##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551632_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 339761 Sequences flagged as poor quality 0 Sequence length 51 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.014033394062295 33.0 31.0 34.0 30.0 34.0 2 32.11149013571305 33.0 31.0 34.0 30.0 34.0 3 32.09427509337446 33.0 31.0 34.0 30.0 34.0 4 35.755660596713575 37.0 35.0 37.0 35.0 37.0 5 35.74975055995244 37.0 35.0 37.0 35.0 37.0 6 35.62701428356992 37.0 35.0 37.0 33.0 37.0 7 35.9061163582636 37.0 35.0 37.0 35.0 37.0 8 35.61434066888195 37.0 35.0 37.0 33.0 37.0 9 37.38940019602014 39.0 37.0 39.0 34.0 39.0 10 37.15092668081387 39.0 37.0 39.0 34.0 39.0 11 37.22012826663449 39.0 37.0 39.0 34.0 39.0 12 37.112638001418645 39.0 37.0 39.0 34.0 39.0 13 37.124463961431715 39.0 37.0 39.0 33.0 39.0 14 38.22875786214427 40.0 38.0 41.0 33.0 41.0 15 38.23037664711371 40.0 37.0 41.0 33.0 41.0 16 38.10346979200085 40.0 37.0 41.0 33.0 41.0 17 38.04484917338953 40.0 37.0 41.0 33.0 41.0 18 37.889660673237955 40.0 37.0 41.0 33.0 41.0 19 37.73029865111064 39.0 37.0 41.0 33.0 41.0 20 37.82333463817213 40.0 36.0 41.0 33.0 41.0 21 37.79914999072878 40.0 36.0 41.0 33.0 41.0 22 37.91344798255244 40.0 36.0 41.0 33.0 41.0 23 37.8875297635691 40.0 36.0 41.0 33.0 41.0 24 37.80588119295623 40.0 36.0 41.0 33.0 41.0 25 37.51508560429243 39.0 35.0 41.0 33.0 41.0 26 37.61439364729913 39.0 36.0 41.0 33.0 41.0 27 37.64656626275529 40.0 36.0 41.0 33.0 41.0 28 37.50837500478277 40.0 35.0 41.0 33.0 41.0 29 37.51428504154391 40.0 35.0 41.0 33.0 41.0 30 37.286442528718716 39.0 35.0 41.0 33.0 41.0 31 37.204935233884996 39.0 35.0 41.0 32.0 41.0 32 37.09748617410474 39.0 35.0 41.0 32.0 41.0 33 37.00045031654604 40.0 35.0 41.0 32.0 41.0 34 36.819352427147315 39.0 35.0 41.0 31.0 41.0 35 36.79726631367343 40.0 35.0 41.0 31.0 41.0 36 36.60726216369742 39.0 35.0 41.0 31.0 41.0 37 36.53508790002384 39.0 35.0 41.0 31.0 41.0 38 36.412584140027846 39.0 35.0 41.0 30.0 41.0 39 36.30320431126586 39.0 35.0 41.0 30.0 41.0 40 36.308113644591344 39.0 35.0 41.0 30.0 41.0 41 36.0815573300055 39.0 35.0 41.0 30.0 41.0 42 36.09892542110484 39.0 35.0 41.0 30.0 41.0 43 36.05372894475823 39.0 35.0 41.0 30.0 41.0 44 36.163582636029446 39.0 35.0 41.0 30.0 41.0 45 36.10740785434467 39.0 35.0 41.0 30.0 41.0 46 36.02045555552285 38.0 35.0 41.0 30.0 41.0 47 35.88144901857482 38.0 35.0 40.0 30.0 41.0 48 35.84223910336972 38.0 35.0 40.0 30.0 41.0 49 35.825094698920715 38.0 35.0 40.0 30.0 41.0 50 35.68142606126071 38.0 35.0 40.0 30.0 41.0 51 34.87960654695507 37.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 8.0 9 10.0 10 9.0 11 11.0 12 8.0 13 12.0 14 17.0 15 21.0 16 27.0 17 56.0 18 107.0 19 248.0 20 498.0 21 981.0 22 1503.0 23 1939.0 24 2284.0 25 2776.0 26 3223.0 27 3658.0 28 3926.0 29 4036.0 30 4604.0 31 5948.0 32 7790.0 33 11187.0 34 20308.0 35 26398.0 36 27860.0 37 41936.0 38 69400.0 39 98926.0 40 44.