FastQCFastQC Report
Sat 18 Jun 2016
SRR3551623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581320
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC75081.2915433840225694No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG64621.111608064405147No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC58000.997729305718021No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39880.6860249088281841No Hit
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC29880.5140026147388702TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT19190.33011078235739355TruSeq Adapter, Index 16 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT15150.2606137755453107No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT12540.21571595678799974No Hit
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC12340.21227551090621344TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTC11720.20161012867267597No Hit
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT10840.18647216679281634TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTA9760.16789375903117043No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCCCCTAT9720.16720566985481317No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCC6930.11921144980389457No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGA207.0304057E-445.00000437
ATAACGG253.8884187E-545.02
AGTACGG351.2105374E-745.02
CGTTTTT19200.041.367191
TACGAAT603.6379788E-1241.25000412
CGAATAT603.6379788E-1241.25000414
CGGTCTA603.6379788E-1241.25000431
TAGTAAG403.4542973E-739.3750041
CGATGAA5150.039.3203919
TTAATCG700.038.57142620
GACGTAG356.2429626E-638.5714261
CGTTGAT700.038.57142625
CACGAGG356.2429626E-638.5714262
GCCGATA356.2429626E-638.5714269
TACGGCT7050.038.297877
CTCACGA659.094947E-1238.07692724
CACGACG659.094947E-1238.07692726
CTACGAA659.094947E-1238.07692711
GCTACGA659.094947E-1238.07692710
GATGAAT5450.037.98164720
Produced by FastQC (version 0.11.5)