##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551606_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	747286
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11151821391007	33.0	31.0	34.0	30.0	34.0
2	32.23447916861817	33.0	31.0	34.0	30.0	34.0
3	32.15284509545208	33.0	31.0	34.0	30.0	34.0
4	35.80680221494849	37.0	35.0	37.0	35.0	37.0
5	35.851161402729346	37.0	35.0	37.0	35.0	37.0
6	35.75697524107236	37.0	35.0	37.0	35.0	37.0
7	36.047376774086494	37.0	35.0	37.0	35.0	37.0
8	35.90020955832171	37.0	35.0	37.0	35.0	37.0
9	37.75390546591265	39.0	38.0	39.0	35.0	39.0
10	37.41445176277891	39.0	37.0	39.0	34.0	39.0
11	37.44006846107113	39.0	37.0	39.0	35.0	39.0
12	37.38944125809931	39.0	37.0	39.0	35.0	39.0
13	37.49724603431618	39.0	37.0	39.0	35.0	39.0
14	38.60703131063609	40.0	38.0	41.0	35.0	41.0
15	38.68640520496838	40.0	38.0	41.0	35.0	41.0
16	38.45117665793284	40.0	38.0	41.0	34.0	41.0
17	38.47080903429209	40.0	38.0	41.0	34.0	41.0
18	38.189638237569014	40.0	38.0	41.0	34.0	41.0
19	37.93841581402569	39.0	37.0	41.0	34.0	41.0
20	38.12883019352697	40.0	36.0	41.0	34.0	41.0
21	37.9852318924749	40.0	36.0	41.0	34.0	41.0
22	38.14457650752188	40.0	36.0	41.0	34.0	41.0
23	38.18069387088745	40.0	36.0	41.0	34.0	41.0
24	38.05272144801321	40.0	36.0	41.0	34.0	41.0
25	37.68362982847263	39.0	35.0	41.0	33.0	41.0
26	37.86473318113814	40.0	35.0	41.0	34.0	41.0
27	37.965206092446536	40.0	36.0	41.0	34.0	41.0
28	37.85802222977548	40.0	36.0	41.0	34.0	41.0
29	37.84274160093994	40.0	36.0	41.0	34.0	41.0
30	37.52136665212516	40.0	35.0	41.0	33.0	41.0
31	37.33859057977802	40.0	35.0	41.0	33.0	41.0
32	37.26135509028672	40.0	35.0	41.0	33.0	41.0
33	37.060251362932	40.0	35.0	41.0	32.0	41.0
34	36.81951622270456	40.0	35.0	41.0	31.0	41.0
35	36.705606688737646	40.0	35.0	41.0	31.0	41.0
36	36.51966315440139	40.0	35.0	41.0	30.0	41.0
37	36.54152359337657	40.0	35.0	41.0	31.0	41.0
38	36.373047267043674	39.0	35.0	41.0	30.0	41.0
39	36.30602741119197	39.0	35.0	41.0	30.0	41.0
40	36.217900509309686	39.0	35.0	41.0	30.0	41.0
41	36.040457067307564	39.0	35.0	41.0	29.0	41.0
42	36.092860029493394	39.0	35.0	41.0	29.0	41.0
43	36.06729284370375	39.0	35.0	41.0	29.0	41.0
44	36.06600953316401	39.0	35.0	41.0	29.0	41.0
45	36.03060675564643	39.0	35.0	41.0	29.0	41.0
46	35.941703711831885	39.0	35.0	41.0	29.0	41.0
47	35.90545520724328	39.0	35.0	41.0	29.0	41.0
48	35.84088421300546	39.0	35.0	41.0	29.0	41.0
49	35.80499969221958	39.0	35.0	41.0	29.0	41.0
50	35.7059479235527	39.0	35.0	41.0	28.0	41.0
51	34.89535867124501	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	17.0
9	22.0
10	34.0
11	27.0
12	25.0
13	31.0
14	47.0
15	61.0
16	86.0
17	141.0
18	219.0
19	412.0
20	669.0
21	996.0
22	1626.0
23	2327.0
24	3882.0
25	6580.0
26	10029.0
27	11603.0
28	11330.0
29	10535.0
30	10646.0
31	12066.0
32	14758.0
33	21039.0
34	39263.0
35	50556.0
36	55347.0
37	82278.0
38	155808.0
39	244722.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.08734808359851	20.026201481092915	20.72446158498888	18.16198885031969
2	37.99950755132573	22.92776794962036	22.609683574963267	16.463040924090645
