SRR3551609_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551609_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	271578
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	8800	3.2403213809660576	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	8095	2.9807274521500267	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	7998	2.945010273291651	No Hit
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	3086	1.1363217933705971	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2631	0.9687824492411019	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	1907	0.7021923719888945	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTA	1554	0.5722112984115061	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTATCTGT	1521	0.5600600932328834	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	1504	0.553800381474199	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTC	1347	0.4959901022910545	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	669	0.24633806862116964	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	512	0.18852778943802517	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	488	0.17969054930811773	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	487	0.1793223309693716	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATG	455	0.16753934412949503	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	435	0.16017497735457215	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	434	0.15980675901582603	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTAT	386	0.14213227875601114	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTATCTG	335	0.12335314347995786	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGT	321	0.11819808673751188	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	310	0.11414768501130432	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCTGTCGAATGC	301	0.110833719962589	No Hit
ACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	291	0.10715153657512759	TruSeq Adapter, Index 19 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	280	0.10310113484892003	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	25	3.8829123E-5	45.000004	13
CTAGATG	20	7.0237607E-4	45.000004	32
CTGTCGA	45	3.8198777E-10	45.000004	41
TGCCCCG	25	3.8829123E-5	45.000004	18
ATTACGG	20	7.0237607E-4	45.000004	2
CAACCCT	20	7.0237607E-4	45.000004	29
AAGGTCA	20	7.0237607E-4	45.000004	5
TCGATTA	20	7.0237607E-4	45.000004	41
TGCATAG	20	7.0237607E-4	45.000004	1
CTAGTCT	25	3.8829123E-5	45.000004	28
CGCTATT	25	3.8829123E-5	45.000004	45
CTACGAA	60	0.0	45.000004	11
TATTAGT	45	3.8198777E-10	45.000004	30
AGATTAG	20	7.0237607E-4	45.000004	10
CGAATAT	60	0.0	45.000004	14
CTACCTT	20	7.0237607E-4	45.000004	17
CGTCGAA	60	0.0	45.000004	42
TTGTTAG	30	2.1593587E-6	45.000004	1
CGATCAC	20	7.0237607E-4	45.000004	34
CCTGCGT	20	7.0237607E-4	45.000004	23

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content