##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551618_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	510740
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93462427066609	33.0	31.0	34.0	30.0	34.0
2	32.01792888749657	33.0	31.0	34.0	30.0	34.0
3	32.00577201707327	33.0	31.0	34.0	30.0	34.0
4	35.698067509887615	37.0	35.0	37.0	33.0	37.0
5	35.70259427497357	37.0	35.0	37.0	35.0	37.0
6	35.582108313427575	37.0	35.0	37.0	33.0	37.0
7	35.87062889141246	37.0	35.0	37.0	35.0	37.0
8	35.55910639464307	37.0	35.0	37.0	33.0	37.0
9	37.31049066061009	39.0	37.0	39.0	34.0	39.0
10	37.07922230489094	39.0	37.0	39.0	34.0	39.0
11	37.19737243998904	39.0	37.0	39.0	34.0	39.0
12	37.127975094960256	39.0	37.0	39.0	34.0	39.0
13	37.13357872890316	39.0	37.0	39.0	33.0	39.0
14	38.22034694756628	40.0	38.0	41.0	33.0	41.0
15	38.20637506363316	40.0	37.0	41.0	33.0	41.0
16	38.070437012961584	40.0	37.0	41.0	33.0	41.0
17	38.01468653326546	40.0	37.0	41.0	33.0	41.0
18	37.82794768375299	39.0	37.0	41.0	33.0	41.0
19	37.67923992638133	39.0	36.0	41.0	33.0	41.0
20	37.84590985628696	40.0	36.0	41.0	33.0	41.0
21	37.83069272036653	39.0	36.0	41.0	33.0	41.0
22	37.96840858362376	40.0	37.0	41.0	34.0	41.0
23	37.96826565375729	40.0	37.0	41.0	34.0	41.0
24	37.83084739789325	40.0	36.0	41.0	33.0	41.0
25	37.57180169949485	39.0	36.0	41.0	33.0	41.0
26	37.72378509613502	40.0	36.0	41.0	33.0	41.0
27	37.75997963738889	40.0	36.0	41.0	33.0	41.0
28	37.642142773231	40.0	36.0	41.0	33.0	41.0
29	37.72215804518933	40.0	36.0	41.0	33.0	41.0
30	37.46213141715942	40.0	36.0	41.0	33.0	41.0
31	37.46719074284372	40.0	36.0	41.0	33.0	41.0
32	37.37956690292517	40.0	36.0	41.0	33.0	41.0
33	37.31838704624662	40.0	36.0	41.0	33.0	41.0
34	37.216507420605396	40.0	36.0	41.0	32.0	41.0
35	37.18935661980655	40.0	36.0	41.0	32.0	41.0
36	37.00952539452559	40.0	35.0	41.0	32.0	41.0
37	36.991373301484124	40.0	35.0	41.0	32.0	41.0
38	36.91565767318009	39.0	35.0	41.0	31.0	41.0
39	36.76897051337275	39.0	35.0	41.0	31.0	41.0
40	36.725729333907665	39.0	35.0	41.0	31.0	41.0
41	36.55373771390531	39.0	35.0	41.0	30.0	41.0
42	36.568712064847084	39.0	35.0	41.0	31.0	41.0
43	36.549729803814074	39.0	35.0	41.0	31.0	41.0
44	36.63566198065552	39.0	35.0	41.0	31.0	41.0
45	36.60573677409249	39.0	35.0	41.0	31.0	41.0
46	36.524848259388335	39.0	35.0	41.0	31.0	41.0
47	36.39709245408623	39.0	35.0	41.0	31.0	41.0
48	36.367139836315935	39.0	35.0	41.0	31.0	41.0
49	36.394631319262245	39.0	35.0	41.0	31.0	41.0
50	36.25778086697733	39.0	35.0	41.0	31.0	41.0
51	35.43042644006735	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	13.0
11	13.0
12	17.0
13	14.0
14	21.0
15	27.0
16	44.0
17	77.0
18	119.0
19	294.0
20	571.0
21	1111.0
22	1829.0
23	2482.0
24	2887.0
25	3543.0
26	4129.0
27	4826.0
28	5084.0
29	5647.0
30	6614.0
31	8680.0
32	11634.0
33	16664.0
34	29638.0
35	38567.0
36	40406.0
37	59766.0
38	108154.0
39	157786.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.57203273681325	17.63304225241806	22.056036339429063	13.738888671339625
2	29.447076790539217	30.709950268238245	23.2576261894506	16.58534675177194
3	28.957199357794572	30.160355562517132	25.376316716920545	15.506128362767749
