##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551615_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	530504
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9369599475216	33.0	31.0	34.0	30.0	34.0
2	32.04317026827319	33.0	31.0	34.0	30.0	34.0
3	31.99692556512298	33.0	31.0	34.0	30.0	34.0
4	35.649261834029524	37.0	35.0	37.0	33.0	37.0
5	35.665197623392096	37.0	35.0	37.0	35.0	37.0
6	35.547905388083784	37.0	35.0	37.0	33.0	37.0
7	35.819475442220984	37.0	35.0	37.0	35.0	37.0
8	35.52133819914647	37.0	35.0	37.0	33.0	37.0
9	37.27379058404838	39.0	37.0	39.0	34.0	39.0
10	37.073622442055104	39.0	37.0	39.0	33.0	39.0
11	37.15566140877355	39.0	37.0	39.0	34.0	39.0
12	37.12134687014613	39.0	37.0	39.0	34.0	39.0
13	37.17105808815768	39.0	37.0	39.0	33.0	39.0
14	38.278297618868095	40.0	38.0	41.0	33.0	41.0
15	38.249828465006864	40.0	38.0	41.0	33.0	41.0
16	38.10329799586808	40.0	37.0	41.0	33.0	41.0
17	38.00361354485546	40.0	37.0	41.0	33.0	41.0
18	37.784644413614224	39.0	37.0	41.0	33.0	41.0
19	37.56173186253073	39.0	37.0	41.0	33.0	41.0
20	37.586035166558595	39.0	35.0	41.0	33.0	41.0
21	37.55891567264337	39.0	35.0	41.0	33.0	41.0
22	37.6758874579645	40.0	35.0	41.0	33.0	41.0
23	37.6717800431288	40.0	35.0	41.0	33.0	41.0
24	37.597750441089985	40.0	35.0	41.0	33.0	41.0
25	37.298122163075114	39.0	35.0	41.0	33.0	41.0
26	37.42323337807066	39.0	35.0	41.0	33.0	41.0
27	37.4124926485003	39.0	35.0	41.0	33.0	41.0
28	37.23817728047291	39.0	35.0	41.0	32.0	41.0
29	37.26318180447273	39.0	35.0	41.0	33.0	41.0
30	37.00565122977395	39.0	35.0	41.0	32.0	41.0
31	36.94709936211603	39.0	35.0	41.0	31.0	41.0
32	36.68373659765054	39.0	35.0	41.0	31.0	41.0
33	36.491085835356564	39.0	35.0	41.0	30.0	41.0
34	36.162339963506405	39.0	35.0	41.0	29.0	41.0
35	35.901555878937764	39.0	35.0	41.0	27.0	41.0
36	35.78908547343658	39.0	35.0	41.0	27.0	41.0
37	35.68556316257747	39.0	35.0	41.0	27.0	41.0
38	35.644147829234086	39.0	35.0	41.0	26.0	41.0
39	35.50628270474869	39.0	35.0	41.0	26.0	41.0
40	35.40542766878289	38.0	35.0	40.0	26.0	41.0
41	35.20733679670653	38.0	35.0	40.0	24.0	41.0
42	35.064985372400585	38.0	35.0	40.0	23.0	41.0
43	35.173614148055435	38.0	35.0	40.0	24.0	41.0
44	35.3529850858806	38.0	35.0	40.0	26.0	41.0
45	35.35534133578635	38.0	35.0	40.0	26.0	41.0
46	35.283882873644686	38.0	35.0	40.0	26.0	41.0
47	35.10569571577217	38.0	35.0	40.0	25.0	41.0
48	35.07704183191833	38.0	35.0	40.0	25.0	41.0
49	35.047356853105725	38.0	35.0	40.0	26.0	41.0
50	34.90650777373969	37.0	34.0	40.0	24.0	41.0
51	33.98376638064934	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	17.0
10	16.0
11	23.0
12	22.0
13	19.0
14	28.0
15	37.0
16	65.0
17	110.0
18	218.0
19	454.0
20	950.0
21	1933.0
22	2935.0
23	3777.0
24	4611.0
25	5864.0
26	7463.0
27	8546.0
28	8453.0
29	8236.0
30	8965.0
31	10619.0
32	13271.0
33	17791.0
34	30495.0
35	39483.0
36	43687.0
37	63301.0
38	108688.0
39	140372.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01813369927466	16.338048346478065	20.20757619169695	18.436241762550328
2	31.71305023147799	31.32360924705563	21.795688628172456	15.167651893293923
3	27.790553888377843	30.03389229864431	27.961900381523986	14.213653431453862
4	25.756073469757062	21.793049628278016	36.13639105454436	16.314485847420563
