##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551599_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388368
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.936879454537966	33.0	31.0	34.0	30.0	34.0
2	32.009828307172576	33.0	31.0	34.0	30.0	34.0
3	31.984241750092696	33.0	31.0	34.0	30.0	34.0
4	35.636154369052036	37.0	35.0	37.0	33.0	37.0
5	35.70993233222099	37.0	35.0	37.0	35.0	37.0
6	35.60007518642113	37.0	35.0	37.0	33.0	37.0
7	35.907613397602276	37.0	35.0	37.0	35.0	37.0
8	35.648603901454294	37.0	35.0	37.0	35.0	37.0
9	37.45578935442673	39.0	37.0	39.0	34.0	39.0
10	37.17236487043217	39.0	37.0	39.0	34.0	39.0
11	37.26430601903349	39.0	37.0	39.0	34.0	39.0
12	37.156753388538704	39.0	37.0	39.0	34.0	39.0
13	37.16191086804268	39.0	37.0	39.0	34.0	39.0
14	38.189701520207635	40.0	37.0	41.0	33.0	41.0
15	38.27709543525728	40.0	37.0	41.0	34.0	41.0
16	38.06128208297285	40.0	37.0	41.0	33.0	41.0
17	38.10953786099782	40.0	37.0	41.0	34.0	41.0
18	37.90984324146171	39.0	37.0	41.0	33.0	41.0
19	37.74044720471306	39.0	36.0	41.0	33.0	41.0
20	37.9942657479504	40.0	36.0	41.0	34.0	41.0
21	37.86925802331809	39.0	36.0	41.0	34.0	41.0
22	38.05100574712644	40.0	36.0	41.0	34.0	41.0
23	38.08232140650105	40.0	36.0	41.0	34.0	41.0
24	37.949148745519715	40.0	36.0	41.0	34.0	41.0
25	37.584582148889716	39.0	35.0	41.0	33.0	41.0
26	37.7842175462448	39.0	36.0	41.0	33.0	41.0
27	37.87076947637293	40.0	36.0	41.0	34.0	41.0
28	37.80030538046389	40.0	36.0	41.0	34.0	41.0
29	37.87089049561241	40.0	36.0	41.0	34.0	41.0
30	37.58352129938615	40.0	36.0	41.0	33.0	41.0
31	37.47251833312734	39.0	35.0	41.0	33.0	41.0
32	37.49509743336217	40.0	36.0	41.0	33.0	41.0
33	37.43986116260866	40.0	36.0	41.0	33.0	41.0
34	37.31305359864871	40.0	35.0	41.0	33.0	41.0
35	37.310967947925676	40.0	35.0	41.0	33.0	41.0
36	37.13540765459564	40.0	35.0	41.0	32.0	41.0
37	37.158836464384294	40.0	35.0	41.0	33.0	41.0
38	36.98762513904338	39.0	35.0	41.0	32.0	41.0
39	36.939917809912245	39.0	35.0	41.0	32.0	41.0
40	36.85641195979071	39.0	35.0	41.0	32.0	41.0
41	36.64868887240967	39.0	35.0	41.0	31.0	41.0
42	36.70351058789602	39.0	35.0	41.0	31.0	41.0
43	36.72208832859556	39.0	35.0	41.0	31.0	41.0
44	36.773941210398384	39.0	35.0	41.0	32.0	41.0
45	36.76627322539447	39.0	35.0	41.0	32.0	41.0
46	36.674463395542375	39.0	35.0	41.0	31.0	41.0
47	36.59494088081407	39.0	35.0	41.0	31.0	41.0
48	36.56187687966053	39.0	35.0	41.0	31.0	41.0
49	36.561318131256954	39.0	35.0	41.0	31.0	41.0
50	36.438360009063565	39.0	35.0	41.0	31.0	41.0
51	35.55053454455568	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	12.0
10	16.0
11	10.0
12	15.0
13	13.0
14	20.0
15	31.0
16	42.0
17	85.0
18	93.0
19	165.0
20	307.0
21	495.0
22	715.0
23	1111.0
24	1560.0
25	2315.0
26	3066.0
27	3705.0
28	4072.0
29	4364.0
30	5174.0
31	6668.0
32	8745.0
33	12258.0
34	23718.0
35	31068.0
36	29539.0
37	45030.0
38	83657.0
39	120254.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93049890825197	20.19502121699007	23.153040415276234	12.72143945948173
2	33.07172578585259	25.75469657644296	23.259897828863345	17.9136798088411
3	31.302527499691013	24.645181889342066	27.51617023029704	16.53612038066988
