##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551627_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	264917
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83747362381425	33.0	31.0	34.0	30.0	34.0
2	31.936508415843452	33.0	31.0	34.0	30.0	34.0
3	31.938361826534347	33.0	31.0	34.0	30.0	34.0
4	35.64346191448642	37.0	35.0	37.0	33.0	37.0
5	35.63969092206238	37.0	35.0	37.0	35.0	37.0
6	35.51763005016666	37.0	35.0	37.0	33.0	37.0
7	35.79184046323943	37.0	35.0	37.0	35.0	37.0
8	35.37316216022377	37.0	35.0	37.0	33.0	37.0
9	37.11786710554627	39.0	37.0	39.0	34.0	39.0
10	37.05111789730368	39.0	37.0	39.0	33.0	39.0
11	37.154467248232464	39.0	37.0	39.0	34.0	39.0
12	37.01277381217513	39.0	37.0	39.0	33.0	39.0
13	36.917762921971786	39.0	37.0	39.0	33.0	39.0
14	37.9191784596685	40.0	37.0	41.0	33.0	41.0
15	37.883208703103236	40.0	37.0	41.0	33.0	41.0
16	37.8826651366277	40.0	36.0	41.0	33.0	41.0
17	37.75820728756554	39.0	36.0	41.0	33.0	41.0
18	37.616615770222374	39.0	36.0	41.0	33.0	41.0
19	37.445943446437944	39.0	36.0	41.0	32.0	41.0
20	37.42670723283142	39.0	35.0	41.0	32.0	41.0
21	37.49831456644912	39.0	35.0	41.0	33.0	41.0
22	37.63228860360037	39.0	35.0	41.0	33.0	41.0
23	37.552225791474314	39.0	35.0	41.0	33.0	41.0
24	37.48430640540245	39.0	35.0	41.0	33.0	41.0
25	37.20077609213452	39.0	35.0	41.0	32.0	41.0
26	37.372924349890724	39.0	35.0	41.0	33.0	41.0
27	37.321825326422996	39.0	35.0	41.0	33.0	41.0
28	37.17309194955401	39.0	35.0	41.0	32.0	41.0
29	37.2248968544865	39.0	35.0	41.0	32.0	41.0
30	36.96226365238924	39.0	35.0	41.0	31.0	41.0
31	36.878833747928596	39.0	35.0	41.0	31.0	41.0
32	36.671130957998166	39.0	35.0	41.0	31.0	41.0
33	36.56204396093871	39.0	35.0	41.0	30.0	41.0
34	36.32171963294164	39.0	35.0	41.0	30.0	41.0
35	36.21524477477852	39.0	35.0	41.0	30.0	41.0
36	36.118346500979555	39.0	35.0	41.0	30.0	41.0
37	35.941045686007314	39.0	35.0	41.0	29.0	41.0
38	35.701212077745105	38.0	35.0	40.0	28.0	41.0
39	35.721225893393026	38.0	35.0	40.0	28.0	41.0
40	35.607941355216916	38.0	35.0	40.0	27.0	41.0
41	35.54825473638913	38.0	35.0	40.0	27.0	41.0
42	35.41860280767184	38.0	35.0	40.0	27.0	41.0
43	35.45071097740047	38.0	35.0	40.0	28.0	41.0
44	35.520740458332234	38.0	35.0	40.0	28.0	41.0
45	35.58027986123956	38.0	35.0	40.0	28.0	41.0
46	35.56616978147873	38.0	35.0	40.0	28.0	41.0
47	35.43869966819796	38.0	35.0	40.0	27.0	41.0
48	35.403284802409814	38.0	35.0	40.0	28.0	41.0
49	35.435241981450794	38.0	35.0	40.0	28.0	41.0
50	35.246028001223024	38.0	35.0	40.0	27.0	41.0
51	34.37627634315653	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	5.0
11	1.0
12	9.0
13	5.0
14	17.0
15	17.0
16	16.0
17	51.0
18	90.0
19	230.0
20	552.0
21	959.0
22	1394.0
23	1630.0
24	2071.0
25	2456.0
26	3023.0
27	3341.0
28	3597.0
29	3770.0
30	4532.0
31	5568.0
32	7201.0
33	9927.0
34	16979.0
35	22840.0
36	21746.0
37	32529.0
38	56082.0
39	64248.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.671406516003124	16.47648131301502	20.301075431172784	13.551036739809073
2	27.97177984047834	34.92829829720252	21.555053092100547	15.544868770218597
3	27.00241962576958	33.993288463933986	25.546491920110824	13.457799990185606
4	24.690374721139076	21.18474843064054	37.55251644854803	16.572360399672352
5	31.529120441496772	24.539006556770612	24.5533506720973	19.37852232963532
