FastQCFastQC Report
Sat 18 Jun 2016
SRR3551606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747286
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129961.7390931985879572No Hit
GCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC12120.16218690032999414TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCC11160.14934041317514313No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG8470.11334348562665432No Hit
CTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGCT8400.11240676260494643TruSeq Adapter, Index 14 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTT8250.11039949898700097No Hit
CCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC8210.10986422868888217TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTG7560.10116608634445179TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG253.889492E-545.01
GACGCAT207.0317E-445.09
CGTTTTT58750.043.3148961
CGTAAGG451.9266736E-840.02
ACATACG403.4560617E-739.37517
TATAGCG356.2453546E-638.571431
AACACGT700.038.5714341
TCGCAAC301.13954E-437.49999616
ACCGGGC601.546141E-1037.4999964
TATTGCG301.13954E-437.4999961
CACTAGG601.546141E-1037.4999962
CGGGCTA301.13954E-437.4999966
GCGTAAG301.13954E-437.4999961
GTTTTTT71600.036.8610342
TCGGCGT1600.036.56254
TTAGGCG504.8750735E-836.01
ACCGGTT250.002106638236.039
GACCGGT504.8750735E-836.03
TCTACGG250.002106638236.02
ACTAGGG2700.035.8333323
Produced by FastQC (version 0.11.5)