##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551641_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	337967
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8995020223868	33.0	31.0	34.0	30.0	34.0
2	31.97391461296517	33.0	31.0	34.0	30.0	34.0
3	31.970994209493828	33.0	31.0	34.0	30.0	34.0
4	35.6531761976761	37.0	35.0	37.0	33.0	37.0
5	35.675814502599366	37.0	35.0	37.0	35.0	37.0
6	35.54780792207523	37.0	35.0	37.0	33.0	37.0
7	35.83190962431243	37.0	35.0	37.0	35.0	37.0
8	35.48024215381975	37.0	35.0	37.0	33.0	37.0
9	37.274233874904944	39.0	37.0	39.0	34.0	39.0
10	37.082800983527974	39.0	37.0	39.0	34.0	39.0
11	37.16683581533108	39.0	37.0	39.0	34.0	39.0
12	37.03995656380653	39.0	37.0	39.0	33.0	39.0
13	37.02740504250415	39.0	37.0	39.0	33.0	39.0
14	38.05253471492779	40.0	37.0	41.0	33.0	41.0
15	38.072362094524024	40.0	37.0	41.0	33.0	41.0
16	37.9596321534351	40.0	37.0	41.0	33.0	41.0
17	37.87952374048354	40.0	36.0	41.0	33.0	41.0
18	37.73795666440807	39.0	36.0	41.0	33.0	41.0
19	37.58795089461397	39.0	36.0	41.0	33.0	41.0
20	37.71067293552329	39.0	36.0	41.0	33.0	41.0
21	37.692546905467104	39.0	36.0	41.0	33.0	41.0
22	37.81375104669983	40.0	36.0	41.0	33.0	41.0
23	37.79600966958313	40.0	36.0	41.0	33.0	41.0
24	37.67729097811325	40.0	36.0	41.0	33.0	41.0
25	37.389582414851155	39.0	35.0	41.0	33.0	41.0
26	37.54991759550489	39.0	35.0	41.0	33.0	41.0
27	37.53422079670501	39.0	35.0	41.0	33.0	41.0
28	37.4327256803179	39.0	35.0	41.0	33.0	41.0
29	37.44490438415585	40.0	35.0	41.0	33.0	41.0
30	37.2021469551761	39.0	35.0	41.0	32.0	41.0
31	37.220187178038096	39.0	35.0	41.0	32.0	41.0
32	37.07868815594422	39.0	35.0	41.0	32.0	41.0
33	37.02146363402344	39.0	35.0	41.0	32.0	41.0
34	36.84873375211189	39.0	35.0	41.0	31.0	41.0
35	36.74709069228653	39.0	35.0	41.0	31.0	41.0
36	36.60483124091997	39.0	35.0	41.0	31.0	41.0
37	36.4814316190634	39.0	35.0	41.0	31.0	41.0
38	36.41528610781526	39.0	35.0	41.0	30.0	41.0
39	36.34980634203931	39.0	35.0	41.0	30.0	41.0
40	36.184559439235194	39.0	35.0	41.0	30.0	41.0
41	36.07174960868961	39.0	35.0	40.0	30.0	41.0
42	36.02536046418733	39.0	35.0	40.0	30.0	41.0
43	36.00495906405063	39.0	35.0	40.0	30.0	41.0
44	36.07852541816213	39.0	35.0	40.0	30.0	41.0
45	36.09766338133605	39.0	35.0	40.0	30.0	41.0
46	36.01391851867194	38.0	35.0	40.0	30.0	41.0
47	35.857033378998544	38.0	35.0	40.0	30.0	41.0
48	35.8291756295733	38.0	35.0	40.0	30.0	41.0
49	35.81876337038823	38.0	35.0	40.0	30.0	41.0
50	35.65133282243533	38.0	35.0	40.0	29.0	41.0
51	34.79912535839298	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	12.0
10	10.0
11	7.0
12	12.0
13	15.0
14	19.0
15	21.0
16	26.0
17	68.0
18	115.0
19	247.0
20	546.0
21	954.0
22	1463.0
23	1745.0
24	2135.0
25	2550.0
26	3101.0
27	3637.0
28	3743.0
29	4188.0
30	5065.0
31	6295.0
32	8352.0
33	11878.0
34	21695.0
35	28033.0
36	27656.0
37	41830.0
38	71799.0
39	90710.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.19573212769294	18.196155245926377	21.754786709945055	12.85332591643563
2	29.604073770516052	31.775291670488535	22.306023960919262	16.314610598076143
3	29.578331612258	29.862678900602724	25.174351341994928	15.38463814514435
4	27.824018321315396	22.63800903638521	32.37653380359621	17.161438838703187
5	30.030742646471403	26.751724280772976	22.645110321421914	20.57242275133371
6	24.513340059828327	37.83594256243953	23.193980477383885	14.456736900348258
7	70.78620101962618	6.375178641701705	17.451112090825436	5.387508247846683
8	68.94164223134212	12.601526184509137	11.6449239126897	6.811907671459048
9	63.6363905351706	8.097240263102611	13.067548015042888	15.198821186683906
10	41.20254344359064	21.81662706713969	19.608127420724507	17.372702068545152
11	33.357102912414526	21.28965254004089	24.571038000751553	20.78220654679303
12	25.515804797509816	19.97088473134951	30.500019232646974	24.0132912384937
13	24.676373728796005	22.622622918805682	34.24002935197815	18.460974000420162
14	20.69787878698216	28.273470486763504	28.611077412883507	22.41757331337083
15	18.25503673435572	22.380291566928133	37.105101977412	22.25956972130415
