##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551610_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	502986
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92684488236253	33.0	31.0	34.0	30.0	34.0
2	32.0454068304088	33.0	31.0	34.0	30.0	34.0
3	31.9819597364539	33.0	31.0	34.0	30.0	34.0
4	35.65169607106361	37.0	35.0	37.0	33.0	37.0
5	35.684017050176344	37.0	35.0	37.0	35.0	37.0
6	35.58071198800762	37.0	35.0	37.0	33.0	37.0
7	35.8629325667116	37.0	35.0	37.0	35.0	37.0
8	35.5795051949756	37.0	35.0	37.0	33.0	37.0
9	37.399420659819555	39.0	37.0	39.0	34.0	39.0
10	37.13172931254548	39.0	37.0	39.0	34.0	39.0
11	37.234324613408724	39.0	37.0	39.0	34.0	39.0
12	37.18564134985864	39.0	37.0	39.0	34.0	39.0
13	37.24358729666432	39.0	37.0	39.0	34.0	39.0
14	38.358300231020344	40.0	38.0	41.0	34.0	41.0
15	38.350556476721025	40.0	38.0	41.0	34.0	41.0
16	38.2046100686699	40.0	37.0	41.0	33.0	41.0
17	38.141526801938824	40.0	37.0	41.0	33.0	41.0
18	37.88860723757719	39.0	37.0	41.0	33.0	41.0
19	37.615814356662014	39.0	37.0	41.0	33.0	41.0
20	37.66844604024764	39.0	35.0	41.0	33.0	41.0
21	37.640568524770075	39.0	35.0	41.0	33.0	41.0
22	37.78938578807362	39.0	35.0	41.0	33.0	41.0
23	37.788218757579735	40.0	35.0	41.0	33.0	41.0
24	37.6384968965339	39.0	35.0	41.0	33.0	41.0
25	37.341554238090126	39.0	35.0	41.0	33.0	41.0
26	37.47473488327707	39.0	35.0	41.0	33.0	41.0
27	37.52676018815633	39.0	35.0	41.0	33.0	41.0
28	37.39037070614292	39.0	35.0	41.0	33.0	41.0
29	37.40223187126481	39.0	35.0	41.0	33.0	41.0
30	37.11002691923831	39.0	35.0	41.0	32.0	41.0
31	36.99890851832854	39.0	35.0	41.0	32.0	41.0
32	36.772663652666274	39.0	35.0	41.0	31.0	41.0
33	36.481224129498635	39.0	35.0	41.0	30.0	41.0
34	36.21013904959582	39.0	35.0	41.0	29.0	41.0
35	36.02846003666106	39.0	35.0	41.0	28.0	41.0
36	35.85584091803748	39.0	35.0	41.0	27.0	41.0
37	35.783483039289365	39.0	35.0	41.0	26.0	41.0
38	35.6669052418954	39.0	35.0	41.0	25.0	41.0
39	35.51196653584792	39.0	35.0	41.0	25.0	41.0
40	35.42934594601043	39.0	35.0	41.0	25.0	41.0
41	35.26287809203437	38.0	35.0	40.0	24.0	41.0
42	35.22684726811482	39.0	35.0	41.0	24.0	41.0
43	35.2592159622733	39.0	35.0	41.0	23.0	41.0
44	35.326931564695634	39.0	35.0	41.0	24.0	41.0
45	35.32865725885015	39.0	35.0	41.0	24.0	41.0
46	35.220085648507116	38.0	35.0	40.0	23.0	41.0
47	35.10158930864875	38.0	35.0	40.0	23.0	41.0
48	35.07063417272051	38.0	35.0	40.0	23.0	41.0
49	35.07600609162084	38.0	35.0	40.0	24.0	41.0
50	34.95666877408119	38.0	35.0	40.0	24.0	41.0
51	34.070180879785916	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	11.0
10	21.0
11	22.0
12	10.0
13	16.0
14	20.0
15	29.0
16	66.0
17	92.0
18	174.0
19	366.0
20	728.0
21	1225.0
22	2085.0
23	2942.0
24	4058.0
25	5969.0
26	8464.0
27	9230.0
28	8744.0
29	7917.0
30	8455.0
31	9474.0
32	12148.0
33	16224.0
34	27386.0
35	36139.0
36	40893.0
37	58293.0
38	100957.0
39	140778.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.760208037599455	17.733893189870095	20.485262015244956	20.02063675728549
2	33.371704182621386	29.23739428135177	21.921087266842417	15.46981426918443
3	27.78367588759926	27.966185937580768	29.976182239664723	14.273955935155252
4	25.14225048013265	22.11453201480757	36.43481130687534	16.308406198184443
5	27.217854970118456	25.942272747153993	28.787282349806954	18.052589932920597
6	22.99586867228909	34.68565725487389	28.79265029245347	13.52582378038355
7	68.35816503839072	5.782268293749727	21.175738489739278	4.683828178120266
8	67.68756983295758	10.034871745933287	16.495091314668798	5.782467106440338
9	61.72179742577328	7.314517700293845	18.38043205973924	12.583252814193635
10	36.9572910578028	21.78032788188936	25.184995208614158	16.077385851693684
11	29.01870032167893	21.217091529386504	30.727893022867438	19.036315126067127
12	23.330669243279136	18.8518169491795	35.123840424982006	22.693673382559357
13	22.925091354431338	20.389434298370134	38.39351393478149	18.291960412417048
14	19.655020219250634	26.076868938698095	33.52001844981769	20.74809239223358
15	18.295936666229277	21.940968535903586	39.38817382591166	20.374920971955483
