##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551609_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	271578
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80593420674723	33.0	31.0	34.0	30.0	34.0
2	31.885579833418024	33.0	31.0	34.0	30.0	34.0
3	31.883639322772833	33.0	31.0	34.0	30.0	34.0
4	35.60537672418237	37.0	35.0	37.0	33.0	37.0
5	35.59678619033942	37.0	35.0	37.0	33.0	37.0
6	35.48321292593656	37.0	35.0	37.0	33.0	37.0
7	35.75360669862802	37.0	35.0	37.0	35.0	37.0
8	35.32031681505866	37.0	35.0	37.0	33.0	37.0
9	37.08133574884564	39.0	37.0	39.0	34.0	39.0
10	36.959238229900805	39.0	37.0	39.0	33.0	39.0
11	37.080223729462624	39.0	37.0	39.0	34.0	39.0
12	36.94392402919235	39.0	37.0	39.0	33.0	39.0
13	36.84323104227883	39.0	35.0	39.0	33.0	39.0
14	37.8414488655193	40.0	37.0	41.0	33.0	41.0
15	37.80626560325211	40.0	36.0	41.0	33.0	41.0
16	37.80884681380672	40.0	36.0	41.0	33.0	41.0
17	37.6421654184065	39.0	36.0	41.0	32.0	41.0
18	37.526246603185825	39.0	36.0	41.0	33.0	41.0
19	37.34685431073209	39.0	36.0	40.0	32.0	41.0
20	37.31004352340764	39.0	35.0	41.0	32.0	41.0
21	37.39520137860946	39.0	35.0	41.0	33.0	41.0
22	37.51342524063068	39.0	35.0	41.0	33.0	41.0
23	37.45918299714999	39.0	35.0	41.0	33.0	41.0
24	37.34401902952374	39.0	35.0	41.0	32.0	41.0
25	37.120554684105485	39.0	35.0	41.0	32.0	41.0
26	37.27191819661386	39.0	35.0	41.0	33.0	41.0
27	37.223744191355706	39.0	35.0	41.0	32.0	41.0
28	37.063480841599834	39.0	35.0	41.0	32.0	41.0
29	37.11598877670504	39.0	35.0	41.0	32.0	41.0
30	36.88597382704048	39.0	35.0	41.0	31.0	41.0
31	36.867621825037375	39.0	35.0	41.0	31.0	41.0
32	36.609769568963614	39.0	35.0	41.0	31.0	41.0
33	36.509363792354314	39.0	35.0	41.0	30.0	41.0
34	36.37264432317787	39.0	35.0	41.0	30.0	41.0
35	36.22013933381938	39.0	35.0	41.0	30.0	41.0
36	36.08238148892767	39.0	35.0	41.0	30.0	41.0
37	36.0359749316955	39.0	35.0	41.0	29.0	41.0
38	35.89864790226012	39.0	35.0	41.0	29.0	41.0
39	35.83413236712841	39.0	35.0	41.0	29.0	41.0
40	35.76069490164888	39.0	35.0	40.0	28.0	41.0
41	35.650884828668005	38.0	35.0	40.0	27.0	41.0
42	35.57146749736724	39.0	35.0	40.0	27.0	41.0
43	35.52338554669377	38.0	35.0	40.0	28.0	41.0
44	35.67312521632827	38.0	35.0	40.0	28.0	41.0
45	35.67562541884836	38.0	35.0	40.0	28.0	41.0
46	35.58596425336368	38.0	35.0	40.0	28.0	41.0
47	35.34578647754973	38.0	35.0	40.0	27.0	41.0
48	35.38583390407176	38.0	35.0	40.0	27.0	41.0
49	35.44007614755245	38.0	35.0	40.0	28.0	41.0
50	35.21335306983629	38.0	35.0	40.0	27.0	41.0
51	34.42933890079461	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	8.0
11	2.0
12	6.0
13	13.0
14	8.0
15	12.0
16	16.0
17	44.0
18	93.0
19	245.0
20	531.0
21	1087.0
22	1603.0
23	1987.0
24	2381.0
25	2772.0
26	3265.0
27	3489.0
28	3551.0
29	3728.0
30	4433.0
31	5561.0
32	7436.0
33	10029.0
34	17461.0
35	23837.0
36	22083.0
37	31519.0
38	54068.0
39	70273.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.57994388352518	14.736097916620638	20.012298492514123	14.671659707340066
2	26.37400673103123	37.141079174307194	21.53340844987444	14.951505644787133
3	26.024199309222396	35.28673162038162	25.595961381260636	13.09310768913535
4	23.76149761762735	20.671409318869717	40.100818181148696	15.466274882354242
5	33.13928226881412	23.84545139886147	23.75486968752992	19.260396644794497
6	21.83387461429129	39.4081994859672	25.57718224598458	13.180743653756933