6	22.52516826024755	39.21454644284814	24.42047886696588	13.839806429938434
7	65.83722448917962	5.503233088099291	23.189904762623765	5.469637660097313
8	63.445154520094974	15.472015763427791	13.603128527048094	7.479701189429141
9	57.92531245635425	7.262652075933216	15.413506871963673	19.398528595748857
10	40.54515187775794	20.157634277905913	22.071441243861283	17.225772600474865
11	32.273882008327135	19.941717594567354	27.48785468656221	20.296545710543302
12	25.05803704556521	16.9611614203694	32.42223035894261	25.558571175122776
13	24.459358969035584	21.431995681666333	37.43851847937278	16.670126869925298
14	19.50384460038427	29.919559711154815	28.71352159355572	21.863074094905198
15	15.418791546031398	21.59506562432762	41.44732123646274	21.53882159317824
16	17.020047788552642	21.495034293759932	30.679797823469237	30.805120094218193
17	17.460185643050465	24.24797200632651	33.102443406802884	25.18939894382014
18	18.197397675498365	22.50402956397664	32.724211734241294	26.574361026283704
19	19.159208355824656	24.325354733746796	29.044568676226895	27.470868234201657
20	21.901199243536652	23.98185091934455	34.10653147967099	20.010418357447804
21	20.533978566871887	30.842112812692278	28.941895008625345	19.682013611810493
22	18.17890131626132	22.06728899995093	31.67407150164014	28.07973818214762
23	20.054583133585236	27.92459525058792	29.206128712011687	22.814692903815157
24	21.640362830622422	24.204939660346447	27.850232336920623	26.304465172110508
25	17.335995802458886	32.430912323482445	26.406383886273815	23.826707987784854
26	18.99878075019723	22.992484438522254	31.691812907438933	26.31692190384158
27	24.017333730942145	25.21431240728228	25.675966434770135	25.09238742700544
28	17.24804372690314	26.72270937689918	31.982092504444786	24.04715439175289
29	24.3302619310954	22.258669696546466	27.979706851579927	25.431361520778207
30	19.67597398430452	29.91012279317673	27.006571869679934	23.407331352838813
31	25.4038057202822	26.82274070746687	21.16021244389752	26.61324112835341
32	25.74202486061672	32.6581533083947	20.9431633304016	20.65665850058698
33	23.372603494679467	27.059418610357206	21.389340812405393	28.178637082557934
34	28.299052155958282	25.7593887896964	22.69201297010007	23.249546084245253
35	22.790911870510385	27.540701427239473	22.199783328363225	27.468603373886914
36	29.151017111019677	28.79052684425688	21.900821766817533	20.157634277905913
37	20.48226425635199	28.36511058180487	26.98430074325166	24.16832441859148
38	19.579717420928063	34.68407085992972	21.517305420188208	24.218906298954014
39	27.027710565950848	28.930193230332517	23.64627411604389	20.395822087672745
40	21.444452413397403	29.362781550447874	26.076091757040885	23.116674279113838
41	27.424816074468605	25.020289373652883	22.008025155048564	25.54686939682995
42	22.339827191157983	26.391662294228002	28.530445384780894	22.738065129833117
43	28.333402537398506	23.368828727488232	23.671942532944282	24.625826202168984
44	23.48735641729296	23.49226361464157	27.74302894868959	25.27735101937588
45	21.093021587893567	23.057032957492346	26.68005450763824	29.169890946975848
46	28.657277562406335	26.376940702182196	24.968952539852104	19.996829195559364
47	19.284153149854482	24.891192335712695	34.309991431278476	21.514663083154346
