##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551616_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323602
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83729395986428	33.0	31.0	34.0	30.0	34.0
2	31.961647332216735	33.0	31.0	34.0	30.0	34.0
3	31.900269466814173	33.0	31.0	34.0	30.0	34.0
4	35.596936978139816	37.0	35.0	37.0	33.0	37.0
5	35.61895476542172	37.0	35.0	37.0	33.0	37.0
6	35.493485825180315	37.0	35.0	37.0	33.0	37.0
7	35.78442036823011	37.0	35.0	37.0	35.0	37.0
8	35.46400207662499	37.0	35.0	37.0	33.0	37.0
9	37.23225443600472	39.0	37.0	39.0	34.0	39.0
10	37.00345486121841	39.0	37.0	39.0	33.0	39.0
11	37.11210375708432	39.0	37.0	39.0	34.0	39.0
12	37.06650762356228	39.0	37.0	39.0	33.0	39.0
13	37.10649501548198	39.0	37.0	39.0	33.0	39.0
14	38.210987571152216	40.0	38.0	41.0	33.0	41.0
15	38.20083930260011	40.0	38.0	41.0	33.0	41.0
16	38.065237544885385	40.0	37.0	41.0	33.0	41.0
17	37.96495077286296	40.0	37.0	41.0	33.0	41.0
18	37.73450411307717	39.0	37.0	41.0	33.0	41.0
19	37.45633216111149	39.0	36.0	41.0	33.0	41.0
20	37.43460794432667	39.0	35.0	41.0	32.0	41.0
21	37.43685144096761	39.0	35.0	41.0	33.0	41.0
22	37.5823944227786	39.0	35.0	41.0	33.0	41.0
23	37.520370702282435	39.0	35.0	41.0	33.0	41.0
24	37.41299806552494	39.0	35.0	41.0	33.0	41.0
25	37.12005797244764	39.0	35.0	41.0	32.0	41.0
26	37.2619668605262	39.0	35.0	41.0	33.0	41.0
27	37.27405269435912	39.0	35.0	41.0	33.0	41.0
28	37.13095098299763	39.0	35.0	41.0	32.0	41.0
29	37.139483068707854	39.0	35.0	41.0	32.0	41.0
30	36.84172532926249	39.0	35.0	41.0	31.0	41.0
31	36.726074004486996	39.0	35.0	41.0	31.0	41.0
32	36.414570985346195	39.0	35.0	41.0	30.0	41.0
33	36.10156303113084	39.0	35.0	41.0	29.0	41.0
34	35.83867837652425	39.0	35.0	41.0	27.0	41.0
35	35.628682764630625	39.0	35.0	41.0	25.0	41.0
36	35.46810588315276	39.0	35.0	41.0	24.0	41.0
37	35.39469780780094	39.0	35.0	41.0	23.0	41.0
38	35.19875340696287	39.0	35.0	41.0	23.0	41.0
39	35.08057428569663	39.0	35.0	41.0	23.0	41.0
40	35.017261945229016	38.0	35.0	40.0	23.0	41.0
41	34.850365572524275	38.0	34.0	40.0	22.0	41.0
42	34.81316864543482	38.0	35.0	40.0	22.0	41.0
43	34.84232174090395	38.0	35.0	40.0	22.0	41.0
44	34.960133126494895	38.0	35.0	40.0	22.0	41.0
45	34.9082082310987	38.0	35.0	40.0	23.0	41.0
46	34.79145060908153	38.0	34.0	40.0	23.0	41.0
47	34.60579971693624	38.0	34.0	40.0	22.0	41.0
48	34.55484823950408	37.0	34.0	40.0	22.0	41.0
49	34.45059363044728	37.0	34.0	40.0	22.0	41.0
50	34.320096909166196	37.0	34.0	40.0	22.0	41.0
51	33.44859117063553	36.0	33.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	10.0
10	13.0
11	10.0
12	12.0
13	16.0
14	23.0
15	27.0
16	53.0
17	89.0
18	134.0
19	348.0
20	651.0
21	1225.0
22	1941.0
23	2409.0
24	3112.0
25	4226.0
26	5849.0
27	6410.0
28	5810.0
29	5557.0
30	5861.0
31	6832.0
32	8342.0
33	11114.0
34	17971.0
35	23640.0
36	27303.0
37	37754.0
38	65172.0
39	81649.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77817195196569	17.49093021674773	20.857102242878597	19.873795588407983
2	33.681188620589495	31.308211939357605	20.65283898121767	14.357760458835237
3	25.734080753518214	29.738382333854553	30.180283187372144	14.347253725255097
4	24.76931539360078	20.93312155054666	38.5195394342433	15.778023621609261
