##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551635_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	500441
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99348374733485	33.0	31.0	34.0	30.0	34.0
2	32.09290206038274	33.0	31.0	34.0	30.0	34.0
3	32.04907271786284	33.0	31.0	34.0	30.0	34.0
4	35.71943745616366	37.0	35.0	37.0	33.0	37.0
5	35.75866885407071	37.0	35.0	37.0	35.0	37.0
6	35.648286211561405	37.0	35.0	37.0	33.0	37.0
7	35.96092646285976	37.0	35.0	37.0	35.0	37.0
8	35.77296424553544	37.0	35.0	37.0	35.0	37.0
9	37.59128248884484	39.0	37.0	39.0	35.0	39.0
10	37.22979731876485	39.0	37.0	39.0	34.0	39.0
11	37.2913710107685	39.0	37.0	39.0	34.0	39.0
12	37.24651657238316	39.0	37.0	39.0	34.0	39.0
13	37.31387316386947	39.0	37.0	39.0	34.0	39.0
14	38.40358803535282	40.0	38.0	41.0	34.0	41.0
15	38.47060492645487	40.0	38.0	41.0	34.0	41.0
16	38.27172034265777	40.0	38.0	41.0	34.0	41.0
17	38.27005181429979	40.0	37.0	41.0	34.0	41.0
18	38.044680591718105	39.0	37.0	41.0	33.0	41.0
19	37.808003341053194	39.0	37.0	41.0	33.0	41.0
20	38.03389810187414	40.0	36.0	41.0	34.0	41.0
21	37.917450808387	40.0	36.0	41.0	34.0	41.0
22	38.07834489979838	40.0	36.0	41.0	34.0	41.0
23	38.10721743422302	40.0	36.0	41.0	34.0	41.0
24	37.96946493193004	40.0	36.0	41.0	34.0	41.0
25	37.61297135926113	39.0	35.0	41.0	33.0	41.0
26	37.784250291243126	40.0	36.0	41.0	33.0	41.0
27	37.84278066745131	40.0	36.0	41.0	34.0	41.0
28	37.72326008460538	40.0	36.0	41.0	33.0	41.0
29	37.75489817980541	40.0	36.0	41.0	33.0	41.0
30	37.459598634004806	40.0	35.0	41.0	33.0	41.0
31	37.32802468223027	39.0	35.0	41.0	33.0	41.0
32	37.258731798553676	39.0	35.0	41.0	33.0	41.0
33	37.11641332344872	40.0	35.0	41.0	32.0	41.0
34	36.89678903207371	40.0	35.0	41.0	31.0	41.0
35	36.78811488267348	40.0	35.0	41.0	31.0	41.0
36	36.61179040086643	39.0	35.0	41.0	31.0	41.0
37	36.58123934689604	39.0	35.0	41.0	31.0	41.0
38	36.45289854348464	39.0	35.0	41.0	30.0	41.0
39	36.361696983260764	39.0	35.0	41.0	30.0	41.0
40	36.28173750751837	39.0	35.0	41.0	30.0	41.0
41	36.08479521062423	39.0	35.0	41.0	29.0	41.0
42	36.16488657004522	39.0	35.0	41.0	30.0	41.0
43	36.119734394264256	39.0	35.0	41.0	30.0	41.0
44	36.15443578763531	39.0	35.0	41.0	30.0	41.0
45	36.111957253702236	39.0	35.0	41.0	30.0	41.0
46	35.993713544653616	39.0	35.0	41.0	30.0	41.0
47	35.904616128574595	38.0	35.0	41.0	29.0	41.0
48	35.8552516680288	38.0	35.0	40.0	29.0	41.0
49	35.81971700959753	38.0	35.0	40.0	30.0	41.0
50	35.64311477277042	38.0	35.0	40.0	29.0	41.0
51	34.74015518312848	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	17.0
10	13.0
11	13.0
12	16.0
13	23.0
14	26.0
15	22.0
16	51.0
17	73.0
18	146.0
19	254.0
20	516.0
21	803.0
22	1241.0
23	1833.0
24	2530.0
25	3940.0
26	5375.0
27	6287.0
28	6625.0
29	6723.0
30	7202.0
31	8762.0
32	11278.0
33	15665.0
34	28264.0
35	37762.0
36	41320.0
37	60190.0
38	106543.0
39	146856.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.12844271352667	19.087764591630183	23.18255298826435	15.601239706578799
