FastQCFastQC Report
Sat 18 Jun 2016
SRR3551608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719002
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132651.8449183729669736No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC28900.401946030748176No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG18780.26119537915054475No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC17760.2470090486535503No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC11540.16050024895619205No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG11010.1531289203646165No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCC9120.12684248444371504No Hit
GAACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT8240.11460329734826885No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA7890.10973543884439821No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCCG207.0315227E-445.023
GTAATCG207.0315227E-445.01
CGTTTTT58950.043.0152661
TACGAAT800.042.187512
GTTGATC1200.041.24999616
CGGTCTA451.9264917E-840.031
CGAATAT850.039.70588314
TGCGTAG800.039.3751
TAGGGCG2900.038.7931025
TTAAGCG356.245027E-638.5714261
GCGATCA1000.038.2499969
CTACGAA950.037.89473711
TCGATAG301.13949696E-437.4999961
TATAGCG301.13949696E-437.4999961
GACGAAT301.13949696E-437.49999620
CGATAAC301.13949696E-437.49999616
TAATCGG301.13949696E-437.4999962
GTTTTTT70100.037.328812
GCGTTTA250.002106585535.99999632
ACTACGG504.8745278E-835.9999962