5	29.007546306883146	23.92846768561381	28.390121198262065	18.673864809240982
6	22.612035772337624	35.242983665119496	28.885791805983896	13.25918875655898
7	66.82128046180185	5.425182786262137	23.25109239127076	4.502444360665262
8	65.63432858882207	11.828727881780706	17.008547536789017	5.528395992608203
9	59.92793616850328	7.154158503346704	18.31570880278861	14.6021965253614
10	37.022329899073554	21.413650101050056	25.473266543470064	16.090753456406325
11	29.753524391072983	20.54715360226451	30.299565515664305	19.3997564909982
12	23.204430133311906	18.382148441604194	35.00071074962454	23.412710675459362
13	23.597505577839446	20.254201148324174	38.85173762832121	17.29655564551517
14	19.200746596127342	27.276407438767375	32.624952874209676	20.897893090895607
15	17.113614872590404	22.04158194325128	39.985537790248515	20.859265393909805
16	18.797164418019666	22.813826861391462	33.21518408415275	25.173824636436116
17	19.106804037057866	25.368199207668678	33.441078856125735	22.083917899147718
18	19.82280702838672	23.276432160493446	32.89843696886916	24.002323842250668
19	20.10834296450578	24.543111600051915	30.57243156717202	24.776113868270283
20	22.82464261654749	24.517153787677458	33.69478556992849	18.963418025846565
21	20.70815384330134	29.18956001508025	31.132996705830003	18.969289435788404
22	19.7891236766151	22.323718642035587	33.597752795100156	24.28940488624916
23	22.010679785662635	25.718320653148	31.83911100673049	20.431888554458872
24	21.941458952664075	24.521480089739867	30.47292661973659	23.064134337859468
25	19.584551393378284	30.565324070926632	28.56966273385208	21.280461801843003
26	19.569100314583963	24.319070957534255	32.779154640577005	23.332674087304774
27	24.97450571998937	23.981928418242163	28.883628654952687	22.15993720681578
28	20.59721509755811	25.399719408409094	32.403693425875	21.5993720681578
29	24.34904605039524	22.19949196852924	30.64227044332235	22.809191537753165
30	21.697640929289683	27.456567017509165	28.563173280758463	22.282618772442692
31	25.23470188688574	23.412710675459362	26.595632907089573	24.756954530565324
32	25.645391561238807	27.812868894506217	25.744587487098347	20.79715205715663
33	22.47112193373341	27.756626967694885	25.57215344775372	24.20009765081798
34	23.839778493334403	24.79372809809581	28.505386246067697	22.861107162502087
35	22.44485509978307	24.54465670793135	26.046501566739387	26.963986625546195
36	23.894166290690418	29.739000377006324	25.117273688048904	21.24955964425436
37	20.553643055358126	27.498902973405603	28.905878208416514	23.04157576281976
38	21.263465615169252	28.537833511535776	25.519928801428915	24.678772071866057
39	22.084535942299492	28.881774525497374	28.62126933702511	20.412420195178026
40	20.452283978467374	26.395386925915165	29.909580286895633	23.242748808721824
41	19.123491202155734	27.57059597901125	28.922874395090265	24.383038423742747
42	20.485040265511337	25.31721064764742	30.106736052311174	24.09101303453007
43	22.861416184077974	26.553296951193133	27.077397543896513	23.50788932083238
44	22.63861162786386	24.078652171494614	29.259089869654698	24.023646330986832
45	21.47823561041032	22.99645861274034	28.35396567388335	27.171340102965992