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.314385700536555 18.072115398765604 22.57910707821086 15.034391822486985 2 29.448053190330853 31.04446949473306 23.45354528624533 16.053932028690756 3 29.463063741865607 29.56902057622858 25.468196761841412 15.499718920064398 4 27.33068245031066 22.80514832485188 31.9330352806826 17.931133944154862 5 30.474657185492156 26.36765255576714 23.34876575004194 19.808924508698762 6 24.490744964842932 37.59907699824288 23.216319707088218 14.693858329825964 7 72.88947230553242 6.008046832920789 16.226406209070493 4.8760746524763 8 72.71817542331227 11.84862300264009 10.210118289032586 5.223083285015054 9 66.8328619235286 7.52352388885128 11.818896224110476 13.824717963509645 10 42.92193630228308 22.25446711070429 18.33288694111449 16.490709645898146 11 33.415842312684504 22.22297438493529 23.924170225540895 20.437013076839307 12 26.15721050974067 19.911643773122872 29.386245036952445 24.54490068018401 13 25.99150579377857 20.820223627785413 33.20628324027772 19.981987338158294 14 22.023716671424914 27.509631770568134 27.97054400004709 22.49610755795986 15 19.98316463631788 21.11631411492196 35.118509775989594 23.782011472770563 16 21.760590532756847 21.827402203313508 28.952116340604128 27.459890923325514 17 21.741459437663536 24.173757435373687 29.490141599536145 24.594641527426635 18 22.12555296222933 22.26771171499966 29.460709145546428 26.14602617722458 19 23.603650801592885 23.241337292979477 26.53365159626914 26.621360309158497 20 26.293777096252953 23.28872354390292 29.19876030503795 21.218739054806175 21 24.28972130409317 27.698588125182113 26.620771660078702 21.39091891064601 22 22.95142762118077 21.682888854223997 28.719011305005576 26.64667221958965 23 24.000988930454053 25.605057672893594 26.654030333087082 23.73992306356527 24 23.173053999723333 24.015705157448913 27.337746239268192 25.47349460355956 25 21.991635296576124 29.191696516080423 25.58062873608213 23.236039451261327 26 21.63903449777932 24.356532974649827 28.859992759616322 25.144439767954534 27 26.32556414656185 24.21084232740073 25.5005724612301 23.96302106480732 28 21.919820108841215 25.06820971212117 29.81066102348415 23.201309155553464 29 23.96213809118763 23.556558875209337 28.17392225711603 24.307380776487 30 23.797610673385115 27.138488525757815 26.11129588151671 22.95260491934036 31 25.904091405429106 23.080930418735523 25.362239927478434 25.652738248356933 32 26.169277815876452 25.001103717024613 25.554139527491383 23.275478939607545 33 25.193297641577463 22.25446711070429 24.458663589994142 28.093571657724105 34 24.45572034459517 24.865125779592127 25.401385091284755 25.27776878452795 35 26.35646822325105 22.268594688619352 24.830984132964055 26.543952955165544 36 24.01393921020953 26.20518540974391 26.758221220210675 23.022654159835884 37 24.96637342131675 23.303734095437676 29.320022015475583 22.40987046776999 38 21.95366743092939 23.733447923687535 26.73202633615983 27.58085830922325 39 25.19653521151633 23.123313152480716 28.11947221723506 23.5606794187679 40 23.12390180156051 22.23886791008974 27.11141066808727 27.525819620262475 41 22.96496655001604 22.968498444494806 26.666097639222862 27.40043736626629 42 21.927178222338643 22.642681178828646 