##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551611_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 426428 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94171348973332 33.0 31.0 34.0 30.0 34.0 2 32.02096016209067 33.0 31.0 34.0 30.0 34.0 3 32.00970386559982 33.0 31.0 34.0 30.0 34.0 4 35.68893693659891 37.0 35.0 37.0 33.0 37.0 5 35.69003442550677 37.0 35.0 37.0 35.0 37.0 6 35.580194077311994 37.0 35.0 37.0 33.0 37.0 7 35.85547149811926 37.0 35.0 37.0 35.0 37.0 8 35.55680443122872 37.0 35.0 37.0 33.0 37.0 9 37.30678098061103 39.0 37.0 39.0 34.0 39.0 10 37.06884163328862 39.0 37.0 39.0 33.0 39.0 11 37.16277777256653 39.0 37.0 39.0 34.0 39.0 12 37.09885607886912 39.0 37.0 39.0 34.0 39.0 13 37.12255761816766 39.0 37.0 39.0 33.0 39.0 14 38.20667029369554 40.0 37.0 41.0 33.0 41.0 15 38.18214798277786 40.0 37.0 41.0 33.0 41.0 16 38.05923391522133 40.0 37.0 41.0 33.0 41.0 17 37.99124353935483 40.0 37.0 41.0 33.0 41.0 18 37.82295487163132 39.0 37.0 41.0 33.0 41.0 19 37.67121295974936 39.0 37.0 41.0 33.0 41.0 20 37.7822938456199 40.0 36.0 41.0 33.0 41.0 21 37.7537356833979 40.0 36.0 41.0 33.0 41.0 22 37.89386015927659 40.0 36.0 41.0 33.0 41.0 23 37.87354723423415 40.0 36.0 41.0 33.0 41.0 24 37.78368915737241 40.0 36.0 41.0 33.0 41.0 25 37.46981670997214 39.0 35.0 41.0 33.0 41.0 26 37.5897408237733 39.0 36.0 41.0 33.0 41.0 27 37.62220116877879 40.0 36.0 41.0 33.0 41.0 28 37.48729914545949 40.0 36.0 41.0 33.0 41.0 29 37.50624489949065 40.0 36.0 41.0 33.0 41.0 30 37.2966550038928 40.0 35.0 41.0 32.0 41.0 31 37.28374778391663 39.0 35.0 41.0 32.0 41.0 32 37.16929704428415 40.0 35.0 41.0 32.0 41.0 33 37.07051600739163 40.0 35.0 41.0 32.0 41.0 34 36.89571275807405 40.0 35.0 41.0 31.0 41.0 35 36.883703696755376 40.0 35.0 41.0 31.0 41.0 36 36.68354798465391 39.0 35.0 41.0 31.0 41.0 37 36.63231776524994 39.0 35.0 41.0 31.0 41.0 38 36.59711135291304 39.0 35.0 41.0 31.0 41.0 39 36.41936270601368 39.0 35.0 41.0 30.0 41.0 40 36.39993621431989 39.0 35.0 41.0 30.0 41.0 41 36.15176301743788 39.0 35.0 41.0 30.0 41.0 42 36.20244449238793 39.0 35.0 41.0 30.0 41.0 43 36.13553049987337 39.0 35.0 41.0 30.0 41.0 44 36.27254776890823 39.0 35.0 41.0 30.0 41.0 45 36.25969214029097 39.0 35.0 41.0 30.0 41.0 46 36.154745936007956 39.0 35.0 41.0 30.0 41.0 47 36.01984625775043 38.0 35.0 41.0 30.0 41.0 48 35.99455242151078 38.0 35.0 40.0 30.0 41.0 49 35.97950416013958 38.0 35.0 40.0 30.0 41.0 50 35.823606798803084 38.0 35.0 40.0 30.0 41.0 51 35.042588666785484 37.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 5.0 9 12.0 10 18.0 11 19.0 12 10.0 13 14.0 14 19.0 15 32.0 16 34.0 17 47.0 18 133.0 19 300.0 20 622.0 21 1185.0 22 1868.0 23 2538.0 24 2938.0 25 3463.0 26 3969.0 27 4562.0 28 4695.0 29 5100.0 30 6021.0 31 7636.0 32 10169.0 33 14094.0 34 24816.0 35 32041.0 36 34507.0 37 50820.0 38 87344.0 39 127353.