##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551600_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	606394
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0183362632216	33.0	31.0	34.0	30.0	34.0
2	32.09000089051013	33.0	31.0	34.0	30.0	34.0
3	32.06054149612298	33.0	31.0	34.0	30.0	34.0
4	35.709967446907456	37.0	35.0	37.0	33.0	37.0
5	35.76998947878772	37.0	35.0	37.0	35.0	37.0
6	35.660026319521634	37.0	35.0	37.0	33.0	37.0
7	35.97130413559501	37.0	35.0	37.0	35.0	37.0
8	35.78348235635577	37.0	35.0	37.0	35.0	37.0
9	37.5975586829685	39.0	37.0	39.0	35.0	39.0
10	37.212111267591695	39.0	37.0	39.0	34.0	39.0
11	37.33236311704931	39.0	37.0	39.0	34.0	39.0
12	37.281109311767594	39.0	37.0	39.0	34.0	39.0
13	37.34126656926025	39.0	37.0	39.0	34.0	39.0
14	38.38715422646003	40.0	38.0	41.0	34.0	41.0
15	38.48313967486486	40.0	38.0	41.0	34.0	41.0
16	38.250888036491126	40.0	37.0	41.0	34.0	41.0
17	38.28920470848986	40.0	37.0	41.0	34.0	41.0
18	38.0996860127244	40.0	37.0	41.0	34.0	41.0
19	37.93217610992193	40.0	37.0	41.0	34.0	41.0
20	38.22999567937678	40.0	37.0	41.0	34.0	41.0
21	38.089049363944895	40.0	37.0	41.0	34.0	41.0
22	38.250075033723945	40.0	37.0	41.0	34.0	41.0
23	38.2944158418454	40.0	37.0	41.0	34.0	41.0
24	38.16119222815529	40.0	37.0	41.0	34.0	41.0
25	37.77855651606051	40.0	36.0	41.0	33.0	41.0
26	38.00146109625095	40.0	37.0	41.0	34.0	41.0
27	38.08431646751122	40.0	37.0	41.0	34.0	41.0
28	38.00579491221879	40.0	37.0	41.0	34.0	41.0
29	38.04122896994363	40.0	37.0	41.0	34.0	41.0
30	37.737601295527334	40.0	36.0	41.0	33.0	41.0
31	37.666850265668856	40.0	36.0	41.0	33.0	41.0
32	37.70705349986972	40.0	36.0	41.0	33.0	41.0
33	37.676472722355435	40.0	36.0	41.0	33.0	41.0
34	37.60978175905434	40.0	36.0	41.0	33.0	41.0
35	37.5642008331217	40.0	36.0	41.0	33.0	41.0
36	37.40191030913894	40.0	36.0	41.0	33.0	41.0
37	37.43414512676576	40.0	36.0	41.0	33.0	41.0
38	37.262359126244654	39.0	35.0	41.0	33.0	41.0
39	37.21466076511311	39.0	35.0	41.0	33.0	41.0
40	37.1591621948766	39.0	35.0	41.0	33.0	41.0
41	36.986894659247945	39.0	35.0	41.0	32.0	41.0
42	37.05184253142346	39.0	35.0	41.0	33.0	41.0
43	37.03754984383091	39.0	35.0	41.0	33.0	41.0
44	37.07092253551321	39.0	35.0	41.0	33.0	41.0
45	37.03701553775268	39.0	35.0	41.0	33.0	41.0
46	36.94150502808405	39.0	35.0	41.0	33.0	41.0
47	36.91379861937948	39.0	35.0	41.0	32.0	41.0
48	36.856339277763304	39.0	35.0	41.0	32.0	41.0
49	36.820140040963466	39.0	35.0	41.0	32.0	41.0
50	36.72318492597222	39.0	35.0	41.0	32.0	41.0
51	35.877617522600815	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	21.0
10	20.0
11	18.0
12	31.0
13	32.0
14	33.0
15	55.0
16	54.0
17	78.0
18	152.0
19	232.0
20	378.0
21	549.0
22	839.0
23	1200.0
24	1827.0
25	2755.0
26	3928.0
27	4934.0
28	5490.0
29	6173.0
30	7291.0
31	9194.0
32	12371.0
33	17806.0
34	34089.0
35	46080.0
36	46635.0
37	73605.0
38	136135.0
39	194282.0
40	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80881077319367	20.58793457718909	23.84142982945082	11.761824820166426
2	33.210420947436816	24.46726055996596	24.896684333947896	17.425634158649327
