##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551622_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	362021
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.835009571267964	33.0	31.0	34.0	30.0	34.0
2	31.926667237535945	33.0	31.0	34.0	30.0	34.0
3	31.919485333723735	33.0	31.0	34.0	30.0	34.0
4	35.65005897447938	37.0	35.0	37.0	33.0	37.0
5	35.61563279478262	37.0	35.0	37.0	33.0	37.0
6	35.498026357586994	37.0	35.0	37.0	33.0	37.0
7	35.765974902008445	37.0	35.0	37.0	35.0	37.0
8	35.323760223854414	37.0	35.0	37.0	33.0	37.0
9	37.01361523226553	39.0	37.0	39.0	33.0	39.0
10	36.93441540684104	39.0	37.0	39.0	33.0	39.0
11	37.07615580311639	39.0	37.0	39.0	34.0	39.0
12	36.97457329823408	39.0	37.0	39.0	33.0	39.0
13	36.90744459575549	39.0	37.0	39.0	33.0	39.0
14	37.98768303496206	40.0	37.0	41.0	33.0	41.0
15	37.92282768126711	40.0	37.0	41.0	33.0	41.0
16	37.904801102698464	40.0	37.0	41.0	33.0	41.0
17	37.73584405324553	40.0	36.0	41.0	32.0	41.0
18	37.582772822571066	39.0	36.0	41.0	33.0	41.0
19	37.4502639349651	39.0	36.0	41.0	32.0	41.0
20	37.38900229544695	39.0	35.0	41.0	32.0	41.0
21	37.542916018684	39.0	36.0	41.0	33.0	41.0
22	37.63299919065469	39.0	36.0	41.0	33.0	41.0
23	37.54600699959395	40.0	36.0	41.0	33.0	41.0
24	37.469290455526064	39.0	35.0	41.0	32.0	41.0
25	37.312567502990156	39.0	35.0	41.0	32.0	41.0
26	37.39341916629146	39.0	35.0	41.0	32.0	41.0
27	37.28544200474558	39.0	35.0	41.0	32.0	41.0
28	37.15179782388314	39.0	35.0	41.0	31.0	41.0
29	37.22807240463951	39.0	35.0	41.0	32.0	41.0
30	36.938641681007454	39.0	35.0	41.0	31.0	41.0
31	36.955809193389335	39.0	35.0	41.0	31.0	41.0
32	36.74065040425832	39.0	35.0	41.0	31.0	41.0
33	36.630375033492534	39.0	35.0	41.0	30.0	41.0
34	36.5093544297154	39.0	35.0	41.0	30.0	41.0
35	36.32482646034346	39.0	35.0	41.0	30.0	41.0
36	36.20036959181926	39.0	35.0	41.0	29.0	41.0
37	36.069112012839035	39.0	35.0	41.0	29.0	41.0
38	36.018885644755414	39.0	35.0	41.0	28.0	41.0
39	35.906847945284945	39.0	35.0	41.0	28.0	41.0
40	35.87695465180197	39.0	35.0	41.0	28.0	41.0
41	35.84533770140406	39.0	35.0	40.0	27.0	41.0
42	35.73615342756359	39.0	35.0	41.0	27.0	41.0
43	35.72478392137473	39.0	35.0	41.0	28.0	41.0
44	35.92788263664263	39.0	35.0	41.0	28.0	41.0
45	35.8925034735554	39.0	35.0	41.0	29.0	41.0
46	35.789396195248344	39.0	35.0	40.0	28.0	41.0
47	35.53644125617022	38.0	35.0	40.0	27.0	41.0
48	35.55126912527174	38.0	35.0	40.0	27.0	41.0
49	35.64120865916618	38.0	35.0	40.0	28.0	41.0
50	35.41749511768654	38.0	35.0	40.0	27.0	41.0
51	34.630546294275746	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	8.0
11	8.0
12	4.0
13	9.0
14	11.0
15	15.0
16	21.0
17	49.0
18	130.0
19	315.0
20	837.0
21	1742.0
22	2658.0
23	3110.0
24	3280.0
25	3652.0
26	3998.0
27	4224.0
28	4339.0
29	4684.0
30	5590.0
31	7091.0
32	9249.0
33	12656.0
34	20878.0
35	27472.0
36	28527.0
37	41988.0
38	74361.0
39	101064.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.71197527215272	15.767317365567191	19.2961181809895	14.22458918129059
2	27.076329826170305	37.77239441910829	20.092757050005385	15.058518704716025
3	25.739667035890186	36.79206454874165	23.943638628698334	13.524629786669834
4	24.54885213841186	19.81514884495651	40.00872877540253	15.6272702412291
5	34.46043185340077	22.779893984050652	22.617748694136527	20.141925468412055
