##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551629_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444137
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94580275905858	33.0	31.0	34.0	30.0	34.0
2	32.046508172028	33.0	31.0	34.0	30.0	34.0
3	32.01813404422509	33.0	31.0	34.0	30.0	34.0
4	35.71333169720154	37.0	35.0	37.0	33.0	37.0
5	35.71246259600078	37.0	35.0	37.0	35.0	37.0
6	35.58097614024501	37.0	35.0	37.0	33.0	37.0
7	35.861779135717136	37.0	35.0	37.0	35.0	37.0
8	35.58823516167309	37.0	35.0	37.0	33.0	37.0
9	37.369440060161615	39.0	37.0	39.0	34.0	39.0
10	37.137270707011574	39.0	37.0	39.0	34.0	39.0
11	37.205213706581524	39.0	37.0	39.0	34.0	39.0
12	37.12191958787491	39.0	37.0	39.0	34.0	39.0
13	37.14457475958994	39.0	37.0	39.0	33.0	39.0
14	38.23194194584104	40.0	38.0	41.0	33.0	41.0
15	38.24877008670748	40.0	38.0	41.0	33.0	41.0
16	38.10517700619403	40.0	37.0	41.0	33.0	41.0
17	38.01598830991338	40.0	37.0	41.0	33.0	41.0
18	37.84055145146655	39.0	37.0	41.0	33.0	41.0
19	37.63536926669023	39.0	37.0	41.0	33.0	41.0
20	37.728302303118184	39.0	36.0	41.0	33.0	41.0
21	37.7124806084609	39.0	36.0	41.0	33.0	41.0
22	37.86772549911401	40.0	36.0	41.0	33.0	41.0
23	37.836395076294025	40.0	36.0	41.0	33.0	41.0
24	37.72381945210599	40.0	36.0	41.0	33.0	41.0
25	37.39230912984057	39.0	35.0	41.0	33.0	41.0
26	37.55267856539761	39.0	35.0	41.0	33.0	41.0
27	37.58274811600925	40.0	35.0	41.0	33.0	41.0
28	37.42825749712364	40.0	35.0	41.0	33.0	41.0
29	37.47396411467633	40.0	35.0	41.0	33.0	41.0
30	37.191411659015124	39.0	35.0	41.0	32.0	41.0
31	37.04779831448404	39.0	35.0	41.0	32.0	41.0
32	36.97701384933028	39.0	35.0	41.0	31.0	41.0
33	36.86476019786687	39.0	35.0	41.0	31.0	41.0
34	36.5657375989841	39.0	35.0	41.0	31.0	41.0
35	36.47346652046553	39.0	35.0	41.0	30.0	41.0
36	36.336186807223896	39.0	35.0	41.0	30.0	41.0
37	36.30838232347226	39.0	35.0	41.0	30.0	41.0
38	36.10497661757521	39.0	35.0	41.0	30.0	41.0
39	36.044720435361164	39.0	35.0	41.0	29.0	41.0
40	35.94349491260579	39.0	35.0	41.0	29.0	41.0
41	35.73293375692636	38.0	35.0	40.0	28.0	41.0
42	35.78468130329155	39.0	35.0	41.0	28.0	41.0
43	35.79016159428284	39.0	35.0	41.0	29.0	41.0
44	35.89147267622378	39.0	35.0	41.0	29.0	41.0
45	35.837252919707204	38.0	35.0	41.0	29.0	41.0
46	35.7426199573555	38.0	35.0	40.0	29.0	41.0
47	35.579559910568136	38.0	35.0	40.0	28.0	41.0
48	35.57777892857384	38.0	35.0	40.0	29.0	41.0
49	35.57290880966909	38.0	35.0	40.0	29.0	41.0
50	35.40396093998023	37.0	35.0	40.0	28.0	41.0
51	34.578963247826685	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	8.0
11	16.0
12	12.0
13	13.0
14	10.0
15	25.0
16	43.0
17	66.0
18	162.0
19	328.0
20	703.0
21	1298.0
22	1933.0
23	2553.0
24	2979.0
25	3686.0
26	4738.0
27	5735.0
28	5888.0
29	6091.0
30	6947.0
31	8392.0
32	10897.0
33	15014.0
34	26222.0
35	34341.0
36	37540.0
37	55318.0
38	92153.0
39	120956.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.372036105976306	17.286107664977248	21.485487586037642	15.8563686430088
2	30.70358920783452	30.102873662856283	22.943596232693967	16.249940896615232
3	29.574207958355192	28.617521170269534	26.662493780072367	15.145777091302909
