##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551624_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	213322
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.805036517564997	33.0	31.0	34.0	30.0	34.0
2	31.881493704353044	33.0	31.0	34.0	30.0	34.0
3	31.878695118178154	33.0	31.0	34.0	30.0	34.0
4	35.582115299875305	37.0	35.0	37.0	33.0	37.0
5	35.61775625580109	37.0	35.0	37.0	33.0	37.0
6	35.51155530137539	37.0	35.0	37.0	33.0	37.0
7	35.81051180843982	37.0	35.0	37.0	35.0	37.0
8	35.44065309719579	37.0	35.0	37.0	33.0	37.0
9	37.20601719466347	39.0	37.0	39.0	34.0	39.0
10	37.04719625730117	39.0	37.0	39.0	33.0	39.0
11	37.153439401468205	39.0	37.0	39.0	34.0	39.0
12	37.019027573339834	39.0	37.0	39.0	33.0	39.0
13	36.9020072941375	39.0	37.0	39.0	33.0	39.0
14	37.835755337002276	40.0	37.0	41.0	33.0	41.0
15	37.86472093829985	40.0	36.0	41.0	33.0	41.0
16	37.80136601006929	39.0	36.0	41.0	33.0	41.0
17	37.76224674435829	39.0	36.0	41.0	33.0	41.0
18	37.61995012235025	39.0	36.0	41.0	33.0	41.0
19	37.42630858514359	39.0	36.0	41.0	32.0	41.0
20	37.512000637533866	39.0	35.0	41.0	33.0	41.0
21	37.500173446714356	39.0	35.0	41.0	33.0	41.0
22	37.643131041336574	39.0	35.0	41.0	33.0	41.0
23	37.61516861833285	39.0	35.0	41.0	33.0	41.0
24	37.481244316104295	39.0	35.0	41.0	33.0	41.0
25	37.22367594528459	39.0	35.0	41.0	33.0	41.0
26	37.42091767375142	39.0	35.0	41.0	33.0	41.0
27	37.434015244559866	39.0	35.0	41.0	33.0	41.0
28	37.248169434001184	39.0	35.0	41.0	33.0	41.0
29	37.40310422741208	39.0	35.0	41.0	33.0	41.0
30	37.15024704437423	39.0	35.0	41.0	32.0	41.0
31	36.95927283636943	39.0	35.0	41.0	32.0	41.0
32	36.86983058474981	39.0	35.0	41.0	32.0	41.0
33	36.70409521755843	39.0	35.0	41.0	31.0	41.0
34	36.58025426350775	39.0	35.0	41.0	31.0	41.0
35	36.573255454196	39.0	35.0	41.0	31.0	41.0
36	36.35068581768407	39.0	35.0	41.0	30.0	41.0
37	36.292824931324475	39.0	35.0	41.0	30.0	41.0
38	36.08019801051931	39.0	35.0	40.0	30.0	41.0
39	36.03332989565071	39.0	35.0	40.0	30.0	41.0
40	35.97856292365532	39.0	35.0	40.0	30.0	41.0
41	35.706945368972725	38.0	35.0	40.0	28.0	41.0
42	35.77729910651503	38.0	35.0	40.0	29.0	41.0
43	35.759434094936296	38.0	35.0	40.0	29.0	41.0
44	35.84477925389786	38.0	35.0	40.0	29.0	41.0
45	35.78359475347128	38.0	35.0	40.0	29.0	41.0
46	35.61898913379773	38.0	35.0	40.0	28.0	41.0
47	35.357956516439934	38.0	35.0	40.0	27.0	41.0
48	35.35876749702328	38.0	35.0	40.0	28.0	41.0
49	35.41122809649263	38.0	35.0	40.0	28.0	41.0
50	35.25437601372573	38.0	35.0	40.0	27.0	41.0
51	34.15102989846336	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	6.0
10	4.0
11	1.0
12	5.0
13	6.0
14	4.0
15	11.0
16	24.0
17	21.0
18	53.0
19	151.0
20	304.0
21	522.0
22	819.0
23	1141.0
24	1552.0
25	1830.0
26	2398.0
27	2656.0
28	2721.0
29	3009.0
30	3534.0
31	4425.0
32	5687.0
33	8009.0
34	14233.0
35	20085.0
36	16931.0
37	25448.0
38	46042.0
39	51676.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.0837513242891	18.661928914973608	20.3415493948116	13.91277036592569
2	28.67261698277721	34.14743908270127	21.686464593431527	15.493479341089994
3	26.273895800714413	32.52547791601429	27.171599741236253	14.029026542035044
4	23.93236515689896	21.658338099211523	37.63840579030761	16.77089095358191
5	30.4989640074629	24.221130497557684	25.73105446226831	19.548851032711113