6	21.987398521080266	40.463950986268756	24.230362327047324	13.318288165603654
7	64.79265015012942	5.360738741675207	24.625919490858266	5.220691617337116
8	61.69200129274268	17.228006110142783	14.122661392571148	6.957331204543382
9	56.47379571903287	7.1004168266481775	14.089790371276804	22.335997083042145
10	40.23413006427804	20.4706356813555	22.015297455120006	17.279936799246453
11	32.572972286138096	19.5182047450286	26.556746708063894	21.352076260769405
12	24.49719767637789	16.775822397043267	32.190121567533375	26.536858359045468
13	25.06871148358797	19.630352935326954	38.41213631253436	16.88879926855072
14	19.377328939481412	30.70567729496355	27.785957168230574	22.131036597324464
15	15.100505219310481	20.885804967115167	41.058115413194265	22.955574400380087
16	16.816980230428623	23.36218064697904	28.883407316150166	30.937431806442167
17	17.340706754580534	25.905127050640708	32.01250756171603	24.741658633062723
18	17.95227348689717	23.664925515370655	31.415304637023816	26.967496360708353
19	19.41351468561216	24.41792050737388	27.071081511845996	29.097483295167958
20	23.766853304090095	24.335881067672872	33.06299910778657	18.83426652045047
21	19.887520337218007	31.871355529099137	28.477353523690613	19.763770609992235
22	18.4160587369241	22.05977001334177	31.247911032785392	28.27626021694874
23	21.985741158662066	28.07544313727657	28.87788277475616	21.060932929305206
24	22.29069584361128	24.846901146618567	25.799055855875764	27.063347153894384
25	17.910010745233013	34.41430193276081	24.787236099563284	22.888451222442896
26	19.50853679758909	22.12799809955776	31.845942638686708	26.517522464166444
27	26.478298220268993	24.279254518384295	26.433273207907824	22.809174053438888
28	17.519425668676682	25.951809425420073	33.05581720397435	23.47294770192889
29	26.71253877537491	19.304128766010813	30.541322188491826	23.442010270122452
30	21.350695125420902	27.019703276881728	31.826330516737976	19.80327108095939
31	23.790608832084324	20.25573102112861	28.045058159609525	27.90860198717754
32	27.169694575729032	23.66879269434646	30.248521494609427	18.91299123531508
33	26.97053485847506	19.426221130818377	25.409575687598235	28.19366832310833
34	25.99932048140854	19.337276014374858	27.281566538957687	27.38183696525892
35	23.17075528767668	18.415506282784698	33.16851784841211	25.24522058112651
36	24.152190066322117	22.79425779167507	28.42431792630814	24.62923421569467
37	18.494783451788706	20.08060305893857	38.86791097753998	22.556702511732745
38	22.024136721350416	21.35704834802401	28.89970471326249	27.71911021736308
39	23.537861063308483	20.905693316133593	29.72976705771212	25.8266785628458
40	23.73370605572605	19.149717834048303	32.69478842387596	24.421787686349685
41	19.02292960905583	22.307545694863006	25.084732653630592	33.58479204245058
42	20.049389400062427	20.017899514116586	36.56500589744794	23.36770518837305
43	24.933360219434782	19.690294209451938	26.468906499899177	28.907439071214103
44	23.22903919938346	19.653003555042385	31.058971717110335	26.05898552846382
45	20.870888705351348	17.932661364948444	30.764237433740032	30.432212495960183
46	28.237864654260385	23.253347181517096	26.81004693097914	21.698741233243375
47	18.896970065272455	21.542672938862662	37.43097776095862	22.129379234906263
