FastQCFastQC Report
Sat 18 Jun 2016
SRR3551599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388368
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC29890.7696308655707988Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23520.6056111729081696No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC17770.45755572034771147No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC17460.44957360029662585No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG17420.44854364932229224No Hit
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT12090.3113026819923371Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC10960.2822065669674123Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC9670.24899064804515306Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC7800.20084043999505624No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5310.13672599184278827No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5090.13106126148395336No Hit
ACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC5010.12900135953528613Illumina Single End Adapter 1 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4380.11277963168953156No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT4250.10943229102294731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAT351.209155E-745.041
CTCACGA351.209155E-745.024
GCGACGT351.209155E-745.017
TCACCGC207.0275116E-445.019
ACCGCCA207.0275116E-445.021
CGGTTGC207.0275116E-445.036
CGCATCG207.0275116E-445.021
GCAGTAA207.0275116E-445.01
AATTGCG207.0275116E-445.01
TCGCTAT351.209155E-745.023
TCATGCG207.0275116E-445.01
CGCAGTA207.0275116E-445.013
GCGATCA502.1827873E-1145.09
ATGAACG351.209155E-745.028
TTTGACG207.0275116E-445.01
CCTATGC800.045.035
TTCGCCC207.0275116E-445.027
TATCGCG207.0275116E-445.01
CTAGCGG253.8860195E-545.02
ACGGTAG253.8860195E-545.01