16	19.519032338872254	22.644169022597048	33.321802197277854	24.514996441252837
17	19.67251573602446	24.686571793250707	33.45122925886605	22.189683211858778
18	19.838524332685203	23.360689959561498	33.62300342355453	23.177782284198763
19	20.908335420866585	24.35813322836023	30.91835558047341	23.81517577029977
20	22.259665278954085	24.707645938455546	33.35957660849407	19.673112174096296
21	21.22524284970158	28.084081863113486	31.412007491262184	19.27866779592275
22	19.84409108802233	22.787115347146838	32.50746541653247	24.861328148298362
23	21.505568743464032	25.589579034008896	31.299479508375978	21.605372714151088
24	21.433996174843834	24.299682297320402	31.237251136214528	23.029070391621236
25	20.093799827430583	28.856667978830426	28.96681020942929	22.082721984309703
26	19.54467917596116	24.617384976917847	32.60249788264484	23.235437964476148
27	24.7802125705288	24.07065007773576	29.395052745006815	21.754084606728615
28	19.906518272874393	25.07564822877774	32.39692556055238	22.620907937795483
29	22.261852218550814	24.553764915922113	30.41814285089446	22.766240014632615
30	23.205019622812564	26.700146723765673	29.089080014155467	21.005753639266302
31	22.906402961513837	23.534253438465484	26.864167193520295	26.69517640650038
32	23.272218312239307	26.447654606688854	28.252476212061566	22.02765086901027
33	22.360860938475426	26.290791393796248	26.295761711061537	25.052585956666785
34	20.39639274254154	26.352423327885866	26.946873272814752	26.30431065675784
35	22.999049675338874	24.590744076375884	26.485627830595682	25.92457841768956
36	20.71568592366388	30.381958941203134	24.84204331730903	24.06031181782396
37	21.521672571403577	25.463531788161102	29.519708302020337	23.495087338414987
38	19.86675573475206	27.563192613710918	25.949231191325406	26.620820460211615
39	21.538372837414958	26.40669919242285	28.289654185205947	23.765273784956243
40	20.74212801151523	25.973883964961253	28.761834325408657	24.522153698114856
41	19.535931417574247	27.29618717021945	26.276079254690988	26.891802157515322
42	20.680496077425616	25.19493584314474	29.381135856664002	24.743432222765644
43	22.582537088507433	25.974480403033084	27.93676165937024	23.50622084908924
44	22.230241000743558	24.195902072821113	28.79424079397836	24.779616132456965
45	21.34671740366531	22.903023145773442	28.556659628697417	27.193599821863828
46	24.1752255529975	25.8691096770089	27.31467675044634	22.640988019547265
47	20.320247482037274	24.049377119840315	32.46651000226646	23.163865395855947
48	21.124444815561468	24.63328999216678	28.402778606163988	25.839486586107764
49	20.893026843689487	22.742780117140438	32.19413661612848	24.170056423041594
50	21.20933783445265	22.676973116547973	30.869447658582942	25.244241390416434
51	20.404146437475397	22.559872441777703	28.75606875738092	28.279912363365977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	192.0
1	413.0
2	634.0
3	7192.5
4	13751.0
5	8938.5
6	4126.0
7	3979.5
8	3833.0
9	3833.0
10	3833.0
11	3802.0
12	3771.0
13	3722.0
14	3673.0
15	3588.0
16	3503.0
17	3243.0
18	2983.0
19	2820.0
20	2657.0
21	2558.0
22	2459.0
23	2474.0
24	2489.0
25	2761.0
26	3241.5
27	3450.0
28	4113.5
29	4777.0
30	5511.5
31	6246.0
32	6847.5
33	7449.0
34	8280.0
35	9111.0
36	9902.0
37	10693.0
38	11353.5
39	12014.0
40	14129.5
41	16245.0
42	18035.5
43	19826.0
44	23257.0
45	26688.0
46	45147.5
47	63607.0
48	55690.0
49	47773.0
50	46315.5
51	44858.0
52	38945.0
53	33032.0
54	29429.0
55	25826.0
56	24153.0
57	22480.0
58	20691.5
59	18903.0
60	18403.0
61	17903.0
62	16603.5
63	15304.0
64	13593.0
65	11882.0
66	10658.0
67	9434.0
68	8286.0
69	7138.0
70	6518.5
71	5899.0
72	5036.5
73	4174.0
74	3678.5
75	2553.0
76	1923.0
77	1490.0
78	1057.0
79	854.5
80	652.0
81	485.5
82	319.0
83	220.0
84	121.0
85	76.5
86	32.0
87	36.0
88	40.0
89	24.5
90	9.0
91	4.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	502986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.628333988390345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.05614543309947	29.74349999104065
2	8.10060818917315	6.420272136592871
3	3.5599485020057253	4.232244846568585
4	2.2851738205701184	3.622305255323146
