FastQCFastQC Report
Sat 18 Jun 2016
SRR3551593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684798
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54250.7922044164848612No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC26130.38157237608754696TruSeq Adapter, Index 20 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC24140.35251271177777976No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG20580.30052657864070864No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC20030.2924950131279589No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC10880.1588789686885768No Hit
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT9790.14296186612694547TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC7840.1144863156726509TruSeq Adapter, Index 20 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA650.045.00000431
CTATGCG253.889148E-545.01
ATTAACG207.031287E-445.01
CCTCGCG253.889148E-545.040
TCGTCGG207.031287E-445.033
AATAGCG207.031287E-445.01
TATCGCG253.889148E-545.01
GTACGAT253.889148E-545.026
AATACGG302.1642118E-644.9999962
CGTTTTT23750.042.0631561
ACGGGTC501.0804797E-940.55
CTACGAA1000.040.511
GGCGATA1950.040.3846178
TAGCGCG451.9263098E-840.01
TACGTAG403.4554978E-739.3751
ACATACG1200.039.37499617
TAAGCGG1100.038.863642
TCGGACC356.2445906E-638.57142628
GCGATAT356.2445906E-638.5714269
CCGCCTA356.2445906E-638.57142629