FastQCFastQC Report
Sat 18 Jun 2016
SRR3551616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323602
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC67102.073534774197934No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61421.8980105190944432No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC49961.5438717931285963No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG49761.5376913616108676No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC17920.5537666639884797No Hit
GAACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCT15690.4848548525658062No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCT11680.3609372006353484No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTC9250.2858449576949462No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGAATCAT8870.27410213781126197No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCGAATCATCGTA7710.2382556350084363No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT7430.2296030308836163No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCC7420.22929400930772986No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4690.14493111909073492No Hit
CGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG4340.13411536393470994No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC4270.13195221290350492No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCAACCATCGAATGC3760.11619211253329707No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT3520.10877559471202279No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATTA207.0257665E-445.0000048
TCGAATA207.0257665E-445.00000444
GCGTAAG207.0257665E-445.0000041
AAACGAT351.2083183E-745.016
CTACGAA502.1827873E-1145.011
CTCGAGG302.160652E-644.9999962
CCGCGAA650.044.99999641
CATCGAA1750.043.71428742
ATCGAAT1750.043.71428743
CGTTTTT27450.042.950821
TCGAATG1600.042.18750444
CCGATGA7150.042.1678318
CTCATGC750.042.023
GATGAAT8000.041.9062520
ACCCGCG700.041.78571339
CCCGCGA700.041.78571340
CACCCGC650.041.5384638
CGATGAA7750.041.51612519
TACGAAT556.002665E-1140.90909212
ATGAATG8050.040.80745321