29.56960922530838 25.86053137352433 43 24.0374851734013 21.714087255453098 29.212004909333327 25.036422661812274 44 24.75151650719182 21.370021868313312 28.827617060227627 25.050844564267233 45 22.88932514326247 21.358837535797225 28.172744958956443 27.579092361983864 46 25.891141125673634 24.390968945817797 26.259341125084983 23.458548803423582 47 21.933653362216383 23.26576622979094 31.434155185556907 23.366425222435772 48 23.238394047580506 23.39262010648662 27.549954232534045 25.81903161339883 49 22.934356797866737 21.588999325996806 30.85786773643826 24.618776139698202 50 22.740691250614404 22.224740332174676 29.18581002528248 25.848758391928445 51 23.105359355546987 22.16823002051442 26.20724568152319 28.519164942415404 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 65.0 1 292.5 2 520.0 3 1877.0 4 3234.0 5 2163.5 6 1093.0 7 1091.0 8 1089.0 9 1104.5 10 1120.0 11 1089.5 12 1059.0 13 1100.0 14 1141.0 15 1092.0 16 1043.0 17 955.5 18 868.0 19 823.5 20 779.0 21 929.5 22 1080.0 23 1055.5 24 1031.0 25 1230.0 26 1771.0 27 2113.0 28 2396.5 29 2680.0 30 3091.0 31 3502.0 32 4075.0 33 4648.0 34 5361.0 35 6074.0 36 6494.5 37 6915.0 38 7340.5 39 7766.0 40 9259.0 41 10752.0 42 11946.5 43 13141.0 44 14995.5 45 16850.0 46 28737.5 47 40625.0 48 35236.0 49 29847.0 50 29000.0 51 28153.0 52 25289.0 53 22425.0 54 20920.5 55 19416.0 56 18873.0 57 18330.0 58 17528.0 59 16726.0 60 16305.5 61 15885.0 62 14973.5 63 14062.0 64 12599.5 65 11137.0 66 9927.5 67 8718.0 68 8065.0 69 7412.0 70 6524.0 71 5636.0 72 5005.5 73 4375.0 74 3697.0 75 2421.0 76 1823.0 77 1529.5 78 1236.0 79 861.0 80 486.0 81 351.5 82 217.0 83 185.0 84 153.0 85 93.0 86 33.0 87 39.5 88 46.0 89 26.0 90 6.0 91 4.5 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 339761.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.56359449939509 #Duplication Level Percentage of deduplicated Percentage of total 1 71.75301102702345 25.517949882756863 2 7.74806839589189 5.510983251701555 3 3.4292377137226007 3.6586805847858983 4 2.3520808403567592 3.3459379694497695 5 2.01420403869532 3.581616783560213 6 1.7826660763051334 3.8038808079326083 7 1.561926938802172 3.8883415402469352 8 1.4590846814017553 4.151223675972088 9 1.3023905522585775 4.168592053233055 >10 6.5135988686156665 32.54732979052265 >50 0.045596016643232776 1.066100840004066 >100 0.029844665439206906 2.1967691811035106 >500 0.003316073937689657 0.9773573795944355 >1k 0.0041450924221120705 4.008491985811748 >5k 8.290184844224142E-4 1.5767442733245973 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG 5348 1.5740476393700278 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC 4508 1.3268150258564109 No Hit GAATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC 4505 1.3259320522367193 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1990 0.5857058343953544 No Hit GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC 1548 0.4556143877608083 No Hit GAATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT 1045 0.30756914419253534 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTCGCTT 965 