6	21.74974920542654	37.81607147879731	26.027320201385702	14.406859114390452
7	66.54212880059252	5.108240125256654	22.208679836116293	6.14095123803452
8	62.61192000825044	13.073663288362194	15.478478544172658	8.835938159214708
9	57.91292037389486	6.247363141166875	17.294512520977676	18.545203963960585
10	38.7611216845895	20.430616626508282	23.65906938806124	17.149192300840983
11	31.30150664254038	20.729695015047675	28.05945940878109	19.90933893363085
12	26.507814477644125	16.49009478628552	33.60881671838816	23.393274017682188
13	23.73688602206992	22.05867186694293	38.3012535040924	15.90318860689474
14	17.88094992546479	30.6963182419066	29.267961110433994	22.154770722194616
15	13.999962498007706	23.312644734251506	43.8023269986218	18.885065769118984
16	14.866258519983875	24.03408931099465	33.32520790166978	27.774444267351704
17	15.614892041139687	24.40489025979505	33.74569899025886	26.234518708806405
18	17.822353062506448	22.399939996812332	34.66309147673471	25.11461546394652
19	17.979861430138477	25.773712978501983	29.21170812199398	27.03471746936556
20	21.481609960529152	25.710897141410637	33.32614545147711	19.4813474465831
21	19.268054865414726	31.184312916623696	29.791113902926092	19.756518315035486
22	16.317585621736157	23.55828278377289	31.032898622739335	29.091232971751623
23	18.162214867664844	29.556257676189045	29.68001425075707	22.601513205389036
24	21.455827340827483	23.207639155830154	28.621051743373865	26.715481759968497
25	16.425872624483176	31.260254451017712	27.69522130863202	24.618651615867094
26	16.928399321213938	23.791263910895267	31.837785132335156	27.44255163555564
27	24.18784747939734	26.43046661853911	26.227018310347738	23.15466759171581
28	15.6866146014007	26.609069856836143	32.91268598644303	24.791629555320128
29	20.62187678720432	19.939809302369188	30.953675664019652	28.484638246406842
30	23.98721182062797	26.66157264604682	29.140454336636633	20.210761196688573
31	24.23191232034202	26.22514321073307	23.344052652797178	26.198891816127734
32	26.78486044571118	25.80605844685499	26.318429416562754	21.090651690871077
33	24.270351862442695	23.7851698371476	26.96487000871921	24.979608291690496
34	26.364369357122097	22.238681429950965	26.27764599994375	25.11930321298319
35	27.712097205164028	20.98611488735339	26.64844694874415	24.653340958738433
36	24.07112252838432	28.960913548532265	25.235559389092543	21.732404533990866
37	25.266967307638215	24.045339908682646	32.30890391052025	18.378788873158886
38	21.516299303400494	27.95539138016707	27.231602928905595	23.296706387526836
39	26.105136835394383	23.66516346180891	30.66116012413159	19.568539578665117
40	27.30098161464828	19.458846251207095	32.01216939649919	21.228002737645436
41	24.2984783566627	26.87017747817853	23.119509473940802	25.711834691217973
42	25.64386233018629	22.510102099174016	29.785019829178427	22.061015741461265
43	24.927574277383485	24.85303906770047	25.99544350793636	24.223943146979686
44	22.54572899185269	22.112580980864607	30.522402752646233	24.819287274636466
45	21.94147814102624	20.377176287490272	28.549329183112853	29.13201638837063
46	28.297597059843802	23.67360141007491	27.183787888731587	20.845013641349695
47	19.12695361941103	22.2452442786023	36.84898885253279	21.778813249453876
