FastQCFastQC Report
Sat 18 Jun 2016
SRR3551635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500441
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62191.2427039351292162No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTC26190.5233384155175136No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCG25160.502756568706401No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGC23230.4641905839049958No Hit
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC17280.3452954494136172Illumina PCR Primer Index 6 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT8540.17064948715233164Illumina PCR Primer Index 6 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCT6820.1362798012153281Illumina PCR Primer Index 6 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC6780.1354805061935373No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5610.11210112680615698No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG207.029464E-445.0000041
ACGCATG207.029464E-445.0000041
TAACGCG302.1630349E-645.0000041
TAGCCGT406.8012014E-945.00000444
CTCGGTA207.029464E-445.00000438
TTAAGCG207.029464E-445.0000041
TCCACGA207.029464E-445.00000435
CGCAATC207.029464E-445.00000420
CGACTCG207.029464E-445.00000435
TGCGTAG600.045.0000041
TTGTGCG253.8876373E-545.01
CGTTTTT28600.043.4265751
TGATTCG501.0786607E-940.515
TCACGAC451.9244908E-840.025
ACGGGAC800.039.3750045
CGAATAT403.4530058E-739.37500414
CGTATGG356.24122E-638.571432
AATGCGG700.038.571432
TTAGTCA700.038.5714332
TATAGCG356.24122E-638.571431