FastQCFastQC Report
Sat 18 Jun 2016
SRR3551605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744863
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70390.9450059943909149No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCG65780.8831154185400536No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC65700.8820413955317957No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTC56060.7526216230367195No Hit
GCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC29500.3960459842950985No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTACGAAGT14630.19641195763516242No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCT14150.18996781958561507No Hit
GAACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCT13410.18003310675922957No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTA12790.17170942844523088No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA10750.14432184173465457No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT10600.142308048594171No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTC10060.13505839328843022No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC8770.11773977228027167No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG7990.1072680479497572No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG207.031686E-445.040
CGGCACA207.031686E-445.09
CGCGTCA207.031686E-445.039
CGATCGA207.031686E-445.041
CGAATAT1050.042.85714314
CGAAAGG950.042.631582
CGTTTTT29350.042.3168681
TACGGCT9400.041.8882987
ACACGAC1400.041.78571726
AAACACG1350.041.66666440
ACGGCTG9600.041.2499968
TAGCGCG556.002665E-1140.9090921
GTTAGCG556.002665E-1140.9090921
GCTACGA1100.040.90909210
CACGACG451.9266736E-840.00000426
CGTCATA451.9266736E-840.00000438
TCGACAA403.4560435E-739.37519
CTACGAA1150.039.13043611
CGAGACA1500.039.022
CCGATGA6800.038.71323418