##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551651_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427956
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04510043088542	33.0	31.0	34.0	30.0	34.0
2	32.147688080083	33.0	31.0	34.0	30.0	34.0
3	32.10857658263934	33.0	31.0	34.0	30.0	34.0
4	35.78590789707353	37.0	35.0	37.0	35.0	37.0
5	35.81192459037845	37.0	35.0	37.0	35.0	37.0
6	35.70728532839825	37.0	35.0	37.0	35.0	37.0
7	35.982201441269666	37.0	35.0	37.0	35.0	37.0
8	35.68428530035798	37.0	35.0	37.0	35.0	37.0
9	37.50774612343325	39.0	37.0	39.0	35.0	39.0
10	37.28919094486349	39.0	37.0	39.0	34.0	39.0
11	37.36318453298937	39.0	37.0	39.0	34.0	39.0
12	37.26543616633486	39.0	37.0	39.0	34.0	39.0
13	37.287361317518624	39.0	37.0	39.0	34.0	39.0
14	38.36229892792717	40.0	38.0	41.0	34.0	41.0
15	38.35696894073222	40.0	38.0	41.0	34.0	41.0
16	38.23184392788043	40.0	37.0	41.0	34.0	41.0
17	38.199721466692836	40.0	37.0	41.0	34.0	41.0
18	38.02836272887867	40.0	37.0	41.0	33.0	41.0
19	37.80797091289759	39.0	37.0	41.0	33.0	41.0
20	37.92515819383301	40.0	36.0	41.0	34.0	41.0
21	37.88818242996943	40.0	36.0	41.0	34.0	41.0
22	38.051823084616174	40.0	36.0	41.0	34.0	41.0
23	38.04604211647926	40.0	36.0	41.0	34.0	41.0
24	37.930013833197805	40.0	36.0	41.0	34.0	41.0
25	37.622596715550195	39.0	35.0	41.0	33.0	41.0
26	37.80093046948752	40.0	36.0	41.0	34.0	41.0
27	37.84941208909327	40.0	36.0	41.0	34.0	41.0
28	37.74251558571442	40.0	36.0	41.0	33.0	41.0
29	37.79276607875576	40.0	36.0	41.0	33.0	41.0
30	37.539796147267474	40.0	35.0	41.0	33.0	41.0
31	37.41064735627027	39.0	35.0	41.0	33.0	41.0
32	37.30831674284272	40.0	35.0	41.0	33.0	41.0
33	37.18988400676705	40.0	35.0	41.0	33.0	41.0
34	36.922342483806744	40.0	35.0	41.0	32.0	41.0
35	36.87478151959547	40.0	35.0	41.0	32.0	41.0
36	36.68559851947396	39.0	35.0	41.0	31.0	41.0
37	36.54894662068063	39.0	35.0	41.0	31.0	41.0
38	36.42276542448289	39.0	35.0	41.0	31.0	41.0
39	36.35210629130098	39.0	35.0	41.0	30.0	41.0
40	36.26482629055324	39.0	35.0	41.0	30.0	41.0
41	36.079543224069766	39.0	35.0	41.0	30.0	41.0
42	36.11913841609885	39.0	35.0	41.0	30.0	41.0
43	36.08474469337969	39.0	35.0	41.0	30.0	41.0
44	36.12952032451934	39.0	35.0	41.0	30.0	41.0
45	36.14825823215471	39.0	35.0	41.0	31.0	41.0
46	36.09806615633383	39.0	35.0	41.0	31.0	41.0
47	35.96876781725224	38.0	35.0	40.0	30.0	41.0
48	35.97705838918019	38.0	35.0	40.0	30.0	41.0
49	35.963458860256665	38.0	35.0	40.0	30.0	41.0
50	35.8108941106095	38.0	35.0	40.0	30.0	41.0
51	34.96764620661937	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	10.0
10	12.0
11	13.0
12	10.0
13	22.0
14	18.0
15	17.0
16	30.0
17	53.0
18	114.0
19	206.0
20	439.0
21	860.0
22	1286.0
23	1601.0
24	2154.0
25	3114.0
26	4201.0
27	5104.0
28	5347.0
29	5181.0
30	5863.0
31	7371.0
32	9487.0
33	13488.0
34	25449.0
35	33802.0
36	34429.0
37	51752.0
38	90713.0
39	125761.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.92424454850499	17.912355475796577	21.905756666573197	15.257643309125237
2	31.601846918842124	29.235248483488956	22.6915851162269	16.471319481442016
3	29.66730224602529	28.098916711063755	27.611249754647677	14.62253128826328
