##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551628_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240644
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.899843752597196	33.0	31.0	34.0	30.0	34.0
2	31.98743371951929	33.0	31.0	34.0	30.0	34.0
3	31.980722561127642	33.0	31.0	34.0	30.0	34.0
4	35.69023536842805	37.0	35.0	37.0	35.0	37.0
5	35.66760442811788	37.0	35.0	37.0	35.0	37.0
6	35.561597214142054	37.0	35.0	37.0	33.0	37.0
7	35.80884626252888	37.0	35.0	37.0	35.0	37.0
8	35.38297651302339	37.0	35.0	37.0	33.0	37.0
9	37.10522182144579	39.0	37.0	39.0	34.0	39.0
10	37.00273432954904	39.0	37.0	39.0	33.0	39.0
11	37.124702880603714	39.0	37.0	39.0	34.0	39.0
12	37.02663270224897	39.0	37.0	39.0	33.0	39.0
13	36.99049217931883	39.0	37.0	39.0	33.0	39.0
14	38.0661641262612	40.0	37.0	41.0	33.0	41.0
15	37.99346752879772	40.0	37.0	41.0	33.0	41.0
16	37.94784827379864	40.0	37.0	41.0	33.0	41.0
17	37.81043782516913	40.0	36.0	41.0	33.0	41.0
18	37.68424726982597	39.0	36.0	41.0	33.0	41.0
19	37.51244992603181	39.0	36.0	41.0	32.0	41.0
20	37.48166170775087	39.0	35.0	41.0	32.0	41.0
21	37.62043516563887	39.0	36.0	41.0	33.0	41.0
22	37.70609281760609	40.0	36.0	41.0	33.0	41.0
23	37.66032396402985	40.0	36.0	41.0	33.0	41.0
24	37.56467229600572	40.0	35.0	41.0	33.0	41.0
25	37.401032230182345	39.0	35.0	41.0	33.0	41.0
26	37.521500639949465	39.0	36.0	41.0	33.0	41.0
27	37.46813550306677	39.0	35.0	41.0	33.0	41.0
28	37.33524210036402	39.0	35.0	41.0	32.0	41.0
29	37.39936171273749	40.0	35.0	41.0	33.0	41.0
30	37.15924768537757	39.0	35.0	41.0	32.0	41.0
31	37.06774737786938	39.0	35.0	41.0	31.0	41.0
32	36.869874170974555	39.0	35.0	41.0	31.0	41.0
33	36.72502119313177	39.0	35.0	41.0	31.0	41.0
34	36.48011170027094	39.0	35.0	41.0	30.0	41.0
35	36.51119496019015	39.0	35.0	41.0	30.0	41.0
36	36.00359036585163	39.0	35.0	41.0	28.0	41.0
37	35.996621565466	39.0	35.0	41.0	30.0	41.0
38	35.90050863516232	39.0	35.0	41.0	29.0	41.0
39	35.880724223334056	39.0	35.0	41.0	29.0	41.0
40	35.91835242100364	39.0	35.0	41.0	29.0	41.0
41	35.58277372384103	38.0	35.0	40.0	26.0	41.0
42	35.703645218663254	39.0	35.0	40.0	28.0	41.0
43	35.474667974269046	38.0	35.0	40.0	27.0	41.0
44	35.81213327571018	39.0	35.0	40.0	29.0	41.0
45	35.853031864496934	39.0	35.0	40.0	29.0	41.0
46	35.810363025880555	39.0	35.0	40.0	29.0	41.0
47	35.617937700503646	38.0	35.0	40.0	28.0	41.0
48	35.65861189142468	38.0	35.0	40.0	28.0	41.0
49	35.71072621798175	38.0	35.0	40.0	29.0	41.0
50	35.41315802596366	38.0	35.0	40.0	27.0	41.0
51	34.708394973487806	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	6.0
10	6.0
11	5.0
12	8.0
13	6.0
14	6.0
15	11.0
16	12.0
17	39.0
18	67.0
19	185.0
20	495.0
21	938.0
22	1484.0
23	1805.0
24	2046.0
25	2348.0
26	2660.0
27	2910.0
28	2869.0
29	3068.0
30	3635.0
31	4690.0
32	6103.0
33	8505.0
34	14260.0
35	18564.0
36	19196.0
37	28723.0
38	50465.0
39	65500.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.836688219943156	15.755639035255397	20.07903791492828	14.328634829873174
2	27.86024168481242	35.82428816010372	21.17817190538721	15.137298249696649
3	26.22753943584714	34.653263742291514	25.25390202955403	13.865294792307308
4	24.562424161832418	20.65374578215123	38.40569471917023	16.37813533684613
5	32.95822875284653	24.001013945911804	23.98646963979987	19.054287661441798
