##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551625_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 480128 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87254857038123 33.0 31.0 34.0 30.0 34.0 2 31.96799186883498 33.0 31.0 34.0 30.0 34.0 3 31.937325046654227 33.0 31.0 34.0 30.0 34.0 4 35.62643295121301 37.0 35.0 37.0 33.0 37.0 5 35.63178152492669 37.0 35.0 37.0 33.0 37.0 6 35.50922254065583 37.0 35.0 37.0 33.0 37.0 7 35.79691665555852 37.0 35.0 37.0 35.0 37.0 8 35.439561950146626 37.0 35.0 37.0 33.0 37.0 9 37.191471857504666 39.0 37.0 39.0 34.0 39.0 10 36.939164139562784 39.0 37.0 39.0 33.0 39.0 11 37.06423703679019 39.0 37.0 39.0 34.0 39.0 12 36.9819131564916 39.0 37.0 39.0 33.0 39.0 13 37.00308875966409 39.0 37.0 39.0 33.0 39.0 14 38.08115335910424 40.0 37.0 41.0 33.0 41.0 15 38.062212576646225 40.0 37.0 41.0 33.0 41.0 16 37.95838401426286 40.0 37.0 41.0 33.0 41.0 17 37.81470149626766 40.0 36.0 41.0 33.0 41.0 18 37.65878682351373 39.0 36.0 41.0 33.0 41.0 19 37.44861370301253 39.0 36.0 41.0 32.0 41.0 20 37.44009514129566 39.0 35.0 41.0 32.0 41.0 21 37.509430818448415 39.0 35.0 41.0 33.0 41.0 22 37.627849240202615 39.0 35.0 41.0 33.0 41.0 23 37.54273860303919 40.0 35.0 41.0 33.0 41.0 24 37.47131181684884 39.0 35.0 41.0 33.0 41.0 25 37.28665064316182 39.0 35.0 41.0 32.0 41.0 26 37.357706694881365 39.0 35.0 41.0 33.0 41.0 27 37.349077745934416 39.0 35.0 41.0 33.0 41.0 28 37.18683351106372 39.0 35.0 41.0 32.0 41.0 29 37.21403042521994 39.0 35.0 41.0 32.0 41.0 30 36.9458998433751 39.0 35.0 41.0 31.0 41.0 31 36.913364769394825 39.0 35.0 41.0 31.0 41.0 32 36.69465850773127 39.0 35.0 41.0 31.0 41.0 33 36.42650293255132 39.0 35.0 41.0 30.0 41.0 34 36.12722648960277 39.0 35.0 41.0 29.0 41.0 35 36.038406424953344 39.0 35.0 41.0 28.0 41.0 36 35.870086726872835 39.0 35.0 41.0 27.0 41.0 37 35.73360853772327 39.0 35.0 41.0 27.0 41.0 38 35.633928868968276 39.0 35.0 41.0 26.0 41.0 39 35.3365810783791 39.0 35.0 40.0 26.0 41.0 40 35.180626832844574 38.0 35.0 40.0 25.0 41.0 41 34.99357254732072 38.0 34.0 40.0 24.0 41.0 42 34.88926494601439 38.0 35.0 40.0 24.0 41.0 43 34.816434367502 37.0 34.0 40.0 24.0 41.0 44 34.89110195614503 37.0 34.0 40.0 26.0 41.0 45 34.77579312183418 37.0 34.0 40.0 26.0 41.0 46 34.64683376099707 37.0 34.0 40.0 25.0 41.0 47 34.43672520661157 36.0 34.0 40.0 24.0 41.0 48 34.41278575713143 36.0 34.0 40.0 25.0 41.0 49 34.37646211010397 36.0 34.0 40.0 26.0 41.0 50 34.20222940549187 35.0 34.0 40.0 25.0 41.0 51 33.36811850173287 35.0 33.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 9.0 10 12.0 11 10.0 12 16.0 13 20.0 14 24.0 15 31.0 16 53.0 17 82.0 18 212.0 19 426.0 20 1079.0 21 2171.0 22 3238.0 23 3862.0 24 4535.0 25 5389.0 26 6501.0 27 7269.0 28 7221.0 29 7608.0 30 8575.0 31 10307.0 32 13061.0 33 17474.0 34 29772.0 35 37670.0 36 41294.0 37 64609.0 38 95223.0 39 112316.0 40 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.98996934150893 16.670971074380166 19.977172753932283 16.36188683017862 2 29.785807118101836 33.2838326446281 21.619026592908558 15.311333644361502 3 27.81341642228739 32.37990702479338 25.933084510797123 13.873592042122102 4 24.828587376699545 21.667971874166888 