##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551605_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744863
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02494552689555	33.0	31.0	34.0	30.0	34.0
2	32.12548884828485	33.0	31.0	34.0	30.0	34.0
3	32.09154703616638	33.0	31.0	34.0	30.0	34.0
4	35.73835591242953	37.0	35.0	37.0	35.0	37.0
5	35.78680911791833	37.0	35.0	37.0	35.0	37.0
6	35.69252332308089	37.0	35.0	37.0	35.0	37.0
7	36.00393897938279	37.0	35.0	37.0	35.0	37.0
8	35.795873872108025	37.0	35.0	37.0	35.0	37.0
9	37.65522518906161	39.0	38.0	39.0	35.0	39.0
10	37.244262367710576	39.0	37.0	39.0	34.0	39.0
11	37.330766865853185	39.0	37.0	39.0	34.0	39.0
12	37.29771246524529	39.0	37.0	39.0	34.0	39.0
13	37.35318709615057	39.0	37.0	39.0	34.0	39.0
14	38.46098678548941	40.0	38.0	41.0	34.0	41.0
15	38.49239927342344	40.0	38.0	41.0	34.0	41.0
16	38.32555785426313	40.0	38.0	41.0	34.0	41.0
17	38.29700763764612	40.0	38.0	41.0	34.0	41.0
18	38.11486407567566	40.0	37.0	41.0	34.0	41.0
19	37.91665984214547	40.0	37.0	41.0	34.0	41.0
20	38.07704235543986	40.0	37.0	41.0	34.0	41.0
21	38.021009232570286	40.0	37.0	41.0	34.0	41.0
22	38.19626562200029	40.0	37.0	41.0	34.0	41.0
23	38.194462605875174	40.0	37.0	41.0	34.0	41.0
24	38.08319516474842	40.0	37.0	41.0	34.0	41.0
25	37.77224268086883	40.0	36.0	41.0	33.0	41.0
26	37.93679643102154	40.0	36.0	41.0	34.0	41.0
27	37.98044741113466	40.0	36.0	41.0	34.0	41.0
28	37.91126287652897	40.0	36.0	41.0	34.0	41.0
29	37.94264448630151	40.0	36.0	41.0	34.0	41.0
30	37.637338678387835	40.0	36.0	41.0	33.0	41.0
31	37.51391732439388	40.0	36.0	41.0	33.0	41.0
32	37.45865078544645	40.0	36.0	41.0	33.0	41.0
33	37.36445628256471	40.0	36.0	41.0	33.0	41.0
34	37.158681529355064	40.0	36.0	41.0	32.0	41.0
35	37.07966834169505	40.0	36.0	41.0	32.0	41.0
36	36.920145046807264	40.0	35.0	41.0	31.0	41.0
37	36.883330223141705	40.0	35.0	41.0	31.0	41.0
38	36.7404515998244	39.0	35.0	41.0	31.0	41.0
39	36.69112709317015	39.0	35.0	41.0	31.0	41.0
40	36.59335609367092	39.0	35.0	41.0	31.0	41.0
41	36.40853552935238	39.0	35.0	41.0	30.0	41.0
42	36.480155411129296	39.0	35.0	41.0	30.0	41.0
43	36.45746533255109	39.0	35.0	41.0	31.0	41.0
44	36.53151116379791	39.0	35.0	41.0	31.0	41.0
45	36.541300883518176	39.0	35.0	41.0	31.0	41.0
46	36.44879125423064	39.0	35.0	41.0	31.0	41.0
47	36.338303822313634	39.0	35.0	41.0	30.0	41.0
48	36.308824575794475	39.0	35.0	41.0	31.0	41.0
49	36.305732732059454	39.0	35.0	41.0	31.0	41.0
50	36.13641031975008	39.0	35.0	41.0	30.0	41.0
51	35.32791936235254	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	19.0
10	23.0
11	26.0
12	30.0
13	36.0
14	31.0
15	45.0
16	66.0
17	110.0
18	206.0
19	465.0
20	922.0
21	1555.0
22	2044.0
23	2679.0
24	3740.0
25	5360.0
26	7135.0
27	8608.0
28	8978.0
29	8961.0
30	9925.0
31	12075.0
32	15571.0
33	21816.0
34	38501.0
35	51698.0
36	53691.0
37	82924.0
38	159225.0
39	248286.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45971943833967	18.62355896319189	23.679522274565926	13.237199323902516
2	33.36089992387862	26.37693105980563	24.51645470375089	15.745714312564859
3	30.987040569876605	25.669284150239708	27.995752239002343	15.347923040881343