6	22.284370273100514	39.45496251724539	24.45562739981051	13.805039809843587
7	65.81298515649672	5.592077924236632	23.42215056265687	5.172786356609764
8	63.44018550223567	16.450441315802596	13.33795980784894	6.7714133741127975
9	57.9436844467346	7.688535762370971	14.194411662040194	20.173368128854243
10	40.88487558385	20.78381343395223	21.268346603281195	17.062964378916572
11	32.478682202755934	20.582270906401156	26.684646199365037	20.254400691477866
12	25.071890427353267	17.496800252655376	31.21166536460498	26.219643955386378
13	25.53897042934792	20.598061867322684	36.09522780538887	17.767739897940526
14	20.2685294459866	29.591845215338843	27.482089725902163	22.657535612772396
15	16.81612672661691	21.26045112282043	39.117950167051745	22.805471983510913
16	17.614401356360432	23.04482970695301	28.587041438805873	30.753727497880686
17	18.277621715064576	26.08043416831502	31.42567444025199	24.216269676368412
18	18.61089410082944	22.774721164874254	31.500889280430844	27.11349545386546
19	20.342082079752664	24.538737720450126	26.972207908778113	28.1469722910191
20	23.40303518890976	24.09908412426655	32.874702880603714	19.623177806219978
21	21.220558168913414	30.86218646631539	28.076328518475425	19.840926846295773
22	18.9021957746713	21.945280164890875	31.30267116570536	27.849852894732468
23	22.195442230016123	27.310466913781354	28.366383537507687	22.127707318694835
24	22.440202124299795	24.607719286581005	26.495154668306707	26.456923920812486
25	18.38774288991207	33.455228470271436	25.02285533817589	23.134173301640597
26	18.9670218247702	22.292265753561278	32.89880487358921	25.84190754807932
27	26.72287694685926	24.25574707867223	25.61501637273317	23.406359601735343
28	17.793919648942005	25.165805089676034	33.61355363108991	23.42672163029205
29	23.29000515283988	21.447033792656374	31.895247751865824	23.36771330263792
30	24.956782633267398	27.430145775502403	27.74887385515533	19.864197736074864
31	25.18492046342315	21.60328119545885	26.573278369707946	26.63851997141005
32	29.673293329565663	27.353268728910756	24.22707401805156	18.746363923472018
33	28.648958627682386	20.94795631721547	24.573644055118766	25.829440999983376
34	27.09812004454713	21.636940875317897	28.519722079087785	22.74521700104719
35	31.7053406692043	20.953774039660246	25.027426405811077	22.31345888532438
36	24.35963497947175	23.52188294742441	33.244959359053205	18.87352271405063
37	25.884709363208724	25.12175661973704	29.665397849104902	19.328136167949335
38	25.565150180349395	23.04856967138179	27.006283140240356	24.379997008028457
39	27.642077093133427	25.47081996642343	26.96888349595253	19.918219444490617
40	29.420637954821228	20.50497830820631	27.506191718887653	22.568192018084808
41	24.6746230946959	22.715297285616927	27.92340552849853	24.686674091188642
42	25.72056647994548	20.962500623327404	31.62139924535829	21.695533651368827
43	24.970911387776134	21.348132511095226	30.046874220840742	23.634081880287894
44	25.343245624241618	20.521600372334238	29.78341450441316	24.35173949901099
45	22.844533834211532	19.295307591296687	28.92072937617393	28.939429198317846
46	28.750768770465918	24.29647113578564	26.863333388740212	20.08942670500823
47	21.151576602782534	21.971044364289156	35.98884659497016	20.888532437958148