16	20.41501093302009	22.718786153677726	29.101361967292664	27.76484094600952
17	20.46116928575867	24.570742113875024	29.666210014587225	25.301878585779086
18	21.09850961780292	23.237475848233704	30.29644906159477	25.367565472368604
19	22.648365077063737	24.709513058967296	26.897004737148894	25.745117126820073
20	24.499729263507973	24.864557782268683	30.033997402113222	20.601715552110118
21	23.059647835439552	28.976497705397275	27.473392372628098	20.49046208653508
22	21.400018344986343	22.5069311500827	28.982119556051327	27.110930948879624
23	22.403962517050484	26.671243050356985	27.726375652060703	23.198418780531828
24	23.856471193933135	24.873138501688036	26.46145925489767	24.80893104948116
25	20.61473457467741	30.03310974148364	25.404255445058244	23.947900238780708
26	20.91269265934248	24.74058118100288	28.445084875150535	25.901641284504105
27	25.18855391206834	26.25581787570976	25.166954170081695	23.38867404214021
28	20.640180846058932	25.300399151396434	29.416481490796436	24.642938511748188
29	25.012797107409895	24.52073723174156	26.509688815771952	23.95677684507659
30	22.356620616805785	29.15491749194448	26.62774767950717	21.860714211742565
31	26.264990368882167	24.80182976444446	21.77372347004293	27.15945639663044
32	25.919098610219343	29.808531602197846	22.700145280456375	21.572224507126435
33	24.32959430950359	24.831418452097395	24.307698680640417	26.531288557758597
34	26.905585456568243	24.5452958424935	24.007373500963112	24.541745199975146
35	24.70803362458465	27.568372059994022	23.85824651519231	23.865347800229017
36	27.69116511375371	25.898090641985753	23.401101290954443	23.00964295330609
37	22.49894220441641	29.464119277917668	26.05579834717591	21.98114017049002
38	21.54677823574491	27.487299055824977	24.350602277737178	26.615320430692936
39	27.20117644622108	25.699846434711084	25.105705586640113	21.993271532427723
40	23.06526968609361	25.466391689129413	28.91465734820263	22.553681276574338
41	25.559300168359634	24.93971304890714	23.409090236620735	26.09189654611249
42	23.31440643613134	25.872348483727702	27.296156133587008	23.517088946553955
43	27.008849976476995	23.04366994410697	24.961608677770315	24.985871401645724
44	23.516793059677426	23.213804898111352	28.010131166652364	25.259270875558858
45	23.23925116949288	23.030946808416207	26.05076827027491	27.679033751816007
46	27.450904970011862	25.610784484875744	25.449822024043765	21.488488521068625
47	21.310660508274477	24.150286862326794	32.14840502179207	22.39064760760666
48	23.875112067154486	24.50742232229774	26.169714794639713	25.44775081590806
49	22.77174990457648	21.697384655898357	31.71256365266431	23.818301786860847
50	23.75794086404886	21.604476176668136	29.01466711246956	25.62291584681345
51	23.512354756529483	22.06073374027642	25.621732299307332	28.805179203886773
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	156.0
1	371.0
2	586.0
3	1729.0
4	2872.0
5	1934.0
6	996.0
7	1020.0
8	1044.0
9	1024.5
10	1005.0
11	979.0
12	953.0
13	967.0
14	981.0
15	922.0
16	863.0
17	798.5
18	734.0
19	734.5
20	735.0
21	775.5
22	816.0
23	836.5
24	857.0
25	1136.0
26	1899.5
27	2384.0
28	2635.5
29	2887.0
30	3193.5
31	3500.0
32	3825.5
33	4151.0
34	4671.0
35	5191.0
36	5645.0
37	6099.0
38	6514.0
39	6929.0
40	8827.5
41	10726.0
42	12452.0
43	14178.0
44	16436.5
45	18695.0
46	32958.0
47	47221.0
48	40731.0
49	34241.0
50	33576.5
51	32912.0
52	28571.0
53	24230.0
54	22008.0
55	19786.0
56	18599.5
57	17413.0
58	16280.0
59	15147.0
60	14141.5
61	13136.0
62	12575.0
63	12014.0
64	11151.5
65	10289.0
66	9058.5
67	7828.0
68	6519.5
69	5211.0
70	4594.5
71	3978.0
72	3267.0
73	2556.0
74	2047.0
75	1152.5
76	767.0
77	617.0
78	467.0
79	384.5
80	302.0
81	212.0
82	122.0
83	75.0
84	28.0
85	22.5
86	17.0
87	9.0
88	1.0
89	2.0
90	3.0
91	2.5
92	2.0
93	3.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	337967.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.48533594157783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.37054330350696	22.062403248819155
2	9.778921264632059	5.962273997970948
3	3.43758095489295	3.1438743070884443
4	1.921746515615562	2.343403524923882
5	1.324243183714603	2.018499916194212
6	1.0226252426840339	1.8705044439336196
7	0.8818127523986974	1.8817650596109177
8	0.7060492308351475	1.721931839464168
9	0.691069384274158	1.896073410468334
>10	7.710616615146295	43.583506159357
>50	0.09087762102747604	1.914644450403263
>100	0.050931413982431624	2.9725852144597646
>500	0.006990586232882773	1.744156154612863
>1k	0.002995965528378331	1.7627272011186028
>5k	0.002995965528378331	5.121651071574828
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	5802	1.7167356576233774	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	5798	1.7155521101172597	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	5223	1.545417156112875	No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	2674	0.7912015078395227	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1834	0.5426565315548559	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT	1282	0.37932697571064633	No Hit
GAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT	959	0.2837555145916613	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTA	935	0.27665422955495655	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATGAGT	905	0.2677776232590756	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC	844	0.24972852379078428	No Hit
CCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	775	0.2293123293102581	No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT	672	0.19883598102773348	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	639	0.18907171410226442	No Hit
TCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	488	0.14439279574633027	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	454	0.13433264194433184	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCC	432	0.12782313066068582	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	427	0.12634369627803899	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20652903981749698	0.0
2	0.0	0.0	0.0	1.5099107309293511	0.0
3	0.0	0.0	0.0	1.8984101998124077	0.0
4	0.0	0.0	0.0	2.7064772596141045	0.0
5	0.0	0.0	0.0	5.611494613379413	0.0
6	0.0	0.0	0.0	6.554781975755029	0.0
7	0.0	0.0	0.0	7.7504608438101945	0.0
8	0.0	0.0	0.0	9.284930185491483	0.0
9	0.0	0.0	0.0	9.871377974772685	0.0
10	0.0	0.0	0.0	12.69443466373936	0.0
11	0.0	0.0	0.0	14.0510759926265	0.0
12	0.0	0.0	0.0	16.89839540546858	0.0
13	0.0	0.0	0.0	17.48661851600896	0.0
14	0.0	0.0	0.0	17.749366062367034	0.0
15	0.0	0.0	0.0	18.378421561868468	0.0
16	0.0	0.0	0.0	19.128494793870406	0.0
17	0.0	0.0	0.0	19.921767509845637	0.0
18	0.0	0.0	0.0	20.834874410815257	0.0
19	0.0	0.0	0.0	21.608322706063017	0.0
20	0.0	0.0	0.0	22.14802036885258	0.0
21	0.0	0.0	0.0	22.660200552124913	0.0
22	0.0	0.0	0.0	23.209366594963413	0.0
23	0.0	0.0	0.0	23.76622569659168	0.0
24	0.0	0.0	0.0	24.195261667559258	0.0
25	0.0	0.0	0.0	24.572813322010727	0.0
26	0.0	0.0	0.0	24.947406107696906	0.0
27	0.0	0.0	0.0	25.363423056097194	0.0
28	0.0	0.0	0.0	25.759911470646543	0.0
29	0.0	0.0	0.0	26.113200401222606	0.0
30	0.0	0.0	0.0	26.532176218388187	0.0
31	0.0	0.0	0.0	26.92156334790083	0.0
32	0.0	0.0	0.0	27.277811147242186	0.0
33	0.0	0.0	0.0	27.664535294866067	0.0
34	0.0	0.0	0.0	28.011018827281955	0.0
35	0.0	0.0	0.0	28.37200081664778	0.0
36	0.0	0.0	0.0	28.72528974722384	0.0
37	0.0	0.0	0.0	29.07946633842949	0.0
38	0.0	0.0	0.0	29.41233907452503	0.0
39	0.0	0.0	0.0	29.77036219512556	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGGC	30	2.1609376E-6	45.000004	17
TGATTCG	30	2.1609376E-6	45.000004	15
ACTAAGG	30	2.1609376E-6	45.000004	2
TAGATAG	30	2.1609376E-6	45.000004	1
TTCGGCA	30	2.1609376E-6	45.000004	18
GATTCGG	30	2.1609376E-6	45.000004	16
GGTAATC	20	7.0262124E-4	45.0	8
CTACTCG	20	7.0262124E-4	45.0	36
CCTACGC	20	7.0262124E-4	45.0	38
GTGATAG	20	7.0262124E-4	45.0	1
ATCACAC	20	7.0262124E-4	45.0	12
TACGAAT	95	0.0	45.0	12
GTACTAG	20	7.0262124E-4	45.0	1
CAGTCGA	20	7.0262124E-4	45.0	41
GCTACGA	95	0.0	45.0	10
CAGTACG	20	7.0262124E-4	45.0	2
AGCTACG	95	0.0	45.0	9
GATAGCG	45	3.8380676E-10	45.0	1
ACGCGTG	20	7.0262124E-4	45.0	41
TCAAAGT	20	7.0262124E-4	45.0	12
>>END_MODULE