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.886527151125165 17.8529552468412 21.78398229009352 14.476535311940117 2 29.432635755625803 31.924029378933845 22.782040578948852 15.861294286491507 3 29.163891676906772 30.59179978800642 25.380134512743062 14.864174022343748 4 26.289549466732954 22.89647959327249 33.10547149811926 17.7084994418753 5 30.331966944009302 26.57330194077312 23.493063307287514 19.601667807930063 6 24.77581209489058 37.11458909827685 23.81433676963051 14.295262037202061 7 72.12894087630268 5.772369544213794 17.156471901469885 4.942217678013639 8 71.35202191225717 11.611573348842008 11.119579389721125 5.916825349179698 9 65.28370557280479 7.57384599510351 12.775427504760476 14.367020927331225 10 40.441528229853574 23.043045953830426 19.5972121905691 16.9182136257469 11 31.728216721228435 22.2504150759331 25.32666710441153 20.694701098426933 12 25.86673482979542 19.176508109223597 30.67739454257225 24.279362518408735 13 25.585092911347285 21.052792030542083 33.96306058701587 19.399054471094768 14 21.244852589417203 28.134878572701606 28.349686230735315 22.270582607145872 15 18.202603956588217 23.237686080651365 35.86162259513916 22.698087367621262 16 20.363109364300655 22.572157550629885 29.909152307071768 27.15558077799769 17 20.477548378624295 25.01758796326695 29.79096119391785 24.713902464190905 18 20.77349517386288 23.68582738469331 29.74499798324688 25.79567945819693 19 22.772894838050036 24.52231091766957 26.700169782472074 26.004624461808323 20 25.411323834269794 23.959496093127093 29.36087686549664 21.268303207106477 21 23.14106953577157 28.57317061731406 27.19333627247742 21.09242357443695 22 21.818689204273642 22.10947686362059 29.189687356364967 26.882146575740805 23 23.36056731734314 26.263519281097864 27.137523802376958 23.238389599182042 24 23.195240462633787 24.727738328627577 27.02308478805332 25.053936420685318 25 21.556980310861388 30.171564719014697 24.422880298667067 23.848574671456845 26 20.896376410554655 25.096147532526007 28.857626609884907 25.149849447034434 27 26.103820574633936 24.447503447240802 25.204020373896647 24.244655604228615 28 21.081401784122995 26.24077218193927 28.580440308797733 24.09738572514 29 23.409813614490606 24.752126971024417 27.3481103492266 24.48994906525838 30 24.257318937780823 26.50365360623599 26.335747183580814 22.903280272402373 31 24.741808699241137 23.869914733554083 23.38847355239337 27.99980301481141 32 26.162212612680218 25.845629273875076 25.348710685039443 22.643447428405263 33 23.981539673755005 23.024050953502115 24.50378492969505 28.490624443047828 34 22.734904837393415 25.02438864239684 27.258294483476696 24.98241203673305 35 27.50710553715985 22.227198964420722 25.81303291528699 24.45266258313244 36 22.540264710572476 27.206937630737194 26.067237611038674 24.185560047651656 37 23.982243192285686 23.556614481225434 30.52449651523821 21.936645811250667 38 21.7717879688951 23.802845966962767 25.3308882155956 29.09447784854653 39 23.221036142091982 23.445458553378295 30.29069385687619 23.04281144765353 40 24.11286313281492 22.131051431894715 28.748346731452905 25.007738703837457 41 20.936242460626413 24.1262299848978 29.773138724474002 25.164388830001784 42 23.85162325175645 23.618524111925108 29.686136932846814 22.843715703471627 43 23.906263190972453 22.839025579933775 28.836286547787672 24.418424681306107 44 23.87343232620747 22.06890729501815 28.555817160224002 25.501843218550373 45 22.508840882868856 21.471620062472446 27.80070727062951 28.21883178402919 46 25.825461742662302 