5	29.213540331458386	25.58774297649028	26.328924946843003	18.86979174520833
6	23.94609654215614	36.14977455400902	26.291601948335924	13.612526955498922
7	68.91503174339873	5.773377769064889	20.551400177943993	4.760190309592387
8	68.3732827650687	11.346380046144798	14.727692910892284	5.552644277894228
9	62.18181201272752	7.337362206505511	16.137672854493086	14.343152926273884
10	39.79743790810248	21.33857614645695	23.238656070453757	15.625329874986804
11	30.837656266493752	21.498989640040413	28.037300378507986	19.626053714957852
12	23.89388204424472	18.83906624643735	33.07986367680545	24.18718803251248
13	24.98454300061828	20.36648168534073	36.38728454450862	18.26169076953237
14	19.928407702863694	27.711760891529565	31.27327974906881	21.08655165653793
15	18.398353264065868	21.640930134362797	37.57671949693122	22.383997104640116
16	20.61190867552365	22.392102604315898	31.28854824845807	25.70744047170238
17	20.401165683953373	25.554189977832404	31.11531675538733	22.929327582826897
18	20.879578664816854	23.196620572135178	30.296284288148627	25.62751647489934
19	21.756103629755856	23.75458054981678	28.649925354002985	25.839390466424383
20	24.82412950703482	23.742139550314416	31.33623874655045	20.097492196100312
21	22.526126098954958	27.881976384720947	29.63936181442553	19.952535701898572
22	20.615867175365313	21.722927631082893	32.55621069775157	25.10499449580022
23	23.471453561141857	24.56324551747018	30.22936679082533	21.735934130562633
24	23.28615806855368	24.30405802783768	28.977915340883385	23.43186856272526
25	21.053375657864972	28.822402847103884	27.504976399800945	22.619245095230195
26	21.399650144013993	23.135735074570597	30.701747771930087	24.76286700948532
27	25.811869467525224	24.018669039253236	27.316287907348485	22.85317358587306
28	21.001915159923392	25.22261849109526	30.283843288646267	23.49162306033508
29	24.4797400208104	23.241483570340655	29.011091339556344	23.267685069292597
30	23.143463574261457	28.037111878515525	27.860487385580505	20.958937161642513
31	24.560983517560658	23.823006047079758	24.70480901180764	26.911201423551944
32	26.081612956735484	28.083294376668228	25.10763349569466	20.727459170901632
33	26.812238927510446	25.4458024821679	24.113861535445537	23.628097054876118
34	27.22788141088474	23.80641804774328	25.520259979189603	23.445440562182377
35	25.18020599279176	25.354191485832338	27.288201408471945	22.177401112903954
36	25.07785049688598	29.142475834300967	24.6955725121771	21.084101156635953
37	22.375703104971876	29.071411337143548	27.537398398504067	21.015487159380513
38	22.71179859152806	25.9566374617345	25.7464599701416	25.585103976595843
39	26.031094958756203	27.164168413433266	25.758712469651503	21.04602415815903
40	24.57851401685944	23.516128059354877	29.85425180582993	22.051106117955754
41	22.169672613213095	27.03504591859816	26.739854930405805	24.05542653778294
42	22.373818105047274	24.91536350338546	30.76960776921569	21.941210622351576
43	24.28728152850874	25.31799948728002	27.088956916441724	23.305762067769518
44	23.44883356204666	23.91631354334746	28.423348363066065	24.211504531539816
45	22.917452083301917	22.249031110038757	27.733815390647383	27.099701416011946