4	28.448275862068968	24.13278127961109	27.755891319573188	19.663051538746757
5	24.949532402257653	27.860688831211633	25.491286614757136	21.69849215177358
6	25.797954517364975	33.649270794710176	25.815978659415812	14.736796028509044
7	73.96258188110247	6.763430560705311	13.70530012771392	5.56868743047831
8	72.51601573765089	7.705835702220575	12.495622708359083	7.2825258517694555
9	66.15272113047419	8.424483994561859	15.067925266757301	10.354869608206648
10	37.194877023853664	24.519527870473365	22.161970090223708	16.123625015449264
11	29.904626539776707	22.428212417088947	26.265294771968854	21.401866271165492
12	26.701736497342726	20.641504964363698	32.34998764058831	20.30677089770527
13	23.434217031269313	23.94146788612862	33.70411568409344	18.92019939850863
14	19.07752440983809	28.698811436575618	29.65718081819305	22.566483335393237
15	17.56220903884975	24.95468215712932	37.24688954805751	20.236219255963416
16	18.89007333250937	24.236806328018787	33.51048490091872	23.362635438553127
17	19.072117167222842	24.234488938326535	30.022040950850737	26.671352943599885
18	20.0673072961727	25.31670992460759	31.34449800189511	23.2714847773246
19	22.285049231656572	25.92386602397726	28.12950603551271	23.661578708853458
20	23.32967700737445	25.705001441931362	29.397118197173818	21.568203353520374
21	22.236899023606476	27.323826885840237	29.304937584970958	21.134336505582336
22	20.73085321138714	24.75899147200593	27.37326453260825	27.13689078399868
23	20.332777159807193	27.151310097639353	28.5175400650929	23.99837267746055
24	22.936235735179007	24.61145099493264	28.564402834425078	23.887910435463272
25	20.282052074321264	27.062734313846658	27.336701273019404	25.31851233881267
26	18.979421579532815	26.082478474024633	28.186925802331807	26.75117414411074
27	22.206000494376468	25.88807522761917	27.552218514398714	24.353705763605653
28	17.892823301610843	25.39781856383636	31.0715609936967	25.637797140856094
29	19.8283586701273	23.404863428500803	28.185895851357472	28.580882050014417
30	20.88998063692168	23.929880937667367	28.96814361636386	26.21199480904709
31	21.645192188851812	26.10590985869073	25.99750751864211	26.251390433815352
32	21.761834136695093	26.385026572735136	24.91837638528406	26.93476290528571
33	20.884058418819265	25.211397437481974	26.96076916738763	26.94377497631113
34	19.26343056070531	26.23079141432868	28.151907469204467	26.353870555761542
35	21.52880772875211	23.11724961891814	26.09895768961397	29.254984962715774
36	21.0122873151238	27.144615416306184	26.992955135335556	24.850142133234456
37	19.809047089358547	27.02720100523215	28.84660940139249	24.317142504016807
38	20.3636756890372	27.064021752564578	27.235766077534706	25.336536480863508
39	21.199995880196102	25.65376138095827	28.560283030527746	24.585959708317883
40	22.5453693404194	23.499618918139497	30.355230091047662	23.599781650393442
41	19.49413957895604	27.304257817327894	26.635819634985374	26.565782968730687
42	21.359638281217812	25.89451242120875	27.76207102541919	24.983778272154243
43	22.370020187039096	23.704321674288302	28.183063486178057	25.742594652494542