3	30.68891963719379	22.689305031808438	30.63619551282909	15.985579818168679
4	26.955007855091623	24.82396298070618	30.18375829334418	18.03727087085801
5	24.364701064920258	28.129257071589723	28.857224677031283	18.648817186458732
6	25.971850135021935	31.781272498079716	27.91822675655639	14.328650610341958
7	75.5864555203764	5.655398334774103	14.166731345161024	4.591414799688473
8	76.49159759449527	5.0073198213267744	13.862296363105958	4.638786221071986
9	69.84809028939388	7.515730255885966	16.186038544814167	6.45014090990598
10	35.4692848521182	28.308706439034054	21.98221296799351	14.23979574085424
11	27.18798425234783	23.190853301145747	31.079399319671452	18.541763126834972
12	24.566899420034634	21.20727539389203	34.476358449107835	19.7494667369655
13	24.055983920480244	21.036657986366667	35.723672061299155	19.183686031853934
14	20.850517740195855	24.68238398685376	34.13432072860993	20.332777544340452
15	19.50899655553563	23.62335170202573	37.21025149674957	19.657400245689065
16	22.557227085747623	22.8171008154843	35.089644393177444	19.53602770559063
17	22.4120350173829	22.63631327229468	32.81795724796129	22.13369446236113
18	22.735204459872126	23.01327737974484	33.42629194177329	20.825226218609743
19	23.20222779498077	24.094121929221206	32.002071496053716	20.701578779744302
20	24.046616690263164	24.197295279183606	31.725872022224422	20.030216008328804
21	23.306070232815816	24.397352553105506	32.47177118265296	19.82480603142572
22	22.23111365661875	22.548930396126785	32.37341526537364	22.846540681880832
23	21.218783705301586	24.584429522298024	32.613483994079914	21.583302778320483
24	21.236581442714034	23.79182802835862	33.15036010309306	21.821230425834283
25	22.095690271194695	25.025759883096967	31.137074694293755	21.741475151414587
26	20.877682707825386	25.752924583091346	31.29310598619538	22.07628672288789
27	21.97485300139438	24.650535404115693	31.888192740128947	21.48641885436098
28	20.615667896896237	25.447686695589105	32.0763402499177	21.860305157596958
29	21.290911377973092	25.473379669898804	31.47563315785389	21.760075794274215
30	22.025302226992075	25.216316109227254	31.358007509842285	21.400374153938383
31	22.380454069793895	26.410370326755757	29.584255559451133	21.624920043999218
32	21.957858169429105	27.307884799126438	29.596432958733338	21.137824072711116
33	22.13463118538284	26.11356294644888	29.12994489392281	22.621860974245468
34	21.548510208942762	26.468714789250704	29.48978035183317	22.49299464997337
35	21.72568467762008	26.33650302561536	28.931493430895266	23.006318865869293
36	21.31914688619886	27.908993343913842	27.689131068961547	23.08272870092575
37	21.776802991090427	27.539523020637347	28.422317559809766	22.261356428462463
38	20.701043509446183	26.84715089002069	28.549444255612975	23.902361344920152
39	22.287986125793875	23.98853986291728	28.680317843503023	25.04315616778583
40	22.92361960480994	23.99175148470599	29.899262129894044	23.185366780590027
41	20.70238168519148	25.539619369291007	29.307119362600133	24.450879582917384
