FastQCFastQC Report
Sat 18 Jun 2016
SRR3551596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424688
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG42981.0120370719210336No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC40160.9456353840937346No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC34350.8088290698112497No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25060.5900802471461403No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC16640.3918170515766869Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT10390.24465019025731832No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT8600.20250160117545116No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC8260.1944957239196775Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA7310.17212636099913348No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT6270.14763779527559054No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCC5660.13327430961082018No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC5630.1325679086764872No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG4940.1163206871868289No Hit
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT4900.11537881927438495Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCA302.1622564E-645.00000416
TTCACGG302.1622564E-645.0000042
CGTTCAT302.1622564E-645.00000417
CCGGTAT302.1622564E-645.00000442
CGGTATT302.1622564E-645.00000443
TCGTTTA351.2095006E-745.038
CGCATCG351.2095006E-745.021
GTTAACG207.028258E-445.01
CCGCGTA207.028258E-445.040
CGGTCGA406.7975634E-945.041
ATCGCAT207.028258E-445.010
AATGGGC1100.042.9545444
ACGGGAC700.041.7857135
GTCGAAT501.0786607E-940.543
GCTACGA501.0786607E-940.510
CCGATGA3950.040.4430418
TACGGCT4150.040.120487
GGTCGAA451.9233994E-840.00000442
TAACGCC451.9233994E-840.00000412
CGAATGC451.9233994E-840.00000445