FastQCFastQC Report
Sat 18 Jun 2016
SRR3551651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427956
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC60491.413463066296535No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCG57741.349204123788427No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC49421.1547916140911683No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41130.9610801110394527No Hit
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC22900.5351017394311565Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGAGAGGT13570.3170886726672836No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCT12550.29325444671882156No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTA10320.2411462860667919No Hit
GAACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT10220.23880959724831524Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTC9170.21427436465431024No Hit
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC9130.2133396891269196Illumina Single End Adapter 1 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT8670.20259092056192693Illumina Single End Adapter 1 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8470.1979175429249736No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC6610.1544551309013076No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5070.11847012309676695No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5030.1175354475693763No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4780.11169372552318463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACCG406.7975634E-945.00000436
TACGACG207.028318E-445.0000041
GGTATCG207.028318E-445.0000041
TATAGCG207.028318E-445.0000041
TGTACCG207.028318E-445.0000041
GTTATCG207.028318E-445.0000041
TCAAACC207.028318E-445.00000415
CAAACGG351.209537E-745.02
ACGTAGG253.8866878E-545.02
CGGTCGA351.209537E-745.041
CGTTTTT18800.042.127661
CATACGA650.041.53846418
CGAATAT1150.041.0869614
GCTACGA1150.041.0869610
TACGGCT7300.040.9931537
GTCGAAT556.002665E-1140.90909243
GGTCGAA556.002665E-1140.90909242
AACGGGA950.040.2631574
ACGGCTG7550.039.635768
GAATGAC1650.039.5454521
Produced by FastQC (version 0.11.5)