33.0	41.0
30	37.344507906209145	39.0	35.0	41.0	33.0	41.0
31	37.27204219296022	39.0	35.0	41.0	32.0	41.0
32	37.215188405220765	40.0	35.0	41.0	32.0	41.0
33	37.10154876531243	40.0	35.0	41.0	32.0	41.0
34	36.98486528044963	40.0	35.0	41.0	32.0	41.0
35	36.92923598994553	40.0	35.0	41.0	31.0	41.0
36	36.70456643021774	39.0	35.0	41.0	31.0	41.0
37	36.77100576126778	39.0	35.0	41.0	31.0	41.0
38	36.520755973773554	39.0	35.0	41.0	31.0	41.0
39	36.45342188155019	39.0	35.0	41.0	31.0	41.0
40	36.36449587664226	39.0	35.0	41.0	30.0	41.0
41	36.16685307524445	39.0	35.0	41.0	30.0	41.0
42	36.274779499364556	39.0	35.0	41.0	30.0	41.0
43	36.18798269465612	39.0	35.0	41.0	30.0	41.0
44	36.30908696250122	39.0	35.0	41.0	31.0	41.0
45	36.27216977927567	39.0	35.0	41.0	31.0	41.0
46	36.16163860596209	39.0	35.0	41.0	31.0	41.0
47	36.04995252794886	38.0	35.0	40.0	30.0	41.0
48	36.023535532788856	38.0	35.0	40.0	30.0	41.0
49	36.01569477376626	38.0	35.0	40.0	30.0	41.0
50	35.85288303648803	38.0	35.0	40.0	30.0	41.0
51	35.048724716783234	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	9.0
10	22.0
11	16.0
12	20.0
13	25.0
14	33.0
15	30.0
16	52.0
17	110.0
18	177.0
19	339.0
20	790.0
21	1318.0
22	2104.0
23	2606.0
24	3408.0
25	4430.0
26	5764.0
27	6601.0
28	7050.0
29	7502.0
30	8865.0
31	10858.0
32	14219.0
33	19971.0
34	35282.0
35	45857.0
36	49876.0
37	74500.0
38	127986.0
39	173625.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95412692021547	18.217006095974735	22.219073988464206	14.609792995345586
2	30.743165433056124	29.52463327219132	23.43478930859816	16.2974119861544
3	29.647745781781005	28.63749413848583	26.259252079077005	15.455508000656156
4	27.018804897326156	23.42766435851423	31.917622321308738	17.635908422850875
5	28.86234430741343	27.03570594171128	24.282824065098847	19.819125685776445
6	25.045359420592433	36.00485822177816	24.39698896295523	14.552793394674183
7	72.87200110022485	5.923981753499925	16.25283962400147	4.9511775222737535
8	72.12106450068184	10.637716171814029	11.425603093885302	5.815616233618829
9	65.97654068760738	7.496441667370878	13.466652748159527	13.060364896862206
10	39.29575667798374	23.629979801594995	20.22392226845155	16.850341251969716
11	30.873734285756893	21.86895725527039	26.630080876468277	20.62722758250444
12	25.658105127810003	19.35716380591636	31.387062084826674	23.59766898144696
13	24.71645184113681	20.57602735980832	34.97836832015218	19.72915247890269
14	21.49879124393344	26.388329663155556	28.926137465141593	23.18674162776941
15	18.801583395883767	22.154452348168142	36.72282121511881	22.321143040829277
16	20.966905435342735	22.413933088433886	29.96522030179963	26.65394117442375
17	20.92912663024657	24.255484140358202	30.294293577768833	24.521095651626393
18	20.95116426655267	23.536692664449646	30.715991204829056	24.796151864168625
19	23.011434716402132	24.20229555949913	27.6375156790326	25.148754045066134
20	24.673702140633257	24.502703338619053	30.00830139533034	20.815293125417348
21	23.286159535917207	27.45574660363571	28.161282358457896	21.096811501989187
22	22.19902471032107	22.200018889402546	28.743042817636073	26.857913582640307
23	23.046562377281017	25.67020097330132	28.372876806299118	22.910359843118542
24	23.26395620309753	23.7822548975747	28.214470939316964	24.739317960010805
25	21.638804797908247	28.6736159784462	26.321719664696534	23.36585955894902
26	21.695141612525333	24.30950120378517	28.85289960613939	25.142457577550108
27	24.88181696168931	24.593505028060704	26.312606356449653	24.212071653800333
28	21.191092818216013	25.215364043525163	29.415604966255906	24.17793817200292
29	25.239224341480632	22.587748731178948	26.205235015650036	25.967791911690384
30	22.587251641638208	26.73612664515926	26.365794937308724	24.31082677589381
