##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551596_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 424688 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06235636514335 33.0 31.0 34.0 30.0 34.0 2 32.15811372113175 33.0 31.0 34.0 30.0 34.0 3 32.120606186188446 33.0 31.0 34.0 30.0 34.0 4 35.76891270768188 37.0 35.0 37.0 35.0 37.0 5 35.80085380326263 37.0 35.0 37.0 35.0 37.0 6 35.677480880081376 37.0 35.0 37.0 35.0 37.0 7 35.95932072486155 37.0 35.0 37.0 35.0 37.0 8 35.71071468937196 37.0 35.0 37.0 35.0 37.0 9 37.50418895754059 39.0 37.0 39.0 35.0 39.0 10 37.23680207587688 39.0 37.0 39.0 34.0 39.0 11 37.31718814753419 39.0 37.0 39.0 34.0 39.0 12 37.18390441924424 39.0 37.0 39.0 34.0 39.0 13 37.23731539388916 39.0 37.0 39.0 34.0 39.0 14 38.324259220886866 40.0 38.0 41.0 34.0 41.0 15 38.37117837094526 40.0 38.0 41.0 34.0 41.0 16 38.193664525486945 40.0 37.0 41.0 34.0 41.0 17 38.160404814828766 40.0 37.0 41.0 34.0 41.0 18 37.97240797950496 40.0 37.0 41.0 33.0 41.0 19 37.80293297667935 39.0 37.0 41.0 33.0 41.0 20 37.980635195720154 40.0 36.0 41.0 34.0 41.0 21 37.90908619975135 40.0 36.0 41.0 34.0 41.0 22 38.04921024375542 40.0 37.0 41.0 34.0 41.0 23 38.05104217684512 40.0 36.0 41.0 34.0 41.0 24 37.92999095806804 40.0 36.0 41.0 34.0 41.0 25 37.628112873450625 39.0 35.0 41.0 33.0 41.0 26 37.79321996383227 40.0 36.0 41.0 33.0 41.0 27 37.83704036845873 40.0 36.0 41.0 33.0 41.0 28 37.737663885016765 40.0 36.0 41.0 33.0 41.0 29 37.78987868741288 40.0 36.0 41.0 33.0 41.0 30 37.53029753607354 40.0 36.0 41.0 33.0 41.0 31 37.46000828843763 40.0 35.0 41.0 33.0 41.0 32 37.39599659043816 40.0 35.0 41.0 33.0 41.0 33 37.34287994951588 40.0 35.0 41.0 33.0 41.0 34 37.138715951474964 40.0 35.0 41.0 32.0 41.0 35 37.1113876539954 40.0 35.0 41.0 32.0 41.0 36 36.93576225370154 40.0 35.0 41.0 32.0 41.0 37 36.890140526692534 40.0 35.0 41.0 32.0 41.0 38 36.761768639565986 39.0 35.0 41.0 31.0 41.0 39 36.67317418905173 39.0 35.0 41.0 31.0 41.0 40 36.626631786158306 39.0 35.0 41.0 31.0 41.0 41 36.414857495384844 39.0 35.0 41.0 30.0 41.0 42 36.46141167162717 39.0 35.0 41.0 31.0 41.0 43 36.42827204912783 39.0 35.0 41.0 31.0 41.0 44 36.490861526579515 39.0 35.0 41.0 31.0 41.0 45 36.474020928305016 39.0 35.0 41.0 31.0 41.0 46 36.39822410805109 39.0 35.0 41.0 31.0 41.0 47 36.28963615642542 39.0 35.0 41.0 31.0 41.0 48 36.242733489055496 39.0 35.0 41.0 31.0 41.0 49 36.234266096522624 39.0 35.0 41.0 31.0 41.0 50 36.08026598349847 38.0 35.0 41.0 31.0 41.0 51 35.20068615077422 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 9.0 9 19.0 10 18.0 11 19.0 12 15.0 13 29.0 14 29.0 15 26.0 16 59.0 17 75.0 18 133.0 19 235.0 20 553.0 21 922.0 22 1341.0 23 1721.0 24 2268.0 25 2912.0 26 3668.0 27 4351.0 28 4304.0 29 4835.0 30 5556.0 31 7015.0 32 9261.0 33 13142.0 34 25173.0 35 33008.0 36 31945.0 37 48500.0 38 88295.0 39 135176.0 40 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.2861865651961 19.44392118449309 22.4524827638172 12.817409486493613 2 33.17847455072901 27.618392796594204 