3	32.77324643713494	23.72286005468392	26.75521195790196	16.74868155027919
4	29.54267357526625	25.00931737451228	26.05401768487155	19.393991365349923
5	25.334683390666797	29.056191189226805	24.533224273327242	21.07590114677916
6	26.328756551021282	34.48220134104229	24.00271110861915	15.186330999317278
7	77.96218300312998	6.741491505522812	10.297430383546011	4.998895107801198
8	78.33388852792079	6.038813048941777	9.64389489341913	5.983403529718302
9	71.91347539718402	7.674548231018117	12.277001421518023	8.134974950279851
10	37.3789978133029	25.30516462893762	20.03664284277219	17.279194714987288
11	29.199002628653975	24.915154173689054	25.21331015808204	20.67253303957493
12	26.533738790291462	21.777919966226577	30.651523596869364	21.036817646612597
13	24.1753711283423	24.284046346105008	31.813144589161503	19.72743793639119
14	20.660659571169898	27.92623277934808	29.532779018262055	21.880328631219967
15	19.686210615540393	25.104634940319333	33.700201519144315	21.50895292499596
16	21.42039663980844	23.789813223745618	31.620200727579757	23.16958940886618
17	21.26422754842561	24.406903762240393	28.964501627654627	25.36436706167937
18	22.77562113081594	24.245787392355464	30.034927786224795	22.9436636906038
19	24.362708074288335	25.42967113790704	27.567719997229524	22.639900790575105
20	24.848530823194164	25.540325267070585	27.997803408345067	21.613340501390184
21	24.50782824368315	26.33667219662464	28.01775743163686	21.137742128055358
22	22.031550444100702	24.68708463474243	27.06969396135186	26.211670959805012
23	21.438536660982795	26.191881845796626	28.49335580497169	23.876225688248894
24	22.614669670214415	25.33946575988549	28.964336718371225	23.081527851528875
25	22.42881690781901	26.174566371039294	27.94173425198798	23.454882469153716
26	20.89153256793438	26.20969204840417	27.32332443922598	25.575450944435467
27	23.167115769615133	25.75025478484286	28.273861548762024	22.80876789677998
28	21.111191733427443	25.54444799915567	29.203784997872674	24.14057526954422
29	21.593221568814993	25.347711224055647	28.363902017500177	24.695165189629183
30	22.63660260490704	26.68792897027345	27.558485077358945	23.11698334746056
31	24.525803355574098	26.901651401563996	24.395854840252376	24.176690402609523
32	23.17140341098362	26.987239319650257	26.20721840915313	23.634138860212996
33	22.507148817435528	26.969923844892925	25.933139180137005	24.58978815753454
34	21.69299168527393	25.087979102695606	27.193375923904263	26.0256532881262
35	22.389073770518838	24.688898636859864	27.230150694103173	25.691876898518125
36	23.006329218297015	27.015933534962418	26.714974092751575	23.262763153988992
37	22.373407388595535	27.031435007602315	27.821680293670454	22.773477310131696
38	22.734228900681735	27.510001748038405	26.649999835090714	23.105769516189145
39	22.079374136287626	25.106613851720166	28.472082507412672	24.341929504579532
40	22.95438279402501	23.5775749760057	29.451313832260873	24.01672839770842
41	21.119272288314196	24.95473240170582	27.27731474915649	26.64868056082349
42	22.75104964758886	25.08616510057817	28.310801228244344	23.851984023588624