4	26.624701413635115	22.683948780201277	32.774209969847675	17.917139836315936
5	29.838861260132354	26.669929905627125	23.85029564944982	19.640913184790694
6	24.331362337001213	37.292164310608136	23.467321925049927	14.909151427340722
7	71.38563652739163	5.940204409288484	17.16274425343619	5.511414809883698
8	70.22868778634923	11.320632807299214	11.627050945686651	6.823628460664917
9	64.0188745741473	7.404354466068841	13.797235383952696	14.779535575831146
10	39.70885381994753	22.452715667462897	20.72326428319693	17.115166229392646
11	31.25954497395935	21.808160707992325	25.86971844774249	21.06257587030583
12	25.76438109409876	18.812898930962916	31.446724360731487	23.975995614206838
13	24.59450992677292	20.973881035360456	35.268434036887655	19.16317500097897
14	20.53216900967224	27.69491326310843	29.043544660688415	22.72937306653092
15	17.871715549986295	22.711359987469162	37.668481027528685	21.74844343501586
16	19.69377765595019	22.916160864627795	30.26138544073305	27.12867603868896
17	19.758781376042606	24.74018091396797	30.878137604260488	24.62290010572894
18	20.253553667227944	23.758859693777655	30.664525981908604	25.3230606570858
19	21.230371617652818	25.548811528370603	27.15569565728159	26.06512119669499
20	24.27438618475154	24.62838234718252	29.70474213885734	21.3924893292086
21	22.514782472490896	28.523319105611467	27.21267180953127	21.74922661236637
22	20.467361083917453	22.913419743901006	28.828954066648393	27.790265105533145
23	21.912127501272664	26.672279437678664	27.833144065473626	23.58244899557505
24	22.700591298899635	23.80663351215883	27.404941849081727	26.087833339859813
25	20.21733171476681	29.15710537651251	25.77475819399303	24.85080471472765
26	20.106120530994243	23.830520421349416	30.03602615812351	26.027332889532833
27	25.548811528370603	24.45197164897991	25.808434820065003	24.190782002584484
28	19.992559815170143	24.55574264792262	30.467361083917453	24.98433645298978
29	23.31988878881623	21.52053882601715	30.24572189372283	24.913850491443785
30	24.33155813133884	25.101029878215925	26.681481771547165	23.885930218898068
31	23.57070133531738	21.798175196773308	24.53929592356189	30.091827544347417
32	24.855503778830716	23.96013627285899	28.06750988761405	23.116850060696244
33	23.048322042526532	21.630771038101578	25.23906488624349	30.081842033128403
34	21.369581391706152	21.36723185965462	28.65802560989936	28.605161138739867
35	23.62963543094334	20.438383521948545	29.968868700317188	25.96311234679093
36	21.72651446920155	22.88874965735991	29.504444531464152	25.880291341974388
37	22.076007361867095	22.115166229392646	31.746681285977207	24.06214512276305
38	20.230841524063123	22.623252535536672	27.405920820769865	29.73998511963034
39	22.229314328229627	21.602772447820808	30.02858597329365	26.139327250655914
40	21.262677683361396	20.424873712652232	32.204840036026155	26.107608567960217
41	19.67733093158946	22.43313623370012	29.93871637232251	27.950816462387905
42	20.98582448995575	21.794063515683128	33.607902259466655	23.612209734894467
43	23.54916395817833	20.58601245251987	30.354191956768613	25.510631632533187
44	22.896189842189763	21.097818851078827	29.10659043740455	26.89940086932686