36.92036290322581 16.583077845907756 5 30.979863703012526 25.28783990935751 24.93043521727539 18.801861170354574 6 23.16902992535324 37.96196014396161 25.41905491868835 13.449955011996801 7 69.18738336443614 5.611836843508398 20.839234537456676 4.361545254598774 8 68.62128432418022 13.081511596907491 12.813458077845908 5.4837460010663825 9 63.22522327379365 6.431409957344708 13.972732271394294 16.370634497467343 10 40.43754998667023 20.822364036257 22.41089876033058 16.329187216742202 11 32.46946647560651 20.783416089042923 27.20899426819515 19.538123167155426 12 24.39057917888563 18.82248067182085 31.448905291922152 25.338034857371365 13 25.54068914956012 20.005915089309518 35.61904325513196 18.8343525059984 14 20.71614236203679 27.627216075713147 29.72436516928819 21.932276392961878 15 19.062000133297786 21.07812916555585 36.901826179685415 22.958044521460945 16 21.448863636363637 22.41069048253799 28.652359370834446 27.488086510263933 17 20.82507164756065 25.230980071980806 31.345807784590775 22.598140495867767 18 21.432201412956545 22.31550753132498 30.366277326046387 25.886013729672086 19 21.691298986936815 24.334552452679286 27.378324113569715 26.59582444681418 20 25.826862836576915 23.550386563583046 31.313108171154358 19.309642428685684 21 22.435892095441215 29.957636296987474 28.356188349773394 19.25028325779792 22 20.704478805651828 21.016270661157023 32.63254798720342 25.646702545987736 23 24.125858104505465 25.567556984804053 29.243243468408426 21.06334144228206 24 23.21964142895228 24.963134830711812 27.695947747267397 24.121275993068515 25 21.005856771527593 30.177785923753664 26.383589376166356 22.432767928552387 26 21.01106371634231 22.374658424420154 31.448697014129568 25.16558084510797 27 26.935942082111435 22.96345974406825 26.365052652625963 23.73554552119435 28 20.416014062916553 25.842900226606236 30.14342008797654 23.597665622500667 29 27.45142961876833 20.95545354572114 28.67277059450813 22.9203462410024 30 24.80338576379632 27.173170487869903 27.28272460677153 20.74071914156225 31 25.50507364702746 22.381739869368168 23.896127699280193 28.217058784324177 32 30.912589976006398 24.964384497467343 23.663689682751265 20.45933584377499 33 25.901842841908824 25.986611903492403 23.08092841908824 25.030616835510532 34 25.346157691282322 26.309442482004798 24.580736803519063 23.763663023193814 35 26.988219808051184 26.756614902692615 23.75220774460144 22.50295754465476 36 23.50081644894695 30.99944181551586 23.097798920287925 22.401942815249267 37 25.838734670754466 25.997650626499603 26.12407524660091 22.039539456145025 38 21.32702112769928 28.973523727006135 22.713318115169287 26.986137030125303 39 25.976198013862973 28.550719808051184 24.40786623567049 21.065215942415357 40 22.012046787523325 28.21414289522794 26.804727072780594 22.96908324446814 41 20.072355705145295 30.585385563849638 24.38120667821914 24.961052052785924 42 22.400276592908558 26.503557384697412 28.397427352705947 22.698738669688083 43 24.604688749666757 25.902259397494003 25.07977039456145 24.41328145827779 44 24.523460410557185 22.598765329245534 27.61222007464676 25.26555418555052 45 22.37549153559051 22.269478139162892 27.251899493468407 