FastQCFastQC Report
Sat 18 Jun 2016
SRR3551644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450492
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36590.8122230805430506No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGC22560.5007858075171145No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTC20880.4634932473828614No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCG20680.45905365689068844No Hit
GCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC13270.29456682915567867No Hit
CTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGCT8170.18135727160526713Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC7820.17358798824396438No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCC7080.15716150342292426No Hit
GAACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCT4580.10166662227076173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCGT302.162551E-645.00000434
TCGTTCG207.0287153E-445.045
TCCATAT253.887016E-545.043
CGAAAGG351.2097371E-745.02
TAGACAC207.0287153E-445.037
ATTACGG207.0287153E-445.02
TTAAGCG253.887016E-545.01
TAAGCGG502.1827873E-1145.02
GCGTAAG253.887016E-545.01
CGTTTTT17400.042.6724171
TTTAGCG501.0786607E-940.51
TTAGGCG451.9237632E-840.0000041
AAGCGGC451.9237632E-840.00000422
ATCGTTG451.9237632E-840.00000423
AATCGTT451.9237632E-840.00000422
TTAATCG403.451987E-739.37520
GCGGCTA403.451987E-739.37524
CGGGTTC356.239832E-638.5714266
CATGCGG356.239832E-638.5714262
CGTAAGG700.038.5714262