4	26.90408590142231	23.042214451847066	32.777498834818985	17.27620081191164
5	29.79936370984629	26.376545975678678	24.46317239950736	19.36091791496768
6	24.74506740037421	36.679448008159646	24.493568425958657	14.08191616550749
7	71.83796891499695	6.325750838142286	17.17668196975258	4.65959827710819
8	71.80306977351583	10.778205823878668	11.98639158637988	5.432332816225624
9	65.8605340244114	7.660248977229998	13.237582097415887	13.241634900942728
10	40.9454740316614	22.69952739807762	20.134327921339587	16.22067064892139
11	31.791541799039486	22.28208863481313	25.859363214503635	20.067006351643748
12	26.013594904275035	19.255094711766866	31.13543793919444	23.595872444763664
13	25.393515964668563	20.508761936069277	34.17639151883316	19.92133058042901
14	21.02977234501967	26.327236866102126	29.593796508734915	23.04919428014329
15	19.340203585830498	21.805659064657977	35.88037024611776	22.973767103393772
16	21.60482013432792	22.231428590727635	29.304021056565883	26.859730218378562
17	21.735860781695738	24.0905396307896	30.522338827884187	23.651260759630475
18	21.985783665850853	22.364720795610364	30.263634869420926	25.38586066911786
19	22.82088634813132	24.099320705097753	27.854018016963234	25.22577492980769
20	25.337452182547278	23.67557758079151	30.03194960113659	20.955020635524622
21	23.60487867482331	27.615803231885653	27.8195691869851	20.959748906305936
22	22.686693520242628	22.06571395763019	29.421552358844234	25.826040163282947
23	23.984040960334312	25.01660523667247	28.270331001470268	22.72902280152295
24	23.295289516523056	23.89758115176173	27.955563260885718	24.851566070829495
25	21.81083764694227	28.45991214422577	26.558922134386464	23.170328074445496
26	21.78201771075141	23.603302584562872	29.39858647219214	25.216093232493574
27	24.96932252885934	23.89555474999831	26.856578037857687	24.27854468328466
28	21.336209322799046	25.6679808257362	29.513641061204087	23.482168790260662
29	25.144043392016428	22.98479973521684	27.0889387733965	24.78221809937024
30	23.441640754992264	26.29571506089337	26.765615114255286	23.497029069859074
31	24.845712021290726	26.290536478609077	23.634599234020133	25.229152266080064
32	26.656864886285085	27.694382589156046	23.282680794439553	22.366071730119312
33	25.762321085610974	24.694407356288714	22.815482610095533	26.727788948004783
34	24.877909293753955	27.49624552784389	24.22180543390891	23.40403974449325
35	24.358700130815492	26.798938165475967	23.240126357407735	25.602235346300805
36	24.573723873489488	29.08044139533522	22.57051315247322	23.775321578702066
37	22.44059828386286	28.962234625802395	25.094509126688386	23.502657963646353
38	23.415747843570788	29.031357441510163	23.07441172431029	24.478482990608754
39	23.859079518256753	28.734151849541924	24.25557879663257	23.151189835568754
40	24.134219846578873	25.878276297628883	26.736795178064426	23.25070867772782
41	21.02436860698388	27.20894678894125	24.47510565433639	27.291578949738483
42	21.8151156062206	26.351553687263163	27.496020372092396	24.337310334423837
43	23.829358959059928	23.666571350731868	25.732150214911165	26.771919475297036
44	24.141424830626587	23.749428667280593	26.70482306135269	25.404323440740136