28.103130831778195 46 27.155258597707277 25.891428952279394 25.629207211410293 21.32410523860304 47 20.316457278059183 25.599423487070116 32.441348973607035 21.642770261263664 48 22.412981538256467 25.82082278059184 26.784107571314316 24.982088109837377 49 22.32821247667289 22.615635830445218 31.90211776859504 23.154033924286857 50 22.369034924020262 22.829537123433752 29.946181018395094 24.855246934150895 51 22.437558317781924 22.642920221274327 27.02591808850973 27.89360337243402 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 119.0 1 358.0 2 597.0 3 4386.0 4 8175.0 5 5318.5 6 2462.0 7 2382.0 8 2302.0 9 2191.0 10 2080.0 11 2034.0 12 1988.0 13 1964.5 14 1941.0 15 1823.5 16 1706.0 17 1646.5 18 1587.0 19 1511.0 20 1435.0 21 1534.0 22 1633.0 23 1616.5 24 1600.0 25 1951.0 26 2577.5 27 2853.0 28 3453.0 29 4053.0 30 4670.5 31 5288.0 32 5813.0 33 6338.0 34 7598.5 35 8859.0 36 9615.0 37 10371.0 38 11015.5 39 11660.0 40 12984.0 41 14308.0 42 15966.0 43 17624.0 44 20008.0 45 22392.0 46 45158.5 47 67925.0 48 54417.0 49 40909.0 50 41050.0 51 41191.0 52 37403.5 53 33616.0 54 31412.5 55 29209.0 56 26944.5 57 24680.0 58 23897.5 59 23115.0 60 22192.5 61 21270.0 62 19635.5 63 18001.0 64 16302.0 65 14603.0 66 12634.5 67 10666.0 68 8870.0 69 7074.0 70 6100.5 71 5127.0 72 4337.5 73 3548.0 74 3077.5 75 1941.5 76 1276.0 77 1083.5 78 891.0 79 673.5 80 456.0 81 311.0 82 166.0 83 121.0 84 76.0 85 51.5 86 27.0 87 17.0 88 7.0 89 7.5 90 8.0 91 5.0 92 2.0 93 2.0 94 2.0 95 2.5 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 480128.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.0910051617557 #Duplication Level Percentage of deduplicated Percentage of total 1 72.32613268554894 26.103228280839748 2 7.748428268116317 5.592971292401596 3 3.751219740083128 4.0615587300666025 4 2.5619904065382055 3.698592359469558 5 2.1280340632907797 3.8401444181309743 6 1.7961522063338755 3.8894963130056874 7 1.5405528496068805 3.8920070592983564 8 1.3621024336955694 3.9327716772277435 9 1.23327503061643 4.005912194625778 >10 5.473329074431815 27.80055120498953 >50 0.04112948649563282 1.0079315689980388 >100 0.030123004193984556 2.053914070490919 >500 0.0011585770843840213 0.24001357419824668 >1k 0.004634308337536085 3.837499263422315 >5k 0.0011585770843840213 3.773941661892465 >10k+ 5.792885421920106E-4 2.26946633094248 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC 10855 2.26085543854972 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG 9452 1.9686416955478538 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC 8599 1.7909807384697412 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4993 1.0399310183950947 No Hit GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC 3220 0.6706544921354305 No Hit GAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT 2724 0.5673487070114636 No Hit GAATGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT 2002 0.4169721407624633 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTC 1512 0.31491602239402827 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTA 1438 0.29950346574246867 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCGAAAAT 1407 0.29304685417222076 No Hit GAATGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT 1059 0.22056618235137296 No Hit CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT 602 0.1253832311383631 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC 546 0.11371967475339909 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC 488 0.10163956278325781 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.0827779258864303E-4 0.0 0.0 0.1316315649160224 0.0 2 2.0827779258864303E-4 0.0 0.0 1.0063982937883231 0.0 3 2.0827779258864303E-4 0.0 0.0 1.2594558117835244 0.0 4 2.0827779258864303E-4 0.0 0.0 2.1217258731005066 0.0 5 2.0827779258864303E-4 0.0 0.0 5.158624366835511 0.0 6 2.0827779258864303E-4 0.0 0.0 6.1087876566249 0.0 7 2.0827779258864303E-4 0.0 0.0 7.13559717408691 0.0 8 2.0827779258864303E-4 0.0 0.0 8.404217208744335 0.0 9 2.0827779258864303E-4 0.0 0.0 8.899918355105305 0.0 10 2.0827779258864303E-4 0.0 0.0 11.717708611037057 0.0 11 2.0827779258864303E-4 0.0 0.0 12.660373900293255 0.0 12 2.0827779258864303E-4 0.0 0.0 15.266762196747534 0.0 13 2.0827779258864303E-4 0.0 0.0 15.704145561183685 0.0 14 2.0827779258864303E-4 0.0 0.0 15.925336576912823 0.0 15 2.0827779258864303E-4 0.0 0.0 16.458736003732337 0.0 16 2.0827779258864303E-4 0.0 0.0 16.91861336976806 0.0 17 2.0827779258864303E-4 0.0 0.0 17.42243735003999 0.0 18 2.0827779258864303E-4 0.0 0.0 17.974373500399892 0.0 19 2.0827779258864303E-4 0.0 0.0 18.64232038123167 0.0 20 2.0827779258864303E-4 0.0 0.0 19.020969408157825 0.0 21 2.0827779258864303E-4 0.0 0.0 19.38066515595841 0.0 22 2.0827779258864303E-4 0.0 0.0 19.78118335110637 0.0 23 2.0827779258864303E-4 0.0 0.0 20.168996600906425 0.0 24 2.0827779258864303E-4 0.0 0.0 20.484745734470806 0.0 25 2.0827779258864303E-4 0.0 0.0 20.76821181018395 0.0 26 2.0827779258864303E-4 0.0 0.0 21.02626799520128 0.0 27 2.0827779258864303E-4 0.0 0.0 21.329312183417755 0.0 28 2.0827779258864303E-4 0.0 0.0 21.60153125833111 0.0 29 2.0827779258864303E-4 0.0 0.0 21.887913223140497 0.0 30 2.0827779258864303E-4 0.0 0.0 22.200954745401226 0.0 31 2.0827779258864303E-4 0.0 0.0 22.47942215409224 0.0 32 2.0827779258864303E-4 0.0 0.0 22.7589309517462 0.0 33 2.0827779258864303E-4 0.0 0.0 23.039689416155692 0.0 34 2.0827779258864303E-4 0.0 0.0 23.330445214609437 0.0 35 2.0827779258864303E-4 0.0 0.0 23.61682717941882 0.0 36 2.0827779258864303E-4 0.0 0.0 23.90716642228739 0.0 37 2.0827779258864303E-4 0.0 0.0 24.201046387629965 0.0 38 2.0827779258864303E-4 0.0 0.0 24.490969074913355 0.0 39 4.1655558517728606E-4 0.0 0.0 24.817965209277524 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGGTA 55 1.8189894E-12 45.0 5 TAGTCCA 20 7.029179E-4 45.0 16 CGAACCC 30 2.1628512E-6 44.999996 34 CACAACG 60 0.0 44.999996 12 CCGATGA 1385 0.0 42.56318 18 CGAATGC 170 0.0 42.35294 45 CGTTTTT 2540 0.0 42.165356 1 TACGGCT 1005 0.0 42.089554 7 GATGAAT 1525 0.0 42.049183 20 ATTAGTC 75 0.0 42.0 31 TTAGTCA 75 0.0 42.0 32 ATGAATG 1545 0.0 41.359222 21 CGATGAA 1475 0.0 41.338985 19 TGAATGA 1530 0.0 41.17647 22 ACGGCTG 1070 0.0 39.53271 8 GCGAACC 40 3.4526238E-7 39.375 33 CAAATCG 40 3.4526238E-7 39.375 40 TAGTGCG 40 3.4526238E-7 39.375 1 ACCGATG 1510 0.0 38.890724 17 TCGAATA 35 6.2406925E-6 38.571426 44 >>END_MODULE