42	21.22159387436671	26.645086352480845	29.080700026495883	23.052619746656568
43	21.53124774182843	25.753861306113052	29.813485064620505	22.90140588743801
44	22.413774645851788	25.71130731741261	29.161659659086347	22.713258377649254
45	21.98274823829163	24.484200158975277	29.38071902859146	24.152332574141628
46	22.06772239811799	25.322433445829308	28.984619007983557	23.625225148069145
47	21.082155961706764	25.582307175565983	30.411783440342788	22.92375342238447
48	20.743062227848508	25.498135921186798	29.9473026391502	23.811499211814485
49	21.306300399044005	24.370990490923152	30.501173580128626	23.821535529904214
50	20.050021009359202	24.22633369285655	31.359479503162106	24.36416579462214
51	20.51931924323485	24.116737099316726	29.63028880508935	25.733654852359074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	302.0
1	769.5
2	1237.0
3	10505.0
4	19773.0
5	12719.0
6	5665.0
7	5500.5
8	5336.0
9	5295.0
10	5254.0
11	5041.0
12	4828.0
13	4662.0
14	4496.0
15	4355.5
16	4215.0
17	3825.5
18	3436.0
19	3379.0
20	3322.0
21	3476.0
22	3630.0
23	3819.5
24	4009.0
25	4438.0
26	5467.0
27	6067.0
28	7170.0
29	8273.0
30	9456.0
31	10639.0
32	11574.5
33	12510.0
34	14866.0
35	17222.0
36	18762.5
37	20303.0
38	22690.0
39	25077.0
40	27625.5
41	30174.0
42	33018.5
43	35863.0
44	39196.5
45	42530.0
46	49559.0
47	56588.0
48	61578.5
49	66569.0
50	66257.0
51	65945.0
52	58895.5
53	51846.0
54	47015.0
55	42184.0
56	39361.0
57	36538.0
58	35057.0
59	33576.0
60	31375.0
61	29174.0
62	27133.0
63	25092.0
64	22733.0
65	20374.0
66	16850.5
67	13327.0
68	11336.5
69	9346.0
70	7937.5
71	6529.0
72	5631.0
73	4733.0
74	3957.5
75	2373.5
76	1565.0
77	1265.0
78	965.0
79	690.0
80	415.0
81	300.0
82	185.0
83	132.0
84	79.0
85	57.5
86	36.0
87	21.5
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	747286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.94895882469363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32535332732859	26.359701075702475
2	8.004589826794984	5.755133401840286
3	3.281898956239482	3.539425513339743
4	2.034108716589431	2.9249636199049545
5	1.5759324517062672	2.832656540844354
6	1.348705940546247	2.9090744593990028
7	1.1579193624507287	2.913819683813972
8	1.0610773317020963	3.051570024573955
9	0.9633105422586543	3.116700991714469
>10	7.160660017762669	40.26453849244426
>50	0.049022088059071774	1.179391047654814
>100	0.032556651548754666	2.1123300061598034
>500	0.0037421438561786257	0.9792160307500346
>1k	7.484287712357252E-4	0.31317693633549665
>5k	0.0	0.0
>10k+	3.742143856178626E-4	1.748302175522386
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12996	1.7390931985879572	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	1212	0.16218690032999414	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCC	1116	0.14934041317514313	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	847	0.11334348562665432	No Hit
CTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGCT	840	0.11240676260494643	TruSeq Adapter, Index 14 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTT	825	0.11039949898700097	No Hit
CCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	821	0.10986422868888217	TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTG	756	0.10116608634445179	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3381757452969815E-4	0.0	0.0	0.11842855345878285	0.0
2	1.3381757452969815E-4	0.0	0.0	0.49873810027218496	0.0
3	1.3381757452969815E-4	0.0	0.0	0.7836357164459122	0.0
4	1.3381757452969815E-4	0.0	0.0	1.1608674590451313	0.0
5	2.676351490593963E-4	0.0	0.0	2.183233728452025	0.0
6	2.676351490593963E-4	0.0	0.0	3.164250367329242	0.0
7	2.676351490593963E-4	0.0	0.0	3.937849765685427	0.0
8	2.676351490593963E-4	0.0	0.0	5.069812628632143	0.0
9	2.676351490593963E-4	0.0	0.0	5.698889046496254	0.0
10	2.676351490593963E-4	0.0	0.0	6.765950385796067	0.0
11	2.676351490593963E-4	0.0	0.0	7.920126966114713	0.0
12	2.676351490593963E-4	0.0	0.0	8.843066777646042	0.0
13	2.676351490593963E-4	0.0	0.0	9.247998758172908	0.0
14	2.676351490593963E-4	0.0	0.0	9.467325762827084	0.0
15	2.676351490593963E-4	0.0	0.0	9.758780440152766	0.0
16	2.676351490593963E-4	0.0	0.0	10.237981174543615	0.0
17	2.676351490593963E-4	0.0	0.0	10.830659212135648	0.0
18	2.676351490593963E-4	0.0	0.0	11.501085260529436	0.0
19	2.676351490593963E-4	0.0	0.0	11.904679065311006	0.0
20	4.014527235890944E-4	0.0	0.0	12.297701281704729	0.0
21	4.014527235890944E-4	0.0	0.0	12.737425831609317	0.0
22	4.014527235890944E-4	0.0	0.0	13.216626566000166	0.0
23	4.014527235890944E-4	0.0	0.0	13.70666652392792	0.0
24	4.014527235890944E-4	0.0	0.0	14.087377523464912	0.0
25	4.014527235890944E-4	0.0	0.0	14.4338312239223	0.0
26	4.014527235890944E-4	0.0	0.0	14.740942557467957	0.0
27	4.014527235890944E-4	0.0	0.0	15.087128622776287	0.0
28	4.014527235890944E-4	0.0	0.0	15.416453673693873	0.0
29	4.014527235890944E-4	0.0	0.0	15.766788083812623	0.0
30	4.014527235890944E-4	0.0	0.0	16.19393378171142	0.0
31	4.014527235890944E-4	0.0	0.0	16.56086157107185	0.0
32	4.014527235890944E-4	0.0	0.0	16.919626488385973	0.0
33	4.014527235890944E-4	0.0	0.0	17.265411100970713	0.0
34	4.014527235890944E-4	0.0	0.0	17.604371017254437	0.0
35	4.014527235890944E-4	0.0	0.0	17.967953367251628	0.0
36	4.014527235890944E-4	0.0	0.0	18.32645064941669	0.0
37	4.014527235890944E-4	0.0	0.0	18.667685464467418	0.0
38	4.014527235890944E-4	0.0	0.0	19.03541615927503	0.0
39	4.014527235890944E-4	0.0	0.0	19.46898510075125	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	25	3.889492E-5	45.0	1
GACGCAT	20	7.0317E-4	45.0	9
CGTTTTT	5875	0.0	43.314896	1
CGTAAGG	45	1.9266736E-8	40.0	2
ACATACG	40	3.4560617E-7	39.375	17
TATAGCG	35	6.2453546E-6	38.57143	1
AACACGT	70	0.0	38.57143	41
TCGCAAC	30	1.13954E-4	37.499996	16
ACCGGGC	60	1.546141E-10	37.499996	4
TATTGCG	30	1.13954E-4	37.499996	1
CACTAGG	60	1.546141E-10	37.499996	2
CGGGCTA	30	1.13954E-4	37.499996	6
GCGTAAG	30	1.13954E-4	37.499996	1
GTTTTTT	7160	0.0	36.861034	2
TCGGCGT	160	0.0	36.5625	4
TTAGGCG	50	4.8750735E-8	36.0	1
ACCGGTT	25	0.0021066382	36.0	39
GACCGGT	50	4.8750735E-8	36.0	3
TCTACGG	25	0.0021066382	36.0	2
ACTAGGG	270	0.0	35.833332	3
>>END_MODULE