48	23.57153372565747	24.933469728254508	25.293205041579057	26.201791504508957
49	21.64149526077979	20.94580566743546	33.99215603377662	23.42054303800813
50	23.993930174356496	20.3773257284357	29.385807630314396	26.2429364668934
51	22.256782312950847	20.961659689638644	26.09911783690741	30.6824401605031
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	209.0
2	348.0
3	1921.0
4	3494.0
5	2288.5
6	1083.0
7	1020.0
8	957.0
9	1007.0
10	1057.0
11	1043.5
12	1030.0
13	991.5
14	953.0
15	892.5
16	832.0
17	818.5
18	805.0
19	732.0
20	659.0
21	683.5
22	708.0
23	709.0
24	710.0
25	1018.5
26	1408.0
27	1489.0
28	1826.0
29	2163.0
30	2319.5
31	2476.0
32	3000.0
33	3524.0
34	3578.5
35	3633.0
36	4413.5
37	5194.0
38	5254.5
39	5315.0
40	6538.0
41	7761.0
42	9680.5
43	11600.0
44	13650.5
45	15701.0
46	31871.0
47	48041.0
48	39093.5
49	30146.0
50	28792.0
51	27438.0
52	22954.5
53	18471.0
54	15854.0
55	13237.0
56	12128.0
57	11019.0
58	10113.5
59	9208.0
60	8589.0
61	7970.0
62	7283.0
63	6596.0
64	6166.5
65	5737.0
66	4981.0
67	4225.0
68	3546.0
69	2867.0
70	2570.5
71	2274.0
72	2111.0
73	1948.0
74	1546.5
75	931.0
76	717.0
77	620.0
78	523.0
79	382.0
80	241.0
81	166.5
82	92.0
83	77.5
84	63.0
85	62.5
86	62.0
87	35.0
88	8.0
89	4.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	264917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.70634198635799
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.51635252275867	26.208963562172304
2	9.37102335142425	6.504678823933534
3	3.3836181112210824	3.5229902195782077
4	1.7608735847209684	2.444539233042802
5	1.2029191999390927	2.0874462567521146
6	0.9157847797004666	1.9070123850111544
7	0.7646041569233112	1.8575629348059959
8	0.7428515493294758	2.0625327932899737
9	0.5710059493381769	1.7835774978578196
>10	5.638275888322113	34.121630548435924
>50	0.08265990885657418	1.9696735203856304
>100	0.035891802529828265	2.4030167939392335
>500	0.003262891139075297	0.7172057663343614
>1k	0.0076134126578423595	4.498390061792938
>5k	0.003262891139075297	7.910779602668006
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGC	7461	2.8163538013793	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCG	7210	2.7216071448793397	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTC	6286	2.372818656409366	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	2976	1.123370716111084	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2399	0.9055666491769121	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCT	1663	0.6277437839021278	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAAGAGT	1439	0.5431889988184979	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	1191	0.44957477247590755	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTA	1181	0.4458000052846741	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTC	1068	0.40314513602373575	No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT	734	0.27706791183653745	No Hit
CCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	589	0.222333787563652	No Hit
TCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	577	0.21780406693417184	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	441	0.16646723313339648	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCC	436	0.16457984953777977	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATG	425	0.16042760562742295	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTT	420	0.15854022203180618	No Hit
ACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	355	0.13400423528878858	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAAAGAG	306	0.11550787605174452	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTAT	301	0.11362049245612776	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGT	280	0.10569348135453746	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7747671912334807E-4	0.0	0.0	0.2932994107588415	0.0
2	3.7747671912334807E-4	0.0	0.0	2.091221023943348	0.0
3	3.7747671912334807E-4	0.0	0.0	2.569106550353507	0.0
4	3.7747671912334807E-4	0.0	0.0	3.6732259537893	0.0
5	3.7747671912334807E-4	0.0	0.0	7.611063087684067	0.0
6	3.7747671912334807E-4	0.0	0.0	8.723109502221451	0.0
7	3.7747671912334807E-4	0.0	0.0	10.34210715054149	0.0
8	3.7747671912334807E-4	0.0	0.0	12.386521061313543	0.0
9	3.7747671912334807E-4	0.0	0.0	13.082210654657874	0.0
10	3.7747671912334807E-4	0.0	0.0	17.213315868743795	0.0
11	3.7747671912334807E-4	0.0	0.0	18.917245778866587	0.0
12	3.7747671912334807E-4	0.0	0.0	23.29182347678707	0.0
13	3.7747671912334807E-4	0.0	0.0	24.088299354137334	0.0
14	3.7747671912334807E-4	0.0	0.0	24.436332889169062	0.0
15	3.7747671912334807E-4	0.0	0.0	25.298489715646788	0.0
16	3.7747671912334807E-4	0.0	0.0	26.224062630937237	0.0
17	3.7747671912334807E-4	0.0	0.0	27.299493803719656	0.0
18	3.7747671912334807E-4	0.0	0.0	28.382474510884542	0.0
19	3.7747671912334807E-4	0.0	0.0	29.606254034282436	0.0
20	3.7747671912334807E-4	0.0	0.0	30.30232110434589	0.0
21	3.7747671912334807E-4	0.0	0.0	30.963660316249996	0.0
22	3.7747671912334807E-4	0.0	0.0	31.644628317548516	0.0
23	3.7747671912334807E-4	0.0	0.0	32.26180275331519	0.0
24	3.7747671912334807E-4	0.0	0.0	32.760072022558006	0.0
25	3.7747671912334807E-4	0.0	0.0	33.201719783932326	0.0
26	3.7747671912334807E-4	0.0	0.0	33.64902969609349	0.0
27	3.7747671912334807E-4	0.0	0.0	34.125405315627155	0.0
28	3.7747671912334807E-4	0.0	0.0	34.52666306805528	0.0
29	3.7747671912334807E-4	0.0	0.0	34.947927086596934	0.0
30	3.7747671912334807E-4	0.0	0.0	35.3790054998358	0.0
31	3.7747671912334807E-4	0.0	0.0	35.837262236851544	0.0
32	3.7747671912334807E-4	0.0	0.0	36.26683074321391	0.0
33	3.7747671912334807E-4	0.0	0.0	36.63109577716794	0.0
34	3.7747671912334807E-4	0.0	0.0	37.037260726944666	0.0
35	7.549534382466961E-4	0.0	0.0	37.46154455923931	0.0
36	7.549534382466961E-4	0.0	0.0	37.82807445350808	0.0
37	7.549534382466961E-4	0.0	0.0	38.19422687105773	0.0
38	7.549534382466961E-4	0.0	0.0	38.589822472699	0.0
39	7.549534382466961E-4	0.0	0.0	38.94616049555144	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.023449E-4	45.0	25
TTGATCC	20	7.023449E-4	45.0	17
CAGCGGA	50	2.1827873E-11	45.0	37
AACCGCG	20	7.023449E-4	45.0	1
TGATAAG	20	7.023449E-4	45.0	26
TACCCTT	20	7.023449E-4	45.0	23
CTCACGA	20	7.023449E-4	45.0	24
TACCCGC	20	7.023449E-4	45.0	28
CGTATGG	25	3.8826536E-5	45.0	2
GTCGAAT	70	0.0	45.0	43
CAAGTCG	20	7.023449E-4	45.0	15
GGTCGAA	20	7.023449E-4	45.0	42
ATTACGG	20	7.023449E-4	45.0	2
TATATCA	25	3.8826536E-5	45.0	41
GTTACGG	20	7.023449E-4	45.0	2
TGTCTAT	25	3.8826536E-5	45.0	37
CGAGGGT	25	3.8826536E-5	45.0	4
GCTTAAG	20	7.023449E-4	45.0	1
GCGGGTT	45	3.8198777E-10	45.0	5
TCTAAAC	20	7.023449E-4	45.0	34
>>END_MODULE