##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551597_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	459844
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.976785605553186	33.0	31.0	34.0	30.0	34.0
2	32.05633432207444	33.0	31.0	34.0	30.0	34.0
3	32.00913135759083	33.0	31.0	34.0	30.0	34.0
4	35.68355572759457	37.0	35.0	37.0	33.0	37.0
5	35.74780577761154	37.0	35.0	37.0	35.0	37.0
6	35.625616513426294	37.0	35.0	37.0	33.0	37.0
7	35.977770722244934	37.0	35.0	37.0	35.0	37.0
8	35.81778168248363	37.0	35.0	37.0	35.0	37.0
9	37.66310748862658	39.0	38.0	39.0	35.0	39.0
10	37.20896434442985	39.0	37.0	39.0	34.0	39.0
11	37.25505823714129	39.0	37.0	39.0	34.0	39.0
12	37.243913153156285	39.0	37.0	39.0	34.0	39.0
13	37.34511269039066	39.0	37.0	39.0	34.0	39.0
14	38.39238959299241	40.0	38.0	41.0	34.0	41.0
15	38.485932185697756	40.0	38.0	41.0	34.0	41.0
16	38.25275093292508	40.0	38.0	41.0	34.0	41.0
17	38.27757239411626	40.0	37.0	41.0	34.0	41.0
18	38.074497003331565	40.0	37.0	41.0	34.0	41.0
19	37.89798061951444	39.0	37.0	41.0	33.0	41.0
20	38.201516166352064	40.0	37.0	41.0	34.0	41.0
21	38.056556136428874	40.0	37.0	41.0	34.0	41.0
22	38.23327693739616	40.0	37.0	41.0	34.0	41.0
23	38.287741060011655	40.0	37.0	41.0	34.0	41.0
24	38.15099251050356	40.0	37.0	41.0	34.0	41.0
25	37.77349492436566	40.0	36.0	41.0	33.0	41.0
26	38.00185062760414	40.0	37.0	41.0	34.0	41.0
27	38.10928053861745	40.0	37.0	41.0	34.0	41.0
28	38.00516914431851	40.0	37.0	41.0	34.0	41.0
29	38.06562007985317	40.0	37.0	41.0	34.0	41.0
30	37.77689390314976	40.0	36.0	41.0	33.0	41.0
31	37.69398317690347	40.0	36.0	41.0	33.0	41.0
32	37.7615517436348	40.0	37.0	41.0	33.0	41.0
33	37.73824383921504	40.0	37.0	41.0	33.0	41.0
34	37.628595784657406	40.0	37.0	41.0	33.0	41.0
35	37.61413870790964	40.0	37.0	41.0	33.0	41.0
36	37.44309157018467	40.0	36.0	41.0	33.0	41.0
37	37.47471968754621	40.0	36.0	41.0	33.0	41.0
38	37.311916649994345	40.0	36.0	41.0	33.0	41.0
39	37.26705143483442	40.0	36.0	41.0	33.0	41.0
40	37.21432485799532	40.0	36.0	41.0	32.0	41.0
41	37.0640782526248	39.0	35.0	41.0	32.0	41.0
42	37.12064526230635	40.0	35.0	41.0	32.0	41.0
43	37.10450718069606	40.0	35.0	41.0	32.0	41.0
44	37.14101738850567	40.0	35.0	41.0	33.0	41.0
45	37.12882847226451	40.0	35.0	41.0	33.0	41.0
46	37.04942110802794	39.0	35.0	41.0	32.0	41.0
47	37.01099503309818	39.0	35.0	41.0	32.0	41.0
48	36.95608945642435	39.0	35.0	41.0	32.0	41.0
49	36.92793425596506	39.0	35.0	41.0	32.0	41.0
50	36.8095810753212	39.0	35.0	41.0	32.0	41.0
51	35.983394368524976	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	16.0
10	18.0
11	13.0
12	13.0
13	24.0
14	19.0
15	24.0
16	43.0
17	65.0
18	97.0
19	186.0
20	301.0
21	531.0
22	761.0
23	1145.0
24	1746.0
25	2618.0
26	3430.0
27	4051.0
28	4217.0
29	4754.0
30	5460.0
31	7037.0
32	9356.0
33	13156.0
34	23734.0
35	31715.0
36	33467.0
37	51473.0
38	102320.0
39	157998.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.49428936769861	21.434877915119042	25.133523542766678	11.93730917441567
2	32.91833752316003	24.519402232061307	25.914223084350347	16.64803716042832
3	31.65899739911796	23.694557284644358	28.517497238193823	16.12894807804386
4	28.524891050008268	25.383825819190857	27.895112255460546	18.196170875340336
5	25.44776054488044	29.386052661337324	25.849635963500667	19.316550830281574
6	26.257817868668504	34.401014257008896	24.550282269639272	14.790885604683327
7	79.56002470403007	5.508824731865589	10.424187333095572	4.506963231008776
8	80.21307225928793	5.143265977157471	9.41862892633154	5.225032837223059
9	73.54211428223485	7.8711476065796235	11.796174354781188	6.790563756404346
10	35.411139429893616	31.69139969206948	18.630883517018816	14.266577361018085
11	26.492897591357067	25.207026730804362	29.000487121719537	19.299588556119033
12	25.106992806256034	22.225363384104174	32.463835561625245	20.203808248014543