4	27.371271812990123	22.42847395526643	32.849638747908664	17.350615483834787
5	28.598267111572216	26.707652188542745	25.255867425623197	19.43821327426184
6	24.738057183448767	35.10734748432082	25.708250380880276	14.44634495135014
7	71.59240669601547	6.049921019917936	17.033526811167505	5.324145472899083
8	70.44415781061605	10.680303582611296	12.834029666601241	6.041508940171419
9	64.2696912766733	8.214162203591023	14.749880828870257	12.766265690865417
10	39.94592902074045	22.271915804428495	21.317144753198928	16.46501042163213
11	31.32284627391601	22.230556412341457	26.228163642991337	20.2184336707512
12	25.70614736094365	19.615100617820524	31.32892166484405	23.34983035639178
13	24.41793081531746	21.58235893409603	34.985605996878185	19.014104253708325
14	20.779939993831142	27.147417024180054	29.804465879669873	22.26817710231893
15	18.40586415425885	22.367019039340494	37.22532222938807	22.00179457701259
16	19.805073418762674	23.049565843217525	31.25928833805344	25.886072399966352
17	20.572442026750416	23.87722102272196	31.281253212947124	24.269083737580498
18	21.32462215741805	22.693220798399835	31.698585835927055	24.283571208255054
19	22.60162259671555	24.222583630092814	28.628644066212416	24.54714970697922
20	24.173045827141106	24.57612464832833	30.916028750619223	20.334800773911336
21	22.85912570451168	27.779725018459843	28.587284674125378	20.773864602903103
22	21.072960771668114	22.760050098608268	29.118180373683277	27.048808756040337
23	21.591472020488087	26.07464318761742	29.17262522315378	23.16125956874071
24	22.72476609744927	23.903859275252596	28.455495424763296	24.91587920253484
25	20.56706764246792	28.82001888044565	27.65097346456178	22.96194001252465
26	20.095991176663023	24.54738337586107	30.877473385114357	24.47915206236155
27	23.984007701726345	24.37283272112086	28.88661451177224	22.756545065380553
28	20.321715316527865	25.206329622671493	31.620774098271788	22.851180962528858
29	22.056473095364947	23.319219732869733	30.943134340913552	23.68117283085177
30	22.904924805353822	24.779416575535805	30.760171606426827	21.555487012683546
31	25.64375776949032	24.359513594855546	25.94425595154642	24.052472684107713
32	25.376440568656587	27.804260251053847	26.26111095533186	20.558188224957703
33	26.559973455215026	23.682808513024703	25.017525166138576	24.7396928656217
34	23.870911962912075	26.098010075802186	27.903335856957256	22.127742104328483
35	25.91808503677948	23.473207526007347	27.76804157436746	22.840665862845714
36	27.69607155875838	25.32830477899597	26.842712802250702	20.132910859994954
37	23.564572058809784	27.34580190486873	29.1756629186178	19.91396311770369
38	27.358887362252194	26.287749207862493	24.318621540532202	22.03474188935311
39	26.101281440148057	27.00581368178037	25.98234397928759	20.910560898783988
40	26.47982502874127	23.586303264821616	28.592191720644177	21.341679985792933
41	24.388488536204655	25.316621334903587	25.755685163895354	24.539204964996404
42	25.493275009580422	23.347259998691456	29.582013104150896	21.57745188757723
43	27.226397106244566	21.95482713176121	27.860340782697286	22.95843497929694
