FastQCFastQC Report
Sat 18 Jun 2016
SRR3551615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530504
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC107742.0308989187640436No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84431.5915054363397827No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC79881.5057379397704824No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG51030.9619154615233815No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC29310.5524934779002608TruSeq Adapter, Index 15 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT28020.5281769788729208No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT18660.3517409859303606No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAGGTAT15880.2993379880264805No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT15460.2914209883431605No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC14120.2661619893535204No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTA11290.21281649148734033No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC8940.16851899325924027TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC7110.13402349463906021No Hit
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT7000.1319499947220002TruSeq Adapter, Index 21 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG6830.1287454948501802TruSeq Adapter, Index 21 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC6010.11328849546846018No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC5590.10537149578514018No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5490.10348649586054015No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT5340.10065899597364017No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC207.0298475E-445.00000443
CGCGTTT253.8879552E-545.00000414
AGTAACG207.0298475E-445.0000041
TATTAGT900.045.030
CGTAAGC351.2102828E-745.043
TCGAATA302.163284E-644.99999644
CGTTTTT36600.042.9713131
GATGAAT11700.042.30769320
CGATGAA10700.042.26635419
CCGATGA10900.040.6651418
GAATGCG501.0786607E-940.5000041
CCGATCG1000.040.50000440
CGTAAGG850.039.7058832
TACGGCT8850.039.1525467
CGTTAGG750.039.0000042
ATGAATG12600.038.92857421
ATTAGTC1000.038.25000431
ATATTAG1000.038.25000429
GCGTTAG301.1390935E-437.4999961
TACGCGG301.1390935E-437.4999962
Produced by FastQC (version 0.11.5)