SRR3551628_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551628_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240644
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG	7152	2.972025066072705	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	6319	2.6258705806087	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	6163	2.5610445305097986	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	2421	1.006050431342564	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1836	0.7629527434716843	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCT	1544	0.6416116753378435	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTAGGGTT	1343	0.5580858030950283	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	1197	0.4974152690281079	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTA	1099	0.4566912119146955	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC	1024	0.42552484167483917	No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	851	0.35363441432157044	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	687	0.2854839513970845	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCC	428	0.17785608616878043	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTT	399	0.16580508967603597	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCGATCGAATGC	392	0.16289622845364937	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATG	327	0.13588537424577385	No Hit
TCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	322	0.1338076162297834	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGT	284	0.11801665530825618	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTAGGGT	284	0.11801665530825618	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	241	0.10014793637073852	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	100	0.0	45.0	40
ACCGCTC	20	7.022158E-4	45.0	39
GTCAGCG	25	3.8815848E-5	45.0	35
CGCTCGA	20	7.022158E-4	45.0	41
TATTAGT	20	7.022158E-4	45.0	30
CTCGAAT	20	7.022158E-4	45.0	43
CGATCGA	95	0.0	45.0	41
TTCGAAG	20	7.022158E-4	45.0	1
TATCGAA	20	7.022158E-4	45.0	41
TACCGTT	25	3.8815848E-5	45.0	15
TAGTAGG	35	1.206572E-7	45.0	2
TAGTGGT	30	2.1583255E-6	44.999996	15
TACGGCT	680	0.0	42.683823	7
CGAATGC	95	0.0	42.63158	45
ATCGAAT	90	0.0	42.500004	43
CCGATGA	715	0.0	42.482517	18
CGTTTTT	940	0.0	42.36702	1
CGATGAA	725	0.0	42.206898	19
GATGAAT	780	0.0	41.826927	20
TCGAATG	110	0.0	40.909092	44

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content