22.4331744716121 16.769958181064688 3 31.028425573597556 26.84818031119316 25.630580567381227 16.492813547828053 4 27.782513280337568 23.5966168104585 29.860509362167047 18.760360547036882 5 29.069811249670348 27.0633971291866 23.775571713822856 20.0912199073202 6 25.622810157103572 36.018206306747544 24.096748671966242 14.262234864182647 7 74.65197980635196 5.988867121274912 14.556568586821383 4.802584485551746 8 74.05530648381871 9.678163734317899 10.87245224729684 5.394077534566553 9 68.0191104999435 7.9434785065742375 12.553451004031194 11.48395998945108 10 39.92366160569642 23.875409712541913 19.808895000565123 16.39203368119655 11 32.481256828542364 22.83040726368534 24.587697321327656 20.100638586444635 12 27.389283426892213 20.35612025769506 30.23184078664808 22.022755528764645 13 26.118939079983424 22.466610782503864 31.95004332592397 19.46440681158874 14 21.278679877933918 28.91087103944543 28.314904117846513 21.49554496477414 15 19.28521644124628 24.20977282145952 34.9051539012169 21.599856836077308 16 21.35073277323588 22.788023207625365 31.58530497683005 24.275939042308707 17 21.39288136231775 24.1815167840862 30.083732057416267 24.341869796179783 18 21.970246392645894 23.864107297592586 29.94362920544023 24.22201710432129 19 24.01527709753984 24.929595373544814 27.5046622461666 23.550465282748746 20 26.664987002222805 24.560147684888673 28.833166936668803 19.94169837621972 21 24.055541950796822 27.429783747127303 27.851505104924083 20.66316919715179 22 22.598943224202237 23.723298044682213 28.220246392645898 25.457512338469652 23 23.521502844441095 26.53029989074332 28.084381946275855 21.86381531853973 24 23.663960366198246 24.872141430885733 27.38857702595788 24.07532117695814 25 22.34440342086426 27.611564254228988 26.512168933428775 23.531863391477977 26 21.06770146554647 25.379337301736804 29.650472817692048 23.902488415024678 27 24.189287194363864 24.987049316203898 28.972092453754282 21.851571035677956 28 21.209217119391177 26.027342425498247 30.307190219643598 22.456250235466978 29 22.83299740044456 23.975483178239084 30.739036657499152 22.4524827638172 30 22.545963154127264 25.426195230380895 29.547573748257545 22.4802678672343 31 25.284208642579962 24.55119993972045 25.683325170478096 24.48126624722149 32 25.497306257770408 26.511462532494445 27.2432939004634 20.747937309271748 33 24.913819086011376 24.256866217081715 25.508608672719742 25.320706024187167 34 23.690097200768566 25.3235316279245 27.993491692725016 22.992879478581923 35 24.453481143804392 23.86316542968014 27.81147571864522 23.871877707870247 36 25.82766642806013 26.068549146667667 26.603294653957725 21.50048977131447 37 24.191641864144973 25.726886561428625 29.517904909015556 20.563566665410843 38 24.358587951625665 25.640470180461893 24.835173115322306 25.165768752590132 39 24.537071921033792 25.279970236973963 27.334184154014242 22.848773687977996 40 23.351024752288737 23.78687412877218 28.90616169988321 23.955939419055873 41 23.09907508570998 26.115171608333647 