7	64.02212255779187	5.681608966852985	24.66731473094286	5.628953744412287
8	61.14486445882951	16.774186421580538	14.457356634189807	7.623592485400144
9	56.818667196901075	6.951962235527179	15.381584664442627	20.84778590312912
10	40.75109176737438	19.267392793230677	22.407927004396527	17.573588434998417
11	33.147014853927786	19.240144636163457	26.837225401173882	20.775615108734875
12	25.095184440565877	17.084962699482283	31.89654537554588	25.923307484405957
13	24.62312853029332	21.162980801095816	37.912864812319114	16.301025856291744
14	19.47948655634845	30.370648579781868	27.606433510814572	22.54343135305511
15	15.095479015236876	20.713017991148032	42.221019375648986	21.970483617966106
16	16.62616264940459	22.198042551311225	30.49657925163305	30.679215547651133
17	16.65193793311682	24.632333988761975	33.07484405953354	25.640884018587663
18	17.647232102747644	22.634381282725403	32.79610277710271	26.92228383742424
19	19.350610137787303	24.164696698554376	28.569324466635738	27.915368697022586
20	22.43554337980249	24.784776381002878	33.419496424599934	19.360183814594702
21	19.653285612236633	32.03131328752698	28.54502205627849	19.770379043957906
22	17.713511403721952	21.542245690004343	31.703598966042907	29.040643940230797
23	20.087415033618335	28.546863147972225	28.844383565679106	22.521338252730338
24	21.977847984741032	23.960335520550267	27.22937793193852	26.83243856277018
25	17.60010015538814	33.02439814712532	26.336816678817872	23.03868501866867
26	18.99932984262348	22.609342435690667	31.590924154386585	26.800403567299263
27	24.914389236241522	24.89045504422302	25.226270169159505	24.96888555037595
28	16.84304325092607	24.931327279823844	34.37465479530742	23.85097467394266
29	24.64632628563433	21.03962765761586	28.233877560038	26.08016849671181
30	19.35760628622348	26.38063466112866	29.81463888827519	24.447120164372667
31	23.45882214317802	21.282651761188315	25.966757248378	29.291768847255668
32	25.785593825714898	28.839596727275406	24.553167045931556	20.821642401078144
33	22.92306446030238	20.169527723158726	27.043427670871722	29.863980145667174
34	24.50014360515211	21.583854362282658	26.28047927298971	27.63552275957552
35	21.566179882022844	20.468152795881846	28.89446125974858	29.07120606234673
36	27.94372150910604	22.190309966197557	27.977597596270687	21.88837092842572
37	18.919058244776824	24.39998821701316	30.446501557563572	26.234451980646444
38	19.517413045239305	23.368608650185212	31.602338922887714	25.51163938168777
39	23.5847528150292	25.24394464941932	26.512456826399784	24.6588457091517
40	19.713673419791	20.02665900772522	35.781617067656434	24.478050504827344
41	22.434806943124997	21.647924353224486	25.54956587057862	30.367702833071895
42	21.91598730383168	19.696367157869933	35.16337847690166	23.224267061396723
43	28.43197902628343	19.675746930900146	26.87257436169351	25.01969968112292
44	22.13470899704689	19.850650641804567	31.125127955872717	26.889512405275834
45	20.06679480664855	19.05861299516161	30.291481636951445	30.583110561238392
46	28.76926702457489	23.327368196245647	26.886566658565865	21.0167981206136
47	18.289773103859662	20.985867780158923	37.96073319635611	22.7636259196253
48	22.146491983886765	22.196937896294987	27.72905021761704	27.927519902201208
49	20.960828933124183	17.6468638844089	37.0541796463631	24.33812753610381
50	23.37081796021769	18.476091583265212	31.251058627723893	26.9020318287932