31	24.274042149879126	25.558355826635054	23.813571538641256	26.35403048484457
32	25.702014703908617	25.97773370250517	23.66709582063684	24.653155772949383
33	22.868935714723627	25.861083356945088	24.239908668081714	27.03007226024957
34	24.245873742570584	24.7775938546477	26.67167070137677	24.30486170140494
35	24.987365640839553	22.710695544255053	25.078995812849104	27.222943002056294
36	23.88448964645335	27.811497018291238	26.365629240795148	21.938384094460268
37	21.79737636140398	24.812224425985853	28.14073599077402	25.24966322183615
38	22.303744906903415	28.187296711089903	24.929371861086672	24.579586520920014
39	23.0752278741303	23.919948700359395	28.750499160747157	24.254324264763145
40	23.806115195530168	24.371803092891124	29.06316848187197	22.758913229706735
41	21.22489490698626	23.968663475351814	30.09446358239176	24.71197803527016
42	23.752098132103203	23.10936135592771	29.51899959072961	23.619540921239476
43	23.56950057413842	22.857668351800207	28.701121599700418	24.87170947436095
44	22.9978476022886	23.03164969105885	28.518026952194898	25.452475754457648
45	22.060833817995633	22.49910109641383	27.823261470755394	27.61680361483514
46	25.55520759287704	24.48348254304381	26.982848753879367	22.97846111019978
47	21.0111464044685	23.285828142890043	32.4034444991243	23.299580953517157
48	22.114519488395445	23.93154745630997	28.3437142199091	25.610218835385485
49	22.14136232359535	21.722978626806714	31.95970923575797	24.175949813839967
50	21.80234725681137	21.679566140248845	30.345659496978524	26.172427105961265
51	22.14152802010893	21.615772982520674	27.979016193520273	28.263682803850127
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	229.0
1	605.0
2	981.0
3	4453.5
4	7926.0
5	5108.5
6	2291.0
7	2205.0
8	2119.0
9	2097.5
10	2076.0
11	2057.0
12	2038.0
13	2000.0
14	1962.0
15	1825.0
16	1688.0
17	1630.5
18	1573.0
19	1600.5
20	1628.0
21	1758.0
22	1888.0
23	1998.5
24	2109.0
25	2368.0
26	3413.5
27	4200.0
28	5164.5
29	6129.0
30	6708.0
31	7287.0
32	7657.0
33	8027.0
34	9650.5
35	11274.0
36	12390.0
37	13506.0
38	14413.5
39	15321.0
40	17159.5
41	18998.0
42	21717.0
43	24436.0
44	28021.0
45	31606.0
46	53560.0
47	75514.0
48	65516.5
49	55519.0
50	54633.5
51	53748.0
52	47209.0
53	40670.0
54	37116.0
55	33562.0
56	31588.5
57	29615.0
58	28845.0
59	28075.0
60	27139.5
61	26204.0
62	24657.5
63	23111.0
64	20862.5
65	18614.0
66	16071.0
67	13528.0
68	11994.0
69	10460.0
70	9159.5
71	7859.0
72	6842.5
73	5826.0
74	4976.5
75	3309.0
76	2491.0
77	1922.5
78	1354.0
79	1061.5
80	769.0
81	535.0
82	301.0
83	212.5
84	124.0
85	86.0
86	48.0
87	54.0
88	60.0
89	35.5
90	11.0
91	6.5
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	603513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.09015464647672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14070187499566	23.47096434120461
2	8.581733302245611	5.507782976077621
3	3.2174490151741217	3.097453093922754
4	1.843924291757577	2.366872627155829
5	1.370777563307934	2.1994231996236064
6	1.1595764119419336	2.2326591830173945
7	1.0087082275252843	2.2658722110121867
8	0.9180868116551792	2.356923821192436
9	0.8405913711880283	2.4277236386325973
>10	7.797054975221402	42.40943604721051
>50	0.0684798823005184	1.4961516839841769
>100	0.044096893905610585	2.5434745107265853
>500	0.0036315089098738124	0.8035967707342079
>1k	0.003112721922748982	1.8137946586180227
>5k	0.0020751479484993215	5.007871236887447
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	8514	1.4107401166172062	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	8381	1.3887024803111117	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC	7977	1.32176108882493	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5209	0.8631131392364374	No Hit
