SRR3551627_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551627_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	264917
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGC	7461	2.8163538013793	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCG	7210	2.7216071448793397	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTC	6286	2.372818656409366	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	2976	1.123370716111084	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2399	0.9055666491769121	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCT	1663	0.6277437839021278	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAAGAGT	1439	0.5431889988184979	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	1191	0.44957477247590755	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTA	1181	0.4458000052846741	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTC	1068	0.40314513602373575	No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT	734	0.27706791183653745	No Hit
CCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	589	0.222333787563652	No Hit
TCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	577	0.21780406693417184	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	441	0.16646723313339648	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCC	436	0.16457984953777977	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATG	425	0.16042760562742295	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTT	420	0.15854022203180618	No Hit
ACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	355	0.13400423528878858	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAAAGAG	306	0.11550787605174452	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTAT	301	0.11362049245612776	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGT	280	0.10569348135453746	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.023449E-4	45.0	25
TTGATCC	20	7.023449E-4	45.0	17
CAGCGGA	50	2.1827873E-11	45.0	37
AACCGCG	20	7.023449E-4	45.0	1
TGATAAG	20	7.023449E-4	45.0	26
TACCCTT	20	7.023449E-4	45.0	23
CTCACGA	20	7.023449E-4	45.0	24
TACCCGC	20	7.023449E-4	45.0	28
CGTATGG	25	3.8826536E-5	45.0	2
GTCGAAT	70	0.0	45.0	43
CAAGTCG	20	7.023449E-4	45.0	15
GGTCGAA	20	7.023449E-4	45.0	42
ATTACGG	20	7.023449E-4	45.0	2
TATATCA	25	3.8826536E-5	45.0	41
GTTACGG	20	7.023449E-4	45.0	2
TGTCTAT	25	3.8826536E-5	45.0	37
CGAGGGT	25	3.8826536E-5	45.0	4
GCTTAAG	20	7.023449E-4	45.0	1
GCGGGTT	45	3.8198777E-10	45.0	5
TCTAAAC	20	7.023449E-4	45.0	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content