27.01583279960818 23.769920506348193 42 24.88556304863806 24.579455977093772 28.49197528538598 22.043005688882193 43 25.260426477790755 23.750847681121197 27.412830124703312 23.575895716384736 44 23.707992691104998 24.009390423087066 28.641261349508344 23.64135553629959 45 23.103313491315976 23.324652450740306 27.68620728629017 25.88582677165354 46 25.42360509362167 24.853304072636853 27.03467015785706 22.688420675884412 47 22.003447236559545 25.136099913348154 30.96014014994537 21.90031270014693 48 23.14169460874807 24.57192103379422 27.710931318991825 24.575453038465884 49 22.853247560562107 22.891157744037976 30.56502656067513 23.690568134724785 50 22.757177033492823 22.710319104848736 29.45409335794748 25.078410503710955 51 22.483328937949743 23.44897901518291 27.398702106016653 26.668989940850697 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 135.0 1 531.5 2 928.0 3 2542.5 4 4157.0 5 2838.5 6 1520.0 7 1507.0 8 1494.0 9 1411.0 10 1328.0 11 1341.5 12 1355.0 13 1294.5 14 1234.0 15 1220.0 16 1206.0 17 1182.5 18 1159.0 19 1257.5 20 1356.0 21 1356.5 22 1357.0 23 1628.5 24 1900.0 25 2027.0 26 2799.0 27 3444.0 28 3886.0 29 4328.0 30 4948.0 31 5568.0 32 6099.0 33 6630.0 34 7644.5 35 8659.0 36 9091.0 37 9523.0 38 10507.0 39 11491.0 40 13542.0 41 15593.0 42 17515.0 43 19437.0 44 21660.0 45 23883.0 46 34865.0 47 45847.0 48 42757.5 49 39668.0 50 38480.0 51 37292.0 52 33252.0 53 29212.0 54 26735.5 55 24259.0 56 23143.0 57 22027.0 58 21474.5 59 20922.0 60 19879.5 61 18837.0 62 17687.5 63 16538.0 64 14598.5 65 12659.0 66 10839.5 67 9020.0 68 7883.5 69 6747.0 70 5467.0 71 4187.0 72 3680.0 73 3173.0 74 2666.0 75 1728.0 76 1297.0 77 890.0 78 483.0 79 393.5 80 304.0 81 205.0 82 106.0 83 86.5 84 67.0 85 48.5 86 30.0 87 17.5 88 5.0 89 4.5 90 4.0 91 4.0 92 4.0 93 2.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 424688.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.02520070454787 #Duplication Level Percentage of deduplicated Percentage of total 1 73.05378125791334 23.395620070108123 2 8.2457891978493 5.281461080570331 3 3.2045983562011755 3.0788371660442047 4 1.8716153702434422 2.3975543149505163 5 1.418489493670935 2.2713705366052084 6 1.1095083592925592 2.131933673383069 7 0.9886996039430931 2.2164312276949176 8 0.8296411382505271 2.1255539172178137 9 0.8228942204418283 2.37180173114358 >10 8.333217446466753 45.6439193357238 >50 0.06936946811116833 1.528393676180151 >100 0.04354041083573331 2.563078617314634 >500 0.004427838390074574 0.9862357833152575 >1k 0.004427838390074574 4.007808869748415 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG 4298 1.0120370719210336 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC 4016 0.9456353840937346 No Hit GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC 3435 0.8088290698112497 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2506 0.5900802471461403 No Hit GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC 1664 0.3918170515766869 Illumina Single End Adapter 1 (95% over 21bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT 1039 0.24465019025731832 No Hit GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT 860 0.20250160117545116 No Hit CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC 826 0.1944957239196775 Illumina Single End Adapter 1 (95% over 21bp) GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA 731 0.17212636099913348 No Hit GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT 627 0.14763779527559054 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCC 566 0.13327430961082018 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC 563 0.1325679086764872 No Hit CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG 494 0.1163206871868289 No Hit CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT 490 0.11537881927438495 Illumina Single End Adapter 1 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.11985269185849376 0.0 2 0.0 0.0 0.0 0.848622989112007 0.0 3 0.0 0.0 0.0 1.1050465282748747 0.0 4 0.0 0.0 0.0 1.6082394604980597 0.0 5 0.0 0.0 0.0 3.3798930038051465 0.0 6 0.0 0.0 0.0 4.094770749350111 0.0 7 0.0 0.0 0.0 4.884762460912482 0.0 8 0.0 0.0 0.0 5.856534679576536 0.0 9 0.0 0.0 0.0 6.2217439626266815 0.0 10 0.0 0.0 0.0 7.995987642692989 0.0 11 0.0 0.0 0.0 9.162491052254833 0.0 12 0.0 0.0 0.0 11.02738951889387 0.0 13 0.0 0.0 0.0 11.467006367027087 0.0 14 0.0 0.0 0.0 11.66738876539954 0.0 15 0.0 0.0 0.0 12.140206457446407 0.0 16 0.0 0.0 0.0 12.765842218287307 0.0 17 0.0 0.0 0.0 13.443516181290736 0.0 18 0.0 0.0 0.0 14.169460874806918 0.0 19 0.0 0.0 0.0 14.836538823795351 0.0 20 0.0 0.0 0.0 15.258731115548356 0.0 21 0.0 0.0 0.0 15.71624345401801 0.0 22 0.0 0.0 0.0 16.233799871905966 0.0 23 0.0 0.0 0.0 16.718390912858382 0.0 24 0.0 0.0 0.0 17.132812794333724 0.0 25 0.0 0.0 0.0 17.48483592660965 0.0 26 0.0 0.0 0.0 17.80601288475304 0.0 27 0.0 0.0 0.0 18.16910296500019 0.0 28 0.0 0.0 0.0 18.50982368232679 0.0 29 0.0 0.0 0.0 18.843715857288174 0.0 30 0.0 0.0 0.0 19.20680593753532 0.0 31 0.0 0.0 0.0 19.558358135855027 0.0 32 0.0 0.0 0.0 19.907555664393627 0.0 33 0.0 0.0 0.0 20.252043853370004 0.0 34 0.0 0.0 0.0 20.601947782842934 0.0 35 0.0 0.0 0.0 20.958209320724862 0.0 36 0.0 0.0 0.0 21.320357533059564 0.0 37 0.0 0.0 0.0 21.66202011829861 0.0 38 0.0 0.0 0.0 21.983668010398222 0.0 39 0.0 0.0 0.0 22.322034057943714 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTCA 30 2.1622564E-6 45.000004 16 TTCACGG 30 2.1622564E-6 45.000004 2 CGTTCAT 30 2.1622564E-6 45.000004 17 CCGGTAT 30 2.1622564E-6 45.000004 42 CGGTATT 30 2.1622564E-6 45.000004 43 TCGTTTA 35 1.2095006E-7 45.0 38 CGCATCG 35 1.2095006E-7 45.0 21 GTTAACG 20 7.028258E-4 45.0 1 CCGCGTA 20 7.028258E-4 45.0 40 CGGTCGA 40 6.7975634E-9 45.0 41 ATCGCAT 20 7.028258E-4 45.0 10 AATGGGC 110 0.0 42.954544 4 ACGGGAC 70 0.0 41.785713 5 GTCGAAT 50 1.0786607E-9 40.5 43 GCTACGA 50 1.0786607E-9 40.5 10 CCGATGA 395 0.0 40.44304 18 TACGGCT 415 0.0 40.12048 7 GGTCGAA 45 1.9233994E-8 40.000004 42 TAACGCC 45 1.9233994E-8 40.000004 12 CGAATGC 45 1.9233994E-8 40.000004 45 >>END_MODULE