45	21.497630888514703	19.735286055527272	28.981086266985155	29.785996788972863
46	25.171124251086656	22.76833613971884	28.50785135293887	23.55268825625563
47	19.866664056075496	21.45925519833966	34.177272193288175	24.496808552296667
48	21.89940086932686	21.600227121431647	28.814073696988686	27.68629831225281
49	21.384265967028234	19.535771625484593	33.75866389944003	25.321298508047146
50	21.252692172142382	19.63523514899949	31.253866938168144	27.85820574068998
51	21.28108235109841	19.582370677839997	28.46869248541332	30.667854485648277
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	141.0
1	385.0
2	629.0
3	2415.5
4	4202.0
5	2840.5
6	1479.0
7	1460.5
8	1442.0
9	1462.0
10	1482.0
11	1399.0
12	1316.0
13	1285.5
14	1255.0
15	1288.5
16	1322.0
17	1229.5
18	1137.0
19	1180.0
20	1223.0
21	1265.5
22	1308.0
23	1514.0
24	1720.0
25	2033.0
26	2835.0
27	3324.0
28	4121.5
29	4919.0
30	5271.5
31	5624.0
32	6416.5
33	7209.0
34	7844.0
35	8479.0
36	9476.5
37	10474.0
38	11462.0
39	12450.0
40	13995.5
41	15541.0
42	17972.0
43	20403.0
44	23792.5
45	27182.0
46	49447.5
47	71713.0
48	61883.5
49	52054.0
50	51003.0
51	49952.0
52	42975.0
53	35998.0
54	32449.5
55	28901.0
56	26840.0
57	24779.0
58	23727.5
59	22676.0
60	22170.0
61	21664.0
62	20120.5
63	18577.0
64	16942.5
65	15308.0
66	12861.5
67	10415.0
68	8857.0
69	7299.0
70	6363.0
71	5427.0
72	4679.5
73	3932.0
74	3239.0
75	2003.5
76	1461.0
77	1137.0
78	813.0
79	595.5
80	378.0
81	243.5
82	109.0
83	90.5
84	72.0
85	56.0
86	40.0
87	25.5
88	11.0
89	8.0
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	510740.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.79741032064676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.38145037033718	24.067215377201247
2	9.026117610215033	5.92066565729276
3	3.3931662559096436	3.3386119794372378
4	1.9071918960317749	2.502038206974656
5	1.3424940944810082	2.2015164834869374
6	1.0732489365728997	2.1119871449387504
7	0.9681706462047835	2.2227442960788815
8	0.8490979033208725	2.2278569870092406
9	0.7349319357795744	2.169347782995892
>10	7.200606261663835	40.30384416116021
>50	0.07075651727689818	1.5548224550935419
>100	0.04377309967130141	2.632932333517877
>500	0.001798894507039784	0.39509718091107077
>1k	0.005396683521119352	3.3936271547045482
>5k	0.001798894507039784	4.957692799197204
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	8852	1.7331714766808943	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	8780	1.719074284371696	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	7577	1.483533696205506	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	3601	0.7050554097975487	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2622	0.5133727532599757	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT	2115	0.41410502408270355	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	2052	0.4017699808121549	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTCCTTT	1454	0.28468496691075695	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTA	1413	0.27665739906801895	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTC	1412	0.27646160473039116	No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	1344	0.2631475897717038	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1243	0.24337236167130044	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC	761	0.14899949093472217	No Hit