5	1.6987083587153093	3.365849109402034
6	1.3640695589687613	3.243348243972624
7	1.1778194732828933	3.267251642569915
8	1.0588260566874368	3.3567610088016058
9	0.8900048541078599	3.1742468648887403
>10	4.726638742954178	27.18233568910504
>50	0.045810969015945134	1.2544300053356716
>100	0.02768794830631845	2.215401591800928
>500	0.003523920693531439	0.9847115945186298
>1k	0.0030205034515983762	1.9638373047895847
>5k	0.002013668967732251	5.973504715289978
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9480	1.8847443069986043	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC	8199	1.6300652503250586	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC	6204	1.2334339325547827	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG	6060	1.2048049051067027	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	2790	0.5546874068065513	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT	2316	0.460450191456621	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCT	1446	0.28748315062447066	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTACCAT	1183	0.2351954129936022	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTC	1102	0.21909158505405718	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTA	1007	0.2002043794459488	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTT	913	0.18151598652845208	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC	848	0.16859316163869373	No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	819	0.1628275936109554	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTT	664	0.13201162656614696	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	586	0.11650423669843693	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG	571	0.11352204633926194	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	535	0.10636478947724191	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16720147280441205	0.0
2	0.0	0.0	0.0	0.9825323170028589	0.0
3	0.0	0.0	0.0	1.3487452931095498	0.0
4	0.0	0.0	0.0	2.227099760231895	0.0
5	0.0	0.0	0.0	4.903913826627381	0.0
6	0.0	0.0	0.0	6.241525609062678	0.0
7	0.0	0.0	0.0	7.525060339651601	0.0
8	0.0	0.0	0.0	9.318350808968837	0.0
9	0.0	0.0	0.0	10.187758705013659	0.0
10	0.0	0.0	0.0	12.665958893488089	0.0
11	0.0	0.0	0.0	13.978520276906316	0.0
12	0.0	0.0	0.0	16.332661346439068	0.0
13	0.0	0.0	0.0	16.936057862445477	0.0
14	0.0	0.0	0.0	17.22751726688218	0.0
15	0.0	0.0	0.0	17.73289912641704	0.0
16	0.0	0.0	0.0	18.282218590577074	0.0
17	0.0	0.0	0.0	18.921401390893582	0.0
18	0.0	0.0	0.0	19.656014282703694	0.0
19	0.0	0.0	0.0	20.328995240424188	0.0
20	0.0	0.0	0.0	20.78149292425634	0.0
21	0.0	0.0	0.0	21.235382296922776	0.0
22	0.0	0.0	0.0	21.692651485329613	0.0
23	0.0	0.0	0.0	22.141371728040145	0.0
24	0.0	0.0	0.0	22.536611356976138	0.0
25	0.0	0.0	0.0	22.877773934065758	0.0
26	0.0	0.0	0.0	23.21038756545908	0.0
27	0.0	0.0	0.0	23.56606346896335	0.0
28	0.0	0.0	0.0	23.902852166859514	0.0
29	0.0	0.0	0.0	24.239044426683844	0.0
30	0.0	0.0	0.0	24.649791445487548	0.0
31	0.0	0.0	0.0	25.013221043925675	0.0
32	0.0	0.0	0.0	25.354980059087133	0.0
33	0.0	0.0	0.0	25.660555164557262	0.0
34	0.0	0.0	0.0	25.981240034513885	0.0
35	1.9881269061166712E-4	0.0	0.0	26.304310656757842	0.0
36	1.9881269061166712E-4	0.0	0.0	26.61763945716183	0.0
37	1.9881269061166712E-4	0.0	0.0	26.937727889046613	0.0
38	1.9881269061166712E-4	0.0	0.0	27.242110118373077	0.0
39	1.9881269061166712E-4	0.0	0.0	27.576711876672512	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	30	2.1630567E-6	45.000004	25
GCGATAT	30	2.1630567E-6	45.000004	9
GTACGAG	30	2.1630567E-6	45.000004	1
CAAACGC	20	7.0295E-4	45.0	25
CAAACGA	45	3.8380676E-10	45.0	15
ACCGAAT	20	7.0295E-4	45.0	39
GCAAACG	20	7.0295E-4	45.0	1
ACGATGA	45	3.8380676E-10	45.0	18
CCGGTAT	25	3.887667E-5	45.0	42
ACCGTCC	25	3.887667E-5	45.0	27
GTAAGCG	20	7.0295E-4	45.0	1
ATACCGG	20	7.0295E-4	45.0	2
CGTTTTT	4390	0.0	43.052395	1
CGATGAA	660	0.0	41.590908	19
GATGAAT	695	0.0	41.43885	20
CGAATAT	60	3.6379788E-12	41.250004	14
GCTACGA	60	3.6379788E-12	41.250004	10
CATTCTA	55	6.002665E-11	40.909092	45
CCGATGA	630	0.0	40.0	18
CCTATGC	45	1.9246727E-8	40.0	35
>>END_MODULE