43	22.808273168929773	24.292621628841975	29.46714512346758	23.431960078760675
44	22.886110350696082	23.809932156320805	28.27963337368114	25.024324119301973
45	22.575751079331262	24.056141716441783	27.257525635148106	26.11058156907885
46	23.323119951714563	25.19319122550685	27.694700145449985	23.7889886773286
47	21.608228313604684	25.10710857957038	29.50886717216859	23.775795934656347
48	21.53929623314215	24.53256463619363	29.273706534035625	24.654432596628595
49	22.027757530582427	22.970214085231714	30.997503273449272	24.004525110736584
50	21.000537604263894	22.809427533913595	30.68796854850147	25.50206631332104
51	21.343713823025954	22.783206957852485	27.484275899827505	28.388803319294055
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	261.0
1	655.0
2	1049.0
3	2662.5
4	4276.0
5	2909.0
6	1542.0
7	1519.0
8	1496.0
9	1480.5
10	1465.0
11	1456.0
12	1447.0
13	1434.0
14	1421.0
15	1411.0
16	1401.0
17	1334.0
18	1267.0
19	1340.5
20	1414.0
21	1577.0
22	1740.0
23	1851.5
24	1963.0
25	2541.0
26	3942.0
27	4765.0
28	5366.0
29	5967.0
30	6818.5
31	7670.0
32	9287.5
33	10905.0
34	12215.0
35	13525.0
36	13888.5
37	14252.0
38	16172.0
39	18092.0
40	20505.0
41	22918.0
42	26587.5
43	30257.0
44	34348.5
45	38440.0
46	46430.5
47	54421.0
48	60509.5
49	66598.0
50	64863.5
51	63129.0
52	54593.0
53	46057.0
54	41180.0
55	36303.0
56	33552.5
57	30802.0
58	28951.5
59	27101.0
60	25655.5
61	24210.0
62	22403.0
63	20596.0
64	17771.5
65	14947.0
66	12758.0
67	10569.0
68	8776.0
69	6983.0
70	6292.5
71	5602.0
72	4614.5
73	3627.0
74	2905.0
75	1627.5
76	1072.0
77	896.5
78	721.0
79	607.5
80	494.0
81	371.0
82	248.0
83	151.5
84	55.0
85	32.0
86	9.0
87	11.0
88	13.0
89	6.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	606394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.13497488134248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.34183719110938	21.800194466242477
2	10.333268855188017	6.227855947864989
3	3.7279030774386	3.3702079679607464
4	1.881036898110094	2.267399987017042
5	1.2224082678906356	1.8418621223814837
6	0.8904144320588756	1.609956992644743
7	0.68043756293328	1.4353478207121397
8	0.5888353385623691	1.4195630509459032
9	0.5136623092479453	1.3931280708011282
>10	7.613016925877014	49.62647562541226
>50	0.13279301362153134	2.660060595893757
>100	0.06612100263312592	3.7981414525888
>500	0.006612100263312592	1.438292439552513
>1k	0.001653025065828148	1.1115134599820506
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	2938	0.4845034746386013	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2628	0.4333815967836094	No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT	1128	0.18601767167880948	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	957	0.1578181842168623	No Hit
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	948	0.1563340006662335	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCC	923	0.15221126858115352	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	883	0.14561489724502552	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGC	697	0.11494177053203032	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTC	687	0.11329267769799835	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCG	685	0.11296285913119193	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	637	0.10504721352783833	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.947278502095997E-4	0.0	0.0	0.1934385894319535	0.0