46	26.11761645529534	25.32252348709906	26.922699923092004	21.637160134513593
47	21.471091641156335	24.832235006710597	32.06629921734803	21.630374134785036
48	22.070144617194217	24.6013225159471	28.374715365011387	24.9538175018473
49	21.671090133156394	22.654306093827756	32.959789181608436	22.714814591407418
50	22.567973097281076	22.779093088836277	31.012961259481546	23.639972554401098
51	22.314629107414834	22.322546107098155	28.456524361739028	26.906300423747982
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	422.0
2	714.0
3	6745.5
4	12777.0
5	8160.5
6	3544.0
7	3374.5
8	3205.0
9	3110.5
10	3016.0
11	2987.0
12	2958.0
13	2866.0
14	2774.0
15	2553.0
16	2332.0
17	2201.0
18	2070.0
19	1967.0
20	1864.0
21	1850.5
22	1837.0
23	1929.5
24	2022.0
25	2350.0
26	3111.0
27	3544.0
28	3950.0
29	4356.0
30	5155.0
31	5954.0
32	6942.5
33	7931.0
34	8950.5
35	9970.0
36	10851.0
37	11732.0
38	12438.0
39	13144.0
40	14591.0
41	16038.0
42	17425.5
43	18813.0
44	21877.5
45	24942.0
46	45920.5
47	66899.0
48	56487.5
49	46076.0
50	46072.0
51	46068.0
52	40640.5
53	35213.0
54	33065.5
55	30918.0
56	28999.0
57	27080.0
58	25663.0
59	24246.0
60	23676.0
61	23106.0
62	21867.5
63	20629.0
64	18572.0
65	16515.0
66	13990.0
67	11465.0
68	9699.5
69	7934.0
70	6986.0
71	6038.0
72	5130.0
73	4222.0
74	3398.0
75	2060.0
76	1546.0
77	1212.5
78	879.0
79	677.0
80	475.0
81	333.5
82	192.0
83	116.5
84	41.0
85	30.0
86	19.0
87	16.5
88	14.0
89	8.5
90	3.0
91	3.0
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	530504.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.59109323756351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75720480146468	26.62265664594898
2	7.772312812105719	5.6879484555853965
3	3.650641050645879	4.007428411831804
4	2.501798381261064	3.6617415132123625
5	2.107383006702946	3.855572404276221
6	1.662231376727863	3.6493717972951667
7	1.5738245173145378	4.031157175683375
8	1.329316579643678	3.891291752638467
9	1.1802967732318723	3.8869514349590517
>10	5.371912354633566	27.618378537393877
>50	0.05015888660011768	1.2332919441992636
>100	0.03309452311757348	2.239717205197374
>500	0.004136815389696685	0.9897744952595643
>1k	0.0036197134659845995	2.51161664119202
>5k	0.001551305771136257	4.074518061731879
>10k+	5.171019237120856E-4	2.0385835235952103
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	10774	2.0308989187640436	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8443	1.5915054363397827	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC	7988	1.5057379397704824	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG	5103	0.9619154615233815	No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	2931	0.5524934779002608	TruSeq Adapter, Index 15 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	2802	0.5281769788729208	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT	1866	0.3517409859303606	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAGGTAT	1588	0.2993379880264805	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT	1546	0.2914209883431605	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC	1412	0.2661619893535204	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTA	1129	0.21281649148734033	No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	894	0.16851899325924027	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC	711	0.13402349463906021	No Hit