13	23.95094858256278	24.158845173580605	33.462217621628206	18.42798862222841
14	20.904698115012916	28.059515835805186	30.446629726602936	20.589156322578962
15	19.221083671853933	26.878245665921487	33.92715790572455	19.973512756500032
16	21.323753272849054	24.77905550578022	32.93225528657545	20.964935934795278
17	21.39051504423239	25.470159445377128	29.849253225006738	23.29007228538374
18	21.596889379876654	25.41818529762267	31.071189359869866	21.913735962630806
19	22.164038239054985	27.192047737928515	29.084428632318787	21.559485390697713
20	23.428380059324468	26.770600464505357	29.34886613721175	20.45215333895843
21	22.478710171275477	27.809213559381007	29.850775480380303	19.861300788963213
22	20.985595114865042	25.454067031428053	28.640147528292204	24.92019032541471
23	20.12769547933647	27.88206435225859	29.184897486973842	22.805342681431092
24	21.27960786701577	26.051660998077608	30.363340611163785	22.305390523742837
25	20.205330503388105	27.769852384721776	28.268064821983106	23.756752289907013
26	19.615782743713087	28.19695374953245	28.840215377388855	23.34704812936561
27	21.738024199511138	26.97349535929576	28.59621958751229	22.692260853680814
28	19.502918381016173	27.137898939640397	29.85642957176782	23.502753107575614
29	19.963509364045198	25.16701316098503	28.899148406851015	25.97032906811875
30	21.20914918972521	25.745687667991753	29.04615478292638	23.99900835935665
31	20.81662476839972	28.145414531884725	26.76690355859813	24.27105714111742
32	20.842938039856996	27.32535381564183	26.91369246962013	24.918015674881048
33	21.16543871399866	25.66848757404685	28.23327041344456	24.932803298509928
34	19.428110402658294	26.154304503266324	30.23873313558511	24.17885195849027
35	19.680804794669495	24.126225415575718	29.61134645662442	26.581623333130366
36	21.23415767086229	26.102547820565235	28.837823261801827	23.825471246770643
37	20.162272422821655	26.771252859665452	30.0480162837832	23.0184584337297
38	19.9041414044763	27.315350423186995	28.933725350336204	23.846782822000502
39	21.952227277076574	24.017057958786022	30.208070563060517	23.822644201076887
40	21.61624376962622	23.523194822592007	32.57996190012265	22.28059950765912
41	20.142918033072085	24.963465871034522	30.094988735310235	24.798627360583154
42	21.24611824879742	25.726985673402286	29.374961943615663	23.65193413418464
43	22.16577795948191	25.081984325118956	28.437470098555163	24.314767616843973
44	20.753994833030333	25.65739685632519	28.808682944650794	24.779925365993684
45	20.92644462034951	23.867007071963535	28.714738041596714	26.491810266090237
46	22.327137029079427	24.87843703516845	29.424761440836456	23.369664494915668
47	19.6801523995094	25.526917824305634	31.48045859030454	23.31247118588043
48	20.504127486712886	24.515705326154087	29.606997155557103	25.373170031575924
49	20.940362383764928	23.470350814624087	31.456102504327554	24.133184297283428
50	19.84390358469394	23.024112525117214	31.26799523316603	25.86398865702282
51	19.84325118953384	23.292464400970765	29.22512852184654	27.639155887648855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	173.0
1	394.0
2	615.0
3	2480.5
4	4346.0
5	2839.0
6	1332.0
7	1326.5
8	1321.0
9	1302.5
10	1284.0
11	1330.0
12	1376.0
13	1362.0
14	1348.0
15	1316.0
16	1284.0
17	1262.5
18	1241.0
19	1280.0
20	1319.0
21	1516.5
22	1714.0
23	1738.0
24	1762.0
25	2294.0
26	3408.0
27	3990.0
28	4797.0
29	5604.0
30	6208.0
31	6812.0
32	8218.0
33	9624.0
34	10847.0
35	12070.0
36	13374.0
37	14678.0
38	15774.0
39	16870.0
40	19024.0
41	21178.0
42	23566.0
43	25954.0
44	28955.0
45	31956.0
46	38493.5
47	45031.0
48	48598.5
49	52166.0
50	50181.0
51	48196.0
52	41471.5
53	34747.0
54	30265.0
55	25783.0
56	22901.5
57	20020.0
58	18084.0
59	16148.0
60	15315.5
61	14483.0
62	12820.0
63	11157.0
64	9387.5
65	7618.0
66	6125.0
67	4632.0
68	3930.0
69	3228.0
70	2819.5
71	2411.0
72	2025.5
73	1640.0
74	1302.0
75	692.5
76	421.0