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	686	0.13431491561264047	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT	562	0.11003641774679877	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.957943376277558E-4	0.0	0.0	0.2711751576144418	0.0
2	1.957943376277558E-4	0.0	0.0	1.483337901867878	0.0
3	1.957943376277558E-4	0.0	0.0	1.8759055488115284	0.0
4	1.957943376277558E-4	0.0	0.0	2.8123898656850845	0.0
5	1.957943376277558E-4	0.0	0.0	5.929631515056585	0.0
6	1.957943376277558E-4	0.0	0.0	7.110858753964835	0.0
7	1.957943376277558E-4	0.0	0.0	8.553079844930885	0.0
8	1.957943376277558E-4	0.0	0.0	10.45365548028351	0.0
9	1.957943376277558E-4	0.0	0.0	11.264831421075302	0.0
10	1.957943376277558E-4	0.0	0.0	14.382464659122059	0.0
11	1.957943376277558E-4	0.0	0.0	15.9911109370717	0.0
12	1.957943376277558E-4	0.0	0.0	18.813290519638173	0.0
13	3.915886752555116E-4	0.0	0.0	19.450992677291772	0.0
14	3.915886752555116E-4	0.0	0.0	19.72353839526961	0.0
15	3.915886752555116E-4	0.0	0.0	20.378470454634453	0.0
16	3.915886752555116E-4	0.0	0.0	21.11074127736226	0.0
17	3.915886752555116E-4	0.0	0.0	21.95324431217449	0.0
18	3.915886752555116E-4	0.0	0.0	22.83471042017465	0.0
19	3.915886752555116E-4	0.0	0.0	23.68954849825743	0.0
20	3.915886752555116E-4	0.0	0.0	24.25833104906606	0.0
21	3.915886752555116E-4	0.0	0.0	24.867643027763638	0.0
22	3.915886752555116E-4	0.0	0.0	25.513568547597604	0.0
23	3.915886752555116E-4	0.0	0.0	26.070211849473313	0.0
24	3.915886752555116E-4	0.0	0.0	26.53894349375416	0.0
25	5.873830128832674E-4	0.0	0.0	26.945216744331756	0.0
26	5.873830128832674E-4	0.0	0.0	27.331518972471315	0.0
27	5.873830128832674E-4	0.0	0.0	27.767944551043584	0.0
28	5.873830128832674E-4	0.0	0.0	28.139757998198693	0.0
29	7.831773505110232E-4	0.0	0.0	28.539178446959315	0.0
30	7.831773505110232E-4	0.0	0.0	28.976974585894975	0.0
31	7.831773505110232E-4	0.0	0.0	29.378352978031874	0.0
32	7.831773505110232E-4	0.0	0.0	29.780514547519285	0.0
33	7.831773505110232E-4	0.0	0.0	30.15526490973881	0.0
34	7.831773505110232E-4	0.0	0.0	30.53236480400987	0.0
35	7.831773505110232E-4	0.0	0.0	30.90652778321651	0.0
36	9.78971688138779E-4	0.0	0.0	31.267964130477345	0.0
37	9.78971688138779E-4	0.0	0.0	31.6591612170576	0.0
38	9.78971688138779E-4	0.0	0.0	32.03156204722559	0.0
39	9.78971688138779E-4	0.0	0.0	32.40885773583428	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.0296E-4	45.000004	44
TGGACCG	20	7.0296E-4	45.000004	15
TTATCCG	20	7.0296E-4	45.000004	1
CGGATAT	20	7.0296E-4	45.000004	21
TATTACG	20	7.0296E-4	45.000004	1
GCGATAT	20	7.0296E-4	45.000004	9
CAAGATA	55	1.8189894E-12	45.000004	38
TTAAACG	20	7.0296E-4	45.000004	1
ATACGCT	20	7.0296E-4	45.000004	9
CAGTACG	20	7.0296E-4	45.000004	1
TACGAAT	25	3.8877508E-5	45.0	12
CGGTCTA	25	3.8877508E-5	45.0	31
AATACGG	25	3.8877508E-5	45.0	2
GATGAAT	640	0.0	42.539066	20
CGTTTTT	1430	0.0	42.325176	1
TAAACGG	50	1.0786607E-9	40.5	2
CCGATGA	645	0.0	40.46512	18
TGAATGA	675	0.0	40.333332	22
ATGAATG	685	0.0	40.072994	21
TGGGCGA	225	0.0	40.0	6
>>END_MODULE