46	25.225431239609147	26.68957546615905	26.666707869543448	21.418285424688353
47	19.64233842806905	25.18834865050278	33.63452636263064	21.534786558797535
48	21.178175660224596	24.8508970896348	29.13084591566183	24.84008133447877
49	20.342272297451807	23.010364583655228	33.17130302037688	23.476060098516076
50	20.745854475559483	23.133664192433915	31.729717368866694	24.390763963139904
51	20.85772028603037	22.341332871861113	28.822751404503062	27.978195437605454
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	124.0
1	298.5
2	473.0
3	4705.5
4	8938.0
5	5783.0
6	2628.0
7	2594.0
8	2560.0
9	2524.0
10	2488.0
11	2493.5
12	2499.0
13	2438.5
14	2378.0
15	2230.0
16	2082.0
17	1994.0
18	1906.0
19	1901.5
20	1897.0
21	1821.5
22	1746.0
23	1645.0
24	1544.0
25	1754.0
26	2073.0
27	2182.0
28	2787.5
29	3393.0
30	3861.0
31	4329.0
32	4923.5
33	5518.0
34	6028.5
35	6539.0
36	6797.5
37	7056.0
38	7623.5
39	8191.0
40	9179.5
41	10168.0
42	11607.0
43	13046.0
44	14527.0
45	16008.0
46	29914.5
47	43821.0
48	36434.5
49	29048.0
50	28368.5
51	27689.0
52	24449.5
53	21210.0
54	19152.0
55	17094.0
56	15728.0
57	14362.0
58	13596.5
59	12831.0
60	12125.0
61	11419.0
62	10954.0
63	10489.0
64	9199.0
65	7909.0
66	6808.5
67	5708.0
68	4935.5
69	4163.0
70	3505.5
71	2848.0
72	2511.5
73	2175.0
74	1802.0
75	1130.0
76	831.0
77	665.5
78	500.0
79	350.0
80	200.0
81	161.5
82	123.0
83	92.0
84	61.0
85	43.5
86	26.0
87	16.0
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	323602.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.08933276302805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49388544523647	32.09891818760687
2	8.66412807644396	7.4666299557477585
3	4.097014031046394	5.296128027556589
4	2.748542484352164	4.737314468862809
5	2.1061584712514287	4.5376481609711625
6	1.5531715269680673	4.015507485815247
7	1.3037691293768332	3.932497930331722
8	1.1055660284847935	3.8110481994304486
9	0.8676305115461946	3.3647057844633204
>10	2.9897541731629054	17.5708593360034
>50	0.03590822557693244	1.0521378069537357
>100	0.025853922415391358	2.3933040585235856
>500	0.003590822557693245	1.2588519407905283
>1k	0.003590822557693245	4.487367746657682
>5k	0.001436329023077298	3.9770809102851206
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	6710	2.073534774197934	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6142	1.8980105190944432	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC	4996	1.5438717931285963	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG	4976	1.5376913616108676	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1792	0.5537666639884797	No Hit
GAACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCT	1569	0.4848548525658062	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCT	1168	0.3609372006353484	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTC	925	0.2858449576949462	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGAATCAT	887	0.27410213781126197	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCGAATCATCGTA	771	0.2382556350084363	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT	743	0.2296030308836163	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCC	742	0.22929400930772986	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	469	0.14493111909073492	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG	434	0.13411536393470994	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	427	0.13195221290350492	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCAACCATCGAATGC	376	0.11619211253329707	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT	352	0.10877559471202279	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0902157588642837E-4	0.0	0.0	0.07972756657869852	0.0
