##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551636_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	573464
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.058338448446634	33.0	31.0	34.0	30.0	34.0
2	32.170612279061984	33.0	31.0	34.0	30.0	34.0
3	32.13827023143563	33.0	31.0	34.0	30.0	34.0
4	35.78940439155727	37.0	35.0	37.0	35.0	37.0
5	35.79276467223749	37.0	35.0	37.0	35.0	37.0
6	35.675775637180365	37.0	35.0	37.0	35.0	37.0
7	35.93373080088724	37.0	35.0	37.0	35.0	37.0
8	35.69064143520779	37.0	35.0	37.0	35.0	37.0
9	37.51981815772219	39.0	37.0	39.0	35.0	39.0
10	37.26459027942469	39.0	37.0	39.0	34.0	39.0
11	37.32605010950993	39.0	37.0	39.0	34.0	39.0
12	37.25826555808211	39.0	37.0	39.0	34.0	39.0
13	37.32263577138234	39.0	37.0	39.0	34.0	39.0
14	38.47580842040651	40.0	38.0	41.0	34.0	41.0
15	38.50425833182205	40.0	38.0	41.0	34.0	41.0
16	38.30580123599738	40.0	38.0	41.0	34.0	41.0
17	38.289303251817024	40.0	38.0	41.0	34.0	41.0
18	38.06625350501513	40.0	37.0	41.0	33.0	41.0
19	37.84016084706276	39.0	37.0	41.0	33.0	41.0
20	37.954011062595036	40.0	36.0	41.0	34.0	41.0
21	37.887039116666436	40.0	36.0	41.0	34.0	41.0
22	38.01644043915573	40.0	36.0	41.0	34.0	41.0
23	38.01823305386214	40.0	36.0	41.0	34.0	41.0
24	37.8952035350083	40.0	36.0	41.0	33.0	41.0
25	37.5755932368902	39.0	35.0	41.0	33.0	41.0
26	37.7004816344182	40.0	36.0	41.0	33.0	41.0
27	37.75879566982409	40.0	36.0	41.0	33.0	41.0
28	37.63027496059038	40.0	35.0	41.0	33.0	41.0
29	37.63451585452618	40.0	35.0	41.0	33.0	41.0
30	37.37484131523513	40.0	35.0	41.0	33.0	41.0
31	37.231997126236344	39.0	35.0	41.0	33.0	41.0
32	37.11104097205753	40.0	35.0	41.0	32.0	41.0
33	36.93535078051979	40.0	35.0	41.0	31.0	41.0
34	36.682836237322654	40.0	35.0	41.0	31.0	41.0
35	36.57054322503244	40.0	35.0	41.0	31.0	41.0
36	36.40451885384261	39.0	35.0	41.0	30.0	41.0
37	36.352601035112926	39.0	35.0	41.0	30.0	41.0
38	36.172866997754	39.0	35.0	41.0	30.0	41.0
39	36.05975789238732	39.0	35.0	41.0	29.0	41.0
40	35.97677622309334	39.0	35.0	41.0	29.0	41.0
41	35.77303544773517	39.0	35.0	41.0	28.0	41.0
42	35.80133539332896	39.0	35.0	41.0	28.0	41.0
43	35.76182811824282	39.0	35.0	41.0	28.0	41.0
44	35.821207608498526	39.0	35.0	41.0	28.0	41.0
45	35.785168728987344	39.0	35.0	41.0	28.0	41.0
46	35.67298906295775	38.0	35.0	41.0	28.0	41.0
47	35.5929840408465	38.0	35.0	41.0	28.0	41.0
48	35.55840471241438	38.0	35.0	40.0	28.0	41.0
49	35.50769359541314	38.0	35.0	40.0	28.0	41.0
50	35.34442266646206	38.0	35.0	40.0	27.0	41.0
51	34.487240698631474	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	8.0
9	15.0
10	19.0
11	24.0
12	17.0
13	31.0
14	45.0
15	47.0
16	94.0
17	128.0
18	199.0
19	394.0
20	797.0
21	1411.0
22	1949.0
23	2532.0
24	3541.0
25	5016.0
26	7076.0
27	8402.0
28	8685.0
29	8137.0
30	8523.0
31	10308.0
32	12634.0
33	17552.0
34	31096.0
35	41114.0
36	45774.0
37	68790.0
38	117669.0
39	171340.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16469734804626	17.962243488693275	21.68174462564346	17.191314537617007
2	33.15290933694182	27.20589261052132	23.579684165004256	16.06151388753261