45	22.61329274525653	23.626268471214964	26.742424071851705	27.018014711676802
46	25.851482763201446	25.89583844624519	25.2888185402252	22.963860250328164
47	21.350394135142984	24.868227596439837	30.90510360541905	22.876274662998128
48	22.69322303703587	25.32551892771825	26.86783582543224	25.113422209813642
49	22.369223910640184	23.630771586244784	29.940311210279713	24.05969329283532
50	22.80444997827247	22.807377003041854	28.925534238309353	25.462638780376324
51	21.982181173826994	23.37184247203003	26.440265053350654	28.205711300792323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	368.0
2	628.0
3	3751.0
4	6874.0
5	4501.0
6	2128.0
7	2062.5
8	1997.0
9	1887.5
10	1778.0
11	1774.5
12	1771.0
13	1711.5
14	1652.0
15	1576.5
16	1501.0
17	1433.0
18	1365.0
19	1296.5
20	1228.0
21	1278.5
22	1329.0
23	1420.0
24	1511.0
25	1701.5
26	2417.5
27	2943.0
28	3574.5
29	4206.0
30	4791.5
31	5377.0
32	5999.5
33	6622.0
34	7451.0
35	8280.0
36	8728.5
37	9177.0
38	9853.5
39	10530.0
40	11982.5
41	13435.0
42	15015.0
43	16595.0
44	19299.0
45	22003.0
46	37068.0
47	52133.0
48	45036.5
49	37940.0
50	37461.0
51	36982.0
52	33209.5
53	29437.0
54	27225.0
55	25013.0
56	24073.0
57	23133.0
58	22611.5
59	22090.0
60	21246.0
61	20402.0
62	19222.0
63	18042.0
64	16466.0
65	14890.0
66	13036.5
67	11183.0
68	10196.5
69	9210.0
70	7705.0
71	6200.0
72	5530.0
73	4860.0
74	4142.5
75	2745.0
76	2065.0
77	1608.5
78	1152.0
79	848.5
80	545.0
81	418.5
82	292.0
83	204.5
84	117.0
85	89.5
86	62.0
87	46.5
88	31.0
89	16.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	444137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.19876849172316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.02356343542407	26.071642987478533
2	7.8189473407421035	5.660725292729957
3	3.5123401976700954	3.814271690388989
4	2.4673045963581433	3.572535515285316
5	2.0441877671566018	3.699853986845716
6	1.8088044984584533	3.928589717189098
7	1.5734193596561363	3.986909019841144
8	1.3735699464485598	3.9777232398944
9	1.258904171123933	4.10137025794019
>10	6.029177946408501	30.764864768742374
>50	0.055489485480902975	1.3652056328968536
>100	0.025562571963112606	1.8308064298329108
>500	0.0031173868247698303	0.7217602271456667
>1k	0.0037408641897237966	2.5015604620645933
>5k	0.0018704320948618983	4.002180771724237
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCG	5951	1.3399018771235	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC	5944	1.3383257868630625	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC	5838	1.3144592772050065	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4344	0.9780765844773122	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	2089	0.4703503648648953	Illumina Single End Adapter 1 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT	1332	0.29990746098613713	Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCT	1237	0.27851766459448324	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAGAACT	1041	0.23438713730222885	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTA	1041	0.23438713730222885	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTC	926	0.20849422588075303	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	580	0.13059033586483448	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCC	570	0.12833877834992355	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	570	0.12833877834992355	No Hit
