##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551619_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	447083
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.932940863329627	33.0	31.0	34.0	30.0	34.0
2	31.99912767875316	33.0	31.0	34.0	30.0	34.0
3	31.986581909846716	33.0	31.0	34.0	30.0	34.0
4	35.68687245992355	37.0	35.0	37.0	33.0	37.0
5	35.698132114171194	37.0	35.0	37.0	35.0	37.0
6	35.58668077292136	37.0	35.0	37.0	33.0	37.0
7	35.87395628999537	37.0	35.0	37.0	35.0	37.0
8	35.519449408722764	37.0	35.0	37.0	33.0	37.0
9	37.33209046195002	39.0	37.0	39.0	34.0	39.0
10	37.03338306310014	39.0	37.0	39.0	33.0	39.0
11	37.17118297944677	39.0	37.0	39.0	34.0	39.0
12	37.107653388744374	39.0	37.0	39.0	34.0	39.0
13	37.11472813772834	39.0	37.0	39.0	33.0	39.0
14	38.198084024666564	40.0	37.0	41.0	33.0	41.0
15	38.15515239899526	40.0	37.0	41.0	33.0	41.0
16	38.048407119036064	40.0	37.0	41.0	33.0	41.0
17	37.96413417642809	40.0	37.0	41.0	33.0	41.0
18	37.81255382110257	39.0	37.0	41.0	33.0	41.0
19	37.610519746892635	39.0	36.0	41.0	33.0	41.0
20	37.72610902226209	39.0	36.0	41.0	33.0	41.0
21	37.72508013053505	39.0	36.0	41.0	33.0	41.0
22	37.874334295869	40.0	36.0	41.0	33.0	41.0
23	37.8524502161791	40.0	36.0	41.0	33.0	41.0
24	37.746935132850055	40.0	36.0	41.0	33.0	41.0
25	37.49015283515589	39.0	35.0	41.0	33.0	41.0
26	37.65300850177707	39.0	36.0	41.0	33.0	41.0
27	37.69126985369607	40.0	36.0	41.0	33.0	41.0
28	37.5639087149366	40.0	36.0	41.0	33.0	41.0
29	37.620258430761176	40.0	36.0	41.0	33.0	41.0
30	37.362474081993724	40.0	35.0	41.0	33.0	41.0
31	37.37707763435425	39.0	36.0	41.0	33.0	41.0
32	37.18707935662953	40.0	35.0	41.0	32.0	41.0
33	37.08042801895845	40.0	35.0	41.0	32.0	41.0
34	36.894388737661686	40.0	35.0	41.0	31.0	41.0
35	36.796827434726886	40.0	35.0	41.0	31.0	41.0
36	36.6530979706229	40.0	35.0	41.0	31.0	41.0
37	36.60538647186316	39.0	35.0	41.0	31.0	41.0
38	36.581666044112616	39.0	35.0	41.0	30.0	41.0
39	36.48580241252743	39.0	35.0	41.0	30.0	41.0
40	36.40215575184026	39.0	35.0	41.0	30.0	41.0
41	36.23971611535218	39.0	35.0	41.0	30.0	41.0
42	36.20557256706249	39.0	35.0	41.0	30.0	41.0
43	36.2257746324508	39.0	35.0	41.0	30.0	41.0
44	36.372590771735894	39.0	35.0	41.0	30.0	41.0
45	36.34129456946473	39.0	35.0	41.0	30.0	41.0
46	36.25761659468152	39.0	35.0	41.0	30.0	41.0
47	36.14041911680829	39.0	35.0	41.0	30.0	41.0
48	36.13187484203157	39.0	35.0	41.0	30.0	41.0
49	36.13676431445615	39.0	35.0	41.0	30.0	41.0
50	35.9877450048425	39.0	35.0	41.0	30.0	41.0
51	35.17305064160346	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	10.0
10	17.0
11	9.0
12	8.0
13	16.0
14	19.0
15	18.0
16	32.0
17	56.0
18	137.0
19	240.0
20	567.0
21	1189.0
22	1820.0
23	2535.0
24	3063.0
25	3827.0
26	4544.0
27	4979.0
28	5043.0
29	5315.0
30	6144.0
31	8051.0
32	10367.0
33	14758.0
34	25667.0
35	33574.0
36	34352.0
37	50868.0
38	94064.0
39	135748.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.40887038871977	17.267710917212238	21.723706783751563	13.599711910316428
2	29.561848694761377	31.668392669817464	23.550660615590395	15.21909801983077
3	28.71077629880805	30.703023823316922	25.96766148567492	14.618538392200106
4	25.101603058045153	23.52851707624759	34.46653082313575	16.903349042571513