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	3104	0.5143219781512577	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCT	2013	0.3335470818358511	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCT	1782	0.2952711871989502	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACATGTT	1374	0.2276670096584498	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTC	1363	0.22584434800907355	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTA	1259	0.2086119105967891	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	839	0.13901937489333285	No Hit
CTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGCT	807	0.1337170864587838	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCC	773	0.12808340499707546	No Hit
CCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	703	0.11648464904649941	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTT	636	0.10538298263666233	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6569651357965776E-4	0.0	0.0	0.13918507140691252	0.0
2	1.6569651357965776E-4	0.0	0.0	0.9416532866731951	0.0
3	1.6569651357965776E-4	0.0	0.0	1.2281425586524235	0.0
4	1.6569651357965776E-4	0.0	0.0	1.9391462984227348	0.0
5	1.6569651357965776E-4	0.0	0.0	4.419954499737371	0.0
6	1.6569651357965776E-4	0.0	0.0	5.4343485558720355	0.0
7	3.313930271593155E-4	0.0	0.0	6.539212908421194	0.0
8	3.313930271593155E-4	0.0	0.0	8.06594058454416	0.0
9	3.313930271593155E-4	0.0	0.0	8.699232659445613	0.0
10	3.313930271593155E-4	0.0	0.0	11.176229840947917	0.0
11	3.313930271593155E-4	0.0	0.0	12.392939340163343	0.0
12	3.313930271593155E-4	0.0	0.0	14.795373090554802	0.0
13	3.313930271593155E-4	0.0	0.0	15.29527947202463	0.0
14	3.313930271593155E-4	0.0	0.0	15.533551058552177	0.0
15	3.313930271593155E-4	0.0	0.0	16.042239355241726	0.0
16	3.313930271593155E-4	0.0	0.0	16.667080907950616	0.0
17	3.313930271593155E-4	0.0	0.0	17.354058653251876	0.0
18	3.313930271593155E-4	0.0	0.0	18.097041820143062	0.0
19	3.313930271593155E-4	0.0	0.0	18.777226008387558	0.0
20	3.313930271593155E-4	0.0	0.0	19.24449017668219	0.0
21	3.313930271593155E-4	0.0	0.0	19.736940215040935	0.0
22	3.313930271593155E-4	0.0	0.0	20.27230565041681	0.0
23	3.313930271593155E-4	0.0	0.0	20.787621807649547	0.0
24	3.313930271593155E-4	0.0	0.0	21.203520056734487	0.0
25	4.970895407389733E-4	0.0	0.0	21.576999998343034	0.0
26	4.970895407389733E-4	0.0	0.0	21.90325643358138	0.0
27	4.970895407389733E-4	0.0	0.0	22.259503937777644	0.0
28	4.970895407389733E-4	0.0	0.0	22.600341666211	0.0
29	4.970895407389733E-4	0.0	0.0	22.967525140303522	0.0
30	6.62786054318631E-4	0.0	0.0	23.37016766830209	0.0
31	9.941790814779466E-4	0.0	0.0	23.73370581909586	0.0
32	9.941790814779466E-4	0.0	0.0	24.099398024566167	0.0
33	9.941790814779466E-4	0.0	0.0	24.45050893684146	0.0
34	9.941790814779466E-4	0.0	0.0	24.796980346736525	0.0
35	9.941790814779466E-4	0.0	0.0	25.16151267661177	0.0
36	9.941790814779466E-4	0.0	0.0	25.512789285400647	0.0
37	9.941790814779466E-4	0.0	0.0	25.851472959157466	0.0
38	9.941790814779466E-4	0.0	0.0	26.196453100430315	0.0
39	9.941790814779466E-4	0.0	0.0	26.539113490513046	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGATG	25	3.888595E-5	45.000004	1
ACGATCG	50	2.1827873E-11	45.000004	40
CGATCGA	85	0.0	45.000004	41
TGATCGG	20	7.0306216E-4	45.0	2
GCAACCG	20	7.0306216E-4	45.0	1
GTATGCG	20	7.0306216E-4	45.0	1
CGTAAGC	20	7.0306216E-4	45.0	43
TACAACG	20	7.0306216E-4	45.0	1
CTCACGA	30	2.1637825E-6	44.999996	24
CGATCAC	30	2.1637825E-6	44.999996	34
CGAATGC	85	0.0	42.352943	45
CGTTTTT	2215	0.0	41.241535	1
CGTATGG	105	0.0	40.714287	2
GCGAATG	50	1.0804797E-9	40.500004	1
TACGGCT	905	0.0	40.276245	7
GATGAAT	735	0.0	40.10204	20
CCGATGA	660	0.0	39.886364	18
CGATGAA	700	0.0	39.535713	19
CCGATCG	40	3.4545883E-7	39.375	40
TATTAGT	120	0.0	39.374996	30
>>END_MODULE