44	20.55241420508384	23.38349194578338	28.926945577390516	27.137148271742266
45	21.535759897828864	22.142915997198536	27.011236765129983	29.31008733984262
46	23.0961356239443	24.65522391134182	28.19439294689573	24.054247517818155
47	20.130134305607054	25.02549128661476	31.319264203023938	23.525110204754256
48	20.503491533802993	23.73650764223623	28.86798088410992	26.892019939850865
49	20.560653812878506	22.57446545544432	31.879557533061426	24.985323198615745
50	20.165925101965147	22.0875561323281	30.69511391257776	27.05140485312899
51	19.690345239566597	22.47301528447246	27.77391546162403	30.062724014336915
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	201.0
1	480.0
2	759.0
3	2261.5
4	3764.0
5	2531.0
6	1298.0
7	1320.0
8	1342.0
9	1329.0
10	1316.0
11	1301.0
12	1286.0
13	1249.0
14	1212.0
15	1223.5
16	1235.0
17	1109.0
18	983.0
19	953.5
20	924.0
21	949.0
22	974.0
23	1363.5
24	1753.0
25	1925.5
26	2532.5
27	2967.0
28	3448.0
29	3929.0
30	4412.0
31	4895.0
32	5629.5
33	6364.0
34	6893.0
35	7422.0
36	7974.5
37	8527.0
38	9457.0
39	10387.0
40	11848.0
41	13309.0
42	16337.5
43	19366.0
44	22174.5
45	24983.0
46	33466.5
47	41950.0
48	43783.5
49	45617.0
50	43561.0
51	41505.0
52	35272.0
53	29039.0
54	24942.5
55	20846.0
56	18996.5
57	17147.0
58	16743.0
59	16339.0
60	15393.0
61	14447.0
62	13241.5
63	12036.0
64	10588.0
65	9140.0
66	7679.5
67	6219.0
68	5269.0
69	4319.0
70	3837.5
71	3356.0
72	2788.5
73	2221.0
74	1835.0
75	1173.0
76	897.0
77	635.5
78	374.0
79	244.0
80	114.0
81	81.5
82	49.0
83	28.0
84	7.0
85	4.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	388368.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.44876360344504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.40793119609124	24.554045371960136
2	10.263687258718248	6.866152976331148
3	3.667480564587984	3.6801807127539785
4	1.870228281028155	2.5022729462635245
5	1.2244644405849876	2.047841080697591
6	0.9253284137204122	1.857065481965091
7	0.7613449198632718	1.7826232371633266
8	0.6224072201425775	1.6655001577301158
9	0.5653094497722292	1.701801193340239
>10	6.503642303730533	42.39980335273539
>50	0.1187667892258091	2.8053278923508245
>100	0.06015460752995526	3.9586723527370813
>500	0.0038560645852535424	0.8481763779355873
>1k	0.005398490419354959	3.3305368660359997
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	2989	0.7696308655707988	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2352	0.6056111729081696	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC	1777	0.45755572034771147	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	1746	0.44957360029662585	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	1742	0.44854364932229224	No Hit
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT	1209	0.3113026819923371	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1096	0.2822065669674123	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	967	0.24899064804515306	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC	780	0.20084043999505624	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	531	0.13672599184278827	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	509	0.13106126148395336	No Hit
ACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	501	0.12900135953528613	Illumina Single End Adapter 1 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	438	0.11277963168953156	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT	425	0.10943229102294731	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5748774358340544E-4	0.0	0.0	0.32005726527417294	0.0
2	2.5748774358340544E-4	0.0	0.0	1.7977794256993367	0.0
3	2.5748774358340544E-4	0.0	0.0	2.3794442384542496	0.0
4	2.5748774358340544E-4	0.0	0.0	3.249237836278993	0.0
5	2.5748774358340544E-4	0.0	0.0	5.865828286573559	0.0
6	2.5748774358340544E-4	0.0	0.0	7.105374284184073	0.0
7	2.5748774358340544E-4	0.0	0.0	8.378651176204013	0.0
8	2.5748774358340544E-4	0.0	0.0	10.232562930004532	0.0
9	2.5748774358340544E-4	0.0	0.0	10.951211222345817	0.0
10	2.5748774358340544E-4	0.0	0.0	12.856620524863017	0.0
11	2.5748774358340544E-4	0.0	0.0	14.894121039838504	0.0
12	5.149754871668109E-4	0.0	0.0	16.898920611378898	0.0
13	5.149754871668109E-4	0.0	0.0	17.53929262967083	0.0
14	5.149754871668109E-4	0.0	0.0	17.799355250690066	0.0
15	5.149754871668109E-4	0.0	0.0	18.357846166522474	0.0
16	5.149754871668109E-4	0.0	0.0	19.29973633255057	0.0
17	5.149754871668109E-4	0.0	0.0	20.385819634985374	0.0
18	5.149754871668109E-4	0.0	0.0	21.632575289416224	0.0
19	5.149754871668109E-4	0.0	0.0	22.36049314052651	0.0
20	5.149754871668109E-4	0.0	0.0	23.05751246240679	0.0
21	5.149754871668109E-4	0.0	0.0	23.83564042351584	0.0
22	5.149754871668109E-4	0.0	0.0	24.643379475136985	0.0
23	5.149754871668109E-4	0.0	0.0	25.431291970502205	0.0
24	5.149754871668109E-4	0.0	0.0	26.02608865817987	0.0
25	5.149754871668109E-4	0.0	0.0	26.5516211428336	0.0
26	5.149754871668109E-4	0.0	0.0	27.03389568656532	0.0
27	5.149754871668109E-4	0.0	0.0	27.53522432332221	0.0
28	5.149754871668109E-4	0.0	0.0	28.04710995756602	0.0
29	5.149754871668109E-4	0.0	0.0	28.544061302681992	0.0
30	5.149754871668109E-4	0.0	0.0	29.092252708771063	0.0
31	5.149754871668109E-4	0.0	0.0	29.610575536604458	0.0
32	5.149754871668109E-4	0.0	0.0	30.11499402628435	0.0
33	5.149754871668109E-4	0.0	0.0	30.58310674411898	0.0
34	5.149754871668109E-4	0.0	0.0	31.07233345692745	0.0
35	5.149754871668109E-4	0.0	0.0	31.587823919581428	0.0
36	5.149754871668109E-4	0.0	0.0	32.07911053433857	0.0
37	5.149754871668109E-4	0.0	0.0	32.5719420755572	0.0
38	5.149754871668109E-4	0.0	0.0	33.033360112058666	0.0
39	5.149754871668109E-4	0.0	0.0	33.483963663329625	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGAAT	35	1.209155E-7	45.0	41
CTCACGA	35	1.209155E-7	45.0	24
GCGACGT	35	1.209155E-7	45.0	17
TCACCGC	20	7.0275116E-4	45.0	19
ACCGCCA	20	7.0275116E-4	45.0	21
CGGTTGC	20	7.0275116E-4	45.0	36
CGCATCG	20	7.0275116E-4	45.0	21
GCAGTAA	20	7.0275116E-4	45.0	1
AATTGCG	20	7.0275116E-4	45.0	1
TCGCTAT	35	1.209155E-7	45.0	23
TCATGCG	20	7.0275116E-4	45.0	1
CGCAGTA	20	7.0275116E-4	45.0	13
GCGATCA	50	2.1827873E-11	45.0	9
ATGAACG	35	1.209155E-7	45.0	28
TTTGACG	20	7.0275116E-4	45.0	1
CCTATGC	80	0.0	45.0	35
TTCGCCC	20	7.0275116E-4	45.0	27
TATCGCG	20	7.0275116E-4	45.0	1
CTAGCGG	25	3.8860195E-5	45.0	2
ACGGTAG	25	3.8860195E-5	45.0	1
>>END_MODULE