51	21.804048928852854	18.722429651886383	27.309281311446437	32.16424010781433
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	139.0
1	266.0
2	393.0
3	2083.5
4	3774.0
5	2490.5
6	1207.0
7	1173.0
8	1139.0
9	1135.0
10	1131.0
11	1148.5
12	1166.0
13	1109.0
14	1052.0
15	1021.5
16	991.0
17	892.5
18	794.0
19	804.5
20	815.0
21	828.0
22	841.0
23	880.5
24	920.0
25	1055.5
26	1292.0
27	1393.0
28	1803.5
29	2214.0
30	2400.5
31	2587.0
32	2847.5
33	3108.0
34	3425.0
35	3742.0
36	4015.5
37	4289.0
38	4687.5
39	5086.0
40	6343.0
41	7600.0
42	9372.5
43	11145.0
44	13346.0
45	15547.0
46	34140.0
47	52733.0
48	40989.5
49	29246.0
50	28319.0
51	27392.0
52	22302.5
53	17213.0
54	15356.0
55	13499.0
56	12464.0
57	11429.0
58	10530.0
59	9631.0
60	9014.5
61	8398.0
62	7682.5
63	6967.0
64	6356.0
65	5745.0
66	5187.5
67	4630.0
68	4034.5
69	3439.0
70	3127.5
71	2816.0
72	2569.0
73	2322.0
74	1961.5
75	1277.5
76	954.0
77	819.0
78	684.0
79	508.5
80	333.0
81	258.0
82	183.0
83	125.0
84	67.0
85	43.0
86	19.0
87	15.0
88	11.0
89	6.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	271578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.92285089366591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85289871590305	25.73146572991921
2	9.218795792764336	6.254556701941984
3	3.273741682677174	3.3316395289751015
4	1.8018604752135639	2.444969769274389
5	1.1701238507712182	1.9846968458417102
6	0.888990198313198	1.809424916598546
7	0.7717607216125566	1.8326226719395533
8	0.63607845691274	1.726207572041918
9	0.6154547526783679	1.8790181826215673
>10	5.638954920924376	33.525911524497566
>50	0.0738111519967002	1.6857035547798425
>100	0.04450378282153983	3.2034995470914436
>500	0.0021709162351970647	0.43486585805919475
>1k	0.007598206823189727	4.989358490010236
>5k	0.0032563743527955972	9.166059106407735
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	8800	3.2403213809660576	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	8095	2.9807274521500267	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	7998	2.945010273291651	No Hit
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	3086	1.1363217933705971	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2631	0.9687824492411019	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	1907	0.7021923719888945	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTA	1554	0.5722112984115061	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTATCTGT	1521	0.5600600932328834	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	1504	0.553800381474199	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTC	1347	0.4959901022910545	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	669	0.24633806862116964	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	512	0.18852778943802517	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	488	0.17969054930811773	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	487	0.1793223309693716	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATG	455	0.16753934412949503	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	435	0.16017497735457215	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	434	0.15980675901582603	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTAT	386	0.14213227875601114	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTATCTG	335	0.12335314347995786	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGT	321	0.11819808673751188	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	310	0.11414768501130432	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCTGTCGAATGC	301	0.110833719962589	No Hit
ACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	291	0.10715153657512759	TruSeq Adapter, Index 19 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	280	0.10310113484892003	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.364366774922858E-4	0.0	0.0	0.2540706537348386	0.0
2	7.364366774922858E-4	0.0	0.0	1.9106849597537356	0.0
3	7.364366774922858E-4	0.0	0.0	2.325667027520639	0.0
4	7.364366774922858E-4	0.0	0.0	3.4616206025524896	0.0
5	7.364366774922858E-4	0.0	0.0	7.859252222197674	0.0
6	7.364366774922858E-4	0.0	0.0	8.982686373712156	0.0
7	7.364366774922858E-4	0.0	0.0	10.708525727415328	0.0
8	7.364366774922858E-4	0.0	0.0	12.787854686314796	0.0
9	7.364366774922858E-4	0.0	0.0	13.559640324326713	0.0
10	7.364366774922858E-4	0.0	0.0	17.94548895713202	0.0
11	7.364366774922858E-4	0.0	0.0	19.526618503707958	0.0
12	7.364366774922858E-4	0.0	0.0	24.225820942786235	0.0
13	7.364366774922858E-4	0.0	0.0	24.98692824897451	0.0
14	7.364366774922858E-4	0.0	0.0	25.35183262267194	0.0
15	7.364366774922858E-4	0.0	0.0	26.319510416896804	0.0
16	7.364366774922858E-4	0.0	0.0	27.17709092783657	0.0
17	7.364366774922858E-4	0.0	0.0	28.15102843382012	0.0
18	7.364366774922858E-4	0.0	0.0	29.197873170875404	0.0
19	7.364366774922858E-4	0.0	0.0	30.37322610815309	0.0
20	7.364366774922858E-4	0.0	0.0	31.03601911789615	0.0
21	0.0011046550162384287	0.0	0.0	31.64578868685976	0.0
22	0.0011046550162384287	0.0	0.0	32.26660480598576	0.0
23	0.0011046550162384287	0.0	0.0	32.903622532016584	0.0
24	0.0011046550162384287	0.0	0.0	33.36426367378801	0.0
25	0.0011046550162384287	0.0	0.0	33.75862551458513	0.0
26	0.0011046550162384287	0.0	0.0	34.139731495187384	0.0
27	0.0011046550162384287	0.0	0.0	34.61804711721862	0.0
28	0.0011046550162384287	0.0	0.0	34.995470914433426	0.0
29	0.0011046550162384287	0.0	0.0	35.34969695630721	0.0
30	0.0011046550162384287	0.0	0.0	35.79155896280258	0.0
31	0.0011046550162384287	0.0	0.0	36.16971919669487	0.0
32	0.0011046550162384287	0.0	0.0	36.55487557902334	0.0
33	0.0011046550162384287	0.0	0.0	36.940031961351806	0.0
34	0.0011046550162384287	0.0	0.0	37.29278512987061	0.0
35	0.0011046550162384287	0.0	0.0	37.69929817584635	0.0
36	0.0014728733549845717	0.0	0.0	38.04100479420277	0.0
37	0.0014728733549845717	0.0	0.0	38.390812216011604	0.0
38	0.0014728733549845717	0.0	0.0	38.720735847528154	0.0
39	0.0014728733549845717	0.0	0.0	39.06870217764325	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	25	3.8829123E-5	45.000004	13
CTAGATG	20	7.0237607E-4	45.000004	32
CTGTCGA	45	3.8198777E-10	45.000004	41
TGCCCCG	25	3.8829123E-5	45.000004	18
ATTACGG	20	7.0237607E-4	45.000004	2
CAACCCT	20	7.0237607E-4	45.000004	29
AAGGTCA	20	7.0237607E-4	45.000004	5
TCGATTA	20	7.0237607E-4	45.000004	41
TGCATAG	20	7.0237607E-4	45.000004	1
CTAGTCT	25	3.8829123E-5	45.000004	28
CGCTATT	25	3.8829123E-5	45.000004	45
CTACGAA	60	0.0	45.000004	11
TATTAGT	45	3.8198777E-10	45.000004	30
AGATTAG	20	7.0237607E-4	45.000004	10
CGAATAT	60	0.0	45.000004	14
CTACCTT	20	7.0237607E-4	45.000004	17
CGTCGAA	60	0.0	45.000004	42
TTGTTAG	30	2.1593587E-6	45.000004	1
CGATCAC	20	7.0237607E-4	45.000004	34
CCTGCGT	20	7.0237607E-4	45.000004	23
>>END_MODULE