##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551649_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269988
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.948934767471147	33.0	31.0	34.0	30.0	34.0
2	32.03849430345053	33.0	31.0	34.0	30.0	34.0
3	32.016426655999524	33.0	31.0	34.0	30.0	34.0
4	35.69836066788154	37.0	35.0	37.0	33.0	37.0
5	35.69549016993348	37.0	35.0	37.0	35.0	37.0
6	35.577188615790334	37.0	35.0	37.0	33.0	37.0
7	35.85148969583833	37.0	35.0	37.0	35.0	37.0
8	35.52508630013186	37.0	35.0	37.0	33.0	37.0
9	37.270471280205044	39.0	37.0	39.0	34.0	39.0
10	37.0622360993822	39.0	37.0	39.0	34.0	39.0
11	37.17442997466554	39.0	37.0	39.0	34.0	39.0
12	37.063191697408776	39.0	37.0	39.0	34.0	39.0
13	37.07630339126183	39.0	37.0	39.0	33.0	39.0
14	38.13187623153622	40.0	37.0	41.0	33.0	41.0
15	38.14037290546246	40.0	37.0	41.0	33.0	41.0
16	38.00600397054684	40.0	37.0	41.0	33.0	41.0
17	37.93128953879432	40.0	37.0	41.0	33.0	41.0
18	37.78790909225595	39.0	36.0	41.0	33.0	41.0
19	37.62259063365779	39.0	36.0	41.0	33.0	41.0
20	37.7204579462791	39.0	36.0	41.0	33.0	41.0
21	37.69053809798954	39.0	36.0	41.0	33.0	41.0
22	37.82837755752107	40.0	36.0	41.0	33.0	41.0
23	37.8157066239981	40.0	36.0	41.0	33.0	41.0
24	37.72551372653599	40.0	36.0	41.0	33.0	41.0
25	37.4235373424004	39.0	35.0	41.0	33.0	41.0
26	37.563843578233104	39.0	36.0	41.0	33.0	41.0
27	37.564299154036476	40.0	36.0	41.0	33.0	41.0
28	37.39976221165385	40.0	35.0	41.0	33.0	41.0
29	37.47841755929893	40.0	36.0	41.0	33.0	41.0
30	37.24754803917211	39.0	35.0	41.0	32.0	41.0
31	37.129113145769445	39.0	35.0	41.0	32.0	41.0
32	37.042642635969	39.0	35.0	41.0	31.0	41.0
33	36.97929537609079	40.0	35.0	41.0	31.0	41.0
34	36.82595893150807	40.0	35.0	41.0	31.0	41.0
35	36.792620412759085	40.0	35.0	41.0	31.0	41.0
36	36.55927670859445	39.0	35.0	41.0	30.0	41.0
37	36.54483162214617	39.0	35.0	41.0	31.0	41.0
38	36.357041794450126	39.0	35.0	41.0	30.0	41.0
39	36.3153177178245	39.0	35.0	41.0	30.0	41.0
40	36.31252129724284	39.0	35.0	41.0	30.0	41.0
41	36.108038135028224	39.0	35.0	41.0	29.0	41.0
42	36.16222943241922	39.0	35.0	41.0	30.0	41.0
43	36.12380550246677	39.0	35.0	41.0	30.0	41.0
44	36.21313910247863	39.0	35.0	41.0	30.0	41.0
45	36.14516571106864	39.0	35.0	41.0	30.0	41.0
46	36.069695690178825	39.0	35.0	41.0	30.0	41.0
47	35.9392380402092	38.0	35.0	40.0	29.0	41.0
48	35.915088818762314	38.0	35.0	40.0	30.0	41.0
49	35.89876957494407	38.0	35.0	40.0	30.0	41.0
50	35.739203223846985	38.0	35.0	40.0	29.0	41.0
51	34.90435500837074	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	12.0
11	7.0
12	5.0
13	6.0
14	13.0
15	25.0
16	24.0
17	45.0
18	83.0
19	210.0
20	491.0
21	836.0
22	1182.0
23	1557.0
24	1927.0
25	2279.0
26	2629.0
27	2836.0
28	3004.0
29	3368.0
30	3978.0
31	4982.0
32	6530.0
33	9155.0
34	16610.0
35	20669.0
36	20882.0
37	31913.0
38	55895.0
39	78797.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.572440256604	17.957464776212277	21.16538512822792	14.304709838955807
2	29.557980354682428	31.257315139932146	22.628783501488954	16.555921003896472
3	29.4053809798954	30.062447219876436	25.216305909892288	15.315865890335868