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	552	0.12428597482308386	Illumina Single End Adapter 1 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTT	486	0.10942569522467166	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13329220488272764	0.0
2	0.0	0.0	0.0	0.821818492942493	0.0
3	0.0	0.0	0.0	1.0753438691214647	0.0
4	0.0	0.0	0.0	1.6830392423959273	0.0
5	2.2515575149109396E-4	0.0	0.0	3.86524878584761	0.0
6	2.2515575149109396E-4	0.0	0.0	4.769249128084352	0.0
7	2.2515575149109396E-4	0.0	0.0	5.726386227672993	0.0
8	2.2515575149109396E-4	0.0	0.0	6.997390444840218	0.0
9	2.2515575149109396E-4	0.0	0.0	7.595854432303546	0.0
10	2.2515575149109396E-4	0.0	0.0	9.904151196590242	0.0
11	2.2515575149109396E-4	0.0	0.0	10.977243508196795	0.0
12	2.2515575149109396E-4	0.0	0.0	13.199981086916875	0.0
13	2.2515575149109396E-4	0.0	0.0	13.664927713745984	0.0
14	2.2515575149109396E-4	0.0	0.0	13.904043121829526	0.0
15	2.2515575149109396E-4	0.0	0.0	14.41739823522922	0.0
16	2.2515575149109396E-4	0.0	0.0	14.950116743257148	0.0
17	2.2515575149109396E-4	0.0	0.0	15.532369516613118	0.0
18	2.2515575149109396E-4	0.0	0.0	16.20130725429316	0.0
19	2.2515575149109396E-4	0.0	0.0	16.827015087686906	0.0
20	2.2515575149109396E-4	0.0	0.0	17.247831187223763	0.0
21	2.2515575149109396E-4	0.0	0.0	17.652210916901765	0.0
22	2.2515575149109396E-4	0.0	0.0	18.12211097026368	0.0
23	4.503115029821879E-4	0.0	0.0	18.53954973352817	0.0
24	6.754672544732819E-4	0.0	0.0	18.90452720669523	0.0
25	6.754672544732819E-4	0.0	0.0	19.207812003953734	0.0
26	6.754672544732819E-4	0.0	0.0	19.516275383496534	0.0
27	6.754672544732819E-4	0.0	0.0	19.85558510099361	0.0
28	6.754672544732819E-4	0.0	0.0	20.160445988512553	0.0
29	6.754672544732819E-4	0.0	0.0	20.474763417594122	0.0
30	6.754672544732819E-4	0.0	0.0	20.83095981645303	0.0
31	6.754672544732819E-4	0.0	0.0	21.154958942848715	0.0
32	6.754672544732819E-4	0.0	0.0	21.460945609125112	0.0
33	6.754672544732819E-4	0.0	0.0	21.773011480691768	0.0
34	6.754672544732819E-4	0.0	0.0	22.073144097429395	0.0
35	6.754672544732819E-4	0.0	0.0	22.387686682262455	0.0
36	6.754672544732819E-4	0.0	0.0	22.68286587246728	0.0
37	6.754672544732819E-4	0.0	0.0	22.992905342270515	0.0
38	9.006230059643758E-4	0.0	0.0	23.30249450057077	0.0
39	0.0013509345089465637	0.0	0.0	23.6481085791096	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGA	20	7.028606E-4	45.0	37
GCCGTTA	20	7.028606E-4	45.0	45
TAGTAAG	35	1.2096825E-7	45.0	1
CGTTTTT	1945	0.0	43.1491	1
ATAGCGG	70	0.0	41.785713	2
CGAATAT	65	0.0	41.53846	14
CGAATGC	90	0.0	40.0	45
CTCGAAT	90	0.0	40.0	43
TATGGGC	120	0.0	39.375004	4
TAGCTAG	40	3.4518416E-7	39.375	1
CGATGAA	750	0.0	39.3	19
TACGGCT	760	0.0	38.782898	7
CCGATGA	745	0.0	38.65772	18
GATGAAT	810	0.0	38.61111	20
CATATTA	70	0.0	38.571426	28
AGACCTA	70	0.0	38.571426	32
TTAGTCA	70	0.0	38.571426	32
CTAGGCG	35	6.2396357E-6	38.571426	1
ACGGCTG	765	0.0	38.529415	8
AACGGGC	65	9.094947E-12	38.07692	4
>>END_MODULE