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	700	0.1319499947220002	TruSeq Adapter, Index 21 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	683	0.1287454948501802	TruSeq Adapter, Index 21 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	601	0.11328849546846018	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	559	0.10537149578514018	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	549	0.10348649586054015	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT	534	0.10065899597364017	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1379819944807202	0.0
2	0.0	0.0	0.0	0.9647429614102816	0.0
3	0.0	0.0	0.0	1.292167448313302	0.0
4	0.0	0.0	0.0	2.1594559136217635	0.0
5	0.0	0.0	0.0	5.065748797370048	0.0
6	0.0	0.0	0.0	6.33001824679927	0.0
7	0.0	0.0	0.0	7.359605205615792	0.0
8	0.0	0.0	0.0	8.721894651124215	0.0
9	0.0	0.0	0.0	9.329430126822794	0.0
10	0.0	0.0	0.0	11.192752552289898	0.0
11	0.0	0.0	0.0	12.1814350127426	0.0
12	0.0	0.0	0.0	14.531087418756503	0.0
13	0.0	0.0	0.0	15.027030898918763	0.0
14	0.0	0.0	0.0	15.263032889478685	0.0
15	0.0	0.0	0.0	15.720522371179106	0.0
16	0.0	0.0	0.0	16.154826353806946	0.0
17	0.0	0.0	0.0	16.605341335786346	0.0
18	0.0	0.0	0.0	17.107128315714867	0.0
19	0.0	0.0	0.0	17.74256179029753	0.0
20	0.0	0.0	0.0	18.11164477553421	0.0
21	0.0	0.0	0.0	18.46451676141933	0.0
22	1.884999924600003E-4	0.0	0.0	18.86206324551747	0.0
23	1.884999924600003E-4	0.0	0.0	19.222286731108532	0.0
24	1.884999924600003E-4	0.0	0.0	19.53444271862229	0.0
25	1.884999924600003E-4	0.0	0.0	19.81662720733491	0.0
26	1.884999924600003E-4	0.0	0.0	20.134438194622472	0.0
27	3.769999849200006E-4	0.0	0.0	20.45451118181955	0.0
28	3.769999849200006E-4	0.0	0.0	20.743293170268274	0.0
29	3.769999849200006E-4	0.0	0.0	21.010209159591632	0.0
30	3.769999849200006E-4	0.0	0.0	21.329905146803796	0.0
31	3.769999849200006E-4	0.0	0.0	21.627169634913216	0.0
32	3.769999849200006E-4	0.0	0.0	21.929523622819055	0.0
33	3.769999849200006E-4	0.0	0.0	22.237155610513774	0.0
34	5.65499977380001E-4	0.0	0.0	22.523298599068056	0.0
35	5.65499977380001E-4	0.0	0.0	22.813777087448916	0.0
36	5.65499977380001E-4	0.0	0.0	23.09068357637266	0.0
37	5.65499977380001E-4	0.0	0.0	23.361935065522598	0.0
38	5.65499977380001E-4	0.0	0.0	23.642611554295538	0.0
39	5.65499977380001E-4	0.0	0.0	23.940441542382338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATC	20	7.0298475E-4	45.000004	43
CGCGTTT	25	3.8879552E-5	45.000004	14
AGTAACG	20	7.0298475E-4	45.000004	1
TATTAGT	90	0.0	45.0	30
CGTAAGC	35	1.2102828E-7	45.0	43
TCGAATA	30	2.163284E-6	44.999996	44
CGTTTTT	3660	0.0	42.971313	1
GATGAAT	1170	0.0	42.307693	20
CGATGAA	1070	0.0	42.266354	19
CCGATGA	1090	0.0	40.66514	18
GAATGCG	50	1.0786607E-9	40.500004	1
CCGATCG	100	0.0	40.500004	40
CGTAAGG	85	0.0	39.705883	2
TACGGCT	885	0.0	39.152546	7
CGTTAGG	75	0.0	39.000004	2
ATGAATG	1260	0.0	38.928574	21
ATTAGTC	100	0.0	38.250004	31
ATATTAG	100	0.0	38.250004	29
GCGTTAG	30	1.1390935E-4	37.499996	1
TACGCGG	30	1.1390935E-4	37.499996	2
>>END_MODULE