48	23.389992593356524	22.686830237856388	27.948828531515733	25.974348637271355
49	22.215242684767627	17.377016904023026	36.57006778485107	23.837672626358277
50	22.04882759396593	18.601925727304263	32.20014813286956	27.14909854586025
51	20.486869614948294	18.91178593862799	27.440676535940973	33.16066791048274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57.0
1	130.0
2	203.0
3	1497.0
4	2791.0
5	1758.5
6	726.0
7	762.0
8	798.0
9	832.0
10	866.0
11	854.5
12	843.0
13	848.5
14	854.0
15	838.0
16	822.0
17	736.5
18	651.0
19	633.5
20	616.0
21	628.5
22	641.0
23	688.0
24	735.0
25	804.0
26	1007.0
27	1141.0
28	1262.0
29	1383.0
30	1511.0
31	1639.0
32	1983.5
33	2328.0
34	2779.0
35	3230.0
36	3367.0
37	3504.0
38	3970.0
39	4436.0
40	5648.0
41	6860.0
42	8191.0
43	9522.0
44	12019.5
45	14517.0
46	27778.5
47	41040.0
48	34258.0
49	27476.0
50	26574.5
51	25673.0
52	20377.5
53	15082.0
54	12536.5
55	9991.0
56	8630.5
57	7270.0
58	6394.5
59	5519.0
60	5631.0
61	5743.0
62	5360.0
63	4977.0
64	4257.5
65	3538.0
66	2904.5
67	2271.0
68	1904.0
69	1537.0
70	1345.5
71	1154.0
72	953.0
73	752.0
74	662.5
75	447.5
76	322.0
77	243.5
78	165.0
79	127.5
80	90.0
81	79.5
82	69.0
83	49.5
84	30.0
85	16.0
86	2.0
87	3.5
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	213322.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.38443292299903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.45222634508349	27.452864683436307
2	10.023706452277882	7.2941375010547445
3	3.6448670377241803	3.9784926074197693
4	1.8488455988455987	2.690767947047187
5	1.2226860441146155	2.2243369178987633
6	0.8915687487116057	1.9463534000243765
7	0.7279426922284066	1.854004744002025
8	0.6210059781488353	1.8075960285390162
9	0.5385487528344671	1.763531187594341
>10	4.88301381158524	30.949456689886652
>50	0.08761080189651618	2.1005803433307393
>100	0.04122861265718408	2.6720169509005167
>500	0.007730364873222016	2.419816052727801
>1k	0.00515357658214801	3.3137697940203075
>5k	0.003865182436611008	7.532275152117457
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG	5514	2.5848248188184995	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC	5335	2.50091411106215	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC	5219	2.4465362222368063	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	2736	1.282568136432248	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1902	0.8916098667741724	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCT	1413	0.6623789388811281	No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT	1018	0.4772128519327589	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTC	969	0.4542428816530878	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTA	935	0.4383045349284181	No Hit
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT	908	0.42564761252941563	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	878	0.41158436541941285	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTGGAT	857	0.401740092442411	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	615	0.28829656575505574	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCC	465	0.21798033020504212	No Hit
ACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	360	0.16875896532003262	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTT	330	0.15469571821002992	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATG	294	0.13781982167802664	No Hit
GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT	231	0.10828700274702094	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGATTGGATCGT	221	0.10359925371035336	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	214	0.10031782938468607	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.687749036667573E-4	0.0	0.0	0.4448673835797527	0.0
2	4.687749036667573E-4	0.0	0.0	2.6917054968545204	0.0
3	4.687749036667573E-4	0.0	0.0	3.403305800620658	0.0
4	4.687749036667573E-4	0.0	0.0	4.86588350006094	0.0
5	4.687749036667573E-4	0.0	0.0	9.790363863080227	0.0
6	4.687749036667573E-4	0.0	0.0	11.396386683042536	0.0
7	4.687749036667573E-4	0.0	0.0	13.512905373097945	0.0
8	4.687749036667573E-4	0.0	0.0	16.33399274336449	0.0
9	4.687749036667573E-4	0.0	0.0	17.413112571605367	0.0
10	4.687749036667573E-4	0.0	0.0	22.039452095892596	0.0
11	4.687749036667573E-4	0.0	0.0	24.47426894553773	0.0
12	4.687749036667573E-4	0.0	0.0	28.983883518811936	0.0
13	4.687749036667573E-4	0.0	0.0	29.964560617282793	0.0
14	4.687749036667573E-4	0.0	0.0	30.357862761459202	0.0
15	4.687749036667573E-4	0.0	0.0	31.327289262242058	0.0
16	4.687749036667573E-4	0.0	0.0	32.42562886153327	0.0
17	4.687749036667573E-4	0.0	0.0	33.73163574314886	0.0
18	4.687749036667573E-4	0.0	0.0	35.1524924761628	0.0
19	4.687749036667573E-4	0.0	0.0	36.35021235503136	0.0
20	9.375498073335146E-4	0.0	0.0	37.13822296809518	0.0
21	9.375498073335146E-4	0.0	0.0	37.90982645953066	0.0
22	9.375498073335146E-4	0.0	0.0	38.72127581777782	0.0
23	9.375498073335146E-4	0.0	0.0	39.435219996062294	0.0
24	9.375498073335146E-4	0.0	0.0	40.01931352603107	0.0
25	9.375498073335146E-4	0.0	0.0	40.49324495363816	0.0
26	9.375498073335146E-4	0.0	0.0	40.927799289337244	0.0
27	9.375498073335146E-4	0.0	0.0	41.41157498992134	0.0
28	9.375498073335146E-4	0.0	0.0	41.85831747311576	0.0
29	9.375498073335146E-4	0.0	0.0	42.31349790457618	0.0
30	9.375498073335146E-4	0.0	0.0	42.82540009938028	0.0
31	9.375498073335146E-4	0.0	0.0	43.266986058634366	0.0
32	9.375498073335146E-4	0.0	0.0	43.710447117503115	0.0
33	9.375498073335146E-4	0.0	0.0	44.10140538716119	0.0
34	9.375498073335146E-4	0.0	0.0	44.506426903929274	0.0
35	9.375498073335146E-4	0.0	0.0	44.95035673770169	0.0
36	9.375498073335146E-4	0.0	0.0	45.36522252744677	0.0
37	9.375498073335146E-4	0.0	0.0	45.764618745370846	0.0
38	9.375498073335146E-4	0.0	0.0	46.09229240303391	0.0
39	9.375498073335146E-4	0.0	0.0	46.49731391980199	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGGGT	35	1.2057171E-7	45.000004	4
AATGCGG	45	3.8198777E-10	45.000004	2
CGCTAGG	35	1.2057171E-7	45.000004	2
ATCCTTG	20	7.0203585E-4	45.0	29
TTCTAGG	20	7.0203585E-4	45.0	2
AGCGTCG	20	7.0203585E-4	45.0	17
CCTTAAC	25	3.8800932E-5	45.0	34
GTATTTA	20	7.0203585E-4	45.0	41
ACGCCCT	20	7.0203585E-4	45.0	39
GAATGCG	20	7.0203585E-4	45.0	1
TAAAGCG	20	7.0203585E-4	45.0	1
CTATATA	25	3.8800932E-5	45.0	40
ATCAGAC	25	3.8800932E-5	45.0	21
TGCGGGC	20	7.0203585E-4	45.0	4
TATGCGG	25	3.8800932E-5	45.0	2
CGTTCGG	25	3.8800932E-5	45.0	45
ACGGGAC	20	7.0203585E-4	45.0	5
CGCCTTA	25	3.8800932E-5	45.0	32
TATTGCT	20	7.0203585E-4	45.0	22
TAACCCG	25	3.8800932E-5	45.0	37
>>END_MODULE