FastQCFastQC Report
Sat 18 Jun 2016
SRR3551625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480128
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC108552.26085543854972No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG94521.9686416955478538No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC85991.7909807384697412No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49931.0399310183950947No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC32200.6706544921354305No Hit
GAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT27240.5673487070114636No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT20020.4169721407624633No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTC15120.31491602239402827No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTA14380.29950346574246867No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCGAAAAT14070.29304685417222076No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT10590.22056618235137296No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT6020.1253832311383631No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC5460.11371967475339909No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4880.10163956278325781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA551.8189894E-1245.05
TAGTCCA207.029179E-445.016
CGAACCC302.1628512E-644.99999634
CACAACG600.044.99999612
CCGATGA13850.042.5631818
CGAATGC1700.042.3529445
CGTTTTT25400.042.1653561
TACGGCT10050.042.0895547
GATGAAT15250.042.04918320
ATTAGTC750.042.031
TTAGTCA750.042.032
ATGAATG15450.041.35922221
CGATGAA14750.041.33898519
TGAATGA15300.041.1764722
ACGGCTG10700.039.532718
GCGAACC403.4526238E-739.37533
CAAATCG403.4526238E-739.37540
TAGTGCG403.4526238E-739.3751
ACCGATG15100.038.89072417
TCGAATA356.2406925E-638.57142644
Produced by FastQC (version 0.11.5)