0.2840231810007623 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTA 887 0.2610658668887836 No Hit GAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT 864 0.2542964024711489 No Hit GAATGATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTC 599 0.17630039939840064 No Hit GAATGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT 485 0.14274740185012408 No Hit CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC 478 0.1406871300708439 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC 423 0.12449928037649996 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 422 0.12420495583660279 No Hit CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT 413 0.12155603497752833 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC 351 0.10330791350390421 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.12391063129670563 0.0 2 0.0 0.0 0.0 0.8344100706084571 0.0 3 0.0 0.0 0.0 1.0695753779862904 0.0 4 0.0 0.0 0.0 1.5861149455058114 0.0 5 0.0 0.0 0.0 3.685531888592275 0.0 6 0.0 0.0 0.0 4.560264421166644 0.0 7 0.0 0.0 0.0 5.458542916932785 0.0 8 0.0 0.0 0.0 6.571678326823855 0.0 9 0.0 0.0 0.0 7.055547870414792 0.0 10 0.0 0.0 0.0 9.473129641130088 0.0 11 0.0 0.0 0.0 10.566545306848049 0.0 12 0.0 0.0 0.0 12.841085351173325 0.0 13 0.0 0.0 0.0 13.287870002737218 0.0 14 0.0 0.0 0.0 13.505375837721221 0.0 15 0.0 0.0 0.0 14.040457851254263 0.0 16 0.0 0.0 0.0 14.606443941476508 0.0 17 0.0 0.0 0.0 15.192738424951656 0.0 18 0.0 0.0 0.0 15.828185106589633 0.0 19 0.0 0.0 0.0 16.505131548353106 0.0 20 0.0 0.0 0.0 16.905412922613248 0.0 21 0.0 0.0 0.0 17.300102130615343 0.0 22 0.0 0.0 0.0 17.748947053958517 0.0 23 0.0 0.0 0.0 18.162473032514033 0.0 24 0.0 0.0 0.0 18.512719234991657 0.0 25 0.0 0.0 0.0 18.82764649268162 0.0 26 0.0 0.0 0.0 19.140219154052406 0.0 27 0.0 0.0 0.0 19.474277506835687 0.0 28 0.0 0.0 0.0 19.768307722192954 0.0 29 0.0 0.0 0.0 20.059689016691145 0.0 30 0.0 0.0 0.0 20.410523868248564 2.94324539897163E-4 31 0.0 0.0 0.0 20.72751139771781 2.94324539897163E-4 32 0.0 0.0 0.0 21.01771539405641 2.94324539897163E-4 33 0.0 0.0 0.0 21.312039933953574 2.94324539897163E-4 34 0.0 0.0 0.0 21.608424745630018 2.94324539897163E-4 35 0.0 0.0 0.0 21.901277662827695 2.94324539897163E-4 36 0.0 0.0 0.0 22.18677246652794 2.94324539897163E-4 37 0.0 0.0 0.0 22.468441051209528 2.94324539897163E-4 38 0.0 0.0 0.0 22.74510611871286 2.94324539897163E-4 39 0.0 0.0 0.0 23.028834975173726 2.94324539897163E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTTA 20 7.026265E-4 45.0 38 GATCATC 20 7.026265E-4 45.0 22 TCGGATT 20 7.026265E-4 45.0 31 ATACGGA 20 7.026265E-4 45.0 28 CGTTTAT 20 7.026265E-4 45.0 39 TACACGG 30 2.1609721E-6 44.999996 2 CGAATGC 105 0.0 42.85714 45 TATGCCC 100 0.0 42.75 10 CGTGTAC 100 0.0 42.75 18 ACCTATG 80 0.0 42.1875 34 TACGAAT 80 0.0 42.1875 12 CATCGAA 80 0.0 42.1875 42 ATCGAAT 80 0.0 42.1875 43 GATGAAT 600 0.0 41.25 20 CGATGAA 580 0.0 41.12069 19 TCGTGTA 105 0.0 40.714283 17 CATATGC 50 1.0768417E-9 40.5 33 CGTTTTT 1040 0.0 40.45673 1 ACACGCG 45 1.9213985E-8 40.0 36 CTACGAA 85 0.0 39.705883 11 >>END_MODULE