44	24.18192524465132	23.09723429511445	29.066305881913095	23.654534578321133
45	23.737487031377057	22.013244352223126	27.7991195356532	26.45014908074662
46	26.779622204151828	24.990419575844243	26.695735075568518	21.534223144435412
47	22.038013253698978	23.782117787809963	32.573442129564725	21.606426828926338
48	23.940077951938985	24.227257007729765	27.648169437979604	24.184495602351642
49	23.94218097187561	21.91744011066558	31.67264859004197	22.467730327416838
50	23.818803802260046	21.7807438147847	29.562151249193843	24.838301133761416
51	22.945349521913467	22.128676779855873	26.832898709213097	28.093074989017563
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	243.0
1	468.0
2	693.0
3	3645.0
4	6597.0
5	4385.0
6	2173.0
7	2064.0
8	1955.0
9	1964.0
10	1973.0
11	1958.5
12	1944.0
13	1910.5
14	1877.0
15	1721.5
16	1566.0
17	1521.5
18	1477.0
19	1362.5
20	1248.0
21	1322.0
22	1396.0
23	1446.5
24	1497.0
25	1817.5
26	2609.5
27	3081.0
28	3635.0
29	4189.0
30	4967.5
31	5746.0
32	6395.0
33	7044.0
34	7752.0
35	8460.0
36	8633.5
37	8807.0
38	9104.0
39	9401.0
40	11557.0
41	13713.0
42	15523.0
43	17333.0
44	19585.0
45	21837.0
46	37755.5
47	53674.0
48	47474.0
49	41274.0
50	40773.5
51	40273.0
52	34451.5
53	28630.0
54	25827.0
55	23024.0
56	21574.0
57	20124.0
58	19047.5
59	17971.0
60	17599.0
61	17227.0
62	16084.0
63	14941.0
64	13581.0
65	12221.0
66	10716.5
67	9212.0
68	8045.0
69	6878.0
70	5995.0
71	5112.0
72	4647.5
73	4183.0
74	3672.5
75	2405.0
76	1648.0
77	1339.0
78	1030.0
79	746.5
80	463.0
81	415.0
82	367.0
83	237.5
84	108.0
85	75.0
86	42.0
87	22.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	427956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.997263536595334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.67511125363218	25.047521733777145
2	9.465666871696712	6.4361354237338615
3	3.4110815646209285	3.4790231669171896
4	1.8403836274980128	2.50272028769941
5	1.256891685759752	2.136543893886493
6	1.0386881317084926	2.118753248761648
7	0.8771611781453008	2.087475581623337
8	0.7547138049113857	2.052656329622318
9	0.7178219660277387	2.1963584296261818
>10	6.836770223713656	39.96564534650725
>50	0.07252482247261574	1.6566789543299247
>100	0.04213346829361486	2.5598096784054585
>500	0.00483498816484105	1.2246039329313336
>1k	0.00483498816484105	3.7597571563113297
>5k	0.0013814251899545856	2.776316835867144
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC	6049	1.413463066296535	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCG	5774	1.349204123788427	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC	4942	1.1547916140911683	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4113	0.9610801110394527	No Hit
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	2290	0.5351017394311565	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGAGAGGT	1357	0.3170886726672836	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCT	1255	0.29325444671882156	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTA	1032	0.2411462860667919	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT	1022	0.23880959724831524	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTC	917	0.21427436465431024	No Hit