5	29.82309772458358	26.58611488247149	24.47778153049881	19.113005862446123
6	22.910287351565593	37.47357873146597	25.744660387444835	13.8714735295236
7	70.57548598358694	5.9762952292974685	18.56411449328201	4.884104293833583
8	69.04937114584988	12.665433487741652	12.184985785637119	6.100209580771356
9	63.38465117215372	7.425243187506571	14.237401108966344	14.95270453137337
10	39.03346805850368	22.46898226951148	21.798636942133786	16.698912729851056
11	31.507572419438894	22.349988704558214	25.935005356947144	20.207433519055744
12	25.429282705895773	19.637740643236267	31.12486943140312	23.80810721946484
13	24.71509764406162	22.123632524609523	35.50593513956022	17.655334691768644
14	21.178394168420628	28.30033796856512	28.739630001588072	21.78163786142618
15	18.36504631131132	23.615301856702224	37.297548777296385	20.72210305469007
16	20.443183927816534	23.88326104996164	29.762258909419504	25.911296112802322
17	20.867266257048467	25.274277930496126	31.251244176137316	22.607211636318087
18	20.888515107932978	23.986597566894737	30.686024742609312	24.43886258256297
19	20.887620419474683	26.16941373302049	28.229433908245227	24.7135319392596
20	23.97094051887457	25.359944350377894	31.058886157603844	19.61022897314369
21	21.697760818461003	30.50842908363771	28.092322902011485	19.701487195889804
22	20.028272155282128	23.59450035004686	30.371989093747693	26.005238400923318
23	21.655039444577405	28.00688015424429	28.7812330148988	21.556847386279504
24	22.51438770876996	24.875023205981886	28.129228800916163	24.481360284331995
25	19.632372512486494	31.393499641006255	26.56218196621209	22.411945880295157
26	19.628346414424165	24.388312684669287	31.366658987257402	24.616681913649145
27	24.83945933976465	25.93768942232203	26.214819172278975	23.008032065634346
28	18.954869677442442	26.17992632240546	31.309846270155656	23.555357729996445
29	22.66357700919069	23.479980227385074	30.133778291726593	23.722664471697648
30	23.13597251517056	27.00125032712047	28.011800940764914	21.85097621694406
31	24.729412659394338	23.677035360324595	24.186113093094573	27.407438887186498
32	25.03986955442278	29.113609777155474	24.705703415249516	21.14081725317223
33	23.35919728551522	23.72154611112478	24.453401270010268	28.465855333349737
34	24.08009251078659	24.645535616429164	27.267867487692442	24.006504385091805
35	24.0543702176106	26.027158268151553	25.371798972450303	24.546672541787544
36	22.421116436992683	25.526803747849954	28.47927566022416	23.5728041549332
37	23.644155559482243	27.57206156351282	28.070626706897823	20.713156170107116
38	20.11460959150762	26.6731233350407	24.375563374138583	28.836703699313105
39	24.607287684837043	26.896348105385353	26.543393508587894	21.952970701189713
40	21.952076012731418	25.2693571439755	27.97221097648535	24.80635586680773
41	22.261861891416135	25.272935897808686	27.84046810100138	24.6247341097738
42	21.084451880299632	26.09224685349253	30.653815958110687	22.169485308097155
43	22.589765211381334	23.37172292393135	29.426974409673374	24.611537455013945
44	23.122104844066985	22.820594833621495	28.785706457190273	25.27159386512124
45	21.668683443566408	22.574555507590315	27.42175390251922	28.33500714632406
46	25.76411986141276	26.103206787106643	25.82831375829544	22.304359593185158