4	27.634931922900275	22.503222365438464	32.087352030460615	17.774493681200646
5	30.183934100774852	26.22375809295228	23.774389972887683	19.817917833385188
6	23.92847089500274	37.551298576233016	23.556602515667365	14.963628013096878
7	70.58535934930441	6.191386283834837	17.8982028830911	5.3250514837696485
8	69.63457635154155	12.765382165133266	11.268648976991571	6.331392506333615
9	63.30540616619999	8.434078551639331	13.394299005881743	14.866216276278946
10	40.06659555239492	23.10732328844245	20.142747085055632	16.683334074106998
11	33.38852097130243	21.823932915536986	25.39705468391188	19.39049142924871
12	26.47376920455724	18.873061024934444	30.423203994251597	24.229965776256723
13	25.80299865179193	22.17802272693601	32.853682385883815	19.16529623538824
14	20.432759974517385	28.646087974280338	28.00346682074759	22.917685230454687
15	18.301554143147104	22.81916233314073	36.21494288635051	22.66434063736166
16	19.853104582425885	22.649154777249358	30.11652369734951	27.381216942975247
17	20.694253077914574	24.98036949790361	29.94244188630606	24.382935537875756
18	20.848704386861638	22.477665674029957	30.737292027793828	25.936337911314578
19	22.519519386046788	23.766241462583523	27.5252974206261	26.188941730743586
20	24.829251670444613	24.17477813828763	30.710994562721304	20.284975628546455
21	23.18140065484392	29.020549061439766	27.702342326325613	20.095707957390697
22	21.046120568321555	23.449190334385232	29.735395647213952	25.76929345007926
23	22.423218809724876	26.614145813888022	27.930130228010135	23.032505148376963
24	22.515074744062698	24.926663407262545	27.341955938782466	25.216305909892288
25	20.4183148880691	30.25764108034431	25.682993318221552	23.64105071336504
26	20.922411366431103	24.77073055098745	29.14722135798628	25.15963672459517
27	25.288531342133723	25.42927833829652	26.511919048253997	22.77027127131576
28	19.911255315051037	26.049676281908823	31.126198201401543	22.91287020163859
29	24.981480658399633	22.047646562069424	29.683171103900914	23.28770167563003
30	24.914811028638308	26.77674563313925	27.31936234203002	20.989080996192424
31	24.465531801413395	23.138435782331065	27.505296531697702	24.89073588455783
32	26.80341348504378	25.45113116138495	26.605997303583862	21.139458049987407
33	26.644887920944633	22.455812850941523	26.397469517163724	24.501829710950116
34	24.885920855741738	22.53952027497518	29.028697571743926	23.54586129753915
35	26.452286768300816	21.518734165962933	26.71489103219402	25.31408803354223
36	25.37705379498348	24.34663762833904	29.153147547298396	21.123161029379084
37	23.404003140880334	22.86323836614961	32.84108923359557	20.89166925937449
38	22.579522052831976	24.139591389246927	28.971287612782792	24.3095989451383
39	23.538083174066994	22.778049394787917	32.358845578322	21.325021852823088
40	25.12444997555447	21.378357556632146	28.84980073188438	24.647391735929002
41	20.899447382846645	22.825088522452848	30.209861179015363	26.065602915685144
42	21.569847548779947	22.019497162836867	31.21101678593123	25.19963850245196
43	24.946664296190942	21.455398017689674	29.703542379661318	23.894395306458065
44	23.740684771175015	22.01060787886869	30.469502348252515	23.77920500170378
