FastQCFastQC Report
Sat 18 Jun 2016
SRR3551619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447083
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG84341.8864506143154627No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC79741.7835614416115129No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC79371.775285573372282No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC34440.7703267625921809No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31580.7063565378240729No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT18870.42206928020076806No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT18580.4155827888781278No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACGATT13200.29524719123742127No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTC12920.28898437202935473No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTA12090.270419586519729No Hit
GTGAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC10210.22836922897985384No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT7360.16462267632631972No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC6490.14516320235839877No Hit
GTGAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT6450.14426851390010356No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT6280.14046608795234888No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6080.1359926456608728No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC5360.11988825341155893No Hit
TCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC5110.11429645054721384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG502.1827873E-1145.0000042
CATCGAA253.886968E-545.00000442
CACAACG253.886968E-545.00000412
TCTACGG351.2097007E-745.02
GTTAACG207.0286565E-445.01
GTCGTTT207.0286565E-445.09
CGAATAT900.045.014
CGATCGA551.8189894E-1244.99999641
CGTTTTT15050.042.9069751
GCTACGA1000.042.75000410
CCGATCG650.041.53846440
CTTGCGG650.041.5384642
GATCGAA603.6379788E-1241.24999642
TACGAGG451.9237632E-840.02
GATGAAT7500.039.920
ATGAATG7450.039.5637621
CGCAAGG403.4518962E-739.3752
ATCGAAT1200.039.37499643
CGAATGC1150.039.13043645
AGCTACG1100.038.8636329