2	32.935151196644554	25.55186325660767	24.925815430790045	16.587170115957726
3	30.255514636090968	24.798128051058967	29.460016265653692	15.486341047196373
4	27.11868132307305	24.187666478166257	31.068597497007637	17.625054701753054
5	26.04682669885161	28.085628475684448	26.95302742980691	18.91451739565703
6	25.304681271118874	34.10651805107895	26.44947156607872	14.139329111723459
7	75.22784903714923	5.829058770164715	14.165506023687108	4.777586168998943
8	76.03553665666882	6.5668080752776055	11.84795010800474	5.549705160048837
9	69.49250760828949	7.244210606245291	14.25103059101872	9.012251194446499
10	37.67297243830941	25.340449723344012	20.914953011443906	16.07162482690267
11	29.497982779188753	23.54423398562468	27.99330990066761	18.964473334518956
12	25.729506575200674	20.433577584570408	31.85050785207447	21.986407988154447
13	24.293772892308986	21.555787795164665	34.26198093281725	19.888458379709096
14	21.178120897368522	25.742495119304778	31.18989051656439	21.889493466762318
15	19.15270731215068	23.095629654644604	36.4428573997734	21.308805633431312
16	21.526413703113853	22.5756882429697	33.667505260360365	22.230392793556085
17	21.800372071832644	23.593990100731155	31.418688716552	23.1869491108842
18	21.4181092276612	23.641548154527705	32.15763696419758	22.782705653613515
19	22.650821975018033	25.592227655208106	29.21343375143124	22.543516618342622
20	24.701813000933175	24.94939463393287	29.15428591981872	21.19450644531523
21	23.012103324867468	27.17063549948945	28.790606684903914	21.02665449073917
22	21.870510209994784	24.35651755151956	28.592980990766144	25.179991247719514
23	21.83354281523696	26.67846958982178	28.54202593312698	22.94596166181428
24	22.137474747272908	24.58371716146359	29.763948197689636	23.514859893573867
25	21.96542649383244	26.986397996966677	27.9467509656483	23.101424543552586
26	21.65849720546478	25.754084897120737	29.42664569849393	23.16077219892055
27	23.973855059837224	25.5536616704067	28.425728507456423	22.046754762299653
28	21.862517259776876	25.530082467263878	29.94139169252719	22.66600858043206
29	24.20385220235752	23.922500354687166	28.227103694541416	23.646543748413897
30	24.404675076582453	25.249130267104412	28.202325548865904	22.14386910744723
31	24.48040827989713	25.72111397747187	24.962782825547865	24.83569491708313
32	26.317188239972346	26.84052665548986	24.79513069472725	22.047154409810545
33	24.585915222773515	25.430969884561815	25.8440055870722	24.13910930559247
34	23.950875327960738	26.184705090110523	26.278422431415493	23.585997150513247
35	23.813196760457277	26.851716785794927	26.15113469919531	23.183951754552485
36	24.00083126682266	26.89248083190626	25.794449295721172	23.312238605549904
37	22.100107705004184	29.003618808211158	27.25496112428838	21.64131236249628
38	20.52449739329911	28.348996185364513	26.97900451801511	24.14750190332127
39	21.49024560337782	27.93955731045218	26.93903976692557	23.631157319244426
40	22.58188277938858	26.451869451144088	29.05277545205129	21.913472317416037