4	26.410628531689724	23.947759520878336	31.843842424714342	17.797769522717598
5	27.50465521847642	27.482234988179034	26.04411818012171	18.968991613222837
6	24.990904367648817	35.4206075479652	25.171742991664235	14.41674509272175
7	76.41620002604506	5.15155135910899	14.371501873498884	4.060746741347066
8	77.31663406559328	7.729743590432066	10.438295364382444	4.515326979592221
9	71.60054936276873	7.119967027493647	12.096049877628504	9.183433732109126
10	36.23632802273707	27.85653200655691	19.939774159811936	15.967365810894085
11	28.29607592268645	24.586131946411623	28.106510861728935	19.01128126917299
12	25.232962303134936	21.532550281058395	31.82934311410286	21.405144301703803
13	24.643860683105483	22.74753880915014	33.71237395333102	18.89622655441336
14	20.8137603827818	28.045828561762363	30.045659403138565	21.094751652317274
15	18.408351602912212	24.707630799220794	35.3714709953374	21.512546602529593
16	20.605131413427706	23.084110769362955	33.01345348070719	23.297304336502147
17	20.395294168189317	23.88506342777128	32.240559673389605	23.479082730649797
18	21.16536866511023	23.474383879988668	31.860489781342338	23.49975767355876
19	22.454464780771765	25.419305295067684	29.536169738596225	22.590060185564326
20	24.183104812562846	24.7438790757495	31.375165634485803	19.697850477201847
21	22.581333748622228	27.02121061188433	30.22354446388128	20.17391117561216
22	21.261225218597247	22.628993519613676	30.997109535579025	25.112671726210056
23	20.94680498292975	26.895818425670225	30.44506170933447	21.712314882065563
24	21.584908902710968	25.345331960373922	30.16850078470806	22.901258352207048
25	20.910556706401042	28.09832143629097	28.49705247810671	22.494069379201274
26	20.439866123032022	26.036197260435813	30.30785527002952	23.216081346502644
27	22.304772823995823	25.94866438526279	29.757015719669255	21.98954707107213
28	19.782295536226123	26.31396646094651	32.092210245373984	21.811527757453383
29	22.22891994903761	23.735774229623434	30.98838309863693	23.046922722702025
30	22.011967301369513	25.597190355810394	31.479345866286824	20.911496476533266
31	22.539715357052238	27.013692450826525	26.975296128281308	23.471296063839926
32	23.129756747213918	27.572989932376828	27.312807858626353	21.984445461782904
33	23.74154710329282	25.349225293778854	27.27374027170097	23.635487331227353
34	22.626174209216998	26.766264400299118	27.999377066655214	22.608184323828677
35	22.364381100954137	25.51784690607535	28.36239684344638	23.75537514952414
36	24.66037378685745	27.52935774766635	27.12257153328867	20.68769693218753
37	20.607547965196286	27.278170616610037	29.564631348315057	22.54965006987862
38	21.74225327342075	28.50067730575958	26.251404620715483	23.50566480010418
39	23.50459077709592	25.52711035452157	26.952741645107892	24.015557223274616
40	22.981273066322263	25.805819325164492	29.837701698164633	21.375205910348612
41	21.21235717172151	27.557282345881056	28.196057530042438	23.034302952354995
42	21.999884542526612	27.238566018180528	29.058363752797494	21.70318568649537
43	23.852037220267352	25.62981380468623	27.564532001186798	22.95361697385962
44	22.201800868079097	24.851549882327355	29.726943075438033	23.219706174155515
