FastQCFastQC Report
Sat 18 Jun 2016
SRR3551618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510740
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG88521.7331714766808943No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC87801.719074284371696No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC75771.483533696205506No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC36010.7050554097975487TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26220.5133727532599757No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT21150.41410502408270355No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT20520.4017699808121549No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTCCTTT14540.28468496691075695No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTA14130.27665739906801895No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTC14120.27646160473039116No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT13440.2631475897717038TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC12430.24337236167130044TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC7610.14899949093472217No Hit
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC6860.13431491561264047TruSeq Adapter, Index 19 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT5620.11003641774679877No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.0296E-445.00000444
TGGACCG207.0296E-445.00000415
TTATCCG207.0296E-445.0000041
CGGATAT207.0296E-445.00000421
TATTACG207.0296E-445.0000041
GCGATAT207.0296E-445.0000049
CAAGATA551.8189894E-1245.00000438
TTAAACG207.0296E-445.0000041
ATACGCT207.0296E-445.0000049
CAGTACG207.0296E-445.0000041
TACGAAT253.8877508E-545.012
CGGTCTA253.8877508E-545.031
AATACGG253.8877508E-545.02
GATGAAT6400.042.53906620
CGTTTTT14300.042.3251761
TAAACGG501.0786607E-940.52
CCGATGA6450.040.4651218
TGAATGA6750.040.33333222
ATGAATG6850.040.07299421
TGGGCGA2250.040.06