48	24.25824038829142	22.660028922391582	27.7999867023487	25.2817439869683
49	23.002027891823605	18.82614983128605	34.97490068316684	23.19692159372351
50	23.683532521068464	19.371353534681937	30.975632053988466	25.969481890261132
51	22.90395771346886	19.528016489087616	27.576419939828128	29.9916058576154
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	208.5
2	348.0
3	1572.0
4	2796.0
5	1838.5
6	881.0
7	847.5
8	814.0
9	851.5
10	889.0
11	847.5
12	806.0
13	748.0
14	690.0
15	685.5
16	681.0
17	649.5
18	618.0
19	631.5
20	645.0
21	647.5
22	650.0
23	771.5
24	893.0
25	1048.5
26	1296.0
27	1388.0
28	1663.0
29	1938.0
30	2327.0
31	2716.0
32	2976.5
33	3237.0
34	3557.0
35	3877.0
36	4351.0
37	4825.0
38	5089.5
39	5354.0
40	6384.0
41	7414.0
42	8395.5
43	9377.0
44	11119.0
45	12861.0
46	27514.5
47	42168.0
48	33184.5
49	24201.0
50	23484.0
51	22767.0
52	19278.0
53	15789.0
54	14736.0
55	13683.0
56	12121.0
57	10559.0
58	10078.0
59	9597.0
60	8968.5
61	8340.0
62	7589.5
63	6839.0
64	6591.5
65	6344.0
66	5425.5
67	4507.0
68	3946.5
69	3386.0
70	3002.5
71	2619.0
72	2233.5
73	1848.0
74	1557.5
75	1030.5
76	794.0
77	683.5
78	573.0
79	392.5
80	212.0
81	155.0
82	98.0
83	78.5
84	59.0
85	35.5
86	12.0
87	10.0
88	8.0
89	4.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	240644.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.42707069363874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.08973419492372	27.355346486926745
2	8.34610174760731	6.247402802480012
3	3.7294872648946327	4.187513505427104
4	2.49483711944574	3.734977809544389
5	2.1706304265760665	4.062016921261282
6	1.800901561077432	4.044148202323765
7	1.573290697932627	4.121856352121807
8	1.3356871627473186	3.9992686291783714
9	1.1069660027091244	3.7287445354964177
>10	4.253547398574379	21.546766177423912
>50	0.04774276641573957	1.1701933146058079
>100	0.03775009437523594	2.655374744435764
>500	0.0022205937867785844	0.6391183657186549
>1k	0.007772078253725046	4.348331975864763
>5k	0.003330890680167877	8.158940177191203
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG	7152	2.972025066072705	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	6319	2.6258705806087	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	6163	2.5610445305097986	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	2421	1.006050431342564	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1836	0.7629527434716843	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCT	1544	0.6416116753378435	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTAGGGTT	1343	0.5580858030950283	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	1197	0.4974152690281079	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTA	1099	0.4566912119146955	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC	1024	0.42552484167483917	No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	851	0.35363441432157044	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	687	0.2854839513970845	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCC	428	0.17785608616878043	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTT	399	0.16580508967603597	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCGATCGAATGC	392	0.16289622845364937	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATG	327	0.13588537424577385	No Hit
TCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	322	0.1338076162297834	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGT	284	0.11801665530825618	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTAGGGT	284	0.11801665530825618	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	241	0.10014793637073852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1555160319808513E-4	0.0	0.0	0.29421053506424427	0.0
2	4.1555160319808513E-4	0.0	0.0	1.9360549192998786	0.0
3	4.1555160319808513E-4	0.0	0.0	2.3466199032595867	0.0
4	4.1555160319808513E-4	0.0	0.0	3.3809278436196206	0.0
5	4.1555160319808513E-4	0.0	0.0	7.293346187729592	0.0
6	4.1555160319808513E-4	0.0	0.0	8.390402420172537	0.0
7	4.1555160319808513E-4	0.0	0.0	9.875168298399295	0.0
8	4.1555160319808513E-4	0.0	0.0	11.507454995761373	0.0
9	4.1555160319808513E-4	0.0	0.0	12.087149482222703	0.0
10	4.1555160319808513E-4	0.0	0.0	16.228952311298016	0.0
11	4.1555160319808513E-4	0.0	0.0	17.665929755156995	0.0
12	4.1555160319808513E-4	0.0	0.0	21.50396436229451	0.0
13	4.1555160319808513E-4	0.0	0.0	22.130616179917222	0.0
14	4.1555160319808513E-4	0.0	0.0	22.421086750552682	0.0
15	4.1555160319808513E-4	0.0	0.0	23.21853027708981	0.0
16	4.1555160319808513E-4	0.0	0.0	23.93868120543209	0.0
17	4.1555160319808513E-4	0.0	0.0	24.70952942936454	0.0
18	4.1555160319808513E-4	0.0	0.0	25.48785758215455	0.0
19	4.1555160319808513E-4	0.0	0.0	26.591147088645467	0.0
20	4.1555160319808513E-4	0.0	0.0	27.16169943983644	0.0
21	4.1555160319808513E-4	0.0	0.0	27.687372217882018	0.0
22	4.1555160319808513E-4	0.0	0.0	28.26332673991456	0.0
23	4.1555160319808513E-4	0.0	0.0	28.72832898389322	0.0
24	4.1555160319808513E-4	0.0	0.0	29.159255996409634	0.0
25	4.1555160319808513E-4	0.0	0.0	29.523279200811157	0.0
26	4.1555160319808513E-4	0.0	0.0	29.84450059008328	0.0
27	4.1555160319808513E-4	0.0	0.0	30.25381891923339	0.0
28	4.1555160319808513E-4	0.0	0.0	30.625737604095676	0.0
29	4.1555160319808513E-4	0.0	0.0	30.973138744369276	0.0
30	4.1555160319808513E-4	0.0	0.0	31.365419457788267	0.0
31	8.311032063961703E-4	0.0	0.0	31.70991173683948	0.0
32	8.311032063961703E-4	0.0	0.0	32.05648177390668	0.0
33	8.311032063961703E-4	0.0	0.0	32.36191220225728	0.0
34	8.311032063961703E-4	0.0	0.0	32.68978241718057	0.0
35	8.311032063961703E-4	0.0	0.0	33.017652632103854	0.0
36	8.311032063961703E-4	0.0	0.0	33.348847259852725	0.0
37	8.311032063961703E-4	0.0	0.0	33.65178437858413	0.0
38	8.311032063961703E-4	0.0	0.0	33.934775020362025	0.0
39	8.311032063961703E-4	0.0	0.0	34.237296587490235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	100	0.0	45.0	40
ACCGCTC	20	7.022158E-4	45.0	39
GTCAGCG	25	3.8815848E-5	45.0	35
CGCTCGA	20	7.022158E-4	45.0	41
TATTAGT	20	7.022158E-4	45.0	30
CTCGAAT	20	7.022158E-4	45.0	43
CGATCGA	95	0.0	45.0	41
TTCGAAG	20	7.022158E-4	45.0	1
TATCGAA	20	7.022158E-4	45.0	41
TACCGTT	25	3.8815848E-5	45.0	15
TAGTAGG	35	1.206572E-7	45.0	2
TAGTGGT	30	2.1583255E-6	44.999996	15
TACGGCT	680	0.0	42.683823	7
CGAATGC	95	0.0	42.63158	45
ATCGAAT	90	0.0	42.500004	43
CCGATGA	715	0.0	42.482517	18
CGTTTTT	940	0.0	42.36702	1
CGATGAA	725	0.0	42.206898	19
GATGAAT	780	0.0	41.826927	20
TCGAATG	110	0.0	40.909092	44
>>END_MODULE