3	30.793040190840227	25.268194690512395	28.327148696343624	15.611616422303754
4	27.368936846951158	23.41576803426196	31.516538091318726	17.698757027468158
5	27.444094136685127	27.522739003668935	25.856025835972268	19.177141023673673
6	25.71826653460374	34.646289915321624	25.359394835595612	14.276048714479025
7	73.30747876065455	6.52020004743105	15.594352914917064	4.5779682769973356
8	73.53207873554399	9.049216690149686	12.483434008063279	4.935270566243042
9	67.05355523624849	8.389367074480699	14.481641393356862	10.075436295913955
10	39.48617524378165	24.06149296206911	20.49806090704909	15.954270887100149
11	30.918767350696818	22.928030355872384	26.73506968179345	19.418132611637347
12	26.246285730228923	20.537121772247254	31.0807653139517	22.135827183572115
13	24.764065399048587	20.856235090607257	33.76445600770057	20.615243502643583
14	20.74620202837493	26.161886360782894	30.85790912768718	22.234002483155002
15	20.136922282828564	22.67465786867179	35.5021413724314	21.686278476068242
16	22.585201512213494	22.185699538244773	31.768341168756887	23.460757780784842
17	22.23278183111756	23.592065064241172	30.74595789796744	23.42919520667383
18	23.24348171812006	22.77963394389186	30.34575840854875	23.631125929439335
19	24.665715720603213	23.546203423405828	28.37771856646625	23.410362289524713
20	26.203388530055943	23.46982548163442	29.888362652232747	20.438423336076895
21	24.558647099033244	25.902410613395087	28.46787243837451	21.071069849197162
22	23.272079851568712	22.932564206297172	29.609879608833335	24.18547633330078
23	24.786560272310034	24.328467000544062	28.89928574417923	21.985686982966673
24	23.55213230473055	23.747959767308846	29.170270496491497	23.52963743146911
25	22.82723937335212	26.70873847355719	27.923287250812606	22.540734902278086
26	22.4872005914931	24.693093202014424	29.53681486544927	23.282891341043204
27	25.118228868769442	24.225060335086425	28.070986147343163	22.58572464880097
28	22.00591493101572	26.55389004366447	29.203751238089925	22.236443787229888
29	23.38420546015094	25.12363461350669	28.232635352873064	23.259524573469303
30	22.90152476877363	27.6020813861027	27.25280052453162	22.24359332059205
31	23.81317746188078	26.694090649107878	25.256336927862954	24.236394961148388
32	25.482506312514825	26.826967342326636	25.306209282535608	22.384317062622937
33	25.15851038600505	26.52616380452827	24.383221963366488	23.93210384610019
34	22.99516621793173	28.238738613060278	25.29958288576092	23.466512283247074
35	24.218085180586748	26.745358034680468	24.513134215922882	24.5234225688099
36	23.757201847020912	30.025075680426323	23.90089002971416	22.316832442838606
37	23.419778748099272	28.1532929704393	25.281970620649247	23.14495766081219
38	24.258541076684846	26.967167947770044	24.395602862603408	24.378688112941703
39	25.018832917149116	26.171651577082432	25.291735836948785	23.517779668819664
40	23.897751210189305	25.154150914442752	27.71246320606001	23.235634669307924
41	22.737085501443858	26.849287836725583	26.03877488386368	24.37485177796688