45	21.923494656064268	24.346087804065984	28.555452479180737	25.174965060689015
46	24.32608412553718	26.108291054865123	27.82471407493727	21.74091074466043
47	20.54203256169255	26.37209795626847	31.446856670287023	21.63901281175196
48	21.521407292347718	26.44190945180523	28.487117765280328	23.54956549056672
49	21.56114614365326	24.377905735685623	31.089878272917304	22.971069847743813
50	21.72063856037956	23.484855604319183	30.154404232724673	24.640101602576582
51	21.192084987440644	23.86116641583754	28.47543776506552	26.471310831656293
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	216.0
1	673.0
2	1130.0
3	6189.5
4	11249.0
5	7412.5
6	3576.0
7	3490.0
8	3404.0
9	3294.5
10	3185.0
11	3111.5
12	3038.0
13	2864.0
14	2690.0
15	2452.5
16	2215.0
17	2206.0
18	2197.0
19	2164.0
20	2131.0
21	2296.0
22	2461.0
23	2684.5
24	2908.0
25	3587.0
26	5197.0
27	6128.0
28	7303.0
29	8478.0
30	9855.0
31	11232.0
32	12886.5
33	14541.0
34	16968.5
35	19396.0
36	20799.0
37	22202.0
38	24147.5
39	26093.0
40	27863.5
41	29634.0
42	33153.5
43	36673.0
44	41122.5
45	45572.0
46	63665.0
47	81758.0
48	77587.0
49	73416.0
50	71743.5
51	70071.0
52	61319.0
53	52567.0
54	47591.5
55	42616.0
56	38944.5
57	35273.0
58	33036.5
59	30800.0
60	29108.5
61	27417.0
62	24860.5
63	22304.0
64	18793.0
65	15282.0
66	12312.0
67	9342.0
68	7870.5
69	6399.0
70	5644.5
71	4890.0
72	4288.5
73	3687.0
74	2945.5
75	1587.0
76	970.0
77	840.0
78	710.0
79	512.0
80	314.0
81	228.0
82	142.0
83	105.0
84	68.0
85	40.0
86	12.0
87	7.0
88	2.0
89	1.0
90	0.0
91	1.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	744863.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.032870522762906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52415805610988	22.816656772504135
2	9.211987097218689	5.717488056907002
3	3.26466971635295	3.039362178214986
4	1.73825497795165	2.1577216666528662
5	1.163414790972826	1.8052050286263492
6	0.9583981897467931	1.7845108158997554
7	0.7999789455794826	1.7377950127373136
8	0.6866861219536858	1.7047873209893531
9	0.5989483432778877	1.6728377748089436
>10	7.9016284274300075	47.597058675268364
>50	0.10444149004132659	2.186269972138555
>100	0.04003590449143521	2.251632542354604
>500	0.0021758643745345223	0.4803608800548988
>1k	0.0034813829992552357	1.5650554509294974
>5k	0.0017406914996276179	3.4832578519134114
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7039	0.9450059943909149	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCG	6578	0.8831154185400536	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC	6570	0.8820413955317957	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTC	5606	0.7526216230367195	No Hit
GCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC	2950	0.3960459842950985	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTACGAAGT	1463	0.19641195763516242	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCT	1415	0.18996781958561507	No Hit
GAACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCT	1341	0.18003310675922957	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTA	1279	0.17170942844523088	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	1075	0.14432184173465457	No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT	1060	0.142308048594171	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTC	1006	0.13505839328843022	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC	877	0.11773977228027167	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	799	0.1072680479497572	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.342528760322368E-4	0.0	0.0	0.1484836808916539	0.0
2	1.342528760322368E-4	0.0	0.0	0.7923604743422616	0.0
3	1.342528760322368E-4	0.0	0.0	0.9928000182583911	0.0
4	1.342528760322368E-4	0.0	0.0	1.5018869241726331	0.0
5	1.342528760322368E-4	0.0	0.0	3.147021667071663	0.0
6	1.342528760322368E-4	0.0	0.0	3.8039209894973975	0.0
7	1.342528760322368E-4	0.0	0.0	4.5368074397573785	0.0
8	1.342528760322368E-4	0.0	0.0	5.578341252015472	0.0
9	1.342528760322368E-4	0.0	0.0	5.967406086756894	0.0
10	1.342528760322368E-4	0.0	0.0	7.7194061189775836	0.0
11	1.342528760322368E-4	0.0	0.0	8.940436026490778	0.0
12	1.342528760322368E-4	0.0	0.0	10.823466865718931	0.0
13	1.342528760322368E-4	0.0	0.0	11.263682046228634	0.0
14	1.342528760322368E-4	0.0	0.0	11.446400210508509	0.0
15	1.342528760322368E-4	0.0	0.0	11.908767115563533	0.0
16	1.342528760322368E-4	0.0	0.0	12.545931265212529	0.0
17	1.342528760322368E-4	0.0	0.0	13.29385403758812	0.0
18	1.342528760322368E-4	0.0	0.0	14.09145037409564	0.0
19	1.342528760322368E-4	0.0	0.0	14.769561650934467	0.0
20	1.342528760322368E-4	0.0	0.0	15.257973613939745	0.0
21	1.342528760322368E-4	0.0	0.0	15.8225069576553	0.0
22	1.342528760322368E-4	0.0	0.0	16.45268995775062	0.0
23	1.342528760322368E-4	0.0	0.0	17.04702743994533	0.0
24	1.342528760322368E-4	0.0	0.0	17.500802160934292	0.0
25	1.342528760322368E-4	0.0	0.0	17.910676191460713	0.0
26	1.342528760322368E-4	0.0	0.0	18.294370911160843	0.0
27	1.342528760322368E-4	0.0	0.0	18.699680343902166	0.0
28	2.685057520644736E-4	0.0	0.0	19.103244489255072	0.0
29	2.685057520644736E-4	0.0	0.0	19.515669324426103	0.0
30	2.685057520644736E-4	0.0	0.0	19.9729346201919	0.0
31	2.685057520644736E-4	0.0	0.0	20.408182444288414	0.0
32	2.685057520644736E-4	0.0	0.0	20.8179222219388	0.0
33	2.685057520644736E-4	0.0	0.0	21.23383763188667	0.0
34	2.685057520644736E-4	0.0	0.0	21.632837179454476	0.0
35	2.685057520644736E-4	0.0	0.0	22.07238109558402	0.0
36	2.685057520644736E-4	0.0	0.0	22.497559953978115	0.0
37	2.685057520644736E-4	0.0	0.0	22.937506628735754	0.0
38	2.685057520644736E-4	0.0	0.0	23.340802268336592	0.0
39	4.027586280967104E-4	0.0	0.0	23.748393999970464	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	20	7.031686E-4	45.0	40
CGGCACA	20	7.031686E-4	45.0	9
CGCGTCA	20	7.031686E-4	45.0	39
CGATCGA	20	7.031686E-4	45.0	41
CGAATAT	105	0.0	42.857143	14
CGAAAGG	95	0.0	42.63158	2
CGTTTTT	2935	0.0	42.316868	1
TACGGCT	940	0.0	41.888298	7
ACACGAC	140	0.0	41.785717	26
AAACACG	135	0.0	41.666664	40
ACGGCTG	960	0.0	41.249996	8
TAGCGCG	55	6.002665E-11	40.909092	1
GTTAGCG	55	6.002665E-11	40.909092	1
GCTACGA	110	0.0	40.909092	10
CACGACG	45	1.9266736E-8	40.000004	26
CGTCATA	45	1.9266736E-8	40.000004	38
TCGACAA	40	3.4560435E-7	39.375	19
CTACGAA	115	0.0	39.130436	11
CGAGACA	150	0.0	39.0	22
CCGATGA	680	0.0	38.713234	18
>>END_MODULE