41	20.82123567013894	26.734819888858024	26.83952753671262	25.604416904290417
42	22.28454503128241	26.616124578122097	27.272545614767772	23.82678477582772
43	23.07065168521364	24.73898021944645	27.621038244268554	24.569329851071355
44	23.232708750881724	25.015336473230608	27.629830489508254	24.122124286379414
45	22.40503875581737	24.225433167945873	28.40294859933539	24.966579476901373
46	23.74245915102879	26.08119638478862	27.39004198297102	22.78630248121157
47	21.710251558125734	25.713720498520303	29.539546120321873	23.036481823032084
48	21.865314792353143	24.761160656301143	28.2195103918344	25.154014159511313
49	22.120689551815296	23.68291167190538	29.728579392975398	24.467819383303926
50	21.815958324757563	23.383575686244733	29.8107069564644	24.989759032533307
51	21.351168269586225	23.708489112602685	28.052857379791025	26.887485238020066
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	413.0
2	660.0
3	4754.0
4	8848.0
5	5698.5
6	2549.0
7	2501.5
8	2454.0
9	2485.5
10	2517.0
11	2554.5
12	2592.0
13	2570.0
14	2548.0
15	2467.0
16	2386.0
17	2236.0
18	2086.0
19	2107.0
20	2128.0
21	2031.5
22	1935.0
23	2022.0
24	2109.0
25	2294.0
26	3037.0
27	3595.0
28	4442.0
29	5289.0
30	5817.5
31	6346.0
32	7116.0
33	7886.0
34	8766.5
35	9647.0
36	10746.0
37	11845.0
38	12682.0
39	13519.0
40	15475.5
41	17432.0
42	20037.5
43	22643.0
44	25905.5
45	29168.0
46	38836.0
47	48504.0
48	48576.5
49	48649.0
50	47351.5
51	46054.0
52	40499.0
53	34944.0
54	31221.0
55	27498.0
56	26179.0
57	24860.0
58	23628.5
59	22397.0
60	20874.5
61	19352.0
62	18325.0
63	17298.0
64	15202.5
65	13107.0
66	11522.0
67	9937.0
68	8783.5
69	7630.0
70	6881.5
71	6133.0
72	5190.5
73	4248.0
74	3444.5
75	2206.5
76	1772.0
77	1461.5
78	1151.0
79	946.5
80	742.0
81	580.5
82	419.0
83	277.5
84	136.0
85	113.0
86	90.0
87	56.5
88	23.0
89	25.5
90	28.0
91	14.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	500441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.417777271250735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28553313278645	26.31028466973147
2	8.64784308344805	6.125747604125785
3	3.307676995934389	3.514517013817317
4	1.9537047074095302	2.767835127232993
5	1.401564121095599	2.4820142936170106
6	1.119874776013362	2.3798085233119832
7	0.9691940356009187	2.4028688939876592
8	0.7884729553109905	2.2340767612487595
9	0.7449013079337623	2.3744473752115214
>10	6.684218995846577	42.118871492460855
>50	0.06127319281288726	1.4771155864836356
>100	0.03063659640644356	2.1593094942665108
>500	0.0022693775115884118	0.5576106315456513
>1k	0.0022693775115884118	1.845841895992199
>5k	5.673443778971029E-4	1.2496506369666323
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6219	1.2427039351292162	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTC	2619	0.5233384155175136	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCG	2516	0.502756568706401	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGC	2323	0.4641905839049958	No Hit