2	4.947278502095997E-4	0.0	0.0	1.1141271186720185	0.0
3	4.947278502095997E-4	0.0	0.0	1.4942430169163943	0.0
4	4.947278502095997E-4	0.0	0.0	2.097646084888703	0.0
5	4.947278502095997E-4	0.0	0.0	3.7546545645240554	0.0
6	4.947278502095997E-4	0.0	0.0	4.707170585460938	0.0
7	6.596371336127996E-4	0.0	0.0	5.603782359324136	0.0
8	6.596371336127996E-4	0.0	0.0	7.070320616628792	0.0
9	6.596371336127996E-4	0.0	0.0	7.657232756260781	0.0
10	6.596371336127996E-4	0.0	0.0	9.0136116122521	0.0
11	6.596371336127996E-4	0.0	0.0	10.67870064677421	0.0
12	6.596371336127996E-4	0.0	0.0	11.9923679983641	0.0
13	6.596371336127996E-4	0.0	0.0	12.494022038476635	0.0
14	6.596371336127996E-4	0.0	0.0	12.688120265042201	0.0
15	6.596371336127996E-4	0.0	0.0	13.108474028436957	0.0
16	6.596371336127996E-4	0.0	0.0	13.914880424278603	0.0
17	6.596371336127996E-4	0.0	0.0	14.9041712154144	0.0
18	6.596371336127996E-4	0.0	0.0	16.04006635949564	0.0
19	6.596371336127996E-4	0.0	0.0	16.664907634310367	0.0
20	6.596371336127996E-4	0.0	0.0	17.25511795961042	0.0
21	6.596371336127996E-4	0.0	0.0	17.92250582954317	0.0
22	6.596371336127996E-4	0.0	0.0	18.637717391662846	0.0
23	6.596371336127996E-4	0.0	0.0	19.34550803602938	0.0
24	6.596371336127996E-4	0.0	0.0	19.903561051065807	0.0
25	6.596371336127996E-4	0.0	0.0	20.41956219883442	0.0
26	6.596371336127996E-4	0.0	0.0	20.868280358974527	0.0
27	6.596371336127996E-4	0.0	0.0	21.329531624653278	0.0
28	6.596371336127996E-4	0.0	0.0	21.8151894642757	0.0
29	6.596371336127996E-4	0.0	0.0	22.273142544286387	0.0
30	6.596371336127996E-4	0.0	0.0	22.79590497267453	0.0
31	6.596371336127996E-4	0.0	0.0	23.280903175163342	0.0
32	6.596371336127996E-4	0.0	0.0	23.77084865615425	0.0
33	6.596371336127996E-4	0.0	0.0	24.241664660270384	0.0
34	6.596371336127996E-4	0.0	0.0	24.72072612855668	0.0
35	8.245464170159995E-4	0.0	0.0	25.218752164434346	0.0
36	8.245464170159995E-4	0.0	0.0	25.662523046072355	0.0
37	8.245464170159995E-4	0.0	0.0	26.117177940414976	0.0
38	8.245464170159995E-4	0.0	0.0	26.553527904299845	0.0
39	8.245464170159995E-4	0.0	0.0	26.999937334472307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTT	50	2.1827873E-11	45.0	31
ACGTGCG	25	3.8886174E-5	45.0	1
ACTACCG	20	7.030647E-4	45.0	1
TCGTAAG	25	3.8886174E-5	45.0	1
ATAACGC	25	3.8886174E-5	45.0	11
CGTAAGG	110	0.0	42.954544	2
CGAATAT	105	0.0	42.85714	14
TCGTGTA	65	0.0	41.53846	17
GCGTAAG	60	3.6379788E-12	41.250004	1
TATTAGT	160	0.0	40.78125	30
TATACTG	125	0.0	39.6	18
GATACGC	40	3.4546247E-7	39.375	11
GGCGATA	270	0.0	39.166668	8
CGTTTTT	1190	0.0	38.760506	1
TGCGTAG	35	6.2434083E-6	38.571426	1
GCATATT	170	0.0	38.382355	27
GTCCCCC	190	0.0	37.894737	9
GATTCAT	95	0.0	37.894737	45
GCCCCGT	30	1.1392856E-4	37.500004	22
AACTTCG	60	1.546141E-10	37.500004	11
>>END_MODULE