FastQCFastQC Report
Sat 18 Jun 2016
SRR3551600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606394
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC29380.4845034746386013No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.4333815967836094No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT11280.18601767167880948Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC9570.1578181842168623No Hit
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC9480.1563340006662335No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCC9230.15221126858115352No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA8830.14561489724502552No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGC6970.11494177053203032No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTC6870.11329267769799835No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCG6850.11296285913119193No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6370.10504721352783833No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTT502.1827873E-1145.031
ACGTGCG253.8886174E-545.01
ACTACCG207.030647E-445.01
TCGTAAG253.8886174E-545.01
ATAACGC253.8886174E-545.011
CGTAAGG1100.042.9545442
CGAATAT1050.042.8571414
TCGTGTA650.041.5384617
GCGTAAG603.6379788E-1241.2500041
TATTAGT1600.040.7812530
TATACTG1250.039.618
GATACGC403.4546247E-739.37511
GGCGATA2700.039.1666688
CGTTTTT11900.038.7605061
TGCGTAG356.2434083E-638.5714261
GCATATT1700.038.38235527
GTCCCCC1900.037.8947379
GATTCAT950.037.89473745
GCCCCGT301.1392856E-437.50000422
AACTTCG601.546141E-1037.50000411