48	22.3752213269396	21.832987589117756	27.87545473881349	27.91633634512915
49	20.2493777985255	17.677980006684695	37.40169769157038	24.670944503219427
50	22.422179928788662	18.472685286212677	31.473864775800298	27.63127000919836
51	22.438477325900983	18.228776783667247	27.61248656845874	31.72025932197303
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	93.0
1	282.5
2	472.0
3	2322.5
4	4173.0
5	2759.0
6	1345.0
7	1267.5
8	1190.0
9	1207.0
10	1224.0
11	1238.0
12	1252.0
13	1203.5
14	1155.0
15	1085.5
16	1016.0
17	1008.0
18	1000.0
19	963.0
20	926.0
21	914.0
22	902.0
23	971.0
24	1040.0
25	1293.5
26	1887.0
27	2227.0
28	2746.0
29	3265.0
30	3641.0
31	4017.0
32	4434.0
33	4851.0
34	5239.5
35	5628.0
36	6196.5
37	6765.0
38	7061.0
39	7357.0
40	8640.0
41	9923.0
42	11982.0
43	14041.0
44	16446.0
45	18851.0
46	44680.5
47	70510.0
48	53406.5
49	36303.0
50	36024.0
51	35745.0
52	29686.5
53	23628.0
54	21729.0
55	19830.0
56	18328.0
57	16826.0
58	15157.5
59	13489.0
60	13222.5
61	12956.0
62	11869.0
63	10782.0
64	9481.0
65	8180.0
66	7203.5
67	6227.0
68	5418.0
69	4609.0
70	3957.0
71	3305.0
72	2827.0
73	2349.0
74	1924.0
75	1127.5
76	756.0
77	583.0
78	410.0
79	332.5
80	255.0
81	154.0
82	53.0
83	44.5
84	36.0
85	23.0
86	10.0
87	6.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	362021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.79627321778301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.50331392718054	25.179343531132513
2	8.70065168162521	5.880992028099374
3	3.324174401493909	3.370341188893453
4	1.9413553613932095	2.624423048258111
5	1.421420832312763	2.4019363403145326
6	1.1970895029958635	2.4274298345632963
7	1.0872257457376597	2.5720924846650473
8	0.9177273257521391	2.481261075243571
9	0.8415849350357558	2.5598190960394707
>10	5.947569172216995	31.048112752940778
>50	0.06467418073762651	1.5106550255195277
>100	0.040933025783307916	2.5836074410808334
>500	0.0032746420626646334	0.7102292033898586
>1k	0.006549284125329267	5.0125525819299055
>5k	0.0	0.0
>10k+	0.002455981546998475	9.637204367929705
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	12133	3.3514630366746676	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	11884	3.2826824963192744	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	10815	2.987395758809572	No Hit
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	3415	0.9433154430267857	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	2958	0.8170796721737137	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2779	0.7676350266973463	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTTGCTCT	2039	0.5632269951190677	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	2025	0.5593598161432625	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC	1940	0.5358805152187304	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTA	1798	0.49665627132127693	No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	1163	0.3212520820615379	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	723	0.19971217139337222	Illumina PCR Primer Index 5 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	702	0.1939114029296643	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATG	625	0.1726419185627353	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC	517	0.14280939503509466	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTTTGCTC	476	0.13148408517737922	No Hit
TCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	465	0.12844558741067505	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGT	432	0.11933009411056264	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTAT	416	0.11491046099535661	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	373	0.10303269699824043	Illumina PCR Primer Index 5 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.762270697003765E-4	0.0	0.0	0.2044080315782786	0.0
2	2.762270697003765E-4	0.0	0.0	1.7247618232091508	0.0
3	2.762270697003765E-4	0.0	0.0	2.19379538756039	0.0
4	2.762270697003765E-4	0.0	0.0	3.2857209940859784	0.0
5	2.762270697003765E-4	0.0	0.0	8.03378809516575	0.0
6	2.762270697003765E-4	0.0	0.0	9.148364321406769	0.0
7	2.762270697003765E-4	0.0	0.0	10.884727681543335	0.0
8	2.762270697003765E-4	0.0	0.0	12.70036821068391	0.0
9	5.52454139400753E-4	0.0	0.0	13.312487397139945	0.0
10	5.52454139400753E-4	0.0	0.0	17.831009803298702	0.0
11	5.52454139400753E-4	0.0	0.0	19.39859842384834	0.0
12	5.52454139400753E-4	0.0	0.0	23.685090091458783	0.0
13	5.52454139400753E-4	0.0	0.0	24.4314556337892	0.0
14	5.52454139400753E-4	0.0	0.0	24.76182320915085	0.0
15	5.52454139400753E-4	0.0	0.0	25.625309029034227	0.0
16	5.52454139400753E-4	0.0	0.0	26.413937313028804	0.0
17	5.52454139400753E-4	0.0	0.0	27.243447203339034	0.0
18	5.52454139400753E-4	0.0	0.0	28.08455863057668	0.0
19	5.52454139400753E-4	0.0	0.0	29.241397598481857	0.0
20	5.52454139400753E-4	0.0	0.0	29.8623560511683	0.0
21	5.52454139400753E-4	0.0	0.0	30.41922982368426	0.0
22	5.52454139400753E-4	0.0	0.0	31.044331682416214	0.0
23	5.52454139400753E-4	0.0	0.0	31.551208355316405	0.0
24	5.52454139400753E-4	0.0	0.0	31.983503719397493	0.0
25	5.52454139400753E-4	0.0	0.0	32.344532499495884	0.0
26	5.52454139400753E-4	0.0	0.0	32.711362048057985	0.0
27	5.52454139400753E-4	0.0	0.0	33.17072766496971	0.0
28	5.52454139400753E-4	0.0	0.0	33.51794509158309	0.0
29	5.52454139400753E-4	0.0	0.0	33.863505155778256	0.0
30	5.52454139400753E-4	0.0	0.0	34.24552719317388	0.0
31	5.52454139400753E-4	0.0	0.0	34.6283779117786	0.0
32	5.52454139400753E-4	0.0	0.0	34.98084365271628	0.0
33	5.52454139400753E-4	0.0	0.0	35.31424972584463	0.0
34	5.52454139400753E-4	0.0	0.0	35.636882943254676	0.0
35	5.52454139400753E-4	0.0	0.0	35.98658641349535	0.0
36	5.52454139400753E-4	0.0	0.0	36.32524080094801	0.0
37	5.52454139400753E-4	0.0	0.0	36.66085669063397	0.0
38	5.52454139400753E-4	0.0	0.0	36.988461995298614	0.0
39	5.52454139400753E-4	0.0	0.0	37.30778048787225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCCCA	35	1.2088458E-7	45.000004	33
TGATTCG	35	1.2088458E-7	45.000004	15
TCAAGAC	30	2.1613669E-6	45.000004	31
TTTAGCG	40	6.7921064E-9	45.0	1
AAGTATA	20	7.026877E-4	45.0	6
CGTGGCA	20	7.026877E-4	45.0	15
CGAACTG	20	7.026877E-4	45.0	34
ACACGAC	55	1.8189894E-12	45.0	26
GGTAAGT	20	7.026877E-4	45.0	8
CGACGAA	25	3.8854945E-5	45.0	19
TACGGGT	20	7.026877E-4	45.0	4
TATGAGG	40	6.7921064E-9	45.0	2
ACGGCTA	25	3.8854945E-5	45.0	30
GACGCAG	25	3.8854945E-5	45.0	1
GCGTGAG	20	7.026877E-4	45.0	1
CACGTCA	20	7.026877E-4	45.0	11
CGATCAT	25	3.8854945E-5	45.0	10
CCGTTTA	40	6.7921064E-9	45.0	41
TCGAATG	180	0.0	45.0	44
AACACGT	50	2.1827873E-11	45.0	41
>>END_MODULE