42	22.586247785388448	26.102423168673184	27.174155657547814	24.137173388390554
43	24.613576440718163	25.013950308999345	26.63515059358565	23.737322656696847
44	24.0170263521337	25.00871894312459	27.11033299387581	23.863921710865895
45	22.82706499448963	24.060272310031667	27.320285144315946	25.792377551162758
46	23.96785151291101	26.055340875800397	26.24959892861627	23.72720868267232
47	22.376121256085824	25.465417183990624	29.415795934879956	22.742665625043596
48	23.024113109105368	25.168449981167086	27.378876442113192	24.428560467614357
49	22.967091221070547	24.082069667843143	29.17829192416612	23.77254718692019
50	22.49452450371776	24.110144664704322	28.744611693149004	24.65071913842892
51	22.492083199642874	24.51383173137285	27.525529065468803	25.46855600351548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	210.0
1	563.5
2	917.0
3	6131.0
4	11345.0
5	7487.0
6	3629.0
7	3527.0
8	3425.0
9	3367.5
10	3310.0
11	3207.0
12	3104.0
13	2861.5
14	2619.0
15	2515.0
16	2411.0
17	2296.5
18	2182.0
19	2040.5
20	1899.0
21	1897.5
22	1896.0
23	1967.0
24	2038.0
25	2441.0
26	3426.0
27	4008.0
28	4817.5
29	5627.0
30	6695.0
31	7763.0
32	8455.5
33	9148.0
34	10102.0
35	11056.0
36	11660.5
37	12265.0
38	13101.5
39	13938.0
40	16552.0
41	19166.0
42	21193.5
43	23221.0
44	26286.5
45	29352.0
46	40843.5
47	52335.0
48	49398.0
49	46461.0
50	45825.5
51	45190.0
52	41189.5
53	37189.0
54	35103.5
55	33018.0
56	31496.0
57	29974.0
58	29519.5
59	29065.0
60	29280.0
61	29495.0
62	27687.5
63	25880.0
64	23148.0
65	20416.0
66	17281.0
67	14146.0
68	12381.0
69	10616.0
70	9218.0
71	7820.0
72	6825.0
73	5830.0
74	4824.0
75	3025.5
76	2233.0
77	1791.0
78	1349.0
79	1019.5
80	690.0
81	495.0
82	300.0
83	227.0
84	154.0
85	119.0
86	84.0
87	51.5
88	19.0
89	12.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	573464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.02609166497126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.10727168382456	24.1950254435983
2	7.600929248867853	5.172598307218776
3	3.618138498486309	3.6933333661816987
4	2.4623981129330677	3.351431356252512
5	2.0559746831948056	3.4978391515623355
6	1.7931718691571925	3.660877823459429
7	1.57589531400042	3.753509088600387
8	1.3725552236680338	3.736215188461092
9	1.2275924153803253	3.759315484765898
>10	7.089246064707629	36.95814954290547
>50	0.05768410216682085	1.3472976646919104
>100	0.03244730746873108	2.2487673820608647
>500	0.0036052563854145647	0.7656534205286719
>1k	0.0025751831324389747	2.6048689500430715
>5k	5.15036626487795E-4	1.2551178296695693
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7162	1.2489014131663017	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC	4327	0.7545373380020368	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCG	4206	0.7334374956405284	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGC	3348	0.5838204316225605	No Hit
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	1825	0.3182414240475427	No Hit
GAACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT	1158	0.20193072276550925	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCT	799	0.13932871113095155	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAACGACGT	731	0.12747094848150886	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	617	0.10759175815744318	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.487577249836084E-4	0.0	0.0	0.10288352887016447	0.0
2	3.487577249836084E-4	0.0	0.0	0.5709163957981669	0.0
3	3.487577249836084E-4	0.0	0.0	0.7854023966630861	0.0
4	3.487577249836084E-4	0.0	0.0	1.2044348032308916	0.0
5	3.487577249836084E-4	0.0	0.0	2.534422387455882	0.0
6	3.487577249836084E-4	0.0	0.0	3.327846211793591	0.0
7	3.487577249836084E-4	0.0	0.0	4.025012904035824	0.0
8	3.487577249836084E-4	0.0	0.0	5.020193072276551	0.0
9	3.487577249836084E-4	0.0	0.0	5.535831368664816	0.0
10	3.487577249836084E-4	0.0	0.0	7.046998591018791	0.0
11	3.487577249836084E-4	0.0	0.0	7.855942134118271	0.0
12	3.487577249836084E-4	0.0	0.0	9.122455812396245	0.0
13	3.487577249836084E-4	0.0	0.0	9.469295369892443	0.0
14	3.487577249836084E-4	0.0	0.0	9.65553199503369	0.0
15	3.487577249836084E-4	0.0	0.0	9.9531967133072	0.0
16	3.487577249836084E-4	0.0	0.0	10.344851638463792	0.0
17	3.487577249836084E-4	0.0	0.0	10.74766681081986	0.0
18	3.487577249836084E-4	0.0	0.0	11.229475607884714	0.0
19	3.487577249836084E-4	0.0	0.0	11.630895749340848	0.0
20	3.487577249836084E-4	0.0	0.0	11.919144009039801	0.0
21	3.487577249836084E-4	0.0	0.0	12.196406400401768	0.0
22	3.487577249836084E-4	0.0	0.0	12.51342717241187	0.0
23	3.487577249836084E-4	0.0	0.0	12.816323256560132	0.0
24	3.487577249836084E-4	0.0	0.0	13.071439532385643	0.0
25	5.231365874754126E-4	0.0	0.0	13.313477393524266	0.0
26	5.231365874754126E-4	0.0	0.0	13.521685755339481	0.0
27	5.231365874754126E-4	0.0	0.0	13.764944268515547	0.0
28	5.231365874754126E-4	0.0	0.0	13.993031820654828	0.0
29	5.231365874754126E-4	0.0	0.0	14.212400429669517	0.0
30	5.231365874754126E-4	0.0	0.0	14.496114838943683	0.0
31	6.975154499672168E-4	0.0	0.0	14.745650991169454	0.0
32	6.975154499672168E-4	0.0	0.0	14.997279689745127	0.0
33	6.975154499672168E-4	0.0	0.0	15.230598607759163	0.0
34	6.975154499672168E-4	0.0	0.0	15.463568768048212	0.0
35	6.975154499672168E-4	0.0	0.0	15.712756162549	0.0
36	8.71894312459021E-4	0.0	0.0	15.967000544062051	0.0
37	8.71894312459021E-4	0.0	0.0	16.22403498737497	0.0
38	8.71894312459021E-4	0.0	0.0	16.475838064813136	0.0
39	8.71894312459021E-4	0.0	0.0	16.71403959097694	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTCG	25	3.8883525E-5	45.0	29
AAGTCGA	20	7.030327E-4	45.0	23
CTCACGT	30	2.1635915E-6	44.999996	45
CGTTTTT	3115	0.0	42.54414	1
CGATGAA	615	0.0	40.609756	19
GTACGAG	50	1.0786607E-9	40.5	1
TACGGCT	415	0.0	40.120483	7
CGTAAGG	45	1.9254003E-8	40.000004	2
CGTATGG	40	3.454188E-7	39.375	2
CGTTCGG	40	3.454188E-7	39.375	45
CGATACG	40	3.454188E-7	39.375	10
GTCGAAT	75	0.0	39.000004	43
CCGATGA	615	0.0	38.780487	18
GATGAAT	665	0.0	38.57143	20
ATAGCGG	70	0.0	38.571426	2
CGAATAT	70	0.0	38.571426	14
CGTGTAC	70	0.0	38.571426	18
CGGTCTA	35	6.2428153E-6	38.571426	31
ACGGTCT	35	6.2428153E-6	38.571426	30
TACGGGA	165	0.0	38.18182	4
>>END_MODULE