45	22.620634991184794	21.399099219224556	28.971287612782792	27.008978176807858
46	25.645584248188808	24.2388550602249	28.192734491903344	21.922826199682948
47	21.530586544587166	23.071395765737737	33.22184689689912	22.176170792775977
48	22.05246159088552	23.006948456968455	29.988369853474968	24.952220098671052
49	22.140984043735276	20.706105456538808	33.21851341541105	23.93439708431486
50	22.793235254900218	21.462435367497815	30.64802880128006	25.096300576321912
51	22.212098315480688	21.138717276323394	27.929759841178125	28.719424567017793
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116.0
1	334.0
2	552.0
3	1436.5
4	2321.0
5	1617.5
6	914.0
7	902.5
8	891.0
9	876.5
10	862.0
11	803.5
12	745.0
13	778.5
14	812.0
15	831.0
16	850.0
17	856.0
18	862.0
19	823.0
20	784.0
21	831.5
22	879.0
23	883.5
24	888.0
25	1020.5
26	1502.5
27	1852.0
28	2210.5
29	2569.0
30	3190.0
31	3811.0
32	4005.0
33	4199.0
34	4835.5
35	5472.0
36	5620.5
37	5769.0
38	6279.5
39	6790.0
40	8228.5
41	9667.0
42	10789.0
43	11911.0
44	13297.5
45	14684.0
46	24437.0
47	34190.0
48	30068.5
49	25947.0
50	26241.0
51	26535.0
52	22701.0
53	18867.0
54	17000.5
55	15134.0
56	14476.5
57	13819.0
58	12648.5
59	11478.0
60	10974.0
61	10470.0
62	10171.0
63	9872.0
64	8707.5
65	7543.0
66	6463.0
67	5383.0
68	4772.0
69	4161.0
70	3576.0
71	2991.0
72	2438.0
73	1885.0
74	1495.5
75	886.5
76	667.0
77	514.0
78	361.0
79	242.5
80	124.0
81	85.5
82	47.0
83	46.0
84	45.0
85	26.0
86	7.0
87	4.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	269988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.7390402536409
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.10954622899256	27.227136020860186
2	8.053149983365426	5.917300028149399
3	3.1424221955620975	3.4634872661007154
4	2.0274016795878658	2.9793916766671114
5	1.549535744170338	2.846422803976473
6	1.4950953211480882	3.295702031201387
7	1.1825669667610972	3.041246277612338
8	1.1613956911413335	3.4134850437797235
9	1.0192456976943474	3.370149784434864
>10	6.164873829278866	33.51000785220084
>50	0.05343226704035649	1.3430226528586455
>100	0.027220211511124998	1.801561550883743
>500	0.008065247855148148	1.9500866705186897
>1k	0.006048935891361112	5.841000340755886
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC	4274	1.5830333199994076	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG	3802	1.408210735291939	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC	3363	1.245610916040713	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	1910	0.7074388491340355	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1321	0.48928100508170735	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGTCTT	1100	0.4074255152080833	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT	805	0.2981613997659155	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	775	0.2870497948056951	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTA	708	0.2622338770612027	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT	676	0.25038149843696755	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	627	0.2322325436686075	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	575	0.21297242840422537	