FastQCFastQC Report
Sat 18 Jun 2016
SRR3551611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426428
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC69891.6389636703030757No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG65841.5439886686615325No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC61581.4440890373052426No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28920.6781918635736865No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC22740.5332670462539983No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT14350.33651636384102357No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT14290.33510932677966737No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTT11710.274606733141351No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC11250.2638194490042868No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT10900.25561173281304234No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTA10690.2506871030982956No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT6690.15688463234121586Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC6430.15078747174200569No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC5550.13015092817544816No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5480.12850938493719924No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA207.0282904E-445.01
CCGATCG502.1827873E-1145.040
AACCCCG253.8866645E-545.036
CTAAGCG207.0282904E-445.01
AGTATCG207.0282904E-445.01
GTTACGG207.0282904E-445.02
TACGAAT502.1827873E-1145.012
CGCAACT253.8866645E-545.043
CGTCATA406.7975634E-945.038
ATCGATC207.0282904E-445.029
CGAACCC302.1622782E-644.99999634
CGTTTTT13650.041.7032971
ACAACGA603.6379788E-1241.24999613
ACGGCTG7400.041.04738
GATGAAT7400.041.047320
CTACGAA556.002665E-1140.90909211
CGAATAT556.002665E-1140.90909214
GCTACGA556.002665E-1140.90909210
CCGATGA7050.040.85106318
CGATGAA7000.040.82142619