24.713433451837123 26.495445890044746 22.965658915455833 47 21.362105677863553 23.395977750053937 32.16744679054847 23.074469781534045 48 22.79235885073213 23.513934357030962 27.92171245790614 25.771994334330767 49 22.650248107535155 21.537985310533077 31.631600176348645 24.180166405583122 50 22.153798531053308 21.86910803230557 30.166874595476845 25.81021884116428 51 22.05483692440459 22.143949271623814 27.1970883713077 28.604125432663896 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 123.0 1 374.0 2 625.0 3 2547.5 4 4470.0 5 2949.0 6 1428.0 7 1339.0 8 1250.0 9 1285.0 10 1320.0 11 1329.5 12 1339.0 13 1282.0 14 1225.0 15 1241.5 16 1258.0 17 1144.5 18 1031.0 19 1048.0 20 1065.0 21 1135.5 22 1206.0 23 1384.0 24 1562.0 25 1808.0 26 2419.0 27 2784.0 28 3290.0 29 3796.0 30 4660.0 31 5524.0 32 6085.5 33 6647.0 34 7297.5 35 7948.0 36 8513.0 37 9078.0 38 9795.0 39 10512.0 40 12209.5 41 13907.0 42 15459.5 43 17012.0 44 19752.0 45 22492.0 46 39149.5 47 55807.0 48 47582.5 49 39358.0 50 38427.5 51 37497.0 52 32761.0 53 28025.0 54 25741.5 55 23458.0 56 22210.5 57 20963.0 58 20813.5 59 20664.0 60 19571.0 61 18478.0 62 17296.5 63 16115.0 64 14470.5 65 12826.0 66 11398.5 67 9971.0 68 8521.5 69 7072.0 70 6318.5 71 5565.0 72 4998.0 73 4431.0 74 3667.0 75 2268.0 76 1633.0 77 1306.5 78 980.0 79 798.0 80 616.0 81 435.0 82 254.0 83 174.5 84 95.0 85 69.5 86 44.0 87 25.0 88 6.0 89 5.5 90 5.0 91 5.5 92 6.0 93 3.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 426428.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.34333093371353 #Duplication Level Percentage of deduplicated Percentage of total 1 72.94726813103533 25.052521701344805 2 7.968933526060426 5.473594425485157 3 3.2818880059954454 3.381328976318603 4 2.1796683486615622 2.994282856952997 5 1.7127815006659233 2.941131094725614 6 1.4195776329062135 2.9251814659797475 7 1.2625487186653261 3.0352089925541494 8 1.1287684261039024 3.1012534084170627 9 1.0624437492887733 3.2839071552251733 >10 6.930596773161585 36.10696739059014 >50 0.06304102107732847 1.5009365766268363 >100 0.032205739028635195 2.0539627533394524 >500 0.0027409139598838462 0.5683226454301991 >1k 0.0054818279197676924 2.93809864521575 >5k 0.002055685469912885 4.6433019117943095 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC 6989 1.6389636703030757 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG 6584 1.5439886686615325 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC 6158 1.4440890373052426 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2892 0.6781918635736865 No Hit GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC 2274 0.5332670462539983 No Hit GAATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT 1435 0.33651636384102357 No Hit GAACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT 1429 0.33510932677966737 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTT 1171 0.274606733141351 No Hit GAATGATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC 1125 0.2638194490042868 No Hit GAATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT 1090 0.25561173281304234 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTA 1069 0.2506871030982956 No Hit CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT 669 0.15688463234121586 Illumina Single End Adapter 1 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC 643 0.15078747174200569 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC 555 0.13015092817544816 No Hit CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA 548 0.12850938493719924 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.690123537853987E-4 0.0 0.0 0.16110574352528445 0.0 2 4.690123537853987E-4 0.0 0.0 1.0189293385987788 0.0 3 4.690123537853987E-4 0.0 0.0 1.331526072396747 0.0 4 4.690123537853987E-4 0.0 0.0 2.05286707251869 0.0 5 4.690123537853987E-4 0.0 0.0 4.713339649366365 0.0 6 4.690123537853987E-4 0.0 0.0 5.851163619649742 0.0 7 4.690123537853987E-4 0.0 0.0 6.94654197191554 0.0 8 4.690123537853987E-4 0.0 0.0 8.363193786524336 0.0 9 4.690123537853987E-4 0.0 0.0 8.931402253135348 0.0 10 4.690123537853987E-4 0.0 0.0 11.454688716500792 0.0 11 4.690123537853987E-4 0.0 0.0 12.623702008310898 0.0 12 4.690123537853987E-4 0.0 0.0 15.062800754171866 0.0 13 4.690123537853987E-4 0.0 0.0 15.58176292363541 0.0 14 4.690123537853987E-4 0.0 0.0 15.819552187004605 0.0 15 4.690123537853987E-4 0.0 0.0 16.362668492688098 0.0 16 4.690123537853987E-4 0.0 0.0 16.940491712551708 0.0 17 4.690123537853987E-4 0.0 0.0 17.581866106353242 0.0 18 4.690123537853987E-4 0.0 0.0 18.278818464078345 0.0 19 7.03518530678098E-4 0.0 0.0 18.978350389749266 0.0 20 7.03518530678098E-4 0.0 0.0 19.405620644047765 0.0 21 7.03518530678098E-4 0.0 0.0 19.8605626272196 0.0 22 7.03518530678098E-4 0.0 0.0 20.344817882503026 0.0 23 7.03518530678098E-4 0.0 0.0 20.789441593891585 0.0 24 7.03518530678098E-4 0.0 0.0 21.16089937808962 0.0 25 7.03518530678098E-4 0.0 0.0 21.50867203842149 0.0 26 7.03518530678098E-4 0.0 0.0 21.844250377554946 0.0 27 7.03518530678098E-4 0.0 0.0 22.199527235547386 0.0 28 7.03518530678098E-4 0.0 0.0 22.51118594463778 0.0 29 7.03518530678098E-4 0.0 0.0 22.819561567251682 0.0 30 7.03518530678098E-4 0.0 0.0 23.177652499366832 0.0 31 7.03518530678098E-4 0.0 0.0 23.492359788756836 0.0 32 7.03518530678098E-4 0.0 0.0 23.81668183139944 0.0 33 7.03518530678098E-4 0.0 0.0 24.107938503100172 0.0 34 7.03518530678098E-4 0.0 0.0 24.441640792818482 0.0 35 7.03518530678098E-4 0.0 0.0 24.76033468721566 0.0 36 7.03518530678098E-4 0.0 0.0 25.065896235706848 0.0 37 7.03518530678098E-4 0.0 0.0 25.399364019248267 0.0 38 7.03518530678098E-4 0.0 0.0 25.692731246541033 0.0 39 7.03518530678098E-4 0.0 0.0 25.99125760972544 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGCA 20 7.0282904E-4 45.0 1 CCGATCG 50 2.1827873E-11 45.0 40 AACCCCG 25 3.8866645E-5 45.0 36 CTAAGCG 20 7.0282904E-4 45.0 1 AGTATCG 20 7.0282904E-4 45.0 1 GTTACGG 20 7.0282904E-4 45.0 2 TACGAAT 50 2.1827873E-11 45.0 12 CGCAACT 25 3.8866645E-5 45.0 43 CGTCATA 40 6.7975634E-9 45.0 38 ATCGATC 20 7.0282904E-4 45.0 29 CGAACCC 30 2.1622782E-6 44.999996 34 CGTTTTT 1365 0.0 41.703297 1 ACAACGA 60 3.6379788E-12 41.249996 13 ACGGCTG 740 0.0 41.0473 8 GATGAAT 740 0.0 41.0473 20 CTACGAA 55 6.002665E-11 40.909092 11 CGAATAT 55 6.002665E-11 40.909092 14 GCTACGA 55 6.002665E-11 40.909092 10 CCGATGA 705 0.0 40.851063 18 CGATGAA 700 0.0 40.821426 19 >>END_MODULE