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTT	567	0.21000933374816658	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTC	532	0.1970457946279094	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC	338	0.12519074921848378	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	321	0.11889417307435887	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	286	0.10593063395410167	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	275	0.10185637880202082	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	273	0.10111560513800613	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2400106671407618	0.0
2	0.0	0.0	0.0	1.4559906366208868	0.0
3	0.0	0.0	0.0	1.7863756907714416	0.0
4	0.0	0.0	0.0	2.4486273464005808	0.0
5	0.0	0.0	0.0	4.831325836703853	0.0
6	0.0	0.0	0.0	5.799146628739055	0.0
7	0.0	0.0	0.0	6.768078581270279	0.0
8	0.0	0.0	0.0	8.072210616768153	0.0
9	0.0	0.0	0.0	8.605567654858735	0.0
10	0.0	0.0	0.0	11.111604960220454	0.0
11	0.0	0.0	0.0	12.562039794361231	0.0
12	0.0	0.0	0.0	15.359941923344742	0.0
13	0.0	0.0	0.0	15.972191356652889	0.0
14	0.0	0.0	0.0	16.243684904514275	0.0
15	3.703868320073485E-4	0.0	0.0	16.86741632961465	0.0
16	3.703868320073485E-4	0.0	0.0	17.584855623212885	0.0
17	3.703868320073485E-4	0.0	0.0	18.343037468331925	0.0
18	3.703868320073485E-4	0.0	0.0	19.134924515163636	0.0
19	3.703868320073485E-4	0.0	0.0	20.02570484614131	0.0
20	3.703868320073485E-4	0.0	0.0	20.54535757144762	0.0
21	3.703868320073485E-4	0.0	0.0	21.059824881105826	0.0
22	3.703868320073485E-4	0.0	0.0	21.592441145532394	0.0
23	3.703868320073485E-4	0.0	0.0	22.10024149221447	0.0
24	3.703868320073485E-4	0.0	0.0	22.502851978606458	0.0
25	3.703868320073485E-4	0.0	0.0	22.901017823014357	0.0
26	3.703868320073485E-4	0.0	0.0	23.279923552157875	0.0
27	3.703868320073485E-4	0.0	0.0	23.696238351334134	0.0
28	3.703868320073485E-4	0.0	0.0	24.055513578381262	0.0
29	7.40773664014697E-4	0.0	0.0	24.412566484436347	0.0
30	7.40773664014697E-4	0.0	0.0	24.795916855563952	0.0
31	7.40773664014697E-4	0.0	0.0	25.152599374787027	0.0
32	7.40773664014697E-4	0.0	0.0	25.51668963065025	0.0
33	7.40773664014697E-4	0.0	0.0	25.866705186897196	0.0
34	7.40773664014697E-4	0.0	0.0	26.225980413944324	0.0
35	7.40773664014697E-4	0.0	0.0	26.5774775175193	0.0
36	7.40773664014697E-4	0.0	0.0	26.91786301613405	0.0
37	7.40773664014697E-4	0.0	0.0	27.271582440701067	0.0
38	7.40773664014697E-4	0.0	0.0	27.60493058950768	0.0
39	7.40773664014697E-4	0.0	0.0	27.933093322666192	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	75	0.0	45.000004	14
GCTACGA	70	0.0	45.000004	10
TTAGGCG	25	3.882851E-5	45.0	1
GATACGT	20	7.0236897E-4	45.0	1
CATATAA	20	7.0236897E-4	45.0	17
CGTATGG	20	7.0236897E-4	45.0	2
CGACGGT	25	3.882851E-5	45.0	28
CGACAAA	20	7.0236897E-4	45.0	12
AATTGCA	25	3.882851E-5	45.0	31
CGAATGG	20	7.0236897E-4	45.0	2
ATTGGCA	20	7.0236897E-4	45.0	12
CTCGAAT	25	3.882851E-5	45.0	43
ATACGTT	20	7.0236897E-4	45.0	2
CGATCAC	20	7.0236897E-4	45.0	34
GACCTGC	20	7.0236897E-4	45.0	37
AGTACGG	20	7.0236897E-4	45.0	2
ATATTAG	40	6.7811925E-9	45.0	29
GGCGTAG	30	2.1593114E-6	44.999996	1
TACGGGT	30	2.1593114E-6	44.999996	4
ATGTAGG	30	2.1593114E-6	44.999996	2
>>END_MODULE