77	357.0
78	293.0
79	219.0
80	145.0
81	104.0
82	63.0
83	37.5
84	12.0
85	7.5
86	3.0
87	3.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	459844.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.05241210467533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.73645692939175	28.058500462158577
2	9.322555809499772	7.094914710638407
3	3.621409272968975	4.134100741641243
4	2.095580942240983	3.1896763845143083
5	1.4835705600188158	2.8226719168099965
6	1.2186254204313558	2.7822982019692306
7	0.9813262752383145	2.6139282284160075
8	0.8116655330048695	2.47086650824498
9	0.6928361304416238	2.372767736091593
>10	5.931416457608134	38.219941547171274
>50	0.06147012966831525	1.6098163088735469
>100	0.037341667555518564	2.2739420484658637
>500	0.004595897545294592	1.2978652126265942
>1k	0.001148974386323648	1.0587099923784058
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2926	0.6363027461486939	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	1917	0.41688050730247656	TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	844	0.18354050504083994	TruSeq Adapter, Index 20 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC	822	0.1787562738667896	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC	804	0.17484190290620297	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	800	0.17397204269273928	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	785	0.17071006689225043	No Hit
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	694	0.15092074703595132	TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	675	0.14678891102199876	TruSeq Adapter, Index 27 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	513	0.11155957237671905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19049938674854952	0.0
2	0.0	0.0	0.0	1.0131696836318405	0.0
3	0.0	0.0	0.0	1.406563965170797	0.0
4	0.0	0.0	0.0	2.008289767834309	0.0
5	0.0	0.0	0.0	3.7260462243717436	0.0
6	0.0	0.0	0.0	4.82424474386966	0.0
7	0.0	0.0	0.0	5.817407642591835	0.0
8	0.0	0.0	0.0	7.44926540304973	0.0
9	0.0	0.0	0.0	8.1610285227164	0.0
10	0.0	0.0	0.0	9.646532302259027	0.0
11	0.0	0.0	0.0	11.322535468550203	0.0
12	0.0	0.0	0.0	12.744974382616714	0.0
13	0.0	0.0	0.0	13.272979532189177	0.0
14	0.0	0.0	0.0	13.486965144701246	0.0
15	0.0	0.0	0.0	13.879272100973374	0.0
16	0.0	0.0	0.0	14.631048790459372	0.0
17	0.0	0.0	0.0	15.63269282626282	0.0
18	0.0	0.0	0.0	16.712624281278	0.0
19	0.0	0.0	0.0	17.35175407312045	0.0
20	0.0	0.0	0.0	17.927166604326686	0.0
21	0.0	0.0	0.0	18.63110098207218	0.0
22	0.0	0.0	0.0	19.333948034550847	0.0
23	0.0	0.0	0.0	20.05353989613869	0.0
24	0.0	0.0	0.0	20.645479771400737	0.0
25	0.0	0.0	0.0	21.14151755812841	0.0
26	0.0	0.0	0.0	21.596889379876654	0.0
27	0.0	0.0	0.0	22.07726968276198	0.0
28	0.0	0.0	0.0	22.546124337818913	0.0
29	0.0	0.0	0.0	23.059124398709127	0.0
30	0.0	0.0	0.0	23.589739128921984	0.0
31	0.0	0.0	0.0	24.112525117213664	0.0
32	0.0	0.0	0.0	24.587251328711478	0.0
33	0.0	0.0	0.0	25.072198397717486	0.0
34	0.0	0.0	0.0	25.52496063882534	0.0
35	0.0	0.0	0.0	26.026435051887162	0.0
36	0.0	0.0	0.0	26.501596193491707	0.0
37	0.0	0.0	0.0	26.977192265202984	0.0
38	0.0	0.0	0.0	27.440175363819034	0.0
39	0.0	0.0	0.0	27.912944389836554	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATG	35	1.2098099E-7	45.000004	1
AGGGCGC	70	0.0	45.000004	6
ACGTTAG	25	3.8871425E-5	45.0	1
TAAACGC	20	7.028868E-4	45.0	35
CTAACGG	20	7.028868E-4	45.0	2
AATTGCG	20	7.028868E-4	45.0	1
ACTCGTT	20	7.028868E-4	45.0	18
TCCCGCG	25	3.8871425E-5	45.0	16
ACCCGGT	20	7.028868E-4	45.0	10
CGTTTTT	1385	0.0	42.56318	1
GGCCGAT	115	0.0	41.08696	8
GGGCGTC	55	6.002665E-11	40.909092	7
TCAAGCG	45	1.9239451E-8	40.0	17
GTACGAG	45	1.9239451E-8	40.0	1
GCGTCCC	80	0.0	39.375	7
GCGTAAG	40	3.4521872E-7	39.375	1
CTCACGT	35	6.240114E-6	38.57143	45
TTACTAG	35	6.240114E-6	38.57143	1
ATTGCGG	70	0.0	38.57143	2
CATATTA	95	0.0	37.894737	28
>>END_MODULE