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	1728	0.3452954494136172	Illumina PCR Primer Index 6 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT	854	0.17064948715233164	Illumina PCR Primer Index 6 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCT	682	0.1362798012153281	Illumina PCR Primer Index 6 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC	678	0.1354805061935373	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	561	0.11210112680615698	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19083168645254886	0.0
2	0.0	0.0	0.0	0.8268707000425625	0.0
3	0.0	0.0	0.0	1.165372141770958	0.0
4	0.0	0.0	0.0	1.7250784807799522	0.0
5	0.0	0.0	0.0	3.334858654666584	0.0
6	0.0	0.0	0.0	4.460266045348003	0.0
7	0.0	0.0	0.0	5.52172983428616	0.0
8	0.0	0.0	0.0	7.352315257942495	0.0
9	0.0	0.0	0.0	8.314666464178595	0.0
10	0.0	0.0	0.0	10.285328340403764	0.0
11	0.0	0.0	0.0	11.695084935087253	0.0
12	0.0	0.0	0.0	13.297271806266872	0.0
13	0.0	0.0	0.0	13.837195593486545	0.0
14	0.0	0.0	0.0	14.085376697752583	0.0
15	0.0	0.0	0.0	14.452852584020894	0.0
16	0.0	0.0	0.0	15.029144294732045	0.0
17	0.0	0.0	0.0	15.714339952162193	0.0
18	0.0	0.0	0.0	16.491454537098278	0.0
19	0.0	0.0	0.0	17.02618290667631	0.0
20	0.0	0.0	0.0	17.45980045599781	0.0
21	1.9982375544769514E-4	0.0	0.0	17.939777116583173	0.0
22	1.9982375544769514E-4	0.0	0.0	18.441134919001442	0.0
23	3.996475108953903E-4	0.0	0.0	18.941293778887022	0.0
24	3.996475108953903E-4	0.0	0.0	19.364920140436137	0.0
25	3.996475108953903E-4	0.0	0.0	19.727000785307357	0.0
26	3.996475108953903E-4	0.0	0.0	20.081887774982466	0.0
27	3.996475108953903E-4	0.0	0.0	20.44756524745175	0.0
28	3.996475108953903E-4	0.0	0.0	20.811444306122	0.0
29	3.996475108953903E-4	0.0	0.0	21.163333939465392	0.0
30	3.996475108953903E-4	0.0	0.0	21.5701751055569	0.0
31	5.994712663430854E-4	0.0	0.0	21.9296580416073	0.0
32	5.994712663430854E-4	0.0	0.0	22.28794203512502	0.0
33	5.994712663430854E-4	0.0	0.0	22.626643300608862	0.0
34	5.994712663430854E-4	0.0	0.0	22.975735401375985	0.0
35	7.992950217907805E-4	0.0	0.0	23.369588023363395	0.0
36	7.992950217907805E-4	0.0	0.0	23.742459151028793	0.0
37	7.992950217907805E-4	0.0	0.0	24.095947374415765	0.0
38	7.992950217907805E-4	0.0	0.0	24.446238417715573	0.0
39	7.992950217907805E-4	0.0	0.0	24.79253298590643	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	20	7.029464E-4	45.000004	1
ACGCATG	20	7.029464E-4	45.000004	1
TAACGCG	30	2.1630349E-6	45.000004	1
TAGCCGT	40	6.8012014E-9	45.000004	44
CTCGGTA	20	7.029464E-4	45.000004	38
TTAAGCG	20	7.029464E-4	45.000004	1
TCCACGA	20	7.029464E-4	45.000004	35
CGCAATC	20	7.029464E-4	45.000004	20
CGACTCG	20	7.029464E-4	45.000004	35
TGCGTAG	60	0.0	45.000004	1
TTGTGCG	25	3.8876373E-5	45.0	1
CGTTTTT	2860	0.0	43.426575	1
TGATTCG	50	1.0786607E-9	40.5	15
TCACGAC	45	1.9244908E-8	40.0	25
ACGGGAC	80	0.0	39.375004	5
CGAATAT	40	3.4530058E-7	39.375004	14
CGTATGG	35	6.24122E-6	38.57143	2
AATGCGG	70	0.0	38.57143	2
TTAGTCA	70	0.0	38.57143	32
TATAGCG	35	6.24122E-6	38.57143	1
>>END_MODULE