SRR3551622_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551622_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	362021
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	12133	3.3514630366746676	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	11884	3.2826824963192744	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	10815	2.987395758809572	No Hit
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	3415	0.9433154430267857	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	2958	0.8170796721737137	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2779	0.7676350266973463	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTTGCTCT	2039	0.5632269951190677	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	2025	0.5593598161432625	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC	1940	0.5358805152187304	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTA	1798	0.49665627132127693	No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	1163	0.3212520820615379	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	723	0.19971217139337222	Illumina PCR Primer Index 5 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	702	0.1939114029296643	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATG	625	0.1726419185627353	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC	517	0.14280939503509466	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTTTGCTC	476	0.13148408517737922	No Hit
TCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	465	0.12844558741067505	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGT	432	0.11933009411056264	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTAT	416	0.11491046099535661	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	373	0.10303269699824043	Illumina PCR Primer Index 5 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCCCA	35	1.2088458E-7	45.000004	33
TGATTCG	35	1.2088458E-7	45.000004	15
TCAAGAC	30	2.1613669E-6	45.000004	31
TTTAGCG	40	6.7921064E-9	45.0	1
AAGTATA	20	7.026877E-4	45.0	6
CGTGGCA	20	7.026877E-4	45.0	15
CGAACTG	20	7.026877E-4	45.0	34
ACACGAC	55	1.8189894E-12	45.0	26
GGTAAGT	20	7.026877E-4	45.0	8
CGACGAA	25	3.8854945E-5	45.0	19
TACGGGT	20	7.026877E-4	45.0	4
TATGAGG	40	6.7921064E-9	45.0	2
ACGGCTA	25	3.8854945E-5	45.0	30
GACGCAG	25	3.8854945E-5	45.0	1
GCGTGAG	20	7.026877E-4	45.0	1
CACGTCA	20	7.026877E-4	45.0	11
CGATCAT	25	3.8854945E-5	45.0	10
CCGTTTA	40	6.7921064E-9	45.0	41
TCGAATG	180	0.0	45.0	44
AACACGT	50	2.1827873E-11	45.0	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content