47	19.58070425401995	24.13668155577376	33.40923273754538	22.873381452660915
48	22.468758597396903	24.55002762350615	27.330272007658536	25.65094177143841
49	21.263836916187824	21.882066640869816	32.797265832071446	24.056830610870914
50	22.191181503210814	21.501600373979777	29.99487790857626	26.31234021423315
51	20.77086357566716	21.645197871536155	28.07152139535612	29.512417157440563
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109.0
1	288.0
2	467.0
3	2694.0
4	4921.0
5	3269.5
6	1618.0
7	1555.5
8	1493.0
9	1525.5
10	1558.0
11	1591.5
12	1625.0
13	1673.0
14	1721.0
15	1638.5
16	1556.0
17	1493.0
18	1430.0
19	1382.0
20	1334.0
21	1374.0
22	1414.0
23	1469.5
24	1525.0
25	1808.5
26	2588.0
27	3084.0
28	3531.5
29	3979.0
30	4521.5
31	5064.0
32	6145.5
33	7227.0
34	8093.5
35	8960.0
36	9740.0
37	10520.0
38	11260.0
39	12000.0
40	13949.0
41	15898.0
42	18046.0
43	20194.0
44	23701.0
45	27208.0
46	47282.0
47	67356.0
48	56946.5
49	46537.0
50	44794.5
51	43052.0
52	37452.0
53	31852.0
54	28653.5
55	25455.0
56	23028.0
57	20601.0
58	19312.5
59	18024.0
60	16245.0
61	14466.0
62	13389.0
63	12312.0
64	11088.0
65	9864.0
66	8309.0
67	6754.0
68	5682.5
69	4611.0
70	4039.0
71	3467.0
72	3008.5
73	2550.0
74	1967.0
75	1164.0
76	944.0
77	673.5
78	403.0
79	312.5
80	222.0
81	186.5
82	151.0
83	89.0
84	27.0
85	30.5
86	34.0
87	23.5
88	13.0
89	8.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	447083.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.64659903001377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.50890800290286	27.304980757465646
2	8.90950720332536	6.530062760705675
3	3.4304559900935496	3.7714363547720153
4	2.0769809969296467	3.0445715914975606
5	1.5350599497740738	2.8127363233201774
6	1.1631494335301256	2.55752825415397
7	1.02101203256266	2.6191632993500207
8	0.8844232167575307	2.5928882397878543
9	0.762844635144818	2.516009531971436
>10	5.607238710286168	32.94434239160616
>50	0.05510844257555203	1.3925829567827928
>100	0.034289697602565714	2.0747376761865457
>500	0.004286212200320714	0.9678070081540744
>1k	0.004898528228937959	3.4083052740209214
>5k	0.0018369480858517345	5.4628475802251195
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	8434	1.8864506143154627	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	7974	1.7835614416115129	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	7937	1.775285573372282	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	3444	0.7703267625921809	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3158	0.7063565378240729	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT	1887	0.42206928020076806	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	1858	0.4155827888781278	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACGATT	1320	0.29524719123742127	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTC	1292	0.28898437202935473	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTA	1209	0.270419586519729	No Hit
GTGAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	1021	0.22836922897985384	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	736	0.16462267632631972	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC	649	0.14516320235839877	No Hit
GTGAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT	645	0.14426851390010356	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT	628	0.14046608795234888	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	608	0.1359926456608728	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	536	0.11988825341155893	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	511	0.11429645054721384	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.473442291476079E-4	0.0	0.0	0.17043815130523862	0.0
2	4.473442291476079E-4	0.0	0.0	1.282312232851618	0.0
3	4.473442291476079E-4	0.0	0.0	1.6538316151587065	0.0
4	4.473442291476079E-4	0.0	0.0	2.5655191541615316	0.0
5	4.473442291476079E-4	0.0	0.0	5.577935193241523	0.0
6	4.473442291476079E-4	0.0	0.0	6.751542778410273	0.0
7	4.473442291476079E-4	0.0	0.0	8.214358407722951	0.0
8	4.473442291476079E-4	0.0	0.0	9.935962673597519	0.0
9	4.473442291476079E-4	0.0	0.0	10.623083409568245	0.0
10	4.473442291476079E-4	0.0	0.0	13.796319699026803	0.0
11	4.473442291476079E-4	0.0	0.0	15.205230348727193	0.0
12	4.473442291476079E-4	0.0	0.0	18.430358568766874	0.0
13	4.473442291476079E-4	0.0	0.0	19.0047485589924	0.0
14	4.473442291476079E-4	0.0	0.0	19.340033058738534	0.0
15	4.473442291476079E-4	0.0	0.0	19.95244730844161	0.0
16	6.710163437214119E-4	0.0	0.0	20.666632370275764	0.0
17	6.710163437214119E-4	0.0	0.0	21.49533755477171	0.0
18	6.710163437214119E-4	0.0	0.0	22.374145292932184	0.0
19	6.710163437214119E-4	0.0	0.0	23.21940221390659	0.0
20	6.710163437214119E-4	0.0	0.0	23.784621647434594	0.0
21	6.710163437214119E-4	0.0	0.0	24.38719432409642	0.0
22	6.710163437214119E-4	0.0	0.0	25.02443617851719	0.0
23	6.710163437214119E-4	0.0	0.0	25.584734825524567	0.0
24	6.710163437214119E-4	0.0	0.0	26.064511511285378	0.0
25	6.710163437214119E-4	0.0	0.0	26.46510826848706	0.0
26	6.710163437214119E-4	0.0	0.0	26.865481353574168	0.0
27	6.710163437214119E-4	0.0	0.0	27.292247748180987	0.0
28	6.710163437214119E-4	0.0	0.0	27.68859473520577	0.0
29	6.710163437214119E-4	0.0	0.0	28.112229720208553	0.0
30	6.710163437214119E-4	0.0	0.0	28.591111717511065	0.0
31	6.710163437214119E-4	0.0	0.0	28.991484802598176	0.0
32	6.710163437214119E-4	0.0	0.0	29.39208155979986	0.0
33	8.946884582952158E-4	0.0	0.0	29.76717969594013	0.0
34	8.946884582952158E-4	0.0	0.0	30.152343077236218	0.0
35	8.946884582952158E-4	0.0	0.0	30.551597801750457	0.0
36	8.946884582952158E-4	0.0	0.0	30.9363138388174	0.0
37	8.946884582952158E-4	0.0	0.0	31.332884497956755	0.0
38	8.946884582952158E-4	0.0	0.0	31.748019942605737	0.0
39	8.946884582952158E-4	0.0	0.0	32.13720942196415	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	50	2.1827873E-11	45.000004	2
CATCGAA	25	3.886968E-5	45.000004	42
CACAACG	25	3.886968E-5	45.000004	12
TCTACGG	35	1.2097007E-7	45.0	2
GTTAACG	20	7.0286565E-4	45.0	1
GTCGTTT	20	7.0286565E-4	45.0	9
CGAATAT	90	0.0	45.0	14
CGATCGA	55	1.8189894E-12	44.999996	41
CGTTTTT	1505	0.0	42.906975	1
GCTACGA	100	0.0	42.750004	10
CCGATCG	65	0.0	41.538464	40
CTTGCGG	65	0.0	41.538464	2
GATCGAA	60	3.6379788E-12	41.249996	42
TACGAGG	45	1.9237632E-8	40.0	2
GATGAAT	750	0.0	39.9	20
ATGAATG	745	0.0	39.56376	21
CGCAAGG	40	3.4518962E-7	39.375	2
ATCGAAT	120	0.0	39.374996	43
CGAATGC	115	0.0	39.130436	45
AGCTACG	110	0.0	38.863632	9
>>END_MODULE