SRR3551624_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551624_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	213322
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG	5514	2.5848248188184995	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC	5335	2.50091411106215	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC	5219	2.4465362222368063	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	2736	1.282568136432248	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1902	0.8916098667741724	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCT	1413	0.6623789388811281	No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT	1018	0.4772128519327589	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTC	969	0.4542428816530878	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTA	935	0.4383045349284181	No Hit
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT	908	0.42564761252941563	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	878	0.41158436541941285	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTGGAT	857	0.401740092442411	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	615	0.28829656575505574	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCC	465	0.21798033020504212	No Hit
ACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	360	0.16875896532003262	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTT	330	0.15469571821002992	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATG	294	0.13781982167802664	No Hit
GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT	231	0.10828700274702094	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGATTGGATCGT	221	0.10359925371035336	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	214	0.10031782938468607	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGGGT	35	1.2057171E-7	45.000004	4
AATGCGG	45	3.8198777E-10	45.000004	2
CGCTAGG	35	1.2057171E-7	45.000004	2
ATCCTTG	20	7.0203585E-4	45.0	29
TTCTAGG	20	7.0203585E-4	45.0	2
AGCGTCG	20	7.0203585E-4	45.0	17
CCTTAAC	25	3.8800932E-5	45.0	34
GTATTTA	20	7.0203585E-4	45.0	41
ACGCCCT	20	7.0203585E-4	45.0	39
GAATGCG	20	7.0203585E-4	45.0	1
TAAAGCG	20	7.0203585E-4	45.0	1
CTATATA	25	3.8800932E-5	45.0	40
ATCAGAC	25	3.8800932E-5	45.0	21
TGCGGGC	20	7.0203585E-4	45.0	4
TATGCGG	25	3.8800932E-5	45.0	2
CGTTCGG	25	3.8800932E-5	45.0	45
ACGGGAC	20	7.0203585E-4	45.0	5
CGCCTTA	25	3.8800932E-5	45.0	32
TATTGCT	20	7.0203585E-4	45.0	22
TAACCCG	25	3.8800932E-5	45.0	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content