2	3.0902157588642837E-4	0.0	0.0	0.9029610447401437	0.0
3	3.0902157588642837E-4	0.0	0.0	1.2972725755712264	0.0
4	3.0902157588642837E-4	0.0	0.0	2.199615577159597	0.0
5	3.0902157588642837E-4	0.0	0.0	5.312080889487704	0.0
6	3.0902157588642837E-4	0.0	0.0	6.632530083250413	0.0
7	3.0902157588642837E-4	0.0	0.0	7.797232402766361	0.0
8	3.0902157588642837E-4	0.0	0.0	9.272810427624057	0.0
9	3.0902157588642837E-4	0.0	0.0	9.953893980877744	0.0
10	3.0902157588642837E-4	0.0	0.0	12.589539001613092	0.0
11	3.0902157588642837E-4	0.0	0.0	13.861780829537519	0.0
12	3.0902157588642837E-4	0.0	0.0	16.4056464422346	0.0
13	3.0902157588642837E-4	0.0	0.0	16.94797930791528	0.0
14	3.0902157588642837E-4	0.0	0.0	17.196741676503855	0.0
15	3.0902157588642837E-4	0.0	0.0	17.701991953078164	0.0
16	6.180431517728567E-4	0.0	0.0	18.219603092687933	0.0
17	6.180431517728567E-4	0.0	0.0	18.809525281055123	0.0
18	6.180431517728567E-4	0.0	0.0	19.44240146847053	0.0
19	6.180431517728567E-4	0.0	0.0	20.137700014214992	0.0
20	6.180431517728567E-4	0.0	0.0	20.571257285183652	0.0
21	6.180431517728567E-4	0.0	0.0	20.980092830081396	0.0
22	6.180431517728567E-4	0.0	0.0	21.440534978152176	0.0
23	6.180431517728567E-4	0.0	0.0	21.8339194442556	0.0
24	6.180431517728567E-4	0.0	0.0	22.170752961971804	0.0
25	6.180431517728567E-4	0.0	0.0	22.480701602585892	0.0
26	6.180431517728567E-4	0.0	0.0	22.761602215066656	0.0
27	6.180431517728567E-4	0.0	0.0	23.095654538599884	0.0
28	6.180431517728567E-4	0.0	0.0	23.370683741138805	0.0
29	6.180431517728567E-4	0.0	0.0	23.667653475565665	0.0
30	6.180431517728567E-4	0.0	0.0	24.02704556832158	0.0
31	6.180431517728567E-4	0.0	0.0	24.347500942515808	0.0
32	6.180431517728567E-4	0.0	0.0	24.635818072817845	0.0
33	6.180431517728567E-4	0.0	0.0	24.92351715996811	0.0
34	6.180431517728567E-4	0.0	0.0	25.211216247118372	0.0
35	6.180431517728567E-4	0.0	0.0	25.49458903220623	0.0
36	6.180431517728567E-4	0.0	0.0	25.769000191593378	0.0
37	6.180431517728567E-4	0.0	0.0	26.067515033899667	0.0
38	6.180431517728567E-4	0.0	0.0	26.345325430621568	0.0
39	6.180431517728567E-4	0.0	0.0	26.63580571195481	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATTA	20	7.0257665E-4	45.000004	8
TCGAATA	20	7.0257665E-4	45.000004	44
GCGTAAG	20	7.0257665E-4	45.000004	1
AAACGAT	35	1.2083183E-7	45.0	16
CTACGAA	50	2.1827873E-11	45.0	11
CTCGAGG	30	2.160652E-6	44.999996	2
CCGCGAA	65	0.0	44.999996	41
CATCGAA	175	0.0	43.714287	42
ATCGAAT	175	0.0	43.714287	43
CGTTTTT	2745	0.0	42.95082	1
TCGAATG	160	0.0	42.187504	44
CCGATGA	715	0.0	42.16783	18
CTCATGC	75	0.0	42.0	23
GATGAAT	800	0.0	41.90625	20
ACCCGCG	70	0.0	41.785713	39
CCCGCGA	70	0.0	41.785713	40
CACCCGC	65	0.0	41.53846	38
CGATGAA	775	0.0	41.516125	19
TACGAAT	55	6.002665E-11	40.909092	12
ATGAATG	805	0.0	40.807453	21
>>END_MODULE