FastQCFastQC Report
Sat 18 Jun 2016
SRR3551649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269988
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC42741.5830333199994076No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG38021.408210735291939No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC33631.245610916040713No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC19100.7074388491340355No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.48928100508170735No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGTCTT11000.4074255152080833No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT8050.2981613997659155No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC7750.2870497948056951No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTA7080.2622338770612027No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT6760.25038149843696755No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT6270.2322325436686075No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5750.21297242840422537No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTT5670.21000933374816658No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTC5320.1970457946279094No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC3380.12519074921848378No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3210.11889417307435887No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC2860.10593063395410167No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2750.10185637880202082No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA2730.10111560513800613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT750.045.00000414
GCTACGA700.045.00000410
TTAGGCG253.882851E-545.01
GATACGT207.0236897E-445.01
CATATAA207.0236897E-445.017
CGTATGG207.0236897E-445.02
CGACGGT253.882851E-545.028
CGACAAA207.0236897E-445.012
AATTGCA253.882851E-545.031
CGAATGG207.0236897E-445.02
ATTGGCA207.0236897E-445.012
CTCGAAT253.882851E-545.043
ATACGTT207.0236897E-445.02
CGATCAC207.0236897E-445.034
GACCTGC207.0236897E-445.037
AGTACGG207.0236897E-445.02
ATATTAG406.7811925E-945.029
GGCGTAG302.1593114E-644.9999961
TACGGGT302.1593114E-644.9999964
ATGTAGG302.1593114E-644.9999962
Produced by FastQC (version 0.11.5)