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	913	0.2133396891269196	Illumina Single End Adapter 1 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT	867	0.20259092056192693	Illumina Single End Adapter 1 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	847	0.1979175429249736	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC	661	0.1544551309013076	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	507	0.11847012309676695	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	503	0.1175354475693763	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	478	0.11169372552318463	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2091336492536616	0.0
2	0.0	0.0	0.0	1.169045415883876	0.0
3	0.0	0.0	0.0	1.5190814008916804	0.0
4	0.0	0.0	0.0	2.2324724971726067	0.0
5	0.0	0.0	0.0	4.648375066595631	0.0
6	0.0	0.0	0.0	5.61482956191758	0.0
7	0.0	0.0	0.0	6.806307190458832	0.0
8	0.0	0.0	0.0	8.424931535017619	0.0
9	0.0	0.0	0.0	9.096028563684117	0.0
10	0.0	0.0	0.0	11.682743085737787	0.0
11	0.0	0.0	0.0	13.313751881034499	0.0
12	0.0	0.0	0.0	16.100720634831617	0.0
13	0.0	0.0	0.0	16.729056258119993	0.0
14	0.0	0.0	0.0	16.9938031012534	0.0
15	0.0	0.0	0.0	17.629382459879054	0.0
16	0.0	0.0	0.0	18.414276234005364	0.0
17	0.0	0.0	0.0	19.31623811793736	0.0
18	0.0	0.0	0.0	20.236192505771623	0.0
19	0.0	0.0	0.0	21.130209647720793	0.0
20	0.0	0.0	0.0	21.708773799175617	0.0
21	0.0	0.0	0.0	22.317481236388787	0.0
22	0.0	0.0	0.0	22.997691351447344	0.0
23	2.3366888184766659E-4	0.0	0.0	23.60920281524269	0.0
24	2.3366888184766659E-4	0.0	0.0	24.11976932207984	0.0
25	2.3366888184766659E-4	0.0	0.0	24.558833151071607	0.0
26	2.3366888184766659E-4	0.0	0.0	24.965417005486547	0.0
27	2.3366888184766659E-4	0.0	0.0	25.42854872930862	0.0
28	2.3366888184766659E-4	0.0	0.0	25.835833590369102	0.0
29	4.6733776369533317E-4	0.0	0.0	26.246389815775455	0.0
30	4.6733776369533317E-4	0.0	0.0	26.72961706343643	0.0
31	4.6733776369533317E-4	0.0	0.0	27.159567806036133	0.0
32	4.6733776369533317E-4	0.0	0.0	27.60470702595594	0.0
33	4.6733776369533317E-4	0.0	0.0	28.002411462860668	0.0
34	4.6733776369533317E-4	0.0	0.0	28.419510416958754	0.0
35	4.6733776369533317E-4	0.0	0.0	28.858807914832365	0.0
36	4.6733776369533317E-4	0.0	0.0	29.25277364962753	0.0
37	4.6733776369533317E-4	0.0	0.0	29.661226855097254	0.0
38	4.6733776369533317E-4	0.0	0.0	30.08323285571414	0.0
39	4.6733776369533317E-4	0.0	0.0	30.49846245875744	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACCG	40	6.7975634E-9	45.000004	36
TACGACG	20	7.028318E-4	45.000004	1
GGTATCG	20	7.028318E-4	45.000004	1
TATAGCG	20	7.028318E-4	45.000004	1
TGTACCG	20	7.028318E-4	45.000004	1
GTTATCG	20	7.028318E-4	45.000004	1
TCAAACC	20	7.028318E-4	45.000004	15
CAAACGG	35	1.209537E-7	45.0	2
ACGTAGG	25	3.8866878E-5	45.0	2
CGGTCGA	35	1.209537E-7	45.0	41
CGTTTTT	1880	0.0	42.12766	1
CATACGA	65	0.0	41.538464	18
CGAATAT	115	0.0	41.08696	14
GCTACGA	115	0.0	41.08696	10
TACGGCT	730	0.0	40.993153	7
GTCGAAT	55	6.002665E-11	40.909092	43
GGTCGAA	55	6.002665E-11	40.909092	42
AACGGGA	95	0.0	40.263157	4
ACGGCTG	755	0.0	39.63576	8
GAATGAC	165	0.0	39.545452	1
>>END_MODULE