FastQCFastQC Report
Sat 18 Jun 2016
SRR3551636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573464
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71621.2489014131663017No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC43270.7545373380020368No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCG42060.7334374956405284No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGC33480.5838204316225605No Hit
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC18250.3182414240475427No Hit
GAACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT11580.20193072276550925No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCT7990.13932871113095155No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAACGACGT7310.12747094848150886No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA6170.10759175815744318No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCG253.8883525E-545.029
AAGTCGA207.030327E-445.023
CTCACGT302.1635915E-644.99999645
CGTTTTT31150.042.544141
CGATGAA6150.040.60975619
GTACGAG501.0786607E-940.51
TACGGCT4150.040.1204837
CGTAAGG451.9254003E-840.0000042
CGTATGG403.454188E-739.3752
CGTTCGG403.454188E-739.37545
CGATACG403.454188E-739.37510
GTCGAAT750.039.00000443
CCGATGA6150.038.78048718
GATGAAT6650.038.5714320
ATAGCGG700.038.5714262
CGAATAT700.038.57142614
CGTGTAC700.038.57142618
CGGTCTA356.2428153E-638.57